2YY6,3D6J


Conserved Protein Domain Family
HAD_PPase

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cd02616: HAD_PPase 
Click on image for an interactive view with Cn3D
pyrophosphatase similar to Bacillus subtilis PpaX
This family includes Bacillus subtilis PpaX which hydrolyzes pyrophosphate formed during serine-46-phosphorylated HPr (P-Ser-HPr) dephosphorylation by the bifunctional enzyme HPr kinase/phosphorylase. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319797
Aligned: 14 rows
Threshold Bit Score: 249.889
Created: 19-Aug-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on a putative haloacid dehalogenase-like hydrolase from Bacteroides fragilis and on other haloacid dehalogenase superfamily members with structure
  • Structure:3D6J: Bacteroides fragilis putative haloacid dehalogenase-like hydrolase binds a phosphate ion in the active site, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              #####                                 #                                   
2YY6_A         1 XRVILFDLDGTLIDSaKDIALALEKTLkelgleeyypdNVTKYIGgGVRALLEKVlkdkf----reeyVEVFRKHYlenp 76  Aquifex aeolicus
3D6J_A         6 YTVYLFDFDYTLADSsRGIVTCFRSVLerhgytgitddXIKRTIGkTLEESFSILtgitda-dqlesfRQEYSKEAdiyx 84  Bacteroides fra...
ACA59067       3 VRAVLFDLDGTLIDTiPLIRWTFERVFadfgl-pwengEVLHTVGlPLREIAARYmp---------drADEFMERYaafq 72  Candidatus Desu...
Q9K6Y7         3 INTVLFDLDGTLINTnELIISSFLHTFetyypgkygrkDAIECIGpPLTDSFKRLdper-----veemVATYRKHNhahh 77  Bacillus halodu...
Q8XIY6         2 IKAVLFDLDGTLINTnDLILKSFKHTFktmldlepseeEITMNYGrPLQEIFKSYde---------nrIEEMINCYrkin 72  Clostridium per...
Q8R821         3 ITAVLFDLDGTIIDTnQLIIKSFVYTVekhlgykigaeEVIPYFGePLPLTLQRFsk---------dkWEIMLKTYrdyn 73  Caldanaerobacte...
A9VQ75         3 INTVLFDLDGTLINTnELIISSFLHTLnhyysnqykreDVLPFIGpSLHDTFSKIda---------skVEEMITCYrqfn 73  Bacillus weihen...
YP_430846      2 FAGVLFDFDGTLVDTsELVIKSFQHTLapylgrvvapkEVYPYFGvTLKEGLAAFmpdh-----ldemLHEYRRYSaahf 76  Moorella thermo...
Q8ENK3         3 IRTILFDLDGTLIDTnTLIKASFEHTFkeynl-nfsneEILKFNGpPLVDTFNKIdetk-----adrmITTFREHNireh 76  Oceanobacillus ...
YP_002249875   3 IELIIFDLDGTLVDScKDITQALNYCFkkrgiegfsqeEVKKMVGeGVNRLIEKAlqlrklslpvqdlVECFINYYkkhi 82  Thermodesulfovi...
Feature 1                                       ###                              #               
2YY6_A        77 ----vvyTKPYPEIPYTLEALKSkgfKLAVVSNKleELSKKILDIlnlSGYFDLIVGGDTFGEKKPSPTPVLKTLEILGE 152 Aquifex aeolicus
3D6J_A        85 ----nanTILFPDTLPTLTHLKKqgiRIGIISTKyrFRILSFLRNhxpDDWFDIIIGGEDVTHHKPDPEGLLLAIDRLKA 160 Bacteroides fra...
ACA59067      73 ktrfrelTRAYPGAVETLATIKSagyRTGVVTSKrrEPALASLALtglDQHIEAVVTADDVTKPKPDPEPVFKALELLHT 152 Candidatus Desu...
Q9K6Y7        78 ----dklVEPYEGVYETVKTLHEqgfKLAIVTTKirETAMKGLKLfglDEFFDVIVALDDVENVKPNPEPLEKAMNALGA 153 Bacillus halodu...
Q8XIY6        73 lelhddeCKEFADVDLMLKTLKSkgiKIGVVTSKksDMAERGAKLmgiFKYFDTFITPEITTKHKPDGEPVLKACENLGV 152 Clostridium per...
Q8R821        74 ekyhdryTKIREDVKEVLARLKEegiKTAVVTSKrrELAKRGLKLfelDKYFDVLVGLEDTEKHKPEPDPVLKALELLKS 153 Caldanaerobacte...
A9VQ75        74 hehhdelVEEYETVYETVQELKKqgyKIGIVTTKarQTVEMGLKLsklDQFFDVVVTIDDVEHVKPHPEPLQKALELLDA 153 Bacillus weihen...
YP_430846     77 ----ddlVRPCPGVREGLQRLQQagiKLGVVTSRlrDTTLYGLELcglTSFFPVIVAAEDVTSHKPGPEPVRYGLELLGV 152 Moorella thermo...
Q8ENK3        77 ----dnfVTAFPHVYDTLEELQNrniSLGIVSTKmrHTVHMGLELtgiSKFFSTIITYDDVTHAKPHPEPVQMAMQKLGA 152 Oceanobacillus ...
YP_002249875  83 ----adfSIVYPNVRETLEKLQGi--KKAVISNKltELSIKTLETlglLEYFDFIAGSDLFAERKPSPVPIIETIKRFNT 156 Thermodesulfovi...
Feature 1                ##  ##                                          
2YY6_A       153 EPEKALIVGDTDADIEAGKRAGtKTALALWGYvkl---nsqIPDFTLSRPSDLVKL 205 Aquifex aeolicus
3D6J_A       161 CPEEVLYIGDSTVDAGTAAAAGvSFTGVTSGXttaqefqayPYDRIISTLGQLISV 216 Bacteroides fragilis NCTC 9343
ACA59067     153 RPQNAAYIGDSWYDVVAGKQAGvTTVGATWGIasreqlaehAPDIIVDSWDEFLAN 208 Candidatus Desulforudis audaxviator MP104C
Q9K6Y7       154 KKEETIMVGDNSHDILGGKNAGvKTAVVGYAIrgedyvrqfDPDYVLRSMPDLLDI 209 Bacillus halodurans C-125
Q8XIY6       153 SPSEALMVGDSPYDILAGKNAGaKTCGVKYTAlpleklgesNPDFYVDKPLEILDL 208 Clostridium perfringens str. 13
Q8R821       154 PREEALMVGDSPYDILSARSAGvRSVAVKWSVlpfellkkeKPDYFIEDMWQLLKI 209 Caldanaerobacter subterraneus subsp. te...
A9VQ75       154 KPEETLMVGDNHHDIVGGQNAGtKTVAVSWTLkgrayleayKPDYVLDKMSDLLPI 209 Bacillus weihenstephanensis KBAB4
YP_430846    153 EAGAAAMIGDSPHDIQAARAAGvTSVAAGWSRvprdqilaaGPEVLVASMTEFVDF 208 Moorella thermoacetica ATCC 39073
Q8ENK3       153 HPEHTLMVGDNHHDIVSGQRANvQTAAVAWSLkdtnylksfHPDYIIEDIKDIITI 208 Oceanobacillus iheyensis HTE831
YP_002249875 157 SPDKTIIVGDSELDIKAGTLAGvKTVAVTYGYreka--llkNADFIIDKFSDLITI 210 Thermodesulfovibrio yellowstonii DSM 11347

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