1HKK,1WAW,1E9L,1HJV,2PI6


Conserved Protein Domain Family
GH18_chitolectin_chitotriosidase

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cd02872: GH18_chitolectin_chitotriosidase 
Click on image for an interactive view with Cn3D
This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.
Statistics
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PSSM-Id: 119351
View PSSM: cd02872
Aligned: 63 rows
Threshold Bit Score: 486.681
Threshold Setting Gi: 13537190
Created: 6-Jul-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:the chitolectins bind chitin but lack the catalytic residues and do not have chitolytic activity, in contrast to the chitotriosidases which are also included in this alignment.
  • Structure:1HKK_A; Human macrophage chitotriosidase binds allosamidin
    View structure with Cn3D
  • Comment:Allosamidin is thought to resemble the structure of a reaction intermediate that is unique among the glycoside hydrolases.
  • Structure:1WAW_A; Human macrophage chitotriosidase binds argadin, a chitinase inhibitor
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            #   #                                                                        
1HKK_A          3 LVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTn--hQLSTTEWn-------DETLYQEFNGLKkmnpkLKTLL 73   human
XP_001641806   23 RVCYFTNWAQYRPDPVKFLPKDIDPLLCTHIVYAFAKIDpatnKIGTYEWn-------DDRLYKEINDLKlknpsLKTLL 95   starlet sea a...
AAK93412      125 IVCYYTNWSQYRVKIGKFVPEDIPADLCTHIIFAFGWLKk--nKLSSYESndet-kdnVPGLYERMMTLKkanpkLKILL 201  fruit fly
AAK93412      560 VFCYLTSWSAKRPGAGKFQPENIDPKLCTHIVYAFATLQ----DYKLTEAtd-----dDPENYESVIALRdnnpdLQILL 630  fruit fly
BAC06447      473 VVCYFTNWSAKRKGKGHYEPEDIDATLCTHVIYAFANIKe--fKIVPTEPvdegdgsaKKGFWERIVALKtknpqLKVML 550  Haemaphysalis...
BAC06447       46 IVCYFTNWAQYRQGDGKFLPEDIDPTLCTHIIYAFGWMKk--hKLSSFDAaddt-kngKKGLYERVIDLKkknpsLKVLL 122  Haemaphysalis...
CAI96026       24 RVCYYTNWAQYRNGIAKFEPSHIDPSLCTHIIYAFGKLEd--nGITNFEWn-------DETRYVEVNAFKrnntgLKTLL 94   Pacific oyster
XP_001632508   23 RGCYYTNWSQYRPKGGTFWPEDIDPFLCTHILFSFAKVNqtthKLDIYEEn-------DHELYQRINALKkinpkLKTQI 95   starlet sea a...
XP_001641690   21 RVCYHTNWSQYRQGRAKFWPEDIPADLCTHLMYSFAKINqk-nELAMYEWn-------DDKLYPRFNALKqknpeLKTLL 92   starlet sea a...
NP_001108099   41 RVCYYTNWSQYRPGNGKYIPDDVDPFLCTHIVYSFVEIGsn-nQLKLREWn-------GDQLIDSLLTKKqtnpdLLITA 112  Ciona intesti...
Feature 1             ##                                        # ##                              
1HKK_A         74 AIGGWNFGtq---kFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPgsqg-spavDKERFTTLVQDLANAFQQea 149  human
XP_001641806   96 AVGGWNHEsgpvspFSQMVASKSNRTTFISSLLQLSDKYDFDGFDLDWEYPasrgnsppqDKQHFTILCEEMLDAFKRka 175  starlet sea a...
AAK93412      202 ALGGWSFGtq---kFKDMSSTRYTRQTFVYSAIPFLRKRGFDGLDMDWEYPkg-----sdDKKNFVLLLKELREAFEAea 273  fruit fly
AAK93412      631 AIGGWAFGst---pFKELTSNVFRMNQFVYEAIDFLRDYKFNGLDVDWEYPrg-----aeDRVAYVSLLKELRVAFEGea 702  fruit fly
BAC06447      551 AVGGWMLGsa---pFREVTENSYRQSLFVFNAIDFLREKGFDGLDVDWEFPrg-----aeDKKKLAGLIKELRVAFDGeg 622  Haemaphysalis...
BAC06447      123 AVGGWSFGtq---rFKEMASNSYNRRLFIFSALNFLRRRKFDGLDLDWEFPrg-----neDKKNFVELVRELREAFEAea 194  Haemaphysalis...
CAI96026       95 AMGGWTAGsk---pYSDMAASPENRRTFINASISWLRKYDFDGLDMDWEYPanrg-gvpeDFNNFPILLKEILEAFTEea 170  Pacific oyster
XP_001632508   96 AVGGWTHEekn-spFSKMVATKEKRAIFIKSAIETLRTHGFDGLDLDWEYPgmrggspksDKGRFTLLCRELREAFEAea 174  starlet sea a...
XP_001641690   93 AVGGWNHEnan-spFSRMVKTAATRKVFIDSVIRILRTWGFDGLDLDWEYPatrggsppeDKQRFTVLCQELMNTFIAeg 171  starlet sea a...
NP_001108099  113 AVGGWNFGte---rFTAVCENEANMRMFAKTSVDFLRQFKFDGLDMDWEYPgsrg-sppeDKQRFTRLLEILMEEYEAea 188  Ciona intesti...
Feature 1                        ##                      # ##    #                                
1HKK_A        150 qtsgkerLLLSAAVPAGqtyVDAGYEVDKIAQNLDFVNLMAYDFHGSWe-kVTGHNSPLYkrqeqsg-----aaaslnvD 223  human
XP_001641806  176 adtdkprMLLTAAVSAGhgtVDAAYEVHKLAGILDWINLMTYDLHGPWe-pYTGHHTALVgppgd----------kltvS 244  starlet sea a...
AAK93412      274 qelkkprLLLSAAVPVGpdnIRGGYDVPAIASYLDFINLMAYDFHGKWe-rETGHNAPLYapstdse-----wrkqlsvD 347  fruit fly
AAK93412      703 kssglprLLLTAAVPASfeaIAAGYDVPEISKYLDFINVMTYDFHGQWe-rTVGHNSPLFalesatg-----yqkkltvD 776  fruit fly
BAC06447      623 laskkprLILSMAAPASfeaISAGYDVEELNKHVDMINMMTYDFHGDWe-rQVGHHSPLFplytass-----fqkkltvD 696  Haemaphysalis...
BAC06447      195 kekklprLLLTAAVSAGaetIRGGYDVPAVAAYVDFLNVMSYDFHGKWe-sMTGHNSPLYaqanett-----wrkqlcmD 268  Haemaphysalis...
CAI96026      171 ktsgksrLLLTAAVGVGksvADTAYNIPEMSKYLDFISLMAYDLRGGWe-kTTGFNAALYrssads-------sdeynvA 242  Pacific oyster
XP_001632508  175 kasgkerMLLTAAVAAGlwtIKGAYDIADIAEPLDWINLMTYDLHGTWe-yKTGHHTAMGpdgdkltlpfaiwywmnnrD 253  starlet sea a...
XP_001641690  172 kqtgkprLLLTAAVSAGkntIDKAYEVEKIGKILDLINLMAYDLRGKWe-pHTGHHTSLEgppge----------iltvK 240  starlet sea a...
NP_001108099  189 lstgnprLLLTAAVPAGpstIEAGFEINAVCSILDLVHIMTYDFHGGSwekNTGHNAALYpsppgti------qndfniE 262  Ciona intesti...
Feature 1                               # #                            #                          
1HKK_A        224 AAVQQWLQKGTPASKLILGMPTYGRSFTLasss-dtrvGAPATGSGTPGPFTKEGGMLAYYEVCsw---kgATKQRIQDq 299  human
XP_001641806  245 YAVKYWMEKGMPCGKIALGMANYGHAFEL---------SDPTKTALGAPANVNKGRSYPYYELCkl----pLTKVTDNPa 311  starlet sea a...
AAK93412      348 NAASLWVKMGAPKEKLVIGMPTYGRSFTLanpd-khgpNAPASGGGREGVYTKEGGFLAYYEICeml-lngAVYVWDDEm 425  fruit fly
AAK93412      777 YSAREWVKQGAPKEKLLIGMPTYGRSFELvndt-qfdiGSPSSGGGKAGKFTNEAGFLSYYEVCsflaadnTTLVWDSEq 855  fruit fly
BAC06447      697 YSAGEWVRKGASKEKLLVGIPTYGRTFTLgdnn-ltdvGAPATAGGRPGNYTGETGFLSFFEICdll-rsgATLVWDNEq 774  Haemaphysalis...
BAC06447      269 FGVKTWERLGAPKEKIVVGTGTYGRTFTLanpn-nngmNAPSSGGGDAGQFTKEAGFLAYYEICdml-kkgADYVWDEEq 346  Haemaphysalis...
CAI96026      243 FAVDYWLRKGTPKEKLILGLATYGRSFKLqden-nfgvGAPATGAGPQGKYVAEDGFLPYYDICarqvqrvGETYRDEKa 321  Pacific oyster
XP_001632508  254 TWEKPGIRKGMPANKIVLGLATYGRAFGLpspatngldSARDYQYTPKGKYTDAEGFLAYYEICkr----gLTVVHKNKa 329  starlet sea a...
XP_001641690  241 FATQYWIDMGAPPSKIALGLGTYGRSFKLtfke-kngiGAPANGNPPRGPYTRESGFLSYYEICdmmpkmkTMKTDQSEv 319  starlet sea a...
NP_001108099  263 ESVNIWLTNGCDPKKLVLGVPTYGRTYTLldpl-kfgfGANATGPGDAGTYTREAGFLAYYEICpymndpaANFIWSDYa 341  Ciona intesti...
Feature 1                                                   #                            
1HKK_A        300 KVPYIFr----dNQWVGFDDVESFKTKVSYLKQ-KGLGGAMVWALDLDDFAGfsCNq-gRYPLIQTLRQEL 364  human
XP_001641806  312 KAPYGYh----gSQWIAYDDVTSLGRKVELIKK-ENLLGAMFWAIDLDDFGNv-CGq-gAHPLMGAVRYML 375  starlet sea anemone
AAK93412      426 KVPYLVd----gDQWVGFDDERAIRNKMHWIKS-NGFGGAMVWTIDMDDFKGevCGgnvKYPLIGAMREEL 491  fruit fly
AAK93412      856 QVPFAYr----gNQWVGFDDERSLKTKTEWLKE-QGFGGIMVWSIDMDDFSGr-CGs-gKYPLLTALNDEL 919  fruit fly
BAC06447      775 MVPYAYk----dDQWVGFDDQRSLKLKVQWLKQ-AGYGGVMVWSVDLDDFKGt-CTg-hSYPLLTAIKEEL 838  Haemaphysalis longicornis
BAC06447      347 LVPYAYl----gNQWVGFDDERSIRAKMQWIKM-NGYAGAMVWTVDMDDFRGr-CTt-kTWPLIGAMAEEL 410  Haemaphysalis longicornis
CAI96026      322 QTPFFVq----eNIWVGFDDQLSIYTKVNDLVIsKQLGGAMIWALDFDDFNNi-CGy-gKYPISRVMTDTL 386  Pacific oyster
XP_001632508  330 NAPYGYk----gKDWIGFDDPNSLVYKIDNVVKkNQLRGVMFWAIDLDDFSGehCGq-gKYPLMSAVKNYL 395  starlet sea anemone
XP_001641690  320 RAPYGYvkqdwaDVWVGYDDERSLQLKVEEVIKaKGLAGAMFWALDLDDFDGssCGk-gNYPLMNAVKKYL 389  starlet sea anemone
NP_001108099  342 MAPAVAv----gDQWISFDDVNSIEYKINYMKS-LGLGGTMVWAVDNDDFQAt-CGy--KNHLLRQMYFLL 404  Ciona intestinalis

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