1S1M,1VCO,1VCN


Conserved Protein Domain Family
GATase1_CTP_Synthase

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cd01746: GATase1_CTP_Synthase 
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Type 1 glutamine amidotransferase (GATase1) domain found in Cytidine Triphosphate Synthetase
Type 1 glutamine amidotransferase (GATase1) domain found in Cytidine Triphosphate Synthetase (CTP). CTP is involved in pyrimidine ribonucleotide/ribonucleoside metabolism. CTPs produce CTP from UTP and glutamine and regulate intracellular CTP levels through interactions with four ribonucleotide triphosphates. The enzyme exists as a dimer of identical chains that aggregates as a tetramer. CTP is derived form UTP in three separate steps involving two active sites. In one active site, the UTP O4 oxygen is activated by Mg-ATP-dependent phosphorylation, followed by displacement of the resulting 4-phosphate moiety by ammonia. At a separate site, ammonia is generated via rate limiting glutamine hydrolysis (glutaminase) activity. A gated channel that spans between the glutamine hydrolysis and amidoligase active sites provides a path for ammonia diffusion. CTPs belong to the triad family of amidotransferases having a conserved Cys-His-Glu catalytic triad in the glutaminase active site.
Statistics
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PSSM-Id: 153217
View PSSM: cd01746
Aligned: 63 rows
Threshold Bit Score: 230.133
Threshold Setting Gi: 17939058
Created: 27-Aug-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
catalytic triadactive siteputative
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:Conserved trio of residues critical for the catalytic hydrolysis of glutamine and the production of ammonia.
  • Comment:A gated channel that spans between the glutamine hydrolysis and amidoligase active sites provides a path for ammonia diffusion.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1S1M_B    290 VTIGMVGKYiELPDAYKSVIEALKHGglk--nrvSVNIKLIDSQdvetrg---------------veilkgLDAILVPGG 352 Escherichia coli
CAB71268    7 PRLALVGDRgNHDEPSHPKIDAMREQw-------DLTTEWIPTTdihdv-----------------svldgFDGIWVVPG 62  Streptomyces coeli...
1VCO_A    301 VKIAIAGKYvKMPDAYLSLLEALRHAgik--nraRVEVKWVDAEsleaadl--------------eeafrdVSGILVPGG 364 Thermus thermophilus
EAA38943  630 CIIAIVGKFvAREDSYLSILEALRATmhkyyhdkPFAFLWVDPTaldeknc--------------ddilqeAHGFVLPGG 695 Giardia lamblia AT...
EAK88086  347 SRIGIVGKYiASDDTYLSVIEALKHAalh--vkiQLEVVWIEATqleaeteiisnnsalktskstwdllksVNGILVPGG 424 Cryptosporidium pa...
Q8SQI7    299 VRIGIVGKYaPEFDSYTSLVNALKFSgah--igvNVEIVWINSEsysvc------------------dferCDGVVIPGG 358 Encephalitozoon cu...
O51522    296 INIAICGKYaELDDSYASIRESLVHVaah--ldlLIKSTLIDSNdlnes------------------clkeFDGIIVPGG 355 Lyme disease spiro...
CAI74140  302 VTVGIVGKYsASTDSYLSVLNALKHSsle--cnlKLNIKWVDYSkfedydse-----------kfkshfkdLDGVLIPGG 368 Theileria annulata
AAG05656    9 LRIALVGDHdPHITAHRAIPLALRLAgea--lglEIAFDWLASDrlpae-----------------palerYDGFWCVPG 69  Pseudomonas aerugi...
YP_016105 297 IKLAMLGKYtEFPDAYKSIVEALFVSsty--envKVVLTWLSSEnikedni--------------eltlgkFDGVVILPG 360 Mycoplasma mobile ...
Feature 1                               #                                                     
1S1M_B    353 FGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARHVANMENANSTEFVPDCKYPVValitewrdeng-------- 424 Escherichia coli
CAB71268   63 APYFNQKGVHVAVRHARENGLPFLGTCGGFFSALIEHAQNVLHLPEAAGVDEDPEKLMPLVtplkcsfr----------- 131 Streptomyces coeli...
1VCO_A    365 FGVRGIEGKVRAAQYARERKIPYLGICLGLQIAVIEFARNVAGLKGANSTEFDPHTPHPVIdlmpeq------------- 431 Thermus thermophilus
EAA38943  696 FGDIGVNGLMLSIKYARTHNKPILGLCLGFQMMVCEFARNVCGLSHANSTEMDPDTPHKLIipisemletgqikp----- 770 Giardia lamblia AT...
EAK88086  425 FGKRGINGKIEAINYSRVNNIPFLGICLGMQLSVVEFCKNVLKLPLATSQEFEDDLVLDLKpissmdelnlahasngtls 504 Cryptosporidium pa...
Q8SQI7    359 FGARGISGKIEAIRHARENGVPLLGICLGYQLSVIEMCRