1HV9,2OI6,2OI7,1HM9


Conserved Protein Domain Family
LbH_GlmU_C

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cd03353: LbH_GlmU_C 
Click on image for an interactive view with Cn3D
N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU), C-terminal left-handed beta-helix (LbH) acetyltransferase domain: GlmU is also known as UDP-N-acetylglucosamine pyrophosphorylase. It is a bifunctional bacterial enzyme that catalyzes two consecutive steps in the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), an important precursor in bacterial cell wall formation. The two enzymatic activities, uridyltransferase and acetyltransferase, are carried out by two independent domains. The C-terminal LbH domain possesses the acetyltransferase activity. It catalyzes the CoA-dependent acetylation of GlcN-1-phosphate to GlcNAc-1-phosphate. The LbH domain contains 10 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X. The acetyltransferase active site is located at the interface between two subunits of the active LbH trimer.
Statistics
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PSSM-Id: 100044
View PSSM: cd03353
Aligned: 111 rows
Threshold Bit Score: 246.563
Threshold Setting Gi: 81624390
Created: 16-Aug-2001
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active GlmU is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Comment:The structure evidences show only the interaction of CoA and substrate to one interacting LbH subunit/surface.
  • Structure:2OI7_A; Escherichia coli GlmU binds Desulpho-CoA and GlcNAc-1-PO4; defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:2OI6_A; Escherichia coli GlmU binds CoA and GlcN-1-PO4; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1HV9_A      251 GVMLRDPARFDLRG--------TLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNs-VIGDDCEISpyTVVEDANLA 321 Escherichia coli
2OI7_A      251 GVMLRDPARFDLRG--------TLTHGRDVEIDTNVIIEGNVTLGHRVKIGTGCVIKNs-VIGDDCEISpyTVVEDANLA 321 Escherichia coli
AAS13887    227 RNDLIKAEFYFQENkr----kiFTDSGVTLVAPETVFFSLDTQIARDSVIYPYVFFGTgvKIESGAKILpfSHLENCLIK 302 Wolbachia endosy...
Q5FUY6      226 RSELARAEATLQTRlr----naAMDAGVTLVAPETVFFSTDTVIEADVTIEPNVFFGPgvKVRSGALIRafSHLEGCEVG 301 Gluconobacter ox...
Q5LPQ1      228 RADLAAADAIFQTRar----aeLLDLGVTLMAPETVYLAADTVIGRDTVIEPNVVFGPgvTVESGATIRafSHLEGCHVS 303 Silicibacter pom...
Q5PBV0      221 RADLAIAESYFQCMkr----asFLQSGVTLTSPDQVFFSIDTQIAQDVIVHPYVVFGAgvAVEPGAEILsySHLEFCHIK 296 Anaplasma margin...
Q9A5Z3      239 QAELAAAEAVWQQNrr----kaLMVDGVTMPAPDTVHLAWDTQIAGGAVVEQFVVFGPgvSVASGAVIKafSHLEGAVVG 314 Caulobacter vibr...
Q2W7U1      226 GVNARSELAVAEALvqarlreaAMDNGATLIDPATVWFSWDTKIGRDVTIWPHVVFGPgvTVGDNVEIKgfCHFEGCMVE 305 Magnetospirillum...
