1ONS,1PV2,1QVW,1U9C


Conserved Protein Domain Family
GATase1_Hsp31_like

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cd03141: GATase1_Hsp31_like 
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Type 1 glutamine amidotransferase (GATase1)-like domain found in proteins similar to Escherichia coli Hsp31 protein
Type 1 glutamine amidotransferase (GATase1)-like domain found in proteins similar to Escherichia coli Hsp31 protein (EcHsp31). This group includes EcHsp31 and Saccharomyces cerevisiae Ydr533c protein. EcHsp31 has chaperone activity. Ydr533c is upregulated in response to various stress conditions along with the heat shock family. EcHsp31 coordinates a metal ion using a 2-His-1-carboxylate motif present in various ions that use iron as a cofactor such as Carboxypeptidase A. The catalytic triad typical of GATase1 domains is not conserved in this GATase1-like domain. However, in common with a typical GATase1 domain, a reactive Cys residue is found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow. For EcHsp31, this Cys together with a different His and, an Asp (rather than a Glu) residue form a different catalytic triad from the typical GATase1 domain. For Ydr533c a catalytic triad forms from the conserved Cys together with a different His and Glu from that of the typical GATase1domain. Ydr533c protein and EcHsp31 are homodimers.
Statistics
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PSSM-Id: 153235
View PSSM: cd03141
Aligned: 81 rows
Threshold Bit Score: 153.096
Threshold Setting Gi: 78212372
Created: 7-Oct-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
conserved cyspotential
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:conserved cys residue [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1ONS_A       50 ILVIAaderylpt-------dngklfsTGNHp-iETLLPLYHLHAAg--FEFEVATISGLm-----tKFEYWAMp----- 109 Escherichia coli
AAD37457      7 ILFVVsgadhwtl-------adgtahpTGFWa-eEAVAPYRAFTDAg--HEVVVATPGGVv------PTVDRGSl----- 65  Streptomyces gri...
AAF10068     10 ILVVMssaaqipl-------qdgknhnTGYFl-nEFGVPAKRLTDAg--YELVIATPRGSv-----pGMDHNSDh----- 69  Deinococcus radi...
NP_436327    25 VLVVLsdsdhldl-------kdgkvfeTGFYl-nELMQPVKALTEAg--HDITFATPKGTa-----pTLDKSSVd----- 84  Sinorhizobium me...
EAL18422      6 ILIIMsdassiplhktsqcgettadqsSGFFl-mELAKPLSKFLSAg--CEVTFASPLGKt-----pTPDPTSEs----- 72  Cryptococcus neo...
EAL94924      4 ILMVVsaadsltm-------rdgtqhpTGYWa-eELVVSHQTLRDAg--HTVHIATPGGAk-----pTVDEVSLa----- 63  Arthrobacter sp....
ZP_00686125  25 VLVVLsdsdhldl-------kdgkvfaTGFYl-nELMQPVKALLDAg--HQVTFATPEGKa-----pTMDATSAd----- 84  Burkholderia amb...
NP_872418    12 CLLVA----------------------SGAAegvSAQSFLHCFTMAstaFNLQVATPGGK-------AMEFVDVt----- 57  human
YP_381151     4 ILMVAs--------------------nLGVWa-eELQGPWDVLNEKg--HNVTLATEQGIkplphviSMDPDALd----- 55  Synechococcus sp...
CAG08765     12 CLIVAsa------------------spQGVSa-kSFQQCFDHCSSL---FNLQTATPGGK-------PIDFSGVdestsr 62  Tetraodon nigrov...
Feature 1                                                                                       
1ONS_A      110 -hKDEKvmpff--------------eqhksLFRNPKKLADVvaslnadseYAAIFVPGGHGALIGLPESQDVAAALQWAI 174 Escherichia coli
AAD37457     66 -aPDVNggqegadav-------aagleafeELRRPVALEDVdp-----dvYDAVFYPGGHGPMEDLAVDPVSGRLLARVL 132 Streptomyces gri...
AAF10068     70 -dMFFKnkeehneik------afvgdvlsnTTIQTTEAVAAegt----qaYAALFLPGGHAPMADLMADPALGEILRQFH 138 Deinococcus radi...
NP_436327    85 -nMYFGgdeaamqesiamldklkltsdsssPVLSLARVEQIgy-----dhFDAVYVPGGHAPMQDLLVSPELGKLLADFH 158 Sinorhizobium me...
EAL18422     73 -lVAFAlnfyerkrendl-iarmmrengfaSPRTFQSISDEel-----dgFAAVFIPGGHAPLADLGADPELGRILAHFH 145 Cryptococcus neo...
EAL94924     64 -aESAGgqdradgfr-------eylakidaELSAPLVLADVdp-----sgYDAVVMPGGHGPMADLYQDADLGRILAEAD 130 Arthrobacter sp....
ZP_00686125  85 -kMYFNgdekamrdyralldklqitsrahsPVISLARVEQIgy-----ghFDAVYVPGGHAPMQDLLTSPAVGRLLADFH 158 Burkholderia amb...
NP_872418    58 --ESNArwvqd---------------frlkAYASPAKLESIdg-----arYHALLIPSCPGALTDLASSGSLARILQHFH 115 human
YP_381151    56 --TVQNikvnpqsvvd-----rckalwastALDNPIAIKDAkm-----sdYEAIVLIGGPGAPLDMNGNSKLHKLLVDAF 123 Synechococcus sp...
CAG08765     63 wvQDFSv----------------------kTYATPAKLESIdg-----arYQVLLIPDCPGALSDLAHSGSMHRILTHFI 115 Tetraodon nigrov...
Feature 1                #                                                                      
1ONS_A      175 kndRFVISLCHGPAAFLalrh------------------gdnPLNGYSICAFPDAADkqtpeigympghlTWYf------ 230 Escherichia coli
AAD37457    133 asgKPLGVVCHGPAALLaatgp----------------dgrsAFAGYRLTGFTNAEEaqagf----adkaKWLl------ 186 Streptomyces gri...
AAF10068    139 aaaKPTALICHAPTALLaaqadpvafqkg-meageqpsaqdfTYQGYRVTVFANDEEeatekt--feapmLYYp------ 209 Deinococcus radi...
NP_436327   159 akgKTTALACHGPIALLstlpdasafttk-letsgsakaegwIYAGYKMTVISNQEEeiakgl-lnggkmKFYp------ 230 Sinorhizobium me...
EAL18422    146 hegKPTAVICHGPYGLLstkat---------------pegtfLYKGYKITSWSDAEEkmmet---lwggeVPRv------ 201 Cryptococcus neo...
EAL94924    131 ragKVIAPFCHGPAGLLsavdg----------------dgkfAFAGRHLTVFSDDEElsggt----gpntPWFv------ 184 Arthrobacter sp....
ZP_00686125 159 argKTTALVCHGPIALLstlpdapgftrqlaagkagdgapkwIYAGYRMTVISNAEEeqakal-lgggamKFYp------ 231 Burkholderia amb...
NP_872418   116 sesKPICAVGHGVAALCcatne----------------drswVFDSYSLTGPSVCELvr----------aPGFarlp--l 167 human
YP_381151   124 asdKLIATICYAVGGLVwarnp---------------etgksIIDGKVICAHPRDWDftd--------plPYPldgaqsg 180 Synechococcus sp...
CAG08765    116 shqKPICAVGQGVSALCcvte-----------------gqkwIFSSYSLTGPSVFELvrqpd----fadlPLVv------ 168 Tetraodon nigrov...
Feature 1                                                                           
1ONS_A      231 --GEELKKMGMNIINdd-----------iTGRVHK---------DRKLLTGDSPFAANALGKLAAQEM 276 Escherichia coli
AAD37457    187 --QDRLVALGADFQEge----------pwAPFVIT---------DRNLITGQNPASSVPLAAELLNRL 233 Streptomyces griseus subsp. ...
AAF10068    210 --ADALTQGGAQVENge----------amKPNVVR---------DRELLTGQNPFSDEPFVAQLLEML 256 Deinococcus radiodurans R1
NP_436327   231 --QTALEAAGGDFVSnea---------pwASNVVT---------DRELITGQNPASAPAVATELLKRL 278 Sinorhizobium meliloti 1021
EAL18422    202 --ESTLREAGAEMVEglg---------ekIGRITV---------DRELISGGNPLAANALAEQVLKMV 249 Cryptococcus neoformans var....
EAL94924    185 --EDVLKEKGAIVENga----------awGSNVVR---------DRNLITGQNPQSSEDVAKEVIKAL 231 Arthrobacter sp. FB24
ZP_00686125 232 --QTALQRAGGIYSSnev---------pfTAHMVT---------DRELITGQNPASALDVAGQMLKRL 279 Burkholderia ambifaria AMMD
NP_872418   168 vvEDFVKDSGACFSAse----------pdAVHVVL---------DRHLVTGQNASSTVPAVQNLLFLC 216 human
YP_381151   181 naGTDLVTPGFNYPVqtivelavgekgrvLSNPYVlrscpcthyDWPFVTALSVESSTPFGYMIDNAL 248 Synechococcus sp. CC9605
CAG08765    169 --EDFVKDNGGSYTAsk----------edAVHVVI---------DRHLITGQNTQSTLLAVNNLNLLC 215 Tetraodon nigroviridis

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