1DS7,1V5Z


Conserved Protein Domain Family
NfsB-like
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cd02149: NfsB-like 
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nitroreductase similar to Escherichia coli NfsB
NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.
Links
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Statistics
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PSSM-Id: 380324
Aligned: 21 rows
Threshold Bit Score: 137.383
Created: 13-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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FMN bindingdimer interface
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN binding site [chemical binding site]
Evidence:
  • Structure:1DS7: an Escherichia coli nitroreductase homodimer of binds 2 molecules of FMN, contacts at 4A
  • Comment:binding involves contacts from two monomers
  • Citation:PMID 9654450
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             ### #                       #####                             #  #      
1DS7_A      2 DIISVALKRHSTKAFDAskkLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASt-eEGKARVAKsaagnyvFNERKMLDASH 80  Escherichia coli
AAD07997    8 KRRQLLNERHSCKMFDShyeFSSTELEEIAEIARLSPSSYNTQPWHFVMVTd-kDLKKQIAAhs----yFNEEMIKSASA 82  Helicobacter pylor...
Q57431      8 QVLELFHQRSSTRYYDPtkkISDEDFECILECGRLSPSSVGSEPWKFLVIQn-kTLREKMKPfs----wGMINQLDNCSH 82  Haemophilus influe...
O34475      7 QILDAYNFRHATKEFDPnkkVSDSDFEFILETGRLSPSSLGLEPWKFVVVQn-pEFREKLREyt----wGAQKQLPTASH 81  Bacillus subtilis
AAK03916    7 DILNAFHFRHACKAYDPskkITEEDFRFILETGRLSPSSFGFEPWKFLVIEn-pDIKALIRDta----wGAKDKVMDCSH 81  Pasteurella multoc...
AAP78370    3 PFLESIYFRHSCKLFDEnkkIPKETFEEILEVGRLAPSSFGMEPTRLLVLRs-dEAKKALRPl-----cWDQPQITSASE 76  Helicobacter hepat...
NP_975349   5 YIKELMLNRKSARDFDLnksISDQDLEIILTSMRMSPSAFNLMNLRLLIIDrncGFKTELSPl-----fYNQLNFINADK 79  Mycoplasma mycoide...
YP_012346   4 KILDAFLFRHAAKDFDAariIPEPVFATILQAGRLSPSSFGFEPWRFLVVQd-aALRAKLHAft----wGGQKQIPNCSH 78  Desulfovibrio vulg...
YP_053530   3 NLAELMTQRRSARDFDVnyeIPEADFNDIIQAMRMSPSSYGILGQRLLVVNr-gEMRQKLVPf-----fYNQLNYVNASK 76  Mesoplasma florum L1
AAZ97684   20 NVLGAIQQRYACRRFVAgrpPSDDELVSLIEAARLAPSAFGLEPWRFLTVId-aAQRAELARa-----cFGQPAAATAPA 93  Thiobacillus denit...

Feature 1                                                                     # ##            
1DS7_A     81 VVVFCAKTamddvwlklvvdqedadgrfatpe---akaandkgrkffadmhrkdlhddaEWMAKQVYLNVGNFLLGVAAL 157 Escherichia coli
AAD07997   83 LMVVCSLRpsellphghymqnlypesykvr--------vipsfaqmlgvrfnhsmqrleSYILEQCYIAVGQICMGVSLM 154 Helicobacter pylor...
Q57431     83 LVVILAKKnarydspffvdvmarkglnaeqqq---aaltkykalqeedmkllendrtlfDWCSKQTYIALANMLTGASAL 159 Haemophilus influe...
O34475     82 FVLILARTakdikynadyikrhlkevkqmpqdvyegylskteefqkndlhllesdrtlfDWASKQTYIALGNMMTAAAQI 161 Bacillus subtilis
AAK03916   82 FVVVLVRQpetlladgeyvqhmmrdvhhipeemrkirqnfyrtfseqdfaltenpkafyDWACRQSYIALANMLTSAAMI 161 Pasteurella multoc...
AAP78370   77 VVVFKSLQsdlvppseyakrntfrrkmdlng-----yetfcsrlgdylkargftdekiaHWSALQAYIMATYMVSYASYI 151 Helicobacter hepat...
NP_975349  80 VILFVSDKtnkilnhtidktvnkmfnetqve-----iankfkknvinatsqlaqmneldNWSKTTAHITAGIATIAAASL 154 Mycoplasma mycoide...
YP_012346  79 LVVYLARKgmdtradsdyvrdfvdrvqalpadvqkmkleryeafqkddfnlwgderalfDWACKQVYIALGNMMTVAALL 158 Desulfovibrio vulg...
YP_053530  77 FIIVLGVNhkglqevtnhsldlrfkgnpegr------styganinknlfggnlsdqqldNYSSQQTYITTGIATAVAASL 150 Mesoplasma florum L1
AAZ97684   94 FITIVALVdalepdsdyvrarfeaearg-----------gpvaplhemyrafyraeaiaAWAQGQCHFAAAHLLLQAAHL 162 Thiobacillus denit...

Feature 1         #####                                                   # #      
1DS7_A    158 GLDAVPIEGFDa----AILDAEFGL-----KEKGYt----SLVVVPVGHHsvedfnatlPKSRLPQNIT 213 Escherichia coli
AAD07997  155 GLDSCIIGGFDp----LKVGEVLEE-----RINKPk----IACLIALGKRvae----asQKSRKSKVDA 206 Helicobacter pylori 26695
Q57431    160 GIDSCPIEGFHy----DKMNECLAEeg-lfDPQEYa----VSVAATFGYRsrd----iaKKSRKGLDEV 215 Haemophilus influenzae
O34475    162 GVDSCPIEGFQyd-hiHRILEEEGL-----LENGSf---dISVMVAFGYRvrd----prPKTRSAVEDV 217 Bacillus subtilis
AAK03916  162 GIDSTPIEGFPl----EKMDQAFVEqg-lyDTKTYk----LSVMIAFGYRlne----pkAKTRQAFDDV 217 Pasteurella multocida subsp. ...
AAP78370  152 KIDTCYIEGFEk----RKVEELYGLd----RFKEQ-----VSLIVCFGYRgka----qqARYRITLDEL 203 Helicobacter hepaticus ATCC 5...
NP_975349 155 NIDSCIIGGFN-----AKVLETFFIqknylSEDEQ-----IVLTMSFGYM---------SKSIKPKSKI 204 Mycoplasma mycoides subsp. my...
YP_012346 159 GVDSCPMEGFQqqridEVLASDFGI-----DQSQYg----VACMAAFGYRvad----prPKTRQALHDV 214 Desulfovibrio vulgaris subsp....
YP_053530 151 NVDTCMIGGFN-----AKGLAEFLI-----QEGLMhdyetPFITMAFGKStki----sgEKLRSELKDF 205 Mesoplasma florum L1
AAZ97684  163 GLGSCPIGGFDa----AALARALRL-----PPGET-----PALVIALGHCad-----tmPKRVRKPRAD 212 Thiobacillus denitrificans AT...

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