Conserved Protein Domain Family
GATase1_HTS

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cd03131: GATase1_HTS 
Type 1 glutamine amidotransferase (GATase1)-like domain found in homoserine trans-succinylase (HTS)
Type 1 glutamine amidotransferase (GATase1)-like domain found in homoserine trans-succinylase (HTS). HTS, the first enzyme in methionine biosynthesis in Escherichia coli, transfers a succinyl group from succinyl-CoA to homoserine forming succinyl homoserine. It has been suggested that the succinyl group of succinyl-CoA is initially transferred to an enzyme nucleophile before subsequent transfer to homoserine. The catalytic triad typical of GATase1 domains is not conserved in this GATase1-like domain. However, in common with GATase1 domains a reactive cys residue is found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow. It has been proposed that this cys is in the active site of the molecule. However, as succinyl has been found bound to a conserved lysine residue, this conserved cys may play a role in dimer formation. HTS activity is tightly regulated by several mechanisms including feedback inhibition and proteolysis. It represents a critical control point for cell growth and viability.
Statistics
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PSSM-Id: 153225
View PSSM: cd03131
Aligned: 22 rows
Threshold Bit Score: 204.032
Threshold Setting Gi: 48864758
Created: 7-Oct-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
conserved cysproposed active
Feature 1:conserved cys residue [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
ZP_00619967  38 IGLLNLMPKKi--qTENQFARLIGATPLQIEFSLIRmtehqtkn--taaahmeEFYrpFQEVrdekFDGLIITGAPIehl 113 Silicibacter sp....
ZP_00064065  29 ILLVNLMPNRl--qTEKQFTRLLSHLPINVRVTFAVpsehkir---hdtdaimTNYvtLNDIwhkkFDGMIVTGAPVdrm 103 Leuconostoc mese...
NP_766884    34 IGLINNMPDPalkaTERQFMKLLQAAAGPRRIRFHCfslpsvkrspeakwhveSEYsdLADLkrqrFDGLIVTGAEPvap 113 Bradyrhizobium j...
NP_772920    38 IGLINNMSDAalmsTERQLFDLLAAAAGRLCVRLHFytmeatprsewgrdyvrRYYrgIDDLlnrrLDGVIVTGAEPraa 117 Bradyrhizobium j...
NP_785970    30 ILILNLMPTKv--tTERQFLKRFAAGNTDVAVTFMYpashhfks--lpqavvaAHYvtLADIqdqyFDGLIVTGAPVetl 105 Lactobacillus pl...
ZP_00318634  32 ILILNLMPDRr--hVEKNFFDLFNSTKRKLNLTFIVpathkik---hdslavrQVYktFADIkdnyYDALLVTGAPLefi 106 Oenococcus oeni ...
YP_114313    38 IGLLNMMPDAaleaTERQFFRLVGGANPIVQFHMHPftieglprgdqaaehiaRYYesFDRIreegLDGLIVSGANVtqp 117 Methylococcus ca...
ZP_00384801   8 IGILNVMHDKa--dTKTRLQHVLTHTAIPVDLHFYYpmthyagr--tvpeavsSILdpLDIHevatMDGFIITGSPIetl 83  Lactobacillus ca...
ZP_00387696   5 IGILNLMHDKi--dTQKRFSHVIKEASPETEITYFYpqshyln----rprpqnWPAepLNLKtvakLDGFIVTGAPIell 78  Lactobacillus de...
YP_291279    38 IGILNIMPLGk--qYEFNLLHPLGLSPLQIEPIWIRlkshsyrt-wdlehlknLYVhwEEAMspipLDGLIITGAPVehl 114 Prochlorococcus ...
Feature 1                                   #                                                   
ZP_00619967 114 pFEDVTYWQELCEVFEWTQTNVhSTFGVCWGGMAMINYFHGVRKHMLDHKAFGCFRHQNlqp--tsPYLRGFSDDFVIPv 191 Silicibacter sp....
ZP_00064065 104 kFEQIDYWDEFRHLLEWRKTHVtESLFACWAAYGAGYAERNFPVKALSEKISGVFQASQif---krHSLLKDLENISMPq 180 Leuconostoc mese...
NP_766884   114 eLDQEPYWRDLTDLVDWARTNTrSTIWSCLAAHAAVLHLDGIARRRLPAKCHGIFDCEAvt---gdPLTHALPAPLKVSh 190 Bradyrhizobium j...
NP_772920   118 sLTDEPYWTTFAEIADWARENTvSSVFSCLAVHGAVLHLDGVTRHKLPTKCFGVFAQTKtr---hhPLMQDVPRTFRIPh 194 Bradyrhizobium j...
NP_785970   106 pFEAVDYWNELLTIIDWSRQHVsQTLFECWAAQAGLYAQFGIAKRAVAHKIFGIYSATStdvksplISGLNAGGLLKMPq 185 Lactobacillus pl...
ZP_00318634 107 dFSQIDYFPEFLEILKWRKKHVaFSTFECWSAMALSRIEYHLDYSLERKKVSGIYETPV-------YSLLKGTKTIQVPq 179 Oenococcus oeni ...
YP_114313   118 hLQQEAFWQPLTEVFDWARSNVtSILCSCLATHALFQYSYGVERTHLGFKRWGVYSHRVvep--lhPLVADINTRFDVPh 195 Methylococcus ca...
ZP_00384801  84 eFDQVHYIAEVRTLLKTLSQHVpNQLYLCWGGMVALNYFFGISKLILPHKLFGVYPQTIle---phPLLKGLKNDFKSPh 160 Lactobacillus ca...
ZP_00387696  79 pFSEVDYWEELTKLFDLLKAKNiPQLYVCWGAMAALYYFYGIGKHPMPEKIFGIYPQEIla---paPLLSGLITGFLAPh 155 Lactobacillus de...
YP_291279   115 pFEEVHYWKELVNLIEEAKLKCaSTLGLCWAGFAMAYMAGVEKENFNKKLFGVYPMRSLvp---shSLMGTQDDEFFCPq 191 Prochlorococcus ...
Feature 1                                
ZP_00619967 192 SRWTEMSqseid---aapgLTTLLG 213 Silicibacter sp. TM1040
ZP_00064065 181 SRYFTVPnfgv-----arrLKVAGD 200 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
NP_766884   191 SRLNEIVesdl----eqagYQVLTR 211 Bradyrhizobium japonicum USDA 110
NP_772920   195 ARWNEVQeedlsd-cgysvLTWSAE 218 Bradyrhizobium japonicum USDA 110
NP_785970   186 SRHTALVmper----lpagLQVVAD 206 Lactobacillus plantarum WCFS1
ZP_00318634 180 SRYFKINgla------kswQVITND 198 Oenococcus oeni PSU-1
YP_114313   196 SRYNEIFredm----eaagLRVLVE 216 Methylococcus capsulatus str. Bath
ZP_00384801 161 ARYAEMDvrgih---adprLTINAT 182 Lactobacillus casei ATCC 334
ZP_00387696 156 ARYAEMNrseie---qekaLTINAA 177 Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365
YP_291279   192 SRHAGLPdiemekaeqkgkLRLLAH 216 Prochlorococcus marinus str. NATL2A

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