2IU8


Conserved Protein Domain Family
LbH_LpxD

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cd03352: LbH_LpxD 
Click on image for an interactive view with Cn3D
UDP-3-O-acyl-glucosamine N-acyltransferase (LpxD): The enzyme catalyzes the transfer of 3-hydroxymyristic acid or 3-hydroxy-arachidic acid, depending on the organism, from the acyl carrier protein (ACP) to UDP-3-O-acyl-glucosamine to produce UDP-2,3-diacyl-GlcNAc. This constitutes the third step in the lipid A biosynthetic pathway in Gram-negative bacteria. LpxD is a homotrimer, with each subunit consisting of a novel combination of an N-terminal uridine-binding domain, a core lipid-binding left-handed parallel beta helix (LbH) domain, and a C-terminal alpha-helical extension. The LbH domain contains 9 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X).
Statistics
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PSSM-Id: 100043
View PSSM: cd03352
Aligned: 140 rows
Threshold Bit Score: 184.534
Threshold Setting Gi: 85375484
Created: 16-Aug-2001
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active LpxD is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Structure:2IU8_A; Chlamydia trachomatis LpxD binds UDP-GlcNAc and palmitic acid; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
2IU8_A       136 TAIIE---DHVCIEPYAVVC-----QHAHVG---SACHIGSg-SVIGAYSTVGEHSYIHPRVVIRErVSIGKRVIIQPGA 203 Chlamydia trach...
ZP_00951900  116 TATIA---DSARLAPGVIVG-----PDAVIG---ENARIEAg-AIIGPGVVIGDHARIGVRANVQC-ALVGARCEISAGA 182 Oceanicaulis al...
AAS73138      92 IFKHDseiKIPCIHKSATVS-----KDAEVH---KDVYIGPn-VFIGPNCKVHSGVEIHANCSLVRdVTVGSNSIIHHGT 162 uncultured mari...
CAF28700      99 NMIYKk-kQLVGISSRASIS-----PTAKIG---RNCYIGDf-TVIGDNCEIGDDSIVYDRVSLVQnCTLGDACVIQQGV 168 uncultured cren...
YP_756620    121 SAKIG---AGTRLGAGVVIG-----EGAEIG---TDCVIGPh-CVIGPGCRIGDRSRLSPHVSLQC-SDIGADCNILAGA 187 Maricaulis mari...
YP_760482    119 LAQIH---ANAIVQPGAVIG-----PGAAVG---EGAVIGAn-AVIGPGVQIGRNTSIGANASIHC-ALVGDQVTILAGA 185 Hyphomonas nept...
YP_001311773  81 FYLIH---NCLCRDINLYNKe---eFETIIG---DNCIIRDrkGISRKNVIIGNDVTIEENVIIREnVKILDNSVIRSGV 151 Clostridium bei...
YP_928126     71 FVLVDn-pRDLFAQALLVLKsenliSDVHPHfisPKAIISPt-ASIDFGVQIADDVVIEDFVSVKSgTIIGEGTLIRSFT 148 Shewanella amaz...
YP_459546     84 SEIHA---RLAENHAQELRS-----VPNEID---SSARIDPsaHIADHGVTIGPNAWIGPHCAITPgVNVGEGCVLHSGT 152 Erythrobacter l...
YP_001404899  81 YEIHA---SLSRQTGGAFER-----LENTIH---SSSTIHPsaTIASRGVRIGRNVEIGKNVVIHEqTIIDDGVIIRSGS 149 Candidatus Meth...
