Conserved Protein Domain Family
C2B_RasA3

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cd04010: C2B_RasA3 
C2 domain second repeat present in RAS p21 protein activator 3 (RasA3)
RasA3 are members of GTPase activating protein 1 (GAP1), a Ras-specific GAP, which suppresses Ras function by enhancing the GTPase activity of Ras proteins resulting in the inactive GDP-bound form of Ras. In this way it can control cellular proliferation and differentiation. RasA3 contains an N-terminal C2 domain, a Ras-GAP domain, a plextrin homology (PH)-like domain, and a Bruton's Tyrosine Kinase (BTK) zinc binding domain. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the second C2 repeat, C2B, and has a type-I topology.
Statistics
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PSSM-Id: 175977
View PSSM: cd04010
Aligned: 13 rows
Threshold Bit Score: 162.952
Threshold Setting Gi: 156390904
Created: 11-Jan-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative Ca2+
Feature 1:putative Ca2+ binding pocket [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                #             #                                                          
BAA11230      172 QLVVHIKACHGLPli-nGQSCDPYATVSLVGps-rnDQKKTKVKKKaSNPQFNEIFYFEvtrss---------------- 233  human
AAH77473       55 KLLTRVLECQGLPi--vNGQCDPYATVTLLGps-rsEAKKTKVRKKtNNPQFDEVFYFEvtkpc---------------- 115  African clawe...
CAG32764      144 QLVVHIKECHGLPli-nGQNCDPYATVSLVGps-rnDQKKTKVKKKtSNPQFNETFYFEvtrss---------------- 205  chicken
CAF95517       48 KLATRVLECQGLPi--iNGQCDPYAAVSLLGps-rsDAKKTKVKRKtNNPQFEEVFYFEvtrp----------------- 107  Tetraodon nig...
P48423        280 GVTIKVPACVDLAk--kQGTCDPFVVCTAHYsnkhqVTRRTKQRKKtVDPEFDEAMYFDlhidadagstnttgsnksags 357  fruit fly
XP_395469     145 KLTVRVIECSELTi--kNGGCDPFAIVTVIYsngkqVLKRTKIKKKtVSPYFNETFIFEpeit----------------- 205  honey bee
XP_967172     151 RLSVQVVKCLDLTl--kNGACDPYAVACVTYtnrktVSKRTKVRKKtTCPQFDEIFIFDgy------------------- 209  red flour beetle
EEB14485      144 KVVVRLLECSDLTl--kGGSCDPFATVTMVYsngkqDLKRTKVKKKtNSPKFDETFTFEvcqn----------------- 204  human body louse
EEC04328      151 KLSVRVTECSDLNl--vNGSCNPYALVTLCHglvkqETRKSKVRRKtVCPRFEDAFYFDv-------------------- 208  black-legged ...
XP_001943934  132 RLGVRLNEACELAi--tNGNCDPFADVTVRYtndktETQRTKVKKKtNSPVFDEAFAFTlpgl----------------- 192  pea aphid
XP_307920     256 QIKIKLTECVELAr--kNGLCDPYAVVAAHYsnkktITKRTKVRKKtINPSFDETFCFDlcidtcgg-----------ds 322  Anopheles gam...
XP_001635509  151 KLSIRIIECSELFac-sGGVSDSYVSVIMIT-----PVSRSEPKKT-RVKKKTVNPQFDe-------------------- 203  starlet sea a...
XP_002111334   93 KLHVKVIEATDLHacqsSGTSDTFASVKLMNy----TTNFDNVILF-NGYRQIKAFIPPfilr----------------- 150  Trichoplax ad...
Feature 1                      # #                     # #                                        
BAA11230      234 --sytrKSQFQVEEEDIEKLEIRIDLWNNGnl--vqDVFLGEIKVPVNVlrtd-------sshQAWYLLQPrd------- 295  human
AAH77473      116 --synkKSHFEIEEDDVDKLEIRVDLWNASnlk-fgDEFLGELKLPLKVlrqs-------schGAWYFLQPrd------- 178  African clawe...
