2BRS


Conserved Protein Domain Family
CLECT_EMBP_like

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cd03598: CLECT_EMBP_like 
Click on image for an interactive view with Cn3D
C-type lectin-like domain (CTLD) of the type found in the human proteins, eosinophil major basic protein (EMBP) and prepro major basic protein homolog (MBPH)
CLECT_EMBP_like: C-type lectin-like domain (CTLD) of the type found in the human proteins, eosinophil major basic protein (EMBP) and prepro major basic protein homolog (MBPH). CTLD refers to a domain homologous to the carbohydrate-recognition domains (CRDs) of the C-type lectins. Eosinophils and basophils carry out various functions in allergic, parasitic, and inflammatory diseases. EMBP is stored in eosinophil crystalloid granules and is released upon degranulation. EMBP is also expressed in basophils. The proform of EMBP is expressed in placental X cells and breast tissue and increases significantly during human pregnancy. EMBP has cytotoxic properties and damages bacteria and mammalian cells, in vitro, as well as, helminth parasites. EMBP deposition has been observed in the inflamed tissue of allergy patients in a variety of diseases including asthma, atopic dermatitis, and rhinitis. In addition to its cytotoxic functions, EMBP activates cells and stimulates cytokine production. EMBP has been shown to bind the proteoglycan heparin. The binding site is similar to the carbohydrate binding site of other classical CTLD, such as mannose-binding protein (MBP1), however, heparin binding to EMBP is calcium ion independent. MBPH has reduced potency in cytotoxic and cytostimulatory assays compared with EMBP.
Statistics
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PSSM-Id: 153068
View PSSM: cd03598
Aligned: 7 rows
Threshold Bit Score: 161.85
Threshold Setting Gi: 47226918
Created: 22-Mar-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
carbohydrate
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:carbohydrate binding site [chemical binding site]
Evidence:
  • Structure:2BRS_B, Homo sapiens, EMBP bound with heparin disaccharide, contacts at 3.5 A
    View structure with Cn3D
  • Comment:Heparin recognition of EMBP does not require Ca2+ coordination.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
2BRS_B      1 TCRYLLVrs-lQTFSQAWFTCRrc-yrGNLVSIHNfNINYRIQcsv---saLNQGQVWIGGritgsgrcRRFQWVDGSRW 75  human
P22032    115 TRGYVMVgs-aRTFNEAQWVCQrc-yrGNLASIHSfAFNYQVQcts---agLNVAQVWIGGqlrgkgrcRRFVWVDRTVW 189 domestic guinea pig
XP_876321  49 KSCRHLMvqtpNIFRKALSICKkc-ygGNLISIHNlLFDNCISrwa---rrINQAKVWIGViiklwsiyKTFRWTDGSRW 124 cattle
XP_598643 101 KRCYYRFvrtpGRFRHAHRICRrc-yrGNLVSIHSyRFDYRVTrws---rrTNQSQVWIGGfirgrsrcRRFRWTDGSHW 176 cattle
XP_421065  36 GRCFAVVka-rRTYRAAQCYCRkv-ykGQLASVHStATNEQLRkla---atYTYCSLWIGAvttckggsWKTSWQDLSSW 110 chicken
CAG01003  227 GRCYEFNpt-pLAFQDAQVMCRglapkAELASIPNnDVHMRLVslvtnggtSNPVLTWIGGive--aknQQPSWVDGSVW 303 Tetraodon nigrovir...
CAG05810   70 NRCYRFFkg-pRRGDDAEVFCRrlspeGHLASITSqHLHQEVLhlvq-rsnGALTRFWVGGsry--lksSRFIWLDGSPW 145 Tetraodon nigrovir...
Feature 1              #                    #  #           
2BRS_B     76 NFAYWAAHQPws--rgGHCVALCtr-gGYWRRAHCLRRLPFICSY 117 human
P22032    190 NFAYWARGQPwggrqrGRCVTLCar-gGHWRRSHCGKRRPFVCTY 233 domestic guinea pig
XP_876321 125 NFGYWARGQPgn--gkGHCVDLStk-gGHWRRASCKWRLPFICSY 166 cattle
XP_598643 177 NFGYWAQGQPgn--grGHCITLCtr-gGHWQRASCKRRLPFVCAF 218 cattle
XP_421065 111 NYANWAIGQPcr--rfRTCTVLSpk-dGLWRSRACFLLRPFICQY 152 chicken
CAG01003  304 KYSDWMPDEPsihtddRACVEMFktdeSWWAAQHCDLRRASICSY 348 Tetraodon nigroviridis
CAG05810  146 VYSAWQPGRPrptsavENCVEVLv--nGRFNDKRCSEAQAFVCSH 188 Tetraodon nigroviridis

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