2YWE,3CB4


Conserved Protein Domain Family
LepA
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cd01890: LepA 
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LepA also known as Elongation Factor 4 (EF4)
LepA (also known as elongation factor 4, EF4) belongs to the GTPase family and exhibits significant homology to the translation factors EF-G and EF-Tu, indicating its possible involvement in translation and association with the ribosome. LepA is ubiquitous in bacteria and eukaryota (e.g. yeast GUF1p), but is missing from archaea. This pattern of phyletic distribution suggests that LepA evolved through a duplication of the EF-G gene in bacteria, followed by early transfer into the eukaryotic lineage, most likely from the promitochondrial endosymbiont. Yeast GUF1p is not essential and mutant cells did not reveal any marked phenotype.
Links
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Statistics
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PSSM-Id: 206677
Aligned: 54 rows
Threshold Bit Score: 294.056
Created: 6-Jan-2012
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:Based on sequence similarity with elongation factor EF-Tu.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1              ######                                                                 
2YWE_A      7 RNFCIIAHVDHGKSTLADRLLEytgaisEREKREQLLDTLDVERERGITVKMQAVRMFYkak----------------dg 70  Aquifex aeolicus
AAC28402  100 RNFSIIAHVDHGKSTLADRLLEltgaiaRNGGQHQVLDNLQVERERGITVKAQTASIFHrh-----------------kg 162 fruit fly
NP_013392  47 RNFSIVAHVDHGKSTLSDRLLEithvidPNARNKQVLDKLEVERERGITIKAQTCSMFYkdk---------------rtg 111 baker's yeast
EAL18811   92 RNLSIIAHIDHGKSTLADRLLQmtg-tvPASSSPQFLDKLKVERERGITVKAQTVSLIHqhk----------------dg 154 Cryptococcus neofo...
CAG31346   60 RNFSIIAHVDHGKSTLADRLLEitgtiaKTDRNKQVLDKLQVERERGITVKAQSASLFYny-----------------kg 122 chicken
BAD68406   58 RNFSIIAHVDHGKSTLADRLLEltgtikKGHGQPQYLDKLQVERERGITVKAQTATMFYrhannqlp------asdqpda 131 Japanese rice
AAW30033   67 RNFSIIAHIDHGKSTLADRLMEltgtikKGHGQPQYLDKLQVERERGITVKAQTATMFYenkve------------dqea 134 thale cress
CAJ07762  132 RNVSVVAHVDHGKTTLSDAMLRfsnllpADGATGTFTDRLKVEKERGITIKAQTCSVLLtvr---------------etg 196 Leishmania major
XP_758038  96 RTFSIISHVDHGKSTLADRLLEltgtipSDGSNQQVLDKLKVERERGITVKSQAVTMVYdydgpregfisafqdgfvprp 175 Ustilago maydis 521
XP_814861  97 RNFAVVAHVDHGKTTLSDAILRrtg-vlSGSQVGTYTDRLLVERERGITIKAQTCSIFVvr-----------------dg 158 Trypanosoma cruzi ...

Feature 1                                                                   ## #              
2YWE_A     71 NTYKLHLIDTPGHVDFSYEVSRALAACEGALLLIDASQGIEAQTVANFWKAVEQDLVIIPVINKIDLPs--ADVDRVKKQ 148 Aquifex aeolicus
AAC28402  163 QLYLLNLIDTPGHVDFSNEVSRSLAACDGVVLLVDACHGVQAQTVANYHLAKQRQLAVVPVLNKIDIKh--ANPDQVCQD 240 fruit fly
NP_013392 112 KNYLLHLIDTPGHVDFRGEVSRSYASCGGAILLVDASQGIQAQTVANFYLAFSLGLKLIPVINKIDLNf--TDVKQVKDQ 189 baker's yeast
EAL18811  155 HKYLINLIDTPGHVDFSYEVSRSLGACEGALLLVDCSQGIQAQTLSVFHHALEADLEMLAVINKVDLPh--AYPEETSEE 232 Cryptococcus neofo...
CAG31346  123 VNYLLNLIDTPGHVDFNYEVSRSLSACQGVLLVVDANEGIQAQTVANFYLAFEAQLAIIPVINKIDLKn--ADPERVEKQ 200 chicken
BAD68406  132 PSYLLNLIDTPGHVDFSYEVSRSLAACQGALLVVDAAQGVQAQTIANFYLAFESNLSIIPVINKIDQPt--ADPDNVKAQ 209 Japanese rice
AAW30033  135 SGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQGVQAQTVANFYLAFEANLTIVPVINKIDQPt--ADPERVKAQ 212 thale cress
CAJ07762  197 TQYLVNLIDTPGHVDFQYEVSRSLCASEGAALLVDVRQGVEAQTMAQFYAALEQNLTILPVLTKMDNVmsdAEVEKTLLQ 276 Leishmania major
XP_758038 176 GRYLLNLIDCPGHVDFSYEVSRSLSACQSALLVVDATQGVQAQSITVFELAKQKNLTIVPVLNKSDLPa--SDPDRCSLQ 253 Ustilago maydis 521
XP_814861 159 EEFLLNLIDTPGHVDFQYEVSRSLSASDAALLLVDAAQGIEAQTMAHFHMALDRGLTILPVLTKMDAVlsdAPVDRALQD 238 Trypanosoma cruzi ...

Feature 1                        ###                    
2YWE_A    149 IEEVLGLDp----EEAILASAKEGIGI-EEILEAIVNRIPPP 185 Aquifex aeolicus
AAC28402  241 LKLLFGIDp----DEVLRVSAKLGTGV-SEVLERVIETVPPP 277 fruit fly
NP_013392 190 IVNNFELPe----EDIIGVSAKTGLNVeELLLPAIIDRIPPP 227 baker's yeast
EAL18811  233 IESSLGLEk----SKHMRISAKSGLGV-EGVLDSIIEGLPAP 269 Cryptococcus neoformans var. neoformans B-3501A
CAG31346  201 IEKLFDIPv----NECIRISAKQGTNI-DKVLEKVIEKIPPP 237 chicken
BAD68406  210 LKRLFDIDp----SEALLTSAKTGQGL-SQVLPAVIERIPSP 246 Japanese rice
AAW30033  213 LKSMFDLDt----EDVLLVSAKTGLGL-EHVLPAVIERIPPP 249 thale cress
CAJ07762  277 LEDSTGLLs----REVILTSAKSKQGI-EQLFQQIIDKVPPP 313 Leishmania major
XP_758038 254 MEEILGIDttlpgQEPLLISAKTGRGV-DSVLRALVERTKPP 294 Ustilago maydis 521
XP_814861 239 LEDSTGLLr----REVLFTSAKEQVGI-EALLHAIIERVPPP 275 Trypanosoma cruzi strain CL Brener

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