Feature 1              ###                                                 #   #  ##         ##  
2IU8_A       204 VIGSCGFGYvtsafgqhkHLKHLGKVIIEDDVEIGANTTIDRGR--FKHSVVREGSKIDNLVQIAHQVEVGQHSMIVAQA 281 Chlamydia trach...
ZP_00951900  183 VVGEAGFGLayen-gevfTLPHLGRVIIEDEATLGANATVDRGM--LKDTRIGKGARIDNLCHIAHNVDVGEYAVMAAFA 259 Oceanicaulis al...
AAS73138     163 ILGSEGFGYapsd-dgyvKIEQLGGLSLGKNVEIGANCTIDRGA--LDDTQIHDGVKLDNLVHIAHNVVLGKNSAIAASC 239 uncultured mari...
CAF28700     169 TLGADGFAFerdtsgnleRFPHIMGVKIGKNVEISANSSVARGS--LSDTVIGDGSKLDALVHVAHNVKIGKYCELTAGT 246 uncultured cren...
YP_756620    188 VIGEDGFGIavsn-gntvGILHLGSVLIGDHVTIGANCTIDRGL--FGATRIGASSKIDNLCHIAHNADIGENVIMAGYS 264 Maricaulis mari...
YP_760482    186 RIGETGFGVlvgp-qgaeDSPHFGRVIIQDHVTIGANSCIDRGV--FEDTIIGERTKIDNLCQIAHNVVLGRGVIVAAFG 262 Hyphomonas nept...
YP_001311773 152 ILGGEGFQFnkeg--rifFIEHCGGVEIGRNVEVQYNTCIDKAMfpWDNTVIGEETKIDNLVHVGHGAKIGKRCLIAANA 229 Clostridium bei...
YP_928126    149 CVGSEGFGIaksadgnniRFLHLGGVKIGKYCEIGLFNSINCGT--LSDTIIGDYVKTDAHVHIAHNCTIGNNSILTAAA 226 Shewanella amaz...
YP_459546    153 ALGVPGFNTgiig-grlkIVPQMGGVRLGPHVEMLANCTVARGIf-GGHTSLGEETVADNLVYIAHDVQIGRRVQICALV 230 Erythrobacter l...
YP_001404899 150 VVGNTCTLKgkq---dciDMHPSGGVHIHRDVDIHANTIIDRAVf-KGYTVIGRQTKVDNLVHIGPGVRIGERCLVVACA 225 Candidatus Meth...
Feature 1          ###                ##          #                           
2IU8_A       282 GIAGStkIGNHVIIGGQAGITGHICIADHVIMMAQTGVtkSITSPG-IYGGaPARPYQEIH 341 Chlamydia trachomatis
ZP_00951900  260 GISGStvIGAGAQFGGRVGVADHLKIGQGARLAADAAVm-KDVPAGeTWAGsPAQPIQSFM 319 Oceanicaulis alexandrii HTCC2633
AAS73138     240 AIAGSsvIGENFQMGGLSGVLGHLSICNDVTVGAHTLItkNIEKPGnYVGImPAQKHQDWA 300 uncultured marine gamma proteobact...
CAF28700     247 IIGGSttLGDMVWTGLNSMIKDNIKIGNNVIVAASAGVi-HDVVDGdIVAGvPAKSIRDKV 306 uncultured crenarchaeote
YP_756620    265 GLAGSavIADNAMLGGRVGVYDHVTIGEGARVGANSAAs-RDVPAGeFWVGnPAQPMRQHM 324 Maricaulis maris MCS10
YP_760482    263 GISGSvrVGDGSMLGGRVGIADHVKVGDRVSLAASAGLf-RDVDSGeTWGGtPAKPIRQWM 322 Hyphomonas neptunium ATCC 15444
YP_001311773 230 LIGGSsiIGNDCWIGVSVTISNGLIVGNNVSIKIGAVVt-TNIADGsAVSGnFAIEHSKFI 289 Clostridium beijerinckii NCIMB 8052
YP_928126    227 VLSGGvsIGDNVWLGPNSSIIQKTSIGSDSLIGIGAVVt-KNIDSNvIAAGnPSKILRSNK 286 Shewanella amazonensis SB2B
YP_459546    231 NVLGRtiVGDEAYLGPSCVVKNGLVLGARARVNIGAVVt-TDLAEDaIVSGnFAVPHDRFL 290 Erythrobacter litoralis HTCC2594
YP_001404899 226 NIGDCvvIGNDSWIGPNSTLAEQILIGNQAYITLGSHVs-RDVGDNmLVKDkYVIDRQRFK 285 Candidatus Methanoregula boonei 6A8

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