Q2RPX0      228 RSELAAAEAVIQGRlr----ekAMEGGATLTAPETVFFSADTRLGRDVSIGPFVTFGPgvEIGDGVEIKgfCHIEGARVA 303 Rhodospirillum r...
ZP_01017942 249 RAELAAAEATFQRRlr----leMMLAGVTLEDPDSTYFAFDTQIAEDVTVAPHVVFGPgvRIAANVRIEafSHIEGAVIE 324 Parvularcula ber...
Feature 1                  #               # #           #  #          ####    ###    #         
1HV9_A      322 AaCTIGPFARLRPGAELLeGAHVGNFVEMKKARLGkGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGaNKFKTIIGDDV 401 Escherichia coli
2OI7_A      322 AaCTIGPFARLRPGAELLeGAHVGNFVEMKKARLGkGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGaNKFKTIIGDDV 401 Escherichia coli
AAS13887    303 SnAEVGPFTRIRGNTTIGnKAKIGNFVEVKTSEVGqNTRIKHLSYIGNAKVGQESNIGAGTIVCNYDGkNKHETNIGSNC 382 Wolbachia endosy...
Q5FUY6      302 EnAMIGPYARLRPGTLCAaQTHVGNFVELKNVELGeGAKANHLTYLGDASIGSGTNVGAGTITCNYDGvFKHRTTIGERV 381 Gluconobacter ox...
Q5LPQ1      304 RgAVVGPYARLRPGAELAeNARIGNFVEIKNAEIGeGAKVNHLTYIGDASIGAGSNIGAGTITCNYDGvMKHRTTIGANV 383 Silicibacter pom...
Q5PBV0      297 KgAIVGPFARVRGNSTIDrGCVVGNFVEIKESSLGeMSKVKHLSYLGNSTIGKNTNVGAGTVICNYDGrNKQHSDIGNNC 376 Anaplasma margin...
Q9A5Z3      315 EgALIGPYARLRPGAEIGpDAHIGNFVEVKKVKVGaGAKANHLSYLGDGSVGEKANIGAGTIFCNYDGfEKFETHVGKGA 394 Caulobacter vibr...
Q2W7U1      306 AgVAAGPFTRLRPGAEIGeGAHIGNFVEVKKATVEaGAKINHLAYVGDARVGAGANVGAGTITCNYDGfNKSFTDIGAGA 385 Magnetospirillum...
Q2RPX0      304 AkATLGPYARLRPGATIAeGAHVGNFVEIKNSAVEqGAKVNHLTYIGDARVGARANIGAGTITCNYDGfGKYHTDIGEGA 383 Rhodospirillum r...
ZP_01017942 325 EgAVVGPFARLRPGTSVGaAARVGNFVETKNTSLGrGAKASHLTYLGDAEIGAGSNIGAGTITCNYDGyEKHRTLIGENC 404 Parvularcula ber...
Feature 1       # ##                ##                #   
1HV9_A      402 FVGSDTQLVAPVTVGKgATIAAGTTVTRNVGEnALAISRVPQ 443 Escherichia coli
2OI7_A      402 FVGSDTQLVAPVTVGKgATIAAGTTVTRNVGEnALAISRVPQ 443 Escherichia coli
AAS13887    383 FVGANSSLIAPLNIHDeSVIAAGSVIVEDVPEkSLAIAREKQ 424 Wolbachia endosymbiont of Drosophila melanogaster
Q5FUY6      382 FVGSDSILVAPVTVGDdALIAAGSVITSDVPPgDLALGRARQ 423 Gluconobacter oxydans
Q5LPQ1      384 FIGSNTMLVAPVTLGDgAMTATGTVVTRDVEPdALAKARVKQ 425 Silicibacter pomeroyi
Q5PBV0      377 FVGANSTIVSPIKVGDnAAIAAGSVITEDLPPrSLGIARSRQ 418 Anaplasma marginale str. St. Maries
Q9A5Z3      395 FIGSNSALVAPVRVGDgAMTGSGSVITKDVEDgALALSRADQ 436 Caulobacter vibrioides
Q2W7U1      386 FIGSNTSLVAPVKVGDgAVVGAGSVITKEVTPgALAVARGSQ 427 Magnetospirillum magneticum AMB-1
Q2RPX0      384 FIGSNTALVAPVSIGAgAIIGAGSTIARDVEAdALALTRGPH 425 Rhodospirillum rubrum ATCC 11170
ZP_01017942 405 FVGSNSSLVAPVTIGAgAYIGSGSVITKTVEPdSLAVARGRQ 446 Parvularcula bermudensis HTCC2503

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