CAG32764      206 --sytkKSQFQVEEEDIEKLEVRVDLWNNGnl--vqDIFLGEIKVPVKVlrn--------dsfQAWYLLQPre------- 266  chicken
CAF95517      108 --lsytKRQFDVEEEDVDKLTLRVDLWNASnlk-fgDEFLGGVRVPLRVlgqa-------gvhDAWYFLQPrd------- 170  Tetraodon nig...
P48423        358 lessanKGYSIYPVGGADLVEIVVSVWHDAhgamsdKVFLGEVRLPMLNkqeqq-----avnpSAWYYLQPrsmthssrs 432  fruit fly
XP_395469     206 --eskeKDISHYSLEHGEVNEVVVGLWHASsgmgeqPAFLGEVRITLRGlqkqs-----tnstTAWYFLQPravkhrp-- 276  honey bee
XP_967172     210 -----eGRDKDMCGEEVEICELNVSIWHDApgm-gdDVFLGEVRVQLSGiqqqn-----aaprNAWYFLQPrsaknrps- 277  red flour beetle
EEB14485      205 --hdrdTYQVHNTERDVEFSEVLVTLWHDSpgm-sdNLFLGEVRIPVRVhviqq-----ygvnNAWYYLQPrsnksrsss 276  human body louse
EEC04328      209 ------RKGFNMPRVGACRVCLQHDLSGVGgm--fgSAFLGEVRIPLREldle------rgshQAWYLLQPrdqtqqqt- 273  black-legged ...
XP_001943934  193 --sgihKQHIAGQTTTADWAELIVTLYHEIss--snTVFLGQVTIPLQGrt-----------vSAWYFLQPrtrks---- 253  pea aphid
XP_307920     323 aksdsnNMYTVMPLGGADLCEVVISFKHASpgi-gdDVFLGEIRIPVRGkqqqn-----avqpSAWYFLQPrtsqsrpm- 395  Anopheles gam...
XP_001635509  204 ------TFLFEIPSEDLESRVLRCAVWSPSml--geDIFLGEVRIPLNKcdls-------ivhEGWYWLGPree------ 262  starlet sea a...
XP_002111334  151 --nrpeVKRTKLWDNDLSNYCVNIELCNANmi---gDTFMGKVTIPLQSldlsssheasqlifQNSYMLSEre------- 218  Trichoplax ad...
Feature 1                                       
BAA11230      296 -----------ngNKSSKTDDLGSLRLNIC 314  human
AAH77473      179 ------------nGKSVKPDDLGSLRLNVV 196  African clawed frog
CAG32764      267 -----------ngNKSSKTDDLGSLRLNIC 285  chicken
CAF95517      171 -----------ngGKSVKVEELGSLRLNIV 189  Tetraodon nigroviridis
P48423        433 lnatprscatppgTRLSVDSTIGSLRLNLN 462  fruit fly
XP_395469     277 ------nkisnssTSPGVLPGLGSLRLKIH 300  honey bee
XP_967172     278 -----ftystppgTRLSKDNSLGSLRLQVQ 302  red flour beetle
EEB14485      277 ----ksqsvnstlKSTNNLNELGSIRLKMN 302  human body louse
EEC04328      274 -----qqqtqqaqQTQATTSPLGSLRLKLF 298  black-legged tick
XP_001943934  254 ---------slreDQGFNYPSVGSLRLKIH 274  pea aphid
XP_307920     396 -----rtcatppgTRLSCDNSLGSLRLKLN 420  Anopheles gambiae str. PEST
XP_001635509  263 -----------rdSPAQRQGSIGSLRLKVC 281  starlet sea anemone
XP_002111334  219 ------------gRHSGNKADLGSLRLKIN 236  Trichoplax adhaerens

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