1J2Z,2AQ9,2QIV,2JF3


Conserved Protein Domain Family
LbH_UDP-GlcNAc_AT

?
cd03351: LbH_UDP-GlcNAc_AT 
Click on image for an interactive view with Cn3D
UDP-N-acetylglucosamine O-acyltransferase (UDP-GlcNAc acyltransferase): Proteins in this family catalyze the transfer of (R)-3-hydroxymyristic acid from its acyl carrier protein thioester to UDP-GlcNAc. It is the first enzyme in the lipid A biosynthetic pathway and is also referred to as LpxA. Lipid A is essential for the growth of Escherichia coli and related bacteria. It is also essential for maintaining the integrity of the outer membrane. UDP-GlcNAc acyltransferase is a homotrimer of left-handed parallel beta helix (LbH) subunits. Each subunit contains an N-terminal LbH region with 9 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X), and a C-terminal alpha-helical region.
Statistics
?
PSSM-Id: 100042
View PSSM: cd03351
Aligned: 119 rows
Threshold Bit Score: 313.215
Threshold Setting Gi: 53802408
Created: 16-Aug-2001
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active UDP-N-acetylglucosamine acyltransferase is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Comment:The structure evidences show only the interaction of substrate to one interacting LbH subunit/surface.
  • Structure:2QIV_X; Escherichia coli UDP-N-acetylglucosamine acyltransferase binds UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc; defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:1J2Z_A; Helicobacter pylori UDP-N-acetylglucosamine acyltransferase binds octyl thioglucoside; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                         #  #          
1J2Z_A        3 KIAKTAIISPKAEINKGVEIGEFCVIGDGVKLDEGVKLHNNVTLQGHTFVGKNTEIFPFAVLGTQPQDLKYKGey-seLI 81  Helicobacter pylori
2QIV_X        7 FVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVLKSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGep-trVE 85  Escherichia coli
NP_848955     9 TIHPTALVHPGAQIGKNVSIGAYSVVGAYVWIGDDTKIGSHVMIDGKTYIGKANQIMCFCAIGVVPQDLKYKHeg--mTY 86  Cyanidioschyzon ...
ZP_01121216   1 MNQPLAYVHPGAKIAKNVVIEPFATIHNNVTIGEGSWIGSNVTIMEGARIGKNCNIFPGAVISAPPQDLKYQGee-ttVE 79  Robiginitalea bi...
CAH06570      1 MISPLAYIHPEAKIGENVEIAPFVYIDRNVVIGDNNKIMANANILYGSRIGNGNTIFPGAVIGAIPQDLKFKGee-stAE 79  Bacteroides frag...
YP_513308     1 MIHSLAVVHESAKIADSAIIGPFCVIGKNVVIGENTELKSHVTIGDNAVIGKNNRIFQYASIGDDPIDYTYKKgdfsqVV 80  Francisella tula...
NP_904420     6 KISPLAWVDPHAEIGVGVEIGPFAVVEAGAKIGDGSILHPHAVVRYGSTLGKGCEIHPNAVIGGVPQDLKFQGed-ttAI 84  Porphyromonas gi...
Q9TLX4        3 NIHSTAIVHPAASLGRNVVVGPYSIIGSDVSIGDYTRIGPHVVITGKTVIGCNNQILSGCILGSVPQDLRYIDseltgLY 82  Cyanidium caldarium
CAH05931      1 MISPLASIAPGAKIGKNVIIQPFAYIEDNVEIGDDCIIMPYASVLNGTRLGKGNKVYQHAVLGAEPQDFHYKGee-ssLI 79  Bacteroides frag...
ZP_02033553   1 MISPLAYVDASAKLGANVTVHPFAYIDKNVEIGDDCEIMPHVSIMSGTRMGKRNRIFNGAVIAAEPQDFNYKGdd-tiVE 79  Parabacteroides ...
Feature 1                    #                      #  #                  #               ##    
1J2Z_A       82 IGEDNLIREFCMINPGTEggikkTLIGDKNLLMAYVHVAHDCVIGSHCILANGVTLAGHIEIGDYVNIGGLTAIHQFVRI 161 Helicobacter pylori
2QIV_X       86 IGDRNRIRESVTIHRGTVqggglTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVHQFCII 165 Escherichia coli
NP_848955    87 IGNGNFIREYVSIHRATKg---vTYIGNENLLMAYTHIAHDCQISDHVILVNGVNLGGHVRIGRYAVIGGLTGLHQFVEI 163 Cyanidioschyzon ...
ZP_01121216  80 IGNNVTIRECATINKGTSdr-mkTVIGKNCLIMAYCHIAHDCVVGNNCIFSNNSTLAGHVTIGDYVVLAGLVAVHQFVSI 158 Robiginitalea bi...
CAH06570     80 IGDNNLIRENVTINRGTAak-grTIVGNNNLLMEGVHVAHDALIGNGCIVGNSTKMAGEIIIDDNAIISANVLMHQFCRV 158 Bacteroides frag...
YP_513308    81 IGDNNIIRECATIHGGTAkeigvTSVGNNNIIMCYVHIGHDCKMGSYINLVNGVGLAGHVHIDDYAILSSNVGVHQFCRV 160 Francisella tula...
NP_904420    85 LGDYTIVRECATVNRGTAsr-gtTVVGSHCLLMAYSHIAHDCVLGDHIIVGNASQIAGEVEIDDHAIISGGVLIHQFVRI 163 Porphyromonas gi...
Q9TLX4       83 IGNNNLIRENVTVHRASGng--vTYIGNNNLIMVNCHVAHDCQIRNNIIISNSVSLAGHVIIDSCVIIGGHAGLHQFVHV 160 Cyanidium caldarium
CAH05931     80 IGDNNHIRENVVISRATFgg-naTKIGNGNFLMDKVHICHDVQIGDNCVAGIGTTIAGECTLDDCVILSGNVTLHQYCHV 158 Bacteroides frag...
ZP_02033553  80 IGDDNVIRENVVINRATNsg-gkTVIGNGNFLHEGVHVSHDTHIGNHSVFGYGSKISGNCMIEDYVIFGGNVLVSQGCRV 158 Parabacteroides ...
Feature 1              #                          #     ##                                      
1J2Z_A      162 AKGCMIAGKSALGKDVPPYCTVEGnra--firGLNRHRMRQLles----kdIDFIYALYKRLFrp-------ipSLRESA 228 Helicobacter pylori
2QIV_X      166 GAHVMVGGCSGVAQDVPPYVIAQGnha--tpfGVNIEGLKRRgfs---reaITAIRNAYKLIYrs-------gkTLDEVK 233 Escherichia coli
NP_848955   164 GRLSMIAGMSRIDRDVPPYMIAEGnpa--rlrGINLVGLTRRk-------vEEAHKSALKKMWmk--------mKKREGI 226 Cyanidioschyzon ...
ZP_01121216 159 GTHAFVTGGSLVRKDVPPYVKGARepm--syvGINSVGLRRRgft---aekIREIQNIYRILYqr-------nyNNSQAV 226 Robiginitalea bi...
CAH06570    159 GGYVMIQGGCRFSKDIPPYIIAGRepi--aysGINIIGLRRRgfs---neiIENIHNAYRIIYqs-------glNTSDAL 226 Bacteroides frag...
YP_513308   161 GKHAFIAHAALVGKDVPPYLMVTAvnagstpcGINTEGLKRRgft---peeMKKIKEVYKVLYrk-------glMMKEAF 230 Francisella tula...
NP_904420   164 SQHVMIQGGSRLSKDIPPYVLVGRdpl--vycGINIVGLRRRnft---neqIFLINDIYRTLYqr-------glNNSDAI 231 Porphyromonas gi...
Q9TLX4      161 GALSMIAAMSKIEKNVLPFVVVSGmpa--itrTINLVGLKRYgisktdinyIRLMLENLKVQPlafdtysifkkFKSDNL 238 Cyanidium caldarium
CAH05931    159 GQWTLVQSGCRISKDVPPYSIMAGnpv--eyhGVNAVVLQQHknts--ervLRHIANAYRLIYqg-------nfSLQDAV 227 Bacteroides frag...
ZP_02033553 159 GTWSMTQTGCRFRKDVPPYIVAALept--tyyGVNSVILMHEgms---ekiVKHISHAYRIIYqg-------ntSIFDAL 226 Parabacteroides ...
Feature 1                                    
1J2Z_A      229 KLEleehannpfVKEICSFIl--eSSRGV 255 Helicobacter pylori
2QIV_X      234 PEIaelaetypeVKAFTDFFa--rSTRGL 260 Escherichia coli
NP_848955   227 EGIekin--ddyVRQVQRFMe--kSERGV 251 Cyanidioschyzon merolae strain 10D
ZP_01121216 227 QIIeaeveatpeRDEILQFIr--dSQRGI 253 Robiginitalea biformata HTCC2501
CAH06570    227 TKVeaevpaspeIEYIVDFIr--nSERGI 253 Bacteroides fragilis NCTC 9343
YP_513308   231 EIIkamakedkvIEPFVDVIg--tSRRGI 257 Francisella tularensis subsp. holarctica
NP_904420   232 DIIqqeyadcheKELILDFIk--sSKRGI 258 Porphyromonas gingivalis W83
Q9TLX4      239 LKRnv------aVQYFSDFLfnsfRARGF 261 Cyanidium caldarium
CAH05931    228 QKIidqvpmseeIENIVAFVk--eSKRGI 254 Bacteroides fragilis NCTC 9343
ZP_02033553 227 LMIkdqvpmsdeIQHIIDFIs--aSKLGI 253 Parabacteroides merdae ATCC 43184

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap