1SB7,1Z2Z,1SB7,1SI7,1SZW


Conserved Protein Domain Family
PseudoU_synth_TruD_like
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cd02552: PseudoU_synth_TruD_like 
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Pseudouridine synthase, TruD family.
This group consists of eukaryotic, bacterial and archeal pseudouridine synthases similar to Escherichia coli TruD and Saccharomyces cerevisiae Pus7. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). E. coli TruD and S. cerevisiae Pus7 make psi13 in cytoplasmic tRNAs. In addition S. cerevisiae Pus7 makes psi35 in U2 small nuclear RNA (U2 snRNA) and psi35 in pre-tRNATyr. Psi35 in U2 snRNA and psi13 in tRNAs are highly phylogenetically conserved. Psi34 is the mammalian U2 snRNA counterpart of yeast U2 snRNA psi35.
Links
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Statistics
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PSSM-Id: 211326
Aligned: 78 rows
Threshold Bit Score: 131.556
Created: 14-Mar-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitePermutation of
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:1SZW_B, Escherichia coli TruD, PDB D80 is key conserved residue
  • Comment:The E. coli TruD residue F131 is conserved in the TruD family, while it is a conserved tyrosine in the other pseudouridine synthase families
  • Structure:1sb7_a, active site of Pseudouridine Synthase
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
1SB7_A     37 GLLKANpEDFVVVEDLgfepd----------------------------------------------------------- 57  Escherichia coli
NP_187133  59 GILKQRyADFIVNEVDmdgnvvhltsldlppemavkeedkakscdnvpqnytsavesfksissvtdaerleilinqvtsg 138 thale cress
O74343     67 GIIKARfTDFAVFEVDtdgnivhltdmeahdpilskatgdketedakdssnqdisndqkapsfkeqepatlpilpndlqs 146 fission yeast
EAA66013   65 GILKKRyTDFLVNEILpsgkvlhltnttapntndeatpvqadkkpaedkpkepetpaeklpapvefqlaeedealldtlf 144 Aspergillus nidula...
XP_326283  35 GDIRKRyTDFLVYEIRkdgstvhlreyeeqqpsvpkpapakptpetvapstepeieikpvsdedrkklvdligesatekl 114 Neurospora crassa
AAW45215  105 GVIKQRfTDFLVNEIGldgkvlhlkditkpqepgsnekdkeaatvteeeiangqtksttevasqseepevavdlpenlrf 184 Cryptococcus neofo...
XP_760173  62 GIIKQRfTDFMVNEIDllgnvcrltsldppagkllaqsrplaassdqlqaaaetaekeqaqtqldesawpvvaeeeeklk 141 Ustilago maydis 521
EAA77527   34 GEMRTRfSDFQVNEISedgsvlhlqqigiageetpatetkkeangesgvketkheepaqdaekqpsnetaaetkpaaqpe 113 Gibberella zeae PH-1
NP_916327  65 GALKQRySDFVVHEVAldgslvrltsfdlpdgecvdakeggmdeaqalesfrl--------------------------- 117 Japanese rice
BAD82076   65 GALKQRySDFVVHEVAldgslvrltsfdlpdgecvdakeggmdeaqalesfrllcgdadcealrgflervseggdsdvsp 144 Japanese rice

Feature 1                                                                                     
1SB7_A        --------------------------------------------------------------------------------     Escherichia coli
NP_187133 139 tdgevlpvvldps------------------------------------------------------------------- 151 thale cress
O74343    147 iipkgignefiqslh----------------------------------------------------------------- 161 fission yeast
EAA66013  145 gtqntkkivalhkkala--------------------------------------------------------------- 161 Aspergillus nidula...
XP_326283 115 iklneavqakqtisage--------------------------------------------------------------- 131 Neurospora crassa
AAW45215  185 aahpqwststtlklrphfsdetiislhqlveqgkdappkgdsgwggrkkdtesaseenaereeekkeegeeaafndsask 264 Cryptococcus neofo...
XP_760173 142 effsvesieslkemwakgkqgtnmatganatv------------------------------------------------ 173 Ustilago maydis 521
EAA77527  114 qvnevsaedaailegltdqrfare-------------------------------------------------------- 137 Gibberella zeae PH-1
NP_916327     --------------------------------------------------------------------------------     Japanese rice
BAD82076  145 iilsadadkthrs------------------------------------------------------------------- 157 Japanese rice

