1G2I,1G2I,1OI4


Conserved Protein Domain Family
GATase1_PfpI_like

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cd03134: GATase1_PfpI_like 
Click on image for an interactive view with Cn3D
A type 1 glutamine amidotransferase (GATase1)-like domain found in PfpI from Pyrococcus furiosus
A type 1 glutamine amidotransferase (GATase1)-like domain found in PfpI from Pyrococcus furiosus. This group includes proteins similar to PfpI from P. furiosus. and PH1704 from Pyrococcus horikoshii. These enzymes are ATP-independent intracellular proteases and may hydrolyze small peptides to provide a nutritional source. Only Cys of the catalytic triad typical of GATase1 domains is conserved in this group. This Cys residue is found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow. For PH1704, it is believed that this Cys together with a different His in one monomer and Glu (from an adjacent monomer) forms a different catalytic triad from the typical GATase1domain. PfpI is homooligomeric. Protease activity is only found for oligomeric forms of PH1704.
Statistics
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PSSM-Id: 153228
View PSSM: cd03134
Aligned: 48 rows
Threshold Bit Score: 188.522
Threshold Setting Gi: 76791108
Created: 6-Oct-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
conserved cysproposed
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:conserved cys residue [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1G2I_A        2 KVLFLT-ANEFEDVELIYPYHRLKEEGHEVYIASfe-rgTITGKhgy----svkVDLTFDKVnpeeFDALVLPGGr-aPE 74  Pyrococcus horik...
1OI4_B       25 KIAVLI-TDEFEDSEFTSPADEFRKAGHEVITIEkqagkTVKGKkgea---svtIDKSIDEVtpaeFDALLLPGGh-sPD 99  Escherichia coli...
NP_638111     9 TVAVLA-TSGFEQSELQEPKRLLESWGATVEVIApgddaQIRGWnhtdwgdsvpVDTPLAQAkpdrYDALVLPGGvinPD 87  Xanthomonas camp...
NP_390580     4 KIAVLV-TDQFEDIEYTSPVKAYEEAGYSVVAIDleagkEVTGKhge----kvkIDKAISDVdasdFDALLIPGGf-sPD 77  Bacillus subtili...
ZP_00377345   3 RVLIIA-TDGFEQSELMKPKKRLEEAGIDTTVASle-egEITGWkdknwgdsvkVDLTVEEVsaddYGALLLPGGqinPD 80  Erythrobacter li...
ZP_00472779  26 RVAILA-THGFEESELSAPRAALRSQGVEVHVVSpd-gkGIRAWaetdwgdtyeADKALSDVdstdYHALVLPGGlfnPD 103 Chromohalobacter...
ZP_00538923   4 KVAVVL-ADHFEDVEFTGPVDALKEAGHEVTVIGtekgaELVGKqeea---kvtVDVTIDETsssdFDALLIPGGf-sPD 78  Exiguobacterium ...
ZP_00555366   8 KILILS-THGFEQSELEVPLEQLRAAGATVHVASpe-sgEIKGWdggdwgasvpVDLPLSDVnaydYDALVLPGGqinPD 85  Jannaschia sp. CCS1
YP_289239    10 DVAVLIaPEGAEQVELTVPWDAIRDAGGRPRLIStt-ggRVQAFdhldradtftVDTTVHQVtahdFDALLLPGGvanPD 88  Thermobifida fus...
YP_301715     4 KVAIIL-ADEFEDIELTSPKEALENAGFETEVIGdtekhELVGKhge----kvtVDVSIADAkpenYDALLIPGGf-sPD 77  Staphylococcus s...
Feature 1                                  #                                                    
1G2I_A       75 RVRLNeka--vsIARKXFSEGKPVASICHGPQILISAGVLRGRKGTSYpgIKDDXINAGVeWVDAEVVVDg----nWVSS 148 Pyrococcus horik...
1OI4_B      100 YLRGDnrf--vtFTRDFVNSGKPVFAICHGPQLLISADVIRGRKLTAVkpIIIDVKNAGAeFYDQEVVVDkd---qLVTS 174 Escherichia coli...
NP_638111    88 NLRTNaqa--idFIRSVAASGKPVAAICHGPWLLVESGLVRDRKVTSWpsVKTDLSNAGGrWEDAEVVVDg----qLITS 161 Xanthomonas camp...
NP_390580    78 LLRADdrp--geFAKAFVENKKPVFAICHGPQVLIDTDLLKGKDITGYrsIRKDLINAGAnYKDAEVVVSh----nIVTS 151 Bacillus subtili...
ZP_00377345  81 ALRMNdtv--igLIREFNEANKPIAAICHAPWLLAEANIIRDRTVTGWpsIRTDLSNAGAnVIDSEAAVDg----nLITA 154 Erythrobacter li...
ZP_00472779 104 ELRLNdqa--ldFVRGFFEAGKPVAAICHAPWILINAGVVEGRRMTSVasVAEDLKNAGAeWVDEKVVVDn----gLVTS 177 Chromohalobacter...
ZP_00538923  79 LLREDdrf--vtFTEEFDTSKKPIFSICHGPQLMINAKIVKGRKMTGYksIRIDLENAGVsYEDKEVVVDd----nFVSS 152 Exiguobacterium ...
ZP_00555366  86 ILRTHega--vdIIRTFVDSGRIVAAICHAPWLLIEAGVVEGREMTSYpsIKTDMINAGAkWEDSEVAISn----gIITS 159 Jannaschia sp. CCS1
YP_289239    89 YLRTHera--vaFVREFFDTGRPVAAICHALWILIEAGVVRGRTLTSYpsLRTDLVNAGAtWVDREVVVStegpstLITS 166 Thermobifida fus...
YP_301715    78 HLRGDeegrygtFTKYFTQNDVPTFAICHGPLLLVDTDDLKGRTITGVinVRKDLSNAGAhVVDESVVIDn----nIVTS 153 Staphylococcus s...
Feature 1                       
1G2I_A      149 RVPADLYAWXREFVKL 164 Pyrococcus horikoshii
1OI4_B      175 RTPDDLPAFNREALRL 190 Escherichia coli K12
NP_638111   162 RKPDDIPAFTDAVAKA 177 Xanthomonas campestris pv. campestris str. ATCC 33913
NP_390580   152 RTPDDLEAFNRESLNL 167 Bacillus subtilis subsp. subtilis str. 168
ZP_00377345 155 RNPDDIPAFSNALIEQ 170 Erythrobacter litoralis HTCC2594
ZP_00472779 178 RTPKDLDAFNDKLIEE 193 Chromohalobacter salexigens DSM 3043
ZP_00538923 153 RQPDDIPAFNREMLAK 168 Exiguobacterium sp. 255-15
ZP_00555366 160 RNPDDLDAFVAKIIEE 175 Jannaschia sp. CCS1
YP_289239   167 RNPKDLPAFTKAIVEE 182 Thermobifida fusca YX
YP_301715   154 RVPDDLDDFNREIIKK 169 Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305

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