3DO8


Conserved Protein Domain Family
PPAT_CoAS

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cd02164: PPAT_CoAS 
Click on image for an interactive view with Cn3D
phosphopantetheine adenylyltransferase domain of eukaryotic and archaeal bifunctional enzymes
The PPAT domain of the bifunctional enzyme with PPAT and DPCK functions. The final two steps of the CoA biosynthesis pathway are catalyzed by phosphopantetheine adenylyltransferase (PPAT) and dephospho-CoA (dPCoA) kinase (DPCK). The PPAT reaction involves the reversible adenylation of 4'-phosphopantetheine to form 3'-dPCoA and PPi, and DPCK catalyses phosphorylation of the 3'-hydroxy group of the ribose moiety of dPCoA. In eukaryotes the two enzymes are part of a large multienzyme complex . Studies in Corynebacterium ammoniagenes suggested that separate enzymes were present, and this was confirmed through identification of the bacterial PPAT/CoAD.
Statistics
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PSSM-Id: 173915
View PSSM: cd02164
Aligned: 18 rows
Threshold Bit Score: 164.375
Threshold Setting Gi: 16554495
Created: 7-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site(T/H)XGH motif
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on similarity to bacterial PPAT and homologous proteins.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              ##                           #                                             
3DO8_A          2 KVALGGTFEPLHEGHKKLIDVAIKLggrDITIGVTSDrmARARIrs--vlPFAIRAENVKRYVMRKYgf----ePEIVKI 75   Archaeoglobus...
NP_001004577  181 DVVVGGTFDRLHGAHKTLLNISCLManrRFIIGVCDQelLKNKVlkeliePYDQRVEKLHDFLNDVKps---lkYDIVPL 257  zebrafish
Q13057        193 RGAVGGTFDRLHNAHKVLLSVACILaqeQLVVGVADKdlLKSKLlpellqPYTERVEHLSEFLVDIKps---ltFDVIPL 269  human
NP_490766     101 KVVLGGTFDRLHNGHKVLLNKAAELasdVIVVGVTDKdmIIKKSlfemiePVEFRIKKVVDFVEDISgt---akCLAEPI 177  nematode
NP_647985     146 SVVLGGTFDRIHLGHKIFLTQAVLRtckRLVVGVTTSamTKGKTlpdlilPVEERIARLREFLVDIDdt---lqYEIVPI 222  fruit fly
AAH74248      179 HVVVGGTFDRLHNAHKILLSVCCLLadtRLLIGLAHKqlLDNKTlkeliePYEQRVEKLSQFLVDVKps---llYDIVPI 255  African clawe...
EAK86000      781 VVALGGTFDHLHVGHKILLTMASLIaeqKIIVGVTDDamLTKKSdaavlePIDERIAAVNAFIGLVRlpfteldQQVVKL 860  Ustilago mayd...
EAK90683      236 RTLFAGTFDRLHPGHKVNITVATWYakeLVIIGITDKlfNANKSdkdiiqDFSFRSANVHSFIFSLSpd---isVAILRI 312  Cryptosporidi...
AAQ65117       17 AVVLGGTFDRLHDGHRMFLKAAAELardRIVVGVCDGpmLTKKQfsdmiqPIEERMRNVETYVKSIKpe---lvVQAEPI 93   thale cress
AAT92628      144 VTALGGTFDHIHDGHKILLSVSTFItsqRLICGITCDelLQNKKykeliePYDTRCRHVHQFIKLLKpd---lsVELVPL 220  baker's yeast
Feature 1                          #                                                            
3DO8_A         76 TNPYGKtld-vdfEYLVVSPETYEMALKINQKREELGKRKITIVKVdwmma-------------edgkPISSTRIKRG 139  Archaeoglobus f...
NP_001004577  258 SDPFGPsitepelQCIVVSEETRKGGEAVNKRRVQNGLAELVLYEIpllkdah--------radieeeKISSSSLRTR 327  zebrafish
Q13057        270 LDPYGPagsdpslEFLVVSEETYRGGMAINRFRLENDLEELALYQIqllkdlr--------hteneedKVSSSSFRQR 339  human
NP_490766     178 IDPFGPstrikdlEAIVVSRETIKGADAVNKKRNEQGMSQLDVIIVelvegsd---------eilketKISSSSRRRE 246  nematode
NP_647985     223 DDPFGPtqvdpdlDMIVVSAETLRGGQKVNEVRSAKQLRELEIFVIdivesnv--------hdgihetKVSSSNTRID 292  fruit fly
AAH74248      256 SDPYGPsitdpdlKCIVVSEETQKGGLSVNRKRQENELCELAIHEIklvkdar--------haeneeeKISSSSFRNR 325  African clawed ...
EAK86000      861 EDVAGPaateanlQALVVTDETLSGAEFIDGKRKENGLASVENWVIgvvgsqgqtel-kgdakalaesKIGSTAIRKW 937  Ustilago maydis...
EAK90683      313 SSIVGGadi-fefDALIATPESYNNAAKINDLRVACGSPVVKLVKVpfvyrplinhsegvkptngssvKFCSTGLRYS 389  Cryptosporidium...
AAQ65117       94 TDPYGPsivdenlEAIVVSKETLPGGLSVNRKRAERGLSQLKIEVVeivsd------------gssgnKISSSTLRKM 159  thale cress
AAT92628      221 RDVCGPtgkvpeiECLVVSRETVSGAETVNKTRIEKGMSPLAVHVVnvlggre---------edgwseKLSSTEIRRL 289  baker's yeast

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