1MR9,1XAT,1KHR


Conserved Protein Domain Family
LbH_XAT

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cd03349: LbH_XAT 
Click on image for an interactive view with Cn3D
Xenobiotic acyltransferase (XAT): The XAT class of hexapeptide acyltransferases is composed of a large number of microbial enzymes that catalyze the CoA-dependent acetylation of a variety of hydroxyl-bearing acceptors such as chloramphenicol and streptogramin, among others. Members of this class of enzymes include Enterococcus faecium streptogramin A acetyltransferase and Pseudomonas aeruginosa chloramphenicol acetyltransferase. They contain repeated copies of a six-residue hexapeptide repeat sequence motif (X-[STAV]-X-[LIV]-[GAED]-X) and adopt a left-handed parallel beta helix (LbH) structure. The active enzyme is a trimer with CoA and substrate binding sites at the interface of two separate LbH subunits. XATs are implicated in inactivating xenobiotics leading to xenobiotic resistance in patients.
Statistics
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PSSM-Id: 100040
View PSSM: cd03349
Aligned: 95 rows
Threshold Bit Score: 126.505
Threshold Setting Gi: 68548787
Created: 21-Jul-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active streptogramin acetyltransferase is homotrimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Structure:1KHR_A; Enterococcus faecium streptogramin acetyltransferase Vat(D) binds CoA and substrate Virginiamycin; defined at 3.5A contacts. Shown are two active sites within the trimer.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 # #                                         ## #          ##          
1MR9_Z       29 NVEVGE--YSYYDskngetfdkQILyhypilndkLKIGKFCSIGPGVTIIXngaNHRXd---gSTYPFNLfgngwek--- 100 Enterococcus fae...
1KHR_A       29 NVEVGE--YSYYDskngetfdkQILyhypilndkLKIGKFCSIGPGVTIIMngaNHRMd---gSTYPFNLfgngwek--- 100 Enterococcus fae...
ZP_01225167 120 SIVMGK--RSYISgp------sFVRgg-----grFLVGSYCNLAENLKIFTgsdAHPLnh--aAMMNFHGnsrlvedg-l 183 marine gamma pro...
YP_587854    66 PAFIGG--FTYVNv-------gAVVfp------hVRIGKFCSIGRNVQIGAa--RHPVef--lSSHPFQFspalfnrvpg 126 Ralstonia metall...
CAM74561     80 DSFFGLgsALYIGr-------hSHMlgngmispmLTIGRHCSISQNVVLGGg--RHPMeylttGHIPGAGeersyya--- 147 Magnetospirillum...
ZP_01753389  16 KIEIGR--FTHGYetv----nfRNWgqg----anVQLGSFCYFSENIQVILd-pQSPFdr--iAGFPFGKvfqeelg--- 79  Roseobacter sp. ...
ZP_00972469  54 ALEVGA--YTDVVs-------gCEFle------vASIGRFCSIATGVVIGQprrSHPMhw--lSTHAFTAnpkll----- 111 Pseudomonas aeru...
ZP_00588256  37 KTFYMAp-GSHVSk-------dLIAgk------ySFIGSECMIGPKVKLGNyvmFAPRv---aVVGADHIfnksgr---- 95  Pelodictyon phae...
ZP_01521515  35 VSWFGF--ASYGNn-------iTVRs-------yVEVGRYCSIGRDVILGLg--HHDYtn--iSTSPYFEkvgfnn---- 90  Comamonas testos...
EDO54425     45 NSIIGD--YSYVNy-------nSIIh-------cCHIGKFCSIGPNVVAGLg--NHPVek-nvTTSPRLFlkgkf----- 100 Bacteroides unif...
Feature 1                             #  #                ##    #       # ## #   #              
1MR9_Z      101 -hxpkldQLPIKg-DTIIGNDVWIGKdVVIXPGVKIGDGAIVAANSVVVKDIAPYXLAGGNPANEIKQRFdqdtiNQLLD 178 Enterococcus fae...
1KHR_A      101 -hmpkldQLPIKg-DTIIGNDVWIGKdVVIMPGVKIGDGAIVAANSVVVKDIAPYMLAGGNPANEIKQRFdqdtiNQLLD 178 Enterococcus fae...
ZP_01225167 184 smnltykELPDStsCVEMGNDVWIGRdVTLKNGVNVGDGCVIGQHSLVLNDCLPYGVYVGSPARLIRFRFpehtiQDLCS 263 marine gamma pro...
YP_587854   127 ypalqteKWRFHa-PTTVGNDVWLGTnCVIVAGVKIGDGAVVGAGAVVTKDVAPYEIVGGVPARRIRLRFdedkvSRLLK 205 Ralstonia metall...
CAM74561    148 daedhfaAGDTSa-FTRIGCDVWIGAnAMVLRGRTIGTGACIGGGAVVTHDVPPYAVVVGNPAKVIRFRFpdkiiESLLR 226 Magnetospirillum...
ZP_01753389  80 -gedlpiQKDCEw-DVSIGNDVRIGDgVTIMSGVTIGNGAVIAANSTVFNDVGAYEIWGGNPATFLQKRFddeliSALEE 157 Roseobacter sp. ...
ZP_00972469 112 --rkplqPEREAt-PARIGHDVWIGRdALILDGVEIGTGAVIGAQSLVNRDVPPYAVVAGSPARVLRYRFapeliERLLA 188 Pseudomonas aeru...
ZP_00588256  96 --piifsGRPEMp-LTIIEDDAWVGYgSILMAGIRIGRGAIVAAGAVVTKDVPAYEIHGGVPAKKIGIRFqt-ieDCQFH 171 Pelodictyon phae...
ZP_01521515  91 -nkiklaCENPKr-RVIIGNDVWIGDkAMVVSGVSVGDGCVIAAGSVVTKDVPPYSIVAGVPAKVIKNRFpdeviSELID 168 Comamonas testos...
EDO54425    101 ---lledRYDQFa-IVTIGNDVWIGAnVTIVNGVTIGDGAVIGANSIVTKDIPPYSIYGGVPAKCIRMRFeqnqiDFLLK 176 Bacteroides unif...
Feature 1              
1MR9_Z      179 IKWWNWP 185 Enterococcus faecium
1KHR_A      179 IKWWNWP 185 Enterococcus faecium
ZP_01225167 264 LKWWDWP 270 marine gamma proteobacterium HTCC2207
YP_587854   206 TRWWDLP 212 Ralstonia metallidurans CH34
CAM74561    227 TRWWTLP 233 Magnetospirillum gryphiswaldense MSR-1
ZP_01753389 158 LAWWNLP 164 Roseobacter sp. SK209-2-6
ZP_00972469 189 SRWWELP 195 Pseudomonas aeruginosa 2192
ZP_00588256 172 DKMLALP 178 Pelodictyon phaeoclathratiforme BU-1
ZP_01521515 169 IKYWAYD 175 Comamonas testosteroni KF-1
EDO54425    177 LKWWNMD 183 Bacteroides uniformis ATCC 8492

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