1G7T,1G7R,1G7S,3IZY


Conserved Protein Domain Family
IF2_eIF5B
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cd01887: IF2_eIF5B 
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Initiation Factor 2 (IF2)/ eukaryotic Initiation Factor 5B (eIF5B) family
IF2/eIF5B contribute to ribosomal subunit joining and function as GTPases that are maximally activated by the presence of both ribosomal subunits. As seen in other GTPases, IF2/IF5B undergoes conformational changes between its GTP- and GDP-bound states. Eukaryotic IF2/eIF5Bs possess three characteristic segments, including a divergent N-terminal region followed by conserved central and C-terminal segments. This core region is conserved among all known eukaryotic and archaeal IF2/eIF5Bs and eubacterial IF2s.
Links
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Statistics
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PSSM-Id: 206674
Aligned: 102 rows
Threshold Bit Score: 166.108
Created: 6-Jan-2012
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Structure:1G7T: Methanothermobacter thermautotrophicus IF2/eIF5B binds GppNHp, a GTP analog and Mg2+, defined using 3.5 A contacts.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1              #######                                                            #    
1G7T_A       6 PIVSVLGHVDHGKTTLLDHIRGSAVasreagGITQHIGATEIPMdviegicgdflkkfsiretLPGLFFIDTPGHEAFTT 85   Methanothermobac...
CAI36009    49 PVVTVMGHVDHGKTTLLDKLRETQVaamevgGITQHIGAFLVPIil----------------aINKCDKTDADPEKVKKE 112  house mouse
CAJ04974   182 PVFAVMGHVNHGKTALLDALQGSRIgaeephHITQSVRAFTMPRpg---------------adNDLFTFIDTPGHRIFVE 246  Leishmania major
XP_652306  301 PICCVLGHVDTGKTKILDRMRRTNVqrgeagGITQQIGSTFFPLdsiekmtekmkdktklkfkIPGLLVMDTPGHEAFNN 380  Entamoeba histol...
XP_814082  174 PVIAVMGHSQHGKTTLLDVLQKTSFseeesrGTTQSIRAFTISAse---------------ngFRSVTFVDTPGQRVFTE 238  Trypanosoma cruz...
CAD26978    75 PICCILGHVDTGKTKLLDKLRESNVqeeeagGITQQIGATFFPVselfrkc------gkreskLPGILVIDTPGHESFAN 148  Encephalitozoon ...
AAZ41775   556 GVVCVLGHVDTGKTKILDKLRRTHVqdseagGITQQIGATNVPIeaikeqtkyvkaaagfehrLPGLLIIDTPGHESFSN 635  fruit fly
CAF95765   609 PVVCVLGHVDTGKTKILDKLRHTHVqdgeagGITQQIGATNVPKetiveqtkm-vknfkenlkIPGMLIIDTPGHESFSN 687  Tetraodon nigrov...
XP_641984  461 PIICILGHVDTGKTSLLDKIRNTNVqggearGITQQIGASFIPVdaikeqtksfaekikmdfkLPGLLLIDTPGHESFNN 540  Dictyostelium di...
AAW41808   632 PICCILGHVDTGKTKLLDKIRQTSVqegeagGITQQIGATFFPRsaieektevvnkdhaykvqIPGLLIIDTPGHESFTN 711  Cryptococcus neo...

Feature 1                                                           ## ##                      
1G7T_A      86 LRKRGGA----LADLAILIVDINEGf--kPQTQEALNILRm---yRTPFVVAANKIDRihgwrvhegrpfm------etf 150  Methanothermobac...
CAI36009   113 LLAYDVVceeyGGDVQAVHVSALTGdnlmALAEATIALAEilelkADPTGPVEGTVIEsftdkg---------------- 176  house mouse
CAJ04974   247 TRFHVQL----MADVIALVISVAEGi--eSQTHETIKVALn---vDKPVIVVLNKLDLlsdaltaekavrrilaelysig 317  Leishmania major
XP_652306  381 LRSRGTS----LCDIAVLVVDLMHGl--ePQTIESINLLKq---kNTPFVVALNKIDRcyqwketdgmpfk------etf 445  Entamoeba histol...
XP_814082  239 TRFHAQI----IADALLLVVSLVEGv--qIQTFEVIKVALn---iDKPILVVLNKLDLysdprkadeamfnvlmdlreag 309  Trypanosoma cruz...
CAD26978   149 LRTRGSS----LCNLAILVVDIVHGi--eAQTLESIELLRk---rRTPFVVALNKIDRlygwkstpfggik------eil 213  Encephalitozoon ...
AAZ41775   636 LRNRGSS----LCDIAILVVDIMHGl--ePQTIESIQLLKk---kKCPFIVALNKIDRlydwkqlarrdvr------dvl 700  fruit fly
CAF95765   688 LRNRGSS----LCDIAILVVDIMHGl--ePQTLESINLLKe---kKCPFIVALNKIDRlydwkkspdtdiv------atl 752  Tetraodon nigrov...
XP_641984  541 LRSRGSG----LCDLAILVIDIMHGl--qAQTLESINLLRm---rKTPFIVALNKVDRiydwkpcvntdfk------eay 605  Dictyostelium di...
AAW41808   712 LRSRGSS----LCNIAILVVDITHGl--ePQTIESLNLLRq---gRTPFIVALNKIDRmygwkaspnagfr------etl 776  Cryptococcus neo...

Feature 1                                                                             ###      
1G7T_A     151 skqdiqvqqKLDTKVYELVGKLHEegfeser------------------------fdrvtdfASQVSIIPISAITGE-GI 205  Methanothermobac...
CAI36009   177 ---rgpvttAIIQRGTLRKGSILVagkswakv-----------------------rlifdenGKILNEAYPSMPVGIiGW 230  house mouse
CAJ04974   318 ldvhllqreKDVEALATKASTACCssrgrsltdlrpqaeyfapmktvdpeykgskrhpqvqlLRKSYGVCVSAATGA-HI 396  Leishmania major
XP_652306  446 akqnedtkqEFENRLKSTIADFAAqginacl------------------------yydnkdiKEYVSLVPTSAITGE-GI 500  Entamoeba histol...
XP_814082  310 lnvvmvhkpKDIERLRACESNTKLdaqsskevqntlqlfapm--kkvdaqykgskthpvvnlKRSCAGVCISARERE-NL 386  Trypanosoma cruz...
CAD26978   214 kkqskatinDFKKRLDNTVVAFAEmglnakl------------------------fyentdqKRFVSLVPTSAISGE-GI 268  Encephalitozoon ...
AAZ41775   701 keqqsntqlEFQQRTKDVILQFAEqglnaal------------------------fyentdpKTYISLVPTSAISGE-GM 755  fruit fly
CAF95765   753 rkqkkntkdEFDERAKAVIVEFAQqglnaal------------------------fyenrdpRTFVSLVPTSAHSGD-GM 807  Tetraodon nigrov...
XP_641984  606 kiqsksaaqEFDYKVKDIIAALAGqelnael------------------------ywrnkdhRKYVSLVPTSANTGE-GI 660  Dictyostelium di...
AAW41808   777 naqsksvrsEFDDRVAKTKLAFAEqglnaei------------------------fdenknlGRNISLVPTSAVTGE-GI 831  Cryptococcus neo...

Feature 1                    
1G7T_A     206 PELLTMLMGLAQQY 219  Methanothermobacter thermautotrophicus
CAI36009   231 RDLPSAGDEILEVE 244  house mouse
CAJ04974   397 PFLWRVLQLCRDCV 410  Leishmania major
XP_652306  501 PDLVAVLIAMTQRL 514  Entamoeba histolytica HM-1:IMSS
XP_814082  387 GLLWSLIELMSSTL 400  Trypanosoma cruzi strain CL Brener
CAD26978   269 PDLISLILELSEKY 282  Encephalitozoon cuniculi GB-M1
AAZ41775   756 GNLLFMIADFCQNM 769  fruit fly
CAF95765   808 GNLIALLVELTQTM 821  Tetraodon nigroviridis
XP_641984  661 SDLMLVVIQLMQKL 674  Dictyostelium discoideum AX4
AAW41808   832 PDMLMLLVKLTQER 845  Cryptococcus neoformans var. neoformans JEC21

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