Feature 1                                                                                     
1SB7_A        --------------------------------------------------------------------------------     Escherichia coli
NP_187133 152 -------------------------------------sdkskrtaihnfvkenfkflvtdtvdgpdstskcirvrvnsga 194 thale cress
O74343    162 -----------------------------------klsvgeitdpislilpentppmdkgqrtilhqfirnnfsglesst 206 fission yeast
EAA66013  162 --------------------------------npktkpsdlgrlntvvvndrdqrikmhqairrifnsqiesstdsegmm 209 Aspergillus nidula...
XP_326283 132 ---------------------------------rtvvfdpitdraqraslhqeirrifdnrietlanntgvitatpskws 178 Neurospora crassa
AAW45215  265 grgqgrdrgrgrgkergrgrggrggrgggrnadsgswwltyederepviskeeraaahkilrevfpgmfesstrevkgee 344 Cryptococcus neofo...
XP_760173 174 ------------------vsaasivtkpledkvqrtqvhklirelfhgklatdscqvrprgaraveedrvaqanaasnst 235 Ustilago maydis 521
EAA77527  138 -------------------------lievykagsgpdndkrktvtseamddrakrgqvhqeirrifksrvdtntgddgai 192 Gibberella zeae PH-1
NP_916327 118 -----------------------------------------------------------------------------lcg 120 Japanese rice
BAD82076  158 ------------------------------------evhdffkrnfealltdtvehsdgiqrcirvrlkpgrrerrdvdg 201 Japanese rice

Feature 1                                                                                     
1SB7_A     58 -----------------------------gegeHILVRILKNGCNTrfvaDALAKflk---------------------- 86  Escherichia coli
NP_187133 195 nnnrgnrskkrkergdkpfdsrgsehwpehvgkFLRFHLYKENKDTqealGLIGKmlg---------------------- 252 thale cress
O74343    207 kgngtftvskttrknqprsrrdprlswkalggeYCHFHLYKENRDSmdclGKIARllk---------------------- 264 fission yeast
EAA66013  210 visvaanrnkknpqgggggrerprvnwdelggqYLHFTIYKENKDTmeviSFIARqlk---------------------- 267 Aspergillus nidula...
XP_326283 179 knnrvgghgprannsnnsrsrdqsksftqlggeYLHFTMYKENKDTmdavNTIARllk---------------------- 236 Neurospora crassa
AAW45215  345 gqrlvikwssgagqssfhgkgarrapdaprlpaYIHFTLHKSNRETmdalSHLQRlln---------------------- 402 Cryptococcus neofo...
XP_760173 236 isaastliavrwanrsdrrsgdglseeaassppYIHFLLHKTNRDHqeamGMLAEslrlggggagrgrgrgrggfagrgg 315 Ustilago maydis 521
EAA77527  193 iatlipprkanskkrgrgggrgggardekpageYLHFTLFKENRDTmdavNQISRflk---------------------- 250 Gibberella zeae PH-1
NP_916327 121 dadcealrgflervseggdsdvspiilsadadkTHRSEVHDFFKRNfealLTDTVehsdgi------------------q 182 Japanese rice
BAD82076  202 rgrnrkgtgssgwrddkpfdsrgsiiwpdhlgkFIRFHLYKENKDTqealGKIGKmlg---------------------- 259 Japanese rice

Feature 1                ####                                                                 
1SB7_A     87 --ihAREVSFAGQKDKHAVTEQWLCarvpgkexp-----------------------------dlsafqlegCQVLEYAR 135 Escherichia coli
NP_187133 253 --vqPKSFGFSGTKDKRSVSTQRVTvfkqqaskl-----------------------------aalnkrlfgIKVGDFCN 301 thale cress
O74343    265 --vpTRTLSIAGTKDRRGVTCQRVAihhvrasrlaql------------------------nsgslknstygFLLGNYSY 318 fission yeast
EAA66013  268 --mnPKSFQFAGTKDRRGVTVQRACayrlqadrl-----------------------------aklnrtlrnAVVGDFEY 316 Aspergillus nidula...
XP_326283 237 --vkATNFGFAGTKDRRAGTVQRISvhrqrasnl-----------------------------iwlntrlpnIKVGDFSH 285 Neurospora crassa
AAW45215  403 --vqAKDLTVCGTKDKRAVTVQRVClkrsgrnlqtvwralngv-----------kqgwrteqqaveergdrgVRVGDFEY 469 Cryptococcus neofo...
XP_760173 316 grppTKDLAVAGTKDKRAVTVQRVSlrrnrktledvyrlvnginadvsaskvkgkgkgrtvldavtsradrgIRIGHLEY 395 Ustilago maydis 521
EAA77527  251 --vkPQVIGYAGTKDRRASTVQRCSvrymrprnl-----------------------------agingriwgVSTGDYEY 299 Gibberella zeae PH-1
NP_916327 183 rcirPRSFGFAGTKDKRAVTTQQVTvfkvqasrl-----------------------------valnskligIKVGDFSY 233 Japanese rice
BAD82076  260 --lqPRSFGFAGTKDKRAVTTQQVTvfkvqasr-------------------------------lvalnsklIGIKVGDF 306 Japanese rice

Feature 1                                                                                     
1SB7_A    136 HKRKLRlGALKGNAFTLVLRevsnrddve----------------qrlidicvkGVPNYFGAQRFGiggsnlqgaqrwa- 198 Escherichia coli
NP_187133 302 VKEGLLlGQLMGNRFTITLRgvvadseeti--------------kqsaeslgkdGFINYFGLQRFGsgsvpthhvgaall 367 thale cress
O74343    319 KNSNLRlGDLKGNEFHIVVRnvitpkekvv---------------ealnslkehGFINYFGLQRFGtssvgthtigvrll 383 fission yeast
EAA66013  317 QPHGLElGDLYGNEFVVTLRecevpginiqdpasava-ktkelvntslknlyqrGYFNYYGLQRFGsfatrtdtvgvkil 395 Aspergillus nidula...
XP_326283 286 SKEPLQlGQHGGNEFVITLKnckplggagcsvdqrvr-klreavefglaylqrhGYINYYGLQRFGtyaigthllgmkvl 364 Neurospora crassa
AAW45215  470 SDKYLElGMLKGNRFLITLRnveaesveei--------------drtmesvrdlGFINFYGMQRFGtssmpthvtglfil 535 Cryptococcus neofo...
XP_760173 396 AHEPLKlGQLKGNEFTITLRnvrpssssasvdkaf-----lasvrrsmdvirdrGFINYFGMQRFGtgaipthhigilvl 470 Ustilago maydis 521
EAA77527  300 KDKPIYlGQLLGNEFVIAIKscqmvgensdesiaeqveklkknvdsslshmnehGWINYFGHQRFGthevgthqigqlil 379 Gibberella zeae PH-1
NP_916327 234 VKEGLAlGQLMGNRFTITLRsvvtesedvi--------------kaaldglitnGFINYYGLQLQRlkkypgnylqalma 299 Japanese rice
BAD82076  307 RSVVTEsEDVIKAALDGLITngfinyygl-----------------qlqrlkkyPGNYLQALMAIPrtlrlmyvhsyqsy 369 Japanese rice

Feature 1                                                                                     
1SB7_A        --------------------------------------------------------------------------------     Escherichia coli
NP_187133 368 kgewkdavdmildpretdillhdlffltlrfflqrhvvn--------------------dareyyketgdidgtlrqlpr 427 thale cress
O74343    384 qsdwkgavdlilsprpehtgsvkeaidlwhst----------------------------------hdaeaslrilprrm 429 fission yeast
EAA66013  396 qddfkgacdaildysphilaaaqaelgqgegegatptnissed------------karalaihifrttdrvtdalekmpr 463 Aspergillus nidula...
XP_326283 365 kedyegvlddilhvedhflheafdleeqepahtndfhnsrdd-------------ynrakaitiwkttrnaekalailpk 431 Neurospora crassa
AAW45215  536 rsnwsaaldsllflregehpdcvaarlawled----------------------------------gdyvkalekmprra 581 Cryptococcus neofo...
XP_760173 471 rndfktaidllfeprppsspdaddgdsldllkakql--------------------------ykegeldkaysvipkncv 524 Ustilago maydis 521
EAA77527  380 gdkyeeavksllhyddeiaqkaeageipeepskrdeylrnq---------------acmlfltnkdvrraidimprrfsa 444 Gibberella zeae PH-1
NP_916327 300 iprtlrlmyvhsyqsylwnhaasmrvqkygisrvv---------------------------egdlvykkeapfeqgalk 352 Japanese rice
BAD82076  370 lwnhaasmrvqkygisrvvegdlvykkeapfeqgalkatseddgqtmssemnaccetlpeemiqsvkiadsedlskslyt 449 Japanese rice

Feature 1                                                                                     
1SB7_A    199 -------------------qtntpvrdrnkrSFWLSAARSALFNQIVAERLkkad------------------------- 234 Escherichia coli
NP_187133 428 ylvaerailqclkkcpgnylqalkgiprtlrMMYVHSYQSYLWNNAASLRVknyg------------------------- 482 thale cress
O74343    430 iaessiletwsrsgnqtdylgafqriprhlrSIYPHAYQSYVWNRVASWRIknlg------------------------- 484 fission yeast
EAA66013  464 kfsaesniirhlgrskndylgalqtiprnlrLMYVHAYQSLVWNLAVGERWrlyg------------------------- 518 Aspergillus nidula...
XP_326283 432 rfsseaaiirhlgknskdfmgallsitrgmrMMYIHAYQSYVWNHMASKRWamyg------------------------- 486 Neurospora crassa
AAW45215  582 vaersiwefwkrggrmedkvgalgviprnlrTMYVHAYQSYVWNLIVSERIkmsa------------------------- 636 Cryptococcus neofo...
XP_760173 525 aekhildkmrssrwnkgdwmgafgniprtlrLMYVHAYQSYVWNRLVSERVakfg------------------------- 579 Ustilago maydis 521
EAA77527  445 encifrhlnrqgaqsrrdfigslvhitrglrSMYLHAYQSYVWNHAVSRRWelhg------------------------- 499 Gibberella zeae PH-1
NP_916327 353 atseddgqtmssemnaccetlpeemiqsvkdGINLRESVHGVEDFSITRMKggyrrviqrpidfewdlitytdekiplve 432 Japanese rice
BAD82076  450 fedvvlplpgsetlfpgnevagiyheiamkdGINLRESVHGVEDFSITRMKggyrrviqrpidfe------wdlitytde 523 Japanese rice

Feature 1                                                                                     
1SB7_A    235 vnqvVDGDalqlagrgswfvatteelaelqrrvndkelxitaalpgsge------------------------------- 283 Escherichia coli
NP_187133 483 tsqvVLGDlvstkvdaakrmvdsltsehnesseealdcdqvddtavvdlpaersdlvkvvsiedleagt---------ys 553 thale cress
O74343    485 -drpVVGDlvystesnglsqkspivdpeapdlledlpvssklsarpieedevnnfsiydi-------------------- 543 fission yeast
EAA66013  519 -drvVEGDlvlihehrdkdgnssyttpapgagasgetttidadgeiiivpqehdsafavedtftraraltaaeansglys 597 Aspergillus nidula...
XP_326283 487 -ptvVEGDlvlvsndqspadadsednenfyaqaraltqediasgkytifdvvlptpg----------------------- 542 Neurospora crassa
AAW45215  637 -tepLVGDlvieksdddepvdpenvpahrkdrhgrtkswktsssptvrrltaeditnkthti------------------ 697 Cryptococcus neofo...
XP_760173 580 slqaVEGDlvfadkrdedgwqevggedeerpngtdgeaeddddlrngggkinplatwqrpikvlttcdvq-------sdq 652 Ustilago maydis 521
EAA77527  500 -envIPGDliiapaettplvsgqdqdgddiinpveddedtpvrarpltaeeaasgnytifdi------------------ 560 Gibberella zeae PH-1
NP_916327 433 tdldVLSKtkplevnellsdgissctshd--------------------------------------------------- 461 Japanese rice
BAD82076  524 kiplVETDldvlsktkplevnellsdgissct------------------------------------------------ 555 Japanese rice

Feature 1                                                                                     
1SB7_A    284 ------------------wgtqrealafeqaavaaetelqallvrekveaarraxllyPQQLSWNWwdd----------- 334 Escherichia coli
NP_187133 554 tsdivlplpgsrviypsndiaeiyhdlakkdgisltesihgvkefsitsmtggyrrvfQKPIDFEWelltytdsnkplae 633 thale cress
O74343    544 -------vlplpgrnviypknetfdiyksvmneasldplnmsrkdrelslpgdyrkllVRPENMEFnfikydnmeqqlil 616 fission yeast
EAA66013  598 ifdivlplpgfdvlyppnkmtdfykefmgssrgggldpfnmrrkwkdaslsgsyrkvlSRMGRDYSvdvvlysrdeeqfv 677 Aspergillus nidula...
XP_326283 543 ----------ydviyprneigkayvdfmkktenggldpydmrrrqkefslsgnyrhllGRFIGEPQyairvynddteqmy 612 Neurospora crassa
AAW45215  698 ----fdvvmplpgfdvdypggevgelydsilkadgldkdrmrreqreyslpgsyrlilLRPLSLTWthlqytdpdlplvq 773 Cryptococcus neofo...
XP_760173 653 wsiydvilplpgssidlpvgwmsdlytsiltndslthtqltcskfpdyqlngsyrpmlVKPRNLTYsitsytdpdiplth 732 Ustilago maydis 521
EAA77527  561 ----vlptpgydvvypendmgefykefmgrdengnldpykmrrmrrefslpgryrkimNRFLATPSaevraysddteqmh 636 Gibberella zeae PH-1
NP_916327 462 --------------------------------------sgleasldasesingaslvvAEAKSIGSsdml---------- 493 Japanese rice
BAD82076  556 -----------------------------------shdsgleasldasesingaslvvAEAKSIGSsdml---------- 590 Japanese rice

Feature 1                                                                                     
1SB7_A        --------------------------------------------------------------------------------     Escherichia coli
NP_187133 634 tdldripmnkpvekvgsteeiedes------------------------------------------------------- 658 thale cress
O74343    617 tdkd---------------------------------------------------------------------------- 620 fission yeast
EAA66013  678 rtdlenltl----------------------------------------------------------------------- 686 Aspergillus nidula...
XP_326283 613 ptdldfcnaanakkkaatakartsppllanwahftnntaqydqaitnerrrkasqspesgvvvaretwiqtgldgsdkrv 692 Neurospora crassa
AAW45215  774 sdede--------------------------------------------------------------------------- 778 Cryptococcus neofo...
XP_760173 733 tdeelcld------------------------------------------------------------------------ 740 Ustilago maydis 521
EAA77527  637 ptdldnikasksanrkrsrddaesdstakk-------------------------------------------------- 666 Gibberella zeae PH-1
NP_916327     --------------------------------------------------------------------------------     Japanese rice
BAD82076      --------------------------------------------------------------------------------     Japanese rice

Feature 1                                                                                     
1SB7_A        --------------------------------------------------------------------------------     Escherichia coli
NP_187133     --------------------------------------------------------------------------------     thale cress
O74343        --------------------------------------------------------------------------------     fission yeast
EAA66013      --------------------------------------------------------------------------------     Aspergillus nidula...
XP_326283 693 kiarhteeyetkvpvekinemvadnatngiisssatetptlakglsdlyyesaaqqtvvksedgttkqeqdvmlteesta 772 Neurospora crassa
AAW45215      --------------------------------------------------------------------------------     Cryptococcus neofo...
XP_760173     --------------------------------------------------------------------------------     Ustilago maydis 521
EAA77527      --------------------------------------------------------------------------------     Gibberella zeae PH-1
NP_916327     --------------------------------------------------------------------------------     Japanese rice
BAD82076      --------------------------------------------------------------------------------     Japanese rice

Feature 1                                                                                     
1SB7_A        --------------------------------------------------------------------------------     Escherichia coli
NP_187133 659 -------------------------------------------mksdtnphdsgetnlkdqtdsneaekdtgnpdseqtq 695 thale cress
O74343    621 ----------------------------------------------------------------rlenrsisvssevgkh 636 fission yeast
EAA66013  687 -----------------------------------------------------------ktrdggdvdlekkegksegdk 707 Aspergillus nidula...
XP_326283 773 skadtmevdaatheatpqsnqsaladtensmvisnsekdgttktkeaanlivpvlhspsenplasvdnsevirsspdask 852 Neurospora crassa
AAW45215  779 ----------------------------------------------------------------ilslnppaandpegkf 794 Cryptococcus neofo...
XP_760173 741 ------------------------------------------------------------aslsnsiqddqddnsssskf 760 Ustilago maydis 521
EAA77527  667 ---------------------------------------akvedsteiemtdasgqagegetgpsentseatpavqepsk 707 Gibberella zeae PH-1
NP_916327 494 ------------------------------------------------------------------------------ek 495 Japanese rice
BAD82076  591 ------------------------------------------------------------------------------ek 592 Japanese rice

Feature 1                            
1SB7_A    335 vtVEIRFWLPAGSFATSVVRELI 357 Escherichia coli
NP_187133 696 maLKMALTLPSSCYATMAIRELL 718 thale cress
O74343    637 taVTLKFVLPSSAYATMALREAL 659 fission yeast
EAA66013  708 laVVLKFQLGSSQYATMALRELM 730 Aspergillus nidulans FGSC A4
XP_326283 853 laVILKFQLRSSNYATVVLRELM 875 Neurospora crassa
AAW45215  795 raVKVDMQLGASTYATMVLREIT 817 Cryptococcus neoformans var. neoformans JEC21
XP_760173 761 taLTLRFQLPSSSYATMLMREAL 783 Ustilago maydis 521
EAA77527  708 iaVIVKFQLGRSAYATVALRELM 730 Gibberella zeae PH-1
NP_916327 496 laIKLAFTLPASSYATMAIRELM 518 Japanese rice
BAD82076  593 laIKLAFTLPASSYATMAIRELM 615 Japanese rice

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