3BLE,1NVM,1RQE,1S3H,1SR9,1YDN,1YDO,2CW6,2FTP,2NX9,2QF7,3BG3,3BG5,3BG9,3DXI,3EEG,3EWB,3FIG,3HB9,3HBL,3HO8


Conserved Protein Domain Family
DRE_TIM_metallolyase

?
cd03174: DRE_TIM_metallolyase 
Click on image for an interactive view with Cn3D
DRE-TIM metallolyase superfamily
The DRE-TIM metallolyase superfamily includes 2-isopropylmalate synthase (IPMS), alpha-isopropylmalate synthase (LeuA), 3-hydroxy-3-methylglutaryl-CoA lyase, homocitrate synthase, citramalate synthase, 4-hydroxy-2-oxovalerate aldolase, re-citrate synthase, transcarboxylase 5S, pyruvate carboxylase, AksA, and FrbC. These members all share a conserved triose-phosphate isomerase (TIM) barrel domain consisting of a core beta(8)-alpha(8) motif with the eight parallel beta strands forming an enclosed barrel surrounded by eight alpha helices. The domain has a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. In addition, the catalytic site includes three invariant residues - an aspartate (D), an arginine (R), and a glutamate (E) - which is the basis for the domain name "DRE-TIM".
Statistics
?
PSSM-Id: 163674
View PSSM: cd03174
Aligned: 983 rows
Threshold Bit Score: 100.993
Threshold Setting Gi: 225564056
Created: 9-Sep-2009
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1RQE_A; Propionibacterium freudenreichii transcarboxylase 5S binds Co2+ and oxaloacetate, contacts at 4A.
    View structure with Cn3D
  • Comment:The active site contains a carbamylated lysine.
  • Structure:3BLE_A; Leptospira interrogans citramalate synthase binds Zn2+ and malonate, contacts at 4A.
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1            ##  #                            #                                        #
3BLE_A       23 LDVTLRDGEQTRgvsf-stsEKLNIAKFLLQklnvDRVEIASArvs--------kgELETVQKIMEwaateqlteRIEIL 93  Leptospira inter...
1RQE_A       28 TELVLRDAHQSLmatrmameDMVGACADIDAag-yWSVECWGGatydscirflnedPWERLRTFRKlmp----nsRLQML 102 Propionibacteriu...
NP_879691     8 IDTTLRDAQQCLwatrmtagMMDPIAQALDEag-fDMIDFMAPvqfdvcvrylredPFEKARHFRKrfr----ntPMRSY 82  Bordetella pertu...
YP_160306     7 VDVSLRDGNQSLwgavgvktRTIERVCPAIDrvgyRALELLSStqvavavrfhredPWARIDAASRaap----rtPLGFL 82  Azoarcus sp. EbN1
ZP_01034173   8 VDVTLRDAHQCLwatrmttaMMQDIAPRLNRag-fEAIDLVGGavfdvcvrylredPWERMRILNQwit----etPLIIH 82  Roseovarius sp. 217
YP_844811    29 MTNNERDVSQSDfkarimpaTTLRVAPYRDEtg-yFAFEITGGatvhvdlmrkqinPVEKLRLVRKamp----ntLLQAV 103 Syntrophobacter ...
ZP_01904236   8 IDVTLRDAHQCLwatrmttgMMKDIAPFLDRag-fEAIDLVGGavfdvcvrflredPWERMRILNTwit----etPLIIH 82  Roseobacter sp. ...
ZP_01924445  24 MMTAFRDGFQSVfgarvfsqDFLPVVEEAAAag-iTHFEAGGGalfqaayfysnedAFQVMDNFRKiag---psaNLQTL 99  Victivallis vade...
ZP_03933203  34 IDTTLRDAHQSIwatrmttsHILSLLDDITNag-fEHVDLVAPiqfdvsvrylkedPWERVRLVHEnaa---pgtKFRAL 109 Corynebacterium ...
ZP_04495938   8 IDVTLRDGNQSLwsatgidtETVLAVAPLLDqvgfKALDFTTSthmaisvryhqedPFERIRLTSQlmp----ntPLIFM 83  Sphaerobacter th...
Feature 1        #                                 #                                          # 
3BLE_A       94 GFvd-------------gNKTVDWIKdsgakVLNLLTKgslhhlekqlgktpkeffTDVSFVIEYAIKSg-lKINVYLEd 159 Leptospira inter...
1RQE_A      103 LRgqnllgyrhyndevvdRFVDKSAEng-mdVFRVFDAmn--------------dpRNMAHAMAAVKKAg-kHAQGTICy 166 Propionibacteriu...
NP_879691    83 CRskslvgfsmvpddmveLWIDCLAAng-fsAVGTLDSll--------------dvDNMKVSVRRAKKIg-iKSIGALVf 146 Bordetella pertu...
YP_160306    83 TTgkrfisfsrtpanlfqLAFDLLRRhg-vtRMWIIDPmh--------------dmASARETARMARRAgfdEVVAGICy 147 Azoarcus sp. EbN1
ZP_01034173  83 TRgqslftfeffpddiveLAAERFAAng-mrYHTPYDAln--------------dmRNLEIPVRAAKQHg-lHVVGGLVy 146 Roseovarius sp. 217
YP_844811   104 CRgrnlfgyrpypdnvvrMTVRLFSEyi--dVWRLYDFln--------------hiPNLMVAAEEVKKAg-kLLMPCICf 166 Syntrophobacter ...
ZP_01904236  83 TRgqslftfeffpddiveLAAERFAAng-mrYHTPYDPln--------------diRNLRIPVQAARKLg-iHTVPGLVy 146 Roseobacter sp. ...
ZP_01924445 100 ARginvvgldsqprdiikLHAQLMHKhg-isTIRNFDAln--------------dvNNLIYSGKCIVEAg-lKHEVTVTm 163 Victivallis vade...
ZP_03933203 110 IRsknlasfdflpddailNWTERLYAng-frVIGSFDGln--------------diDNIDQGLKLAKELg-aETFGALSy 173 Corynebacterium ...
ZP_04495938  84 TTgmrfitwepapraiirLAMQLVIKhg-irRIQIVEPmn--------------nmPALLEAAKIAREEgaeQVVAGLVy 148 Sphaerobacter th...
Feature 1                                         # #                            # #            
3BLE_A      160 wsng---frnspDYVKSLVEHLSkeh--ieRIFLPDTLGVLSPEETFQGVDSLIQKypd-ihFEFHGHNDYDLSVANSLQ 233 Leptospira inter...
1RQE_A      167 tisp----vhtvEGYVKLAGQLLdmg--adSIALKDMAALLKPQPAYDIIKAIKDTygqktqINLHCHSTTGVTEVSLMK 240 Propionibacteriu...
NP_879691   147 cesp----lhtdALYAGTAAELVrcg--vdGIMLKDSGALLTIERIRTLIPALRAAvgd-ipIEVHSHSNTGLVQRVYLD 219 Bordetella pertu...
YP_160306   148 tisp----vhtdDYFADRVAELDdce-aidSVYLKDPAGLLTPARLESLVPHMRQRlkrlriDELHTHCNTGLAPITLLT 222 Azoarcus sp. EbN1
ZP_01034173 147 tfsp----vhtdAYYQKKAKELMalg--vdGIFLKDASGLLRPERIATLVPALKTVmgn-mpLQIHTHCNSGLAPYVVLQ 219 Roseovarius sp. 217
YP_844811   167 stgp----ehtdAFYAGKVAEILsamgediILCIKNHSSLGSALRIGELVKAIRREfpn-llLAYHGHNTDGNDLARMVA 241 Syntrophobacter ...
ZP_01904236 147 tysp----vhtdEYYRKKALELVeig--vdGIFIKDPSGLLTPERVATLVPAIKSAigd-lpLQLHSHCLSGLSPYVALQ 219 Roseobacter sp. ...
ZP_01924445 164 melppgcaghtvEFYEGVLRKILdsgipydSVCFKDASGTSTPRKVHETIKMARRLlgkdirIVFHSHETAGCSVLAYMS 243 Victivallis vade...
ZP_03933203 174 clsp----vhtdDLYKRKATELLert-dvdRIMLKDAGGLLTPDRLKTLIPAIRSSigd-vpLEVHTHSLTGLSPLTYLY 247 Corynebacterium ...
ZP_04495938 149 sisp----vhtdEFYVGCAAALAgatdlidTVYIKDPSGLLTPERVETIVPAMRKAlgs-mpLEIHSHCNAGMAPLCYLR 223 Sphaerobacter th...
Feature 1                            #                                  
3BLE_A      234 AIRAGvKGLHASInglgeraGNTPLEALVTTIHdksnskTNINEIAITEAsRLVEV 289 Leptospira interrogans
1RQE_A      241 AIEAGvDVVDTAIssmslgpGHNPTESVAEMLEgtg-ytTNLDYDRLHKIrDHFKA 295 Propionibacterium freudenreichii
NP_879691   220 AADLGiDQLHTSIaplaggvAQPSIQNMTRNLAqrg-yrTDVDMARVEEIsAYLHR 274 Bordetella pertussis Tohama I
YP_160306   223 AADLGiGTLHCALpplangsSHAPAAQLVANLRarg-hrVDVDLDAMEEAsAILRR 277 Azoarcus sp. EbN1
ZP_01034173 220 AVEHGvDVVHTATstlangvSHSPTETVTRNLRrrg-feVDIDLGPIEEVaERLAF 274 Roseovarius sp. 217
YP_844811   242 AVQEGvKIVEVSDhgfggiySQAPALSFIQTLHdygcsaPGLNIQPLLETsDILRR 297 Syntrophobacter fumaroxidans MPOB
ZP_01904236 220 AVEHGiDVLHTATstlahgaSHPPTERVVENLRrrg-hsAALDLDAIREVaERLDY 274 Roseobacter sp. AzwK-3b
ZP_01924445 244 ALEAGaDQLDLSMvpmsggtCQPDLLTMWHALRgte-ydLGIDPRKIMKVeESLKN 298 Victivallis vadensis ATCC BAA-548
ZP_03933203 248 AAELGvDSIHTSIaplangpGQPATQAVVRDLRalg-ytVDVDDERIDSAsERIQE 302 Corynebacterium accolens ATCC 49725
ZP_04495938 224 AAELGvETVHTAIpplaegtSLPSITRTVANLRelg-fdVDIDLEPIERIsQHLFE 278 Sphaerobacter thermophilus DSM 20745

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap