1FOH,1PN0


Conserved Protein Domain Family
PHOX_C
?
cd02979: PHOX_C 
Click on image for an interactive view with Cn3D
FAD-dependent Phenol hydoxylase (PHOX) family, C-terminal TRX-fold domain; composed of proteins similar to PHOX from the aerobic topsoil yeast Trichosporon cutaneum. PHOX is a flavoprotein monooxygenase that catalyzes the hydroxylation of phenol and simple phenol derivatives in the ortho position with the consumption of NADPH and oxygen. This is the first step in the biodegradation and detoxification of phenolic compounds. PHOX contains three domains. The substrate and FAD/NAD(P) binding sites are contained in the first two domains, which adopt a complicated folding pattern. The third or C-terminal domain contains a TRX fold and is involved in dimerization. The functional unit of PHOX is a dimer, although active tetramers of the recombinant enzyme can be isolated when overproduced in bacteria.
Links
?
Statistics
?
PSSM-Id: 239277
Aligned: 34 rows
Threshold Bit Score: 93.8918
Created: 16-Mar-2005
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
dimer interface
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:
  • Structure:1PN0; Trichosporon cutaneum Phenol Hydroxylase dimer; contacts at 3.5A between monomers
  • Structure:1FOH; Trichosporon cutaneum Phenol Hydroxylase dimer; contacts at 3.5A between monomers
  • Comment:The functional unit of phenol hydroxylase (PHOX) is a homodimer.
  • Comment:The C-terminal TRX-fold domain of PHOX is involved in dimerization. Residues from the two other domains also contribute to the dimer interface.
  • Citation:PMID 9634698
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1                    # #                                                              
1FOH_A    481 GTRFKSQPVVRHs--EGLWMHFGDRLVTDG-RFRIIVFAGKAtd----------aTQMSRIKKFSAYLDSensvislyt- 546 Trichosporon cutaneum
1PN0_D    482 GTRFKSQPVVRHs--EGLWMHFGDRLVTDG-RFRIIVFAGKAtd----------aTQMSRIKKFAAYLDSensvisryt- 547 Trichosporon cutaneum
CAB91725  460 GRLFINSTVTRVv--DANVVHLEQEIPLNG-SFRLFIFAGSLts----------pAQKSALESLAAGLQKpdsffsrfqr 526 Neurospora crassa
NP_792453 362 GAGIPRFRRGDKerqLLLNYRGRSLSLNSTdNVDGKVQAGDRapda------tcsQQRCGSTQLFDVFRGghft------ 429 Pseudomonas syring...
CAE76270  454 GCLLPPAKVTRYl--DSNPVDVQLDIPMLG-QFRIYLLMWDVh------------QSRIFLDGFCAALNSpdslmnqlsa 518 Neurospora crassa
EAK85170  511 GARLLPATVTRWk-dCNPISVLESLAFADA-RFTVFACTGLLptseilgflkhvaRPGGLHQQLRSKHPAsevqpdrshl 588 Ustilago maydis 521
EAK95154  510 GRRLNSSNIICQa--NGQPVHIADRLASDG-RFRVLIFPGDVvk---------ypENLKTLHKFQDCLDSkhffakkft- 576 Candida albicans S...
CAG86406  532 GRRFDTTLVVTHs--DARPIQIVDRMLSDG-RWRILNFSGDVkk----------dSQMDVLNRISDYIYAkdsfrnkyt- 597 Debaryomyces hanse...
YP_235454 376 GTGLPTFRRGDKerqLLLNYRASSLSVNSAdNVDGKVQAGDRapda------tcsQQHGGRTRLFDVFRGghft------ 443 Pseudomonas syring...
EAL93849  447 GRAFTPTTVTRLa--DANFVHLEQEIPANG-AFRIFIFAGKQa------------NTKKAITDFGANLEKersflssyrr 511 Aspergillus fumiga...

Feature 1                                              #            #  ##                  #  
1FOH_A    547 ----------------------pkvsdrnsrIDVITIHSCHR------------DDIEMHDFPAPal-----hpKWQYDF 587 Trichosporon cutaneum
1PN0_D    548 ----------------------pkgadrnsrIDVITIHSCHR------------DDIEMHDFPAPal-----hpKWQYDF 588 Trichosporon cutaneum
CAB91725  527 pdid-------------tvshherhnphskfFTLATVFAAKRa-----------EVEISRDLPALl--------ARYRDN 574 Neurospora crassa
NP_792453 430 ----------------------------llaFGAHAIAATQAf-----------DSHDKHCLRTFavr---pkhVGDDRD 467 Pseudomonas syring...
CAE76270  519 aanisyakqprlpapediylrperytavshlFTFGLITTMPK------------SEIEITDLPALf--------QDSRWT 578 Neurospora crassa
EAK85170  589 l--------------------pglldphrpvLQLVGITMHSHvsmqlaasqtdlCCYSTRAIDANvvqfnpdwwMCDDRA 648 Ustilago maydis 521
EAK95154  577 ----------------------pvnafansvIDVITVHGSSR------------HEVEIFDFPDFthpl-dfkrRCDYWR 621 Candida albicans S...
CAG86406  598 ----------------------pinahedsvFDVLTIYSSKR------------SSLELFDFPRIcvpq-dhkkRRNYWK 642 Debaryomyces hanse...
YP_235454 444 ----------------------------llaFGTQAIAAMNTfg---------sRDERFLQAVAVrp-----ahLSNDRY 481 Pseudomonas syring...
EAL93849  512 ide---------------isfferhlphsklFSICLIYAAQK------------NEVDVEAIPQIl--------RDYHHH 556 Aspergillus fumiga...

Feature 1                                                                           
1FOH_A    588 IYADcdsw----hhphPKSYQAWGVDe-TKGAVVVVRPDGYTSLVTDl---------eGTAEIDRYFSGI 643 Trichosporon cutaneum
1PN0_D    589 IYADcdsw----hhphPKSYQAWGVDe-TKGAVVVVRPDGYTSLVTDl---------eGTAEIDRYFSGI 644 Trichosporon cutaneum
CAB91725  575 VYADdrwdrr-vpdakAAAHAKMGFDe-DKGGVVVVRPDGYVGFVCRlee-----ggkTVEALEKYFGAF 637 Neurospora crassa
NP_792453 468 GLVDe----------aGQACEAYGVSa-EENIIFLVRPDGYVGLVAVg---------dFVAAIKRYLARV 517 Pseudomonas syringae pv. tom...
CAE76270  579 FYLDdipel--dtrgqLCTNKWLGSLgpGEVAIVNVRPDGYVGSIGRwdssvddageeAAKWLDTYYDRF 646 Neurospora crassa
EAK85170  649 SLSPypdqmgkpsmlvHPAHEKWRVDp-NRGALVVVRPDSHVALVTSsi--------yEWKAVEAFFASL 709 Ustilago maydis 521
EAK95154  622 LYSGvgdty---hegsVDIYDFYGISk-SEGVILVIRPDSHVAQVSQys-------idGLKEVDNYFNNF 680 Candida albicans SC5314
CAG86406  643 LHSGiettl---hegaCDAYKKYGIDt-EKGCVVVVRPDGYVSLVTDfs-------ldGYKKISNFFDEF 701 Debaryomyces hansenii CBS767
YP_235454 482 GLVDe----------tGQARDAYGVSl-QENIIFLIRPDGYVGLTATd---------dFVPAVQRYLATL 531 Pseudomonas syringae pv. syr...
EAL93849  557 IYADdipdvr-vplakFAAHEKLGFDp-EKGGVVVTRPDSHVACTVQlve-----gsgTVDALNAYFNSF 619 Aspergillus fumigatus Af293

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap