1OX4,1FYE,1T0B,1SOA,1G2I,1GPM,1I7Q,1JDB,1L9X,1O1Y,1ONS,1R9G,1S1M,1T3T,1VHQ,1GGE,1KWK,1QVW,1G2I,1GPM,1I1Q,1K9V,1KA9,1KEE,1OI4,1PDW,1PV2,1Q7R,1QDL,1SY7,1U9C,1VCO,1WL8,2A9V,1J42,1PS4,1PE0,1VCN


Conserved Protein Domain Family
GAT_1

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cd03128: GAT_1 
Click on image for an interactive view with Cn3D
Type 1 glutamine amidotransferase (GATase1)-like domain
Type 1 glutamine amidotransferase (GATase1)-like domain. This group contains proteins similar to Class I glutamine amidotransferases, the intracellular PH1704 from Pyrococcus horikoshii, the C-terminal of the large catalase: Escherichia coli HP-II, Sinorhizobium meliloti Rm1021 ThuA, the A4 beta-galactosidase middle domain and peptidase E. The majority of proteins in this group have a reactive Cys found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow. For Class I glutamine amidotransferases proteins which transfer ammonia from the amide side chain of glutamine to an acceptor substrate, this Cys forms a Cys-His-Glu catalytic triad in the active site. Glutamine amidotransferases activity can be found in a range of biosynthetic enzymes included in this cd: glutamine amidotransferase, formylglycinamide ribonucleotide, GMP synthetase, anthranilate synthase component II, glutamine-dependent carbamoyl phosphate synthase (CPSase), cytidine triphosphate synthetase, gamma-glutamyl hydrolase, imidazole glycerol phosphate synthase and, cobyric acid synthase. For Pyrococcus horikoshii PH1704, the Cys of the nucleophile elbow together with a different His and, a Glu from an adjacent monomer form a catalytic triad different from the typical GATase1 triad. Peptidase E is believed to be a serine peptidase having a Ser-His-Glu catalytic triad which differs from the Cys-His-Glu catalytic triad of typical GATase1 domains, by having a Ser in place of the reactive Cys at the nucleophile elbow. The E. coli HP-II C-terminal domain, S. meliloti Rm1021 ThuA and the A4 beta-galactosidase middle domain lack the catalytic triad typical GATaseI domains. GATase1-like domains can occur either as single polypeptides, as in Class I glutamine amidotransferases, or as domains in a much larger multifunctional synthase protein, such as CPSase. Peptidase E has a circular permutation in the common core of a typical GTAse1 domain.
Statistics
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PSSM-Id: 153222
View PSSM: cd03128
Aligned: 1728 rows
Threshold Bit Score: 29.4762
Threshold Setting Gi: 68189474
Created: 26-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
conserved cys
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:conserved cys residue [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1OX4_A        7 VHVIDvesgnl----qsltnAIEHLg---------YEVQLVKspkdf---------------------nisgtSRLILPG 52  baker's yeast
AAL93975      4 IAIFLfegaelf-eiasftdVFGWNnvvglkefrdIKVETISykesikctwggelra-ekiitednvenfyeyDALVIPG 81  Fusobacterium nu...
NP_852993    11 IAAIVatgvddl-eliiptdLWRRAr---------FIVDYISiekknsvtlaqgtsi--kcgltidksnlmqyNAIYLPG 78  Mycoplasma galli...
NP_757456    11 IAIMCsiesndm-divipydIWTRAg---------IIVETVSnekkksiflqsgtki--tcseeiekvnldkfNALYIPG 78  Mycoplasma penet...
ZP_00868328   3 VLIAIppekfrdeellipvkKFQEAgvt-----yeLASTHVGvvmgvlgkkakvnr----tfedvllsgvddyYALMIVG 73  Methanospirillum...
ZP_00867355   3 ILIAVpptqyhdkelaqvmaVFDQNk---------IPYEFVSrekgqakgtfggrvyvplsfeeviltredefDALAILG 73  Methanospirillum...
NP_247962    34 VLMVIapkdfrdeelfepmaVFESNg---------LKVDVVSttkgecvgmlgnki---tvektiydvnpddyVAIVIVG 101 Methanocaldococc...
ZP_00563544   7 ILMVIaqenfrdeeflkpkkVFEDSg---------AKVTVASnttkkakgvlgkkv---spdisisdvniddyDAISITG 74  Methanococcoides...
AAM05062     10 ILLVVaqeqfrdeecfvpkqLFEAAg---------ANVTVAAefretakgalggti---kpdirisdariedyDAIVISG 77  Methanosarcina a...
ZP_00868007   7 VLIVIapvkfrdeelsepikYLEKAg---------IGYDIIStqtglaigmlggkvliektvrdvseagitpyAGILIVG 77  Methanospirillum...
Feature 1                                            #      
1OX4_A       53 VgnyghfvdnlfnrgfekpIREYIes----gkPIMGICVGLQAL 92  baker's yeast
AAL93975     82 GfgkanffkdndneifkklIKYFSen----nkVIVAICSAVINL 121 Fusobacterium nucleatum subsp. nucleatum ATCC 25586
NP_852993    79 Glgskrfgd---dqklthyLSKFNekdqttprYILAINESPIIL 119 Mycoplasma gallisepticum R
NP_757456    79 Gkgcqrlltd-rnervinkIVKFAgde--gkkWLFTAGAASTVL 119 Mycoplasma penetrans HF-2
ZP_00868328  74 Gpgtqvhlw--nnrhlielVTIFNtr----qkVVAAIDNAPIVV 111 Methanospirillum hungatei JF-1
ZP_00867355  74 Ghgaqahfw--nnpdllelVKIFRih----rkVIGAISTAPVVM 111 Methanospirillum hungatei JF-1
NP_247962   102 Gigskeylw--nntklielVKEFYnk----nkVVSAICLSPVVL 139 Methanocaldococcus jannaschii DSM 2661
ZP_00563544  75 Gggakqylw--dnkelqeiVRKANeq----gkIIGAICIAPVVL 112 Methanococcoides burtonii DSM 6242
AAM05062     78 Gpgsrkylw--dnqylsglVKEADsl----kkVVSAICISPVIL 115 Methanosarcina acetivorans C2A
ZP_00868007  78 Gggspdhlw--nnkplqtlVQEFDaa----gkILSAICLSTVVL 115 Methanospirillum hungatei JF-1

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