Conserved Protein Domain Family
GAT1_cyanophycinase

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cd03145: GAT1_cyanophycinase 
Type 1 glutamine amidotransferase (GATase1)-like domain found in cyanophycinase
Type 1 glutamine amidotransferase (GATase1)-like domain found in cyanophycinase. This group contains proteins similar to the extracellular cyanophycinases from Pseudomonas anguilliseptica BI (CphE) and Synechocystis sp. PCC 6803 CphB. Cyanophycinases are intracellular exopeptidases which hydrolyze the polymer cyanophycin (multi L-arginyl-poly-L-aspartic acid) to the dipeptide beta-Asp-Arg. Cyanophycinase is believed to be a serine-type exopeptidase having a Ser-His-Glu catalytic triad which differs from the Cys-His-Glu catalytic triad typical of GATase1 domains by having a Ser in place of the reactive Cys at the nucleophile elbow.
Statistics
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PSSM-Id: 153239
Aligned: 35 rows
Threshold Bit Score: 111.61
Created: 2-Nov-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active siteproposed
Feature 1:active site nucleophile [active site]
Evidence:
  • Comment:by similarity to Salmonella typhimurium peptidase E
  • Comment:Active site serine is located in a strand helix motif.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
AAL40891     25 PLILVGGgl----------kdDNTAIYQRLIqlagg-----ngqaRIGVITAAsipesddpdagtadaanskANGEFYAQ 89  Pseudomonas angu...
YP_075551    66 PVILMGGt------------pVPDEAVVAAVhlsg------grsaRLAVVPAAagg-------------daaEAAAVAAR 114 Symbiobacterium ...
BAB74205     24 KLLFQGNtvev-------lpaLAGPVLNLGGggpdv-----eaaiQWMIHQVRgtakvn-------vvvlraYGGNDYNH 84  Nostoc sp. PCC 7120
ZP_00691141  62 SYVLMGGg------------pDVDQAFQWLIqragik---pgtggRFVIIRASg-----------------tEAYNPYIY 109 Rhodoferax ferri...
ZP_00524710  22 YEYYLTGnaadv-----kpatRPGFVLMGGGkdvd-------aafRWMLAKAGggdvv----------vlraSGSDGYNK 79  Solibacter usita...
ZP_00507218  59 MTVLMGGg------------lDVDQAFKDMIakagg----satnkIDVVVIRA-------------------SGTDGYNP 103 Polaromonas sp. ...
YP_143432    17 YFALLTAd-------------PLRGALNRVEarlie----eargeALFLIPYAl-----------------rDLAEAWRL 62  Thermus thermoph...
BAC88058     23 VVMVAGGgsegdigdtaswsyRLYPTLIEAGdvn--------gdgRVTVAVIAd-----------------sSQSEFIPE 77  Gloeobacter viol...
NP_824488    28 HTYLIGGgwd---------erGAEAVYGPFLeaagqragrqraagDAAVVGCLlvde-----------gdgaGQFARYEA 87  Streptomyces ave...
YP_056776     2 SLFLVGGgy----------ddSLVEAYDKFVsqarhh--nadapiAVVVAGPPses--------------adHAAELTRI 55  Propionibacteriu...
Feature 1                                                                                       
AAL40891     90 LLETY--------------------GAADAQWIpidldqisnnsnpQVVAQINSMTGFFFGGGDQSRLTQTLQTatrads 149 Pseudomonas angu...
YP_075551   115 AFTRF--------------------GMKHVEPVlldsre--kaadpEWVARLREYDAIVLCGDSPARGLHVLQAt----- 167 Symbiobacterium ...
BAB74205     85 LIYRMs-------------------GVNSVQTLvisnrq--danrdDIVQKVLNAGVVFFAGGDQCQYIRSWKNt----- 138 Nostoc sp. PCC 7120
ZP_00691141 110 YSDANggtstlppvnqdgwvgggylGLSSVETLvipsvk--aandpTVNQIVARADVVFIAGGDQSDYIKYWKNt----- 182 Rhodoferax ferri...
ZP_00524710  80 YIHEM--------------------GADSVESIvvktae--aardaFVLEKIRHAEALWIAGGDQWNYVRVWGAs----- 132 Solibacter usita...
ZP_00507218 104 YLSAMe-------------------GVDSVETLviktpn--gandpKVTSVILGADVLFIAGGDQWNYISQWNEt----- 157 Polaromonas sp. ...
YP_143432    63 VYRDL--------------------GLRRGRVVylrrre--eafdpEVARRVAESPLVILAAESLPEFLDLIRNs----- 115 Thermus thermoph...
BAC88058     78 YFEWL--------------------GADDAFNVlvatrt--qandpAAVDRVRDVDAIFIKGGDQGKYYDNWNDt----- 130 Gloeobacter viol...
NP_824488    88 VLRKVa------------------dCAPVPLLVplg--------erFDVGALGRVDALLVCGGLTPAYQDALAE------ 135 Streptomyces ave...
YP_056776    56 VASRWvdaniltiyl-dgpgtapmmGAPATPLWaed-------esfQLPDNLDALAGIIVGGGAVPSYINGLAP------ 121 Propionibacteriu...
Feature 1                            #                                                          
AAL40891    150 pALAAIRARHn--aGAVLAGTSAGTAIMvqgpmvtgge-------sydglrygvyTTPSGDDLsy-----dmQGGFGFFn 215 Pseudomonas angu...
YP_075551   168 aAAAALRDHVk--aGRLLVGIDAGAAILgs------------------------rLFPHEAEDg-------iTVGLGIVp 214 Symbiobacterium ...
BAB74205    139 kLEKAVASVYr--rGGAVGGTSAGAMIMsdfiy-----------------dacacEDPIETKDaledpyqniTFTYNFFq 199 Nostoc sp. PCC 7120
ZP_00691141 183 qLDWTLQALMn--kKVPIGGTSAGLAVLgqfdfaalrgtvtsdqalsnpfnkymsLDPTPQTLdpt---sptSIAGGFIt 257 Rhodoferax ferri...
ZP_00524710 133 pMREEIHALId--rGVPVGGTSAGLAVLgqfvftaek---------dsvtsaqalANPYDERV---------KIGEDFVk 192 Solibacter usita...
ZP_00507218 158 sVETIIKTVLlp-kNVPIGGTSAGLAVLgavdfsaqh---------gtitssdalSDPYNRRL---------TLDRTFLe 218 Polaromonas sp. ...
YP_143432   116 lLLQALAEVYrqggGVVAVGEAAGLLGEa-------------------------aFYALEGEVr-------aGFGLALLr 163 Thermus thermoph...
BAC88058    131 rLEDHIRYVVqt-lGGGVGGTSAGAMSQsqyalagekdli----sqdvltnaqtrYLDDADGGsg-----ihADFLGFVa 200 Gloeobacter viol...
NP_824488   136 -VLGRLPRVLae-rGIPYAGFSAGAAVAarravvggwla-----ggvpvcpedtgEDLDEIEV---------REGLGLV- 198 Streptomyces ave...
YP_056776   122 -AAEQLSMLVr--gGAPWLGFSAGAMAPcvtalaggwk-------lqerqvgqqtGAEGFDEVt-------fVEGLGLV- 183 Propionibacteriu...
Feature 1                                                                                   
AAL40891    216 ------YGLLDTHFSergRQGRIVRLADHtq-------vpFAFGVDEntallvqnnatlgqvemeviGENGVFIFD 278 Pseudomonas anguilli...
YP_075551   215 ------ALLIDPGFSgaqRFSRLVRAMSApea-----aamMGVGLDAqtgvlia------dgeakvlGEARVTVLD 273 Symbiobacterium ther...
BAB74205    200 ws-hlrGTIIDTHFDsrkRMGRIMAFIARqiqdg-vsrsaLGIAISEetsvvvdk-----ygkakvlGRNAAYFVL 268 Nostoc sp. PCC 7120
ZP_00691141 258 ps-plfNTILDAHLDerdRMGRLVTFVSRl----------IGPYYESg-------------------CSGGILVPK 303 Rhodoferax ferriredu...
ZP_00524710 193 ip-alrGVITDTHFVkrdRLGRLLTFLARivedg-kaaeaRAIAVDErtaalvep-----dgsvtieGEGPAYFLR 261 Solibacter usitatus ...
ZP_00507218 219 tipklqNTIVDAHLVsrdRMGRLVTFLARmiqdgmpwsaaRAIGVDEgtavvidgd--iatvcansgGTGAAYFLK 292 Polaromonas sp. JS666
YP_143432   164 g----lALLPRVEERg--RFLALSRLVADnp-------dlVGLGLLEntalrll-------rglgevWAGGVTLVD 219 Thermus thermophilus...
BAC88058    201 ------NTVIDSHFNdrgRLARLLGVMAKvnedfglttglLGIGLGDltglvir------gdraeviGQGSVSFLQ 264 Gloeobacter violaceu...
NP_824488   199 ------PFGVDVHAAqwgTLPRLIAAVADgr-------vpHGVAIDEntlltvd------grtarvsGLGRVHTVR 255 Streptomyces avermit...
YP_056776   184 ------SLTISTHNDtlsGDGLIISAVESgl-------lsSAVAVDEatclrida----stgrtevmGRGLVRWFT 242 Propionibacterium ac...

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