1KWK


Conserved Protein Domain Family
A4_beta-galactosidase_middle_domain

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cd03143: A4_beta-galactosidase_middle_domain 
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A4 beta-galactosidase middle domain: a type 1 glutamine amidotransferase (GATase1)-like domain
A4 beta-galactosidase middle domain: a type 1 glutamine amidotransferase (GATase1)-like domain. This group includes proteins similar to beta-galactosidase from Thermus thermophilus. Beta-Galactosidase hydrolyzes the beta-1,4-D-galactosidic linkage of lactose, as well as those of related chromogens, o-nitrophenyl-beta-D-galactopyranoside (ONP-Gal) and 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). This A4 beta-galactosidase middle domain lacks the catalytic triad of typical GATase1 domains. The reactive Cys residue found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow in typical GATase1 domains is not conserved in this group.
Statistics
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PSSM-Id: 153237
View PSSM: cd03143
Aligned: 88 rows
Threshold Bit Score: 34.6966
Threshold Setting Gi: 48763589
Created: 26-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1KWK_A      400 VALVFd-----------------YEAAWIYEvqpq-----------gaewSYLGLVYLFYSALRRLg-------LDVDVV 444 Thermus thermoph...
NP_147161   245 DAMFFyn---------------sHPVGWAGRg-----------------tIVAERARDYLDAVFAEa-------SETDVL 285 Aeropyrum pernix K1
ZP_00519713 429 VEDLYvwh-------------yrNERYLRNErplarvalvysqqtnrffgRTEDHTLGYYQALIEAr-------IPFEMV 488 Solibacter usita...
ZP_00526674 366 AEPVFrwheeheqylinrqpvagIGVVWSQRntdy-----------ygrdESAELVDAPYRGVTEAlirarlpyLPVNVD 434 Solibacter usita...
EAM76391    364 HAEVYdh--------------lvPAARTALIrpdpla-------vpeddsDRGEEFRGLYEALLERh-------VPFDVV 415 Kineococcus radi...
EAM76397    371 EPLLSrl---------------sITADVALLdatrst--------glphgGENLDGQGAYQALVEAg-------IPFEFV 420 Kineococcus radi...
EAM76512    398 VGVVWs-----------------QPNFDLHGred-------------gpnTVIAPYAGLTRVLVTEn-------IPFTPI 440 Kineococcus radi...
CAC46947    280 VLLVSgep-------------haGERAWRNLl------------------KSDAAVDLVHFTILRPpe-----kQDGTPI 323 Sinorhizobium me...
NP_754382     8 LKVLFi-----------------GESWHIHMihskgy-------dsftssKYEEGATWLLQCLKNSq-------VDVTYM 56  Escherichia coli...
CAF26416    285 VLLISgap-------------ynGERTWRDLl-----------------kGDSNIDLVHFTILRPPn------kADNTPL 328 Bartonella quint...
1KWK_A      445 P--------PGASLr-------gYAFAVVPSLpivr-----------------eeaLEAFREAEg--PVLFGPRSGSKTe 490 Thermus thermoph...
NP_147161   286 Tvm----mlPMVTKlsqaavgegKPVLVSRNLfynlrtvqs------atplavkqgIRSIVASGg--HPMATIFSSQYFe 353 Aeropyrum pernix K1
ZP_00519713 489 Hdql--ldaAHTGQ---------FQVLIFPNIaalst--------------gqcaqIRDFVNRGg--SIVATHQTSLYDe 541 Solibacter usita...
ZP_00526674 435 H--------IDRDAg-------rFSALVLPSIaalsd--------------aqcaaVKRFIANGg--GLVATGATSLYDe 483 Solibacter usita...
EAM76391    416 Paerl-celAGQLKr--------YRLLVLGDLgalpp--------------asadvLEGFTAAGg-rMLLTGSTGFVLDd 471 Kineococcus radi...
EAM76397    421 Cdgl---itAEQLDr--------FRVLVAPDVqrlsd--------------eecrtITEWVGRGg--CLVAAHQTSMLHp 473 Kineococcus radi...
EAM76512    441 H--------VDRIAha------dVDVLVLPDLavmse--------------aqceaVRAFVDAGg--SIIATGATSALDe 490 Kineococcus radi...
CAC46947    324 Nelsl-iafPTRELfvdk--iseFDLIIFDRYqhrgvlp-----------ilyydnIAQYVQNGgalLIAAGPEHAGDDs 389 Sinorhizobium me...
NP_754382    57 PahtvqiafPEDVAql-----eqYDAIVISDIgsntfllqndtfyqlrikpnalelIKEYVNNGgglLMIGGYLSFMGIe 131 Escherichia coli...
CAF26416    329 Sqlsl-vvfPTTKLfvee--innFDLIILDGYqhsavlp-----------liyydyIAQYVQKGgalLIITGPEFTQKNs 394 Bartonella quint...
1KWK_A      491 tfqipkelpp-------------------------------------gplqallplkvVRVESLppgllevaegalgrfp 533 Thermus thermoph...
NP_147161   354 dsraida-------------------------------------------laevyeelERVEDKivgsepirymgilyss 390 Aeropyrum pernix K1
ZP_00519713 542 a--------------------------------------------------------gVRRADFgladlfgasfggqida 565 Solibacter usita...
ZP_00526674 484 w--------------------------------------------------------gDPRPDFaladlfg--------- 498 Solibacter usita...
EAM76391    472 pqlpsagigrra---------------------------------avltgpeatwssvVVPEEGspvpilgsfhtletae 518 Kineococcus radi...
EAM76397    474 dgtpradfglgevlgvhltrparqgvrnnyaaltgrhalhtgftgttriiggtaivgvSAVEDAvvpfrfvpdypdlpme 553 Kineococcus radi...
EAM76512    491 h--------------------------------------------------------gREREDFaladl----------- 503 Kineococcus radi...
CAC46947    390 iaatplaavlpa---------------------------------tptgvmnekaffpRLSEEGkkhpvtrglegaadep 436 Sinorhizobium me...
NP_754382   132 akanykntvla------------------------------------dvlpvtmldgdDRVEKPegviaqpsqpehpvik 175 Escherichia coli...
CAF26416    395 laktplisvlpalpngviiqkpfr----------ptltkegerhpvtrglatptypasQWGQWLrqiaiqninkgta--- 461 Bartonella quint...
1KWK_A      534 lglw-------------------------------------------------------rewveaplkplltfqdgkgAL 558 Thermus thermoph...
NP_147161   391 ethekvywnaphyytseiegmamiafhrhlpyeflsmkdihkagrykvlvaaetdvlsdeeeaaledyverggflvvtHS 470 Aeropyrum pernix K1
ZP_00519713 566 rmqnsylrlenqhpilagledtrriing-------vsrvhtkgaypnppltlipsypdlpmeevfprvpktdipevfvRE 638 Solibacter usita...
ZP_00526674 499 ------------------------------------------------------------------------ahapspDF 506 Solibacter usita...
EAM76391    519 vtahrv---------------------------------------------------lsrapygppekcyghleldtpAA 547 Kineococcus radi...
EAM76397    554 ---------------------------------------------------------------evyareaprdpavvlRE 570 Kineococcus radi...
EAM76512    504 -------------------------------------------------------------------------lgahrTA 510 Kineococcus radi...
CAC46947    437 pawgrwfr----------------------------------------------tvdvdrplgqtvmeaadgkpllvlNR 470 Sinorhizobium me...
NP_754382   176 gfseypffl--------------------------------------------gynraiakenaevvltinndpllvfGN 211 Escherichia coli...
CAF26416    462 -----------------------------------------------------------------lmkgadeqpllllSH 476 Bartonella quint...
1KWK_A      559 YREGr-----------YLYLAAwps-------peLAGRLLSALAAEAGL 589 Thermus thermophilus
NP_147161   471 LGRMyp---------dFTYRHSlamee--tlkvrYEGVFYFGYVFVDLE 508 Aeropyrum pernix K1
ZP_00519713 639 IGKGrvvyfpwdidrtFWEVLSpdh-------gkLLRNAVTWAANEPVP 680 Solibacter usitatus Ellin6076
ZP_00526674 507 GRKGatr------ttaHTYLRLpg---------iGERHPALRGFEETDI 540 Solibacter usitatus Ellin6076
EAM76391    548 VRSErvttl--amtpgRAYRASglsglrdlvadeALRLLAADVEIVTDL 594 Kineococcus radiotolerans SRS30216
EAM76397    571 HPGGg----------rTAYVAFnlge----vfweALQPDHGHLLAETVR 605 Kineococcus radiotolerans SRS30216
EAM76512    511 AQRGsvlpp--tpsieIWDRHSylrle--dqahaATDEAAPEAADETAP 555 Kineococcus radiotolerans SRS30216
CAC46947    471 VGKGrvaml---lsdqGWLWARgfe------gggPHVSLYRRTAHWLMQ 510 Sinorhizobium meliloti
NP_754382   212 YHNGkiacf---msdcSPHWGTqqfm-----swpFYTALWVNILTHIAR 252 Escherichia coli CFT073
CAF26416    477 FGKGrvgml---lsdeSWLWARsfe------gggPYAALYRRISHWLMK 516 Bartonella quintana str. Toulouse
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