1GMI


Conserved Protein Domain Family
C2_PKC_epsilon

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cd04014: C2_PKC_epsilon 
Click on image for an interactive view with Cn3D
C2 domain in Protein Kinase C (PKC) epsilon
A single C2 domain is found in PKC epsilon. The PKC family of serine/threonine kinases regulates apoptosis, proliferation, migration, motility, chemo-resistance, and differentiation. There are 3 groups: group 1 (alpha, betaI, beta II, gamma) which require phospholipids and calcium, group 2 (delta, epsilon, theta, eta) which do not require calcium for activation, and group 3 (xi, iota/lambda) which are atypical and can be activated in the absence of diacylglycerol and calcium. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. Members here have a type-II topology.
Statistics
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PSSM-Id: 175981
View PSSM: cd04014
Aligned: 22 rows
Threshold Bit Score: 175.539
Threshold Setting Gi: 167524777
Created: 27-Dec-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
metal binding
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:metal binding pocket [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                             #                                          
1GMI_A         3 VFNGLLKIKICEAVSLKPtawslrda-vgprpqtFLLDPYIALNVDDSRIGQTATKQKtNSPAWHDEFVTDvcnGRKIEL 81  black rat
CAF93761       3 VFSGLLKVRVCEAVDLKPtpwalrh--avgksgsFLLDPYLALNLDQTRLGQTATRTKtNSPAWHQEFCTEvreGRSLEL 80  Tetraodon nigro...
CAH68918       2 KFNGYLRLHIREAVDLKPtrfstr----htfnrkIQLDPYIVVKVDNVKHAKTATKPKtNKPTFNEEFCPYisnGVELEL 77  zebrafish
AAH73353       2 RFNGSLKLKIGEAVELQPtrwsmrh--svfkkgsYLLDPYITINVDECRIGQTSIKHRtNKPTYNEEFCVTvseGSKVEL 79  African clawed ...
XP_001627685   3 GFTGSLKLRIFEAIELKPaakah------wgvklTTIDPYLLVKVDDVQIAQTTSKTKtFTPVWNEEFIVEvenGRDLEF 76  starlet sea ane...
2065190        7 GFTGFMKIRILEALDLNKtnvksqf---vtsmkvSSLDPYLQAYVDDQVIAKTSKKTAtYCPVWEEDFSIDlhkAKMLSL 83  Hydra vulgaris
CAX12684       3 VFNGLLKIKICEALDLKPtawslrha-vgpktqtFLLDTYIALNVDDFRVGQTSTKQKtNSPAWHDELVTEvhdGRKIEL 81  zebrafish
XP_419464      3 VFNGLLKIKICEAVNLKPtawslrha-vgprpqtFLLDPYIALNVDDSRIGQTSTKQKtNSPAWNDEFVTDvcnGRKIEM 81  chicken
XP_002573210   3 YFSGTLNIRIYEAAELKPtacatrhavgpaaklyELLDPYVTIDVDDNAVGKSSTKSRtNIPQWNEDICARvnyAQQLTF 82  Schistosoma man...
XP_002610675   3 VFNGLIKVRVCEAQDLRPtdyatrh--avvrpasQVLDPYVKLNVDDTCFGQTTAKPKtLNPKWKEDFTTEvhnGHTVGV 80  Florida lancelet
Feature 1            #     #                                                  
1GMI_A        82 AVFHDAPIGYDDFVANCTIQFEELLqn--gsRHFEDWIDLE-----PEGKVYVIIDLSGSS 135 black rat
CAF93761      81 SVFHDAPIGYDDFVANCTIQLEDLLqn--gtRHYEDWVRSSancwmEISEIFSLILCEAPD 139 Tetraodon nigroviridis
CAH68918      78 AVFHSTRLGYDTFVANCRIQLKEITttpgtkPKFEGWVNLE-----PEGSVYIFITLTGSF 133 zebrafish
AAH73353      80 AVFHETPIGYDDFVAHCTLSFEELIkgcattDSFEGWVNLE-----PEGKLFVVITLIGTF 135 African clawed frog
XP_001627685  77 AVFHNAIMPPKPFIANTTVSLEEVIk----sGSSEIWIPLE-----PHGQVHVNIELKPSN 128 starlet sea anemone
2065190       84 TVFHSSIVGSDSFVANSSVLIQDIId----eGQSDLWIQLE-----PAGKLHIFIELKSGE 135 Hydra vulgaris
CAX12684      82 SVFHDAPIGYDDFVANCTIQFEELLhn--gsKHFEDWIDLE-----PEGKVFVIIDLSGSS 135 zebrafish
XP_419464     82 AVFHDAPIGYDDFVANCTIQFEDLLqn--gsRHFEDWIDLE-----PEGRVYVIIDLSGSS 135 chicken
XP_002573210  83 TVYHDAAIPPDDFVAIVELALRDVR------FGEDMWLELE-----PQGKLLIRIDLAGTR 132 Schistosoma mansoni
XP_002610675  81 TVFHNTTIGPDDFVANCNVTFEELLqe--qgGSADIWVDLE-----PAGKVHLIVELKGAA 134 Florida lancelet

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