1JSC


Conserved Protein Domain Family
TPP_AHAS
?
cd02015: TPP_AHAS 
Click on image for an interactive view with Cn3D
Thiamine pyrophosphate (TPP) family, Acetohydroxyacid synthase (AHAS) subfamily, TPP-binding module; composed of proteins similar to the large catalytic subunit of AHAS. AHAS catalyzes the condensation of two molecules of pyruvate to give the acetohydroxyacid, 2-acetolactate. 2-Acetolactate is the precursor of the branched chain amino acids, valine and leucine. AHAS also catalyzes the condensation of pyruvate and 2-ketobutyrate to form 2-aceto-2-hydroxybutyrate in isoleucine biosynthesis. In addition to requiring TPP and a divalent metal ion as cofactors, AHAS requires FAD.
Links
?
Statistics
?
PSSM-Id: 238973
Aligned: 72 rows
Threshold Bit Score: 239.707
Created: 19-Nov-2004
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
TPP-bindingdimer interface
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:TPP-binding site [chemical binding site]
Evidence:
  • Comment:TPP = thiamine pyrophosphate or thiamine diphosphate
  • Structure:1JSC; Saccharomyces cerevisiae acetohydroxyacid synthase binds TPP and divalent metal ion; contacts at 3.5A
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1                                 ####                       # #                        
1JSC_A      414 IKPQTVIKKLSKVANdtgrhVIVTTGVGQHQMWAAQHWtw-rnPHTFITSGGLGTMGYGLPAAIGAQVAKPe-sLVIDID 491 baker's yeast
NP_228358   366 IKPQYVVEKVNEVFPd---dTVVVADVGQNQMWVAQFYkf-khQRSFLCSGGLGTMGYALPAGIGAKIGAPd-kEVVVFA 440 Thermotoga marit...
AAM75981    364 RMGYVLYGNMFRRAVig--aCSVCCDVGQHQMWSAQYNta-giTARRINSGGLGTMGCGLPFAMSAQTLSHg-lYSLCIT 439 Candidatus Tremb...
NP_718816   374 IAPKLLLEKLSNSLPe---nTIVTTGVGAHQHWVARHFrfalpHRQLFTSAGHGTMGYDLPTAIGAAIHSPe-sTILCIA 449 Shewanella oneid...
BAC57937    357 LTVPWALHQVAQNTKgk--pYAFATDVGQHQMWAALHLri-eePRTWLTSGGLGTMGFGLAAAMGAQMYYGdsrRVIHVA 433 Selenomonas rumi...
YP_203568   370 FSPFQIVNSLSQCFRgk--aVQFVADVGNHQMWLAHSLll-ekQQKSHHSGGLGAMGFSLPTAIGVKLATDd--LVVSIS 444 Vibrio fischeri ...
ABA88370    370 VAPDELLSYIDRATKek--vTTVVTGVGSHQQWAARYFsfdypRKCLLTSAGHGTMGYALPVALGAAKADPd-rLVLCVD 446 Pelobacter carbi...
ZP_00820355 371 ISPFKLFSTLNELAEgq--aLDYVVDVGNNQMWAAHTLrl-gsRQAMHHSGGLGTMGFAIPSAVGACIAGGk--PVVVIT 445 Yersinia bercovi...
YP_437901   360 IQGPDFIATMDKVTPp---eAIISCDVGQHQMWVAQYFdf-nhPRQHLSSGGLGTMGYGLPAAMGAQFAKPg-aPVINVS 434 Hahella chejuens...
YP_110437   371 ISPGVVFARLNEAMAgk--aVHYVCDVGNHQMWAAQGLrl-gaTQAVHYSGGMGAMGFALPTAIGIACSGDgggKTVVIT 447 Burkholderia pse...

Feature 1        ###  #                     #                                                   
1JSC_A      492 GDASFNMTLTELSSAVQAGTPVKILILNNEEQGMVTQWQSLFYehrysh--thqlnPDFIKLAEAMGLKGLRVkkqeELD 569 baker's yeast
NP_228358   441 GDGGFQMNIQELMTIKRYNLPVKIIVMDNKALGMVRQWQQLFFncrysa-tilsdnPDFAKIAEAVGIKAMRIekpdQVD 519 Thermotoga marit...
AAM75981    440 GNGSLQMSMHELPTLRDMGSRVIVIVLNNGSLGMVRQWQQVEHcgrysq-sasamyACSSSIAEQQGHVGVDVlgpvHVF 518 Candidatus Tremb...
NP_718816   450 GDGSFQMNIQELGVIKELGLKIKILVLDNHRLGLVSQFQLMNWetdtac--gnkqsPNFALIAQGYGIKALHCsdsaKLE 527 Shewanella oneid...
BAC57937    434 GDGGIKMTGNEFYTIARLNLPVISLIVNNTSLGMIRQLQKVMYkeryia-cefdyqMDYAAYAGSFGIKAETVstqeEFA 512 Selenomonas rumi...
YP_203568   445 GDGGFQLNVQELDIINREQLPILVIILNNHSLGMVKNFQDMYFegrsqptywngysCSFKDIGEAYGIESHKVndnfDFL 524 Vibrio fischeri ...
ABA88370    447 GDGSFQLNIQELALSAELNLPIKIVLLDNSRLGIVSQFQRITFaddpvt--gdfcnPDFRAIAEAYGVRAFDLe---TFD 521 Pelobacter carbi...
ZP_00820355 446 GDGGAQLNIQELDIIARERLPILTVVLNNHSLGMVRGFQEMYFegrnsstywdgytSQFQALGAAYGIDSRSLsdleTFK 525 Yersinia bercovi...
YP_437901   435 GDGSFMMNMQELATIQRYSLPIKLIILDNQRLGMVRQQQELFYagryse-vdlsdnPDFSAIAKAFGIPALTIskrcQMR 513 Hahella chejuens...
YP_110437   448 GDGSLQVNVQELDTLSRLQLDVAIVVMNNYSLGMVKNFQDMYFdgknistkqgyscPSFVALARAYGIESMTVaapaELQ 527 Burkholderia pse...

Feature 1                                        
1JSC_A      570 AKLKefvs-tkgPVLLEVEVDKKVPVLPMVAGG 601 baker's yeast
NP_228358   520 EAIEklak-skePMLIHAVVDPAENVLPMVPPG 551 Thermotoga maritima MSB8
AAM75981    519 AAFAesrssvrnTALLNVLHSSHECVWPMVQGG 551 Candidatus Tremblaya princeps
NP_718816   528 QTLAefie-tdeSILLHIEIATQHDVLPMLLGG 559 Shewanella oneidensis MR-1
BAC57937    513 EALNrala-dqdHPRVIVLNVWRSFVEPMIKGG 544 Selenomonas ruminantium
YP_203568   525 SNVKsyvd-nprPLLIEVSIENITSCKPRLAFG 556 Vibrio fischeri ES114
ABA88370    522 PAVVdaflqtdgPTLLHARVQHDAPVSPMLLGG 554 Pelobacter carbinolicus DSM 2380
ZP_00820355 526 AAVSefla-sgkPMLLEVLMPDARECRPRLEFG 557 Yersinia bercovieri ATCC 43970
YP_437901   514 RSIEtlla-ypgPMLLHVHIEADTNVWPIVKPG 545 Hahella chejuensis KCTC 2396
YP_110437   528 RAIDrvag-srrPLLVEIAMPEATECRPRLAFG 559 Burkholderia pseudomallei K96243

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap