3CZ8


Conserved Protein Domain Family
GH18_CFLE_spore_hydrolase

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cd02874: GH18_CFLE_spore_hydrolase 
Click on image for an interactive view with Cn3D
Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
Statistics
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PSSM-Id: 119353
View PSSM: cd02874
Aligned: 31 rows
Threshold Bit Score: 314.203
Threshold Setting Gi: 24214908
Created: 21-Jul-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             #                             ##                                           
3CZ8_A         7 IAGTLSFYVLRnpdl---drelINDYAPYSSSISIFEYHIApngdi--------anqlNDAAAIETTWqr-------rVT 68  Bacillus subtil...
YP_075619    132 GPVVVAYYAEDwegdv-ralnaLRRAGARVDRVVNFALELHadgs----------vttRSYPALAAAAae------lgVP 194 Symbiobacterium...
ZP_01171329  258 LTWEAVYSENPdh--------aALPSMPGVNVVSPTWFELAgedgs--------vknlGSGDYVKWAHgr-------gYQ 314 Bacillus sp. NR...
YP_001039286 256 LAWDMIYTRREdh--------sSLGEMKGLDVISPTWFQVKnakge--------linrAYSKYVDWAHsr-------gYQ 312 Clostridium the...
CAE09818      23 GLERLVYMVDRsds-----lslLQERIDSIEVVAPQVFSFTlegt---------vygeVDPRIIALAKer-------gKK 81  Wolinella succi...
YP_001106110  59 VVASLPFWNLGngt------atVVQNRTAVNEVSPWMYGLGddgr---------ltiqYSPAQATEVAghlqrlreaqIP 123 Saccharopolyspo...
YP_074307     73 GFWIAGYYVDYdsds----lesVRLNAHHLDQVIVFGYGFDaegna-------vgkdqTLIRGVTSHQk--------rVL 133 Symbiobacterium...
YP_822092     17 QPKALFYLNDSpas-----irsFQANADKIDVIVPTVYSVDaagl---------vwgkPDPRVLETSRkf-------rVA 75  Solibacter usit...
NP_712389    117 ADENTSFRASTwfsd----yeaMKKTVHLYNEIHPFIYGMKgretnngdlysswgssqKHERISELRKln------pnIK 186 Leptospira inte...
CAL82759     169 VVAVDGFYTLEegetlpssyesFVTNINLISEICLFMFRIDpsdataie-kfgdftdeYVKELVNIAHrn-------nVR 240 Clostridium bot...
Feature 1                        #                                  # # #                        
3CZ8_A        69 PLATITNLts----ggFSTEIVHQVLNNPTARTNLVNNIYDLVSTRGYGGVTIDFEQVSAADRDLFTGFLRQLRDRLq-a 143 Bacillus subtil...
YP_075619    195 VYGLVHNMqn----gqFSAEIARAVLSDPARRSRAVADMLAIAREHGLAGIDIDIENVPGDLRAGYTALAAEAAAVLk-p 269 Symbiobacterium...
ZP_01171329  315 VWGLFSNA--------FDAELTHQTFKDFETRQKIIRSLLHYSQIYKLDGINLDIENVNQEDGPLVTQFVREATPYFhqa 386 Bacillus sp. NR...
YP_001039286 313 VWALLSNDf-------TDSEMTSKFLNNTDARDNLIREILAYAALYNLDGINIDFENMYISDRDVFTQFVREIAPLLreq 385 Clostridium the...
CAE09818      82 LMPLVMNEn-------FEKEKIKAFLANPKAQERLIGSLIKIALKEGFGGWQLDFEHLGITSKEAYTQLVRQLYARLkeh 154 Wolinella succi...
YP_001106110 124 IVPSLANIte----gnWSYEPVARMLHDPELMRRHVFEIVELVKRENYAGIDIDYENLRAGDREQFTRFVTDLGRALhae 199 Saccharopolyspo...
YP_074307    134 LFGNFSDAg-------FDPDLAHRILTDPVVQERAMSSMLDEAGRLGVSGIQIDFENIPPEDREAYTQFLRRLKERLepl 206 Symbiobacterium...
YP_822092     76 LMPIIVNPg-------FKQDMIHALMANAEARRRMNATLLAECRKYGYYGIQFDFENINYLDRDLLASMVKETSAALgrg 148 Solibacter usit...
NP_712389    187 IIPTIFRWen----kyEKISENIGMNGRNDIRDKHLSNILYELDTYGYDGIDIDYEGMSCEKKEKFEEFIVILSKEIhkr 262 Leptospira inte...
CAL82759     241 ALAVVHNLlyrpggttKAKDLVKSLVSTRENRQIFINNLISLIEKYNFDGVNIDIEDVYIEDKDNLSSLYLEMGREFr-r 319 Clostridium bot...
Feature 1                                                                 # ##                   
3CZ8_A       144 GGYVLTIAVPaktsdn--------------------ipwlrGYDYGGIGAVVNYMFIMAYDWHHa---gSEPGPVAPITE 200 Bacillus subtil...
YP_075619    270 GGIRLTLSVPaktwddt------------------tsnwggAFDYAALGEIADEIAIMAYDEHTw---gLPHGPVASLPW 328 Symbiobacterium...
ZP_01171329  387 GLTVSMDITFisssg----------------------nwsaFYERDKLAGIVDYLAVMAYDEHWat--sPVAGSVASLPW 442 Bacillus sp. NR...
YP_001039286 386 GLVVSVDVNDi-----------------------------qCYDKKALSEAVDYIMYMSYDQHWst--sPVAGSVAQVSW 434 Clostridium the...
CAE09818     155 HLSLSIAVVPprgeepknay-----------gakvfedwvsPYDLKALAEHSDFLTLMAYDQHTg---gSTPGPIASRAW 220 Wolinella succi...
YP_001106110 200 GKTLSVAVFAktsdag-------------------ydernvAQDYAAIGQVADQVRLMGYDFHWg---tSRPGPVAPVGW 257 Saccharopolyspo...
YP_074307    207 GLTLSVAAAAktsdtt--------------------sgwggATDYAAIGKIVDHFYIMAYDQHWl---gGEPGPVASLDW 263 Symbiobacterium...
YP_822092    149 GFKLSIATVPnegdepgksdy----------trwvfqnwrgAFDLRQLAPYVDFISLMTYDQHTr---nTPPGPVAGMTW 215 Solibacter usit...
NP_712389    263 GKILSVAVHPktpakkesmkackglkdkikmdyaenwrgpmTHDYAFLAKYADRIKIMAYELHPrkyrnPGPGPQAPNTW 342 Leptospira inte...
CAL82759     320 KGYFLSASIPsrvsdep------------------fnpfsdPFDYRIIGSAVDEFIVMLYNEHGwp--gSGPGPVVSIGW 379 Clostridium bot...
Feature 1                                 #                                                      
3CZ8_A       201 IRRTIEFti--aqVPSRKIIIGVPLYGYDWIipy----qpgTVASAIsnqNAIERAMRy-------------------qA 255 Bacillus subtil...
YP_075619    329 VERVVAYav--tqIPPEKILLGIPAYGYDWIag------trQVVGGLstgGAYSLAARh-------------------gA 381 Symbiobacterium...
ZP_01171329  443 VESNLQAll--evVPKEKLILGVPLYARLWEekd----eggISSEALsmeQVKEWLTEt-------------------gA 497 Bacillus sp. NR...
YP_001039286 435 QEKIVKRvleqegVPREKLLLGIPFYTRLWKetvdesgkkkLTSSALtmkQAKNLIIEn-------------------nA 495 Clostridium the...
CAE09818     221 VEGIIQEal--iwVPKEKLSLGIPLYSRLWHagike-ekmrGVAKSLtfaGAMDWLERq-------------------gA 278 Wolinella succi...
YP_001106110 258 IRDVLSYat--svVPRERIVLGVPLYGYDWVdg-------qGTSVTWl--QAFKLATEh-------------------kV 307 Saccharopolyspo...
YP_074307    264 TERVIRYai--gvMPSQKILLGVPLYGYEWAlep----elaDTNAAYgpgRMQRRAAQf-------------------gA 318 Symbiobacterium...
YP_822092    216 VEKLAAYae--sqMPKAKISLGVALYGRRWSagmrd-kdpaVAASTIyaaDAVDLAKAi-------------------dA 273 Solibacter usit...
NP_712389    343 IKSIIEYak--arVPAKQLYMAIPTYGYDWAlnc----nskIKSVYY---QDAVRKKEfgihrqptnidqilantknssS 413 Leptospira inte...
CAL82759     380 MNRVLNYti--trVPRNKVVAAVSVFGFDFNlt-------tGRNTYVtyaGAIEIAKRy-------------------gK 431 Clostridium bot...
Feature 1                                                                 #             
3CZ8_A       256 PIQYSae-yqSPFFRYSDq------qGRTHEVWFEgVRSMSRKMQIVREYrLQAIGAWQLTLAEGHHHHHH 319 Bacillus subtilis subsp....
YP_075619    382 EVRWDde-aqVPWFRYVAd-------GAEHIVYFEnSRSAAAKLRLVGRYgLGGIAIWRLGLNEDGLWEVV 444 Symbiobacterium thermoph...
ZP_01171329  498 VPVYDeasgqNYAEYYSVk------dKATYKIWLEdEMSLRKRAEIADRHgLAGVGSWSRYFADQSAWLAL 562 Bacillus sp. NRRL B-14911
YP_001039286 496 KVEWDee--sGQFYAEYTk------dNTNYRLWLEdANSINLRTSLVHKYrLAGTCAWSIYFVSEDIWDVL 558 Clostridium thermocellum...
CAE09818     279 ETLWLer--dGVKWAMGEr------gGVKEYLFMEeARSLALKVELAKRYgLRGISLWRLGQEDPRIWDEL 341 Wolinella succinogenes
YP_001106110 308 PTFYDar-tqSPWFAYTDa------qGRRHEVWFEnTASSKAKFEAARGSgIRGVYLWMYGYEDTGTWPEL 371 Saccharopolyspora erythr...
YP_074307    319 EVVWDpvhaeNRAVFLTD--------EGQRVAWFPdERSLEAKLRLAYQYnLKGIAVWRVGLEPDDWWHRM 381 Symbiobacterium thermoph...
YP_822092    274 TVQWDpl--eHAPWFYFYr------dGIREYVFYNdARSFEDRYAMARERgLHGISAWILGSEDPGLWRSL 336 Solibacter usitatus Elli...
NP_712389    414 WINLSkfswvHTGKTYEDpsiwykseGCDRVAFYMnRKAFEDKMNLLRQYdLGGFSFWQLISDNDPGINDY 484 Leptospira interrogans s...
CAL82759     432 DIIFDee-tkTPMFSYVDe------nGNNHEVWFEnAESIYAKAELAFNKgIKGIALWRLGMEDNKIWDSM 495 Clostridium botulinum A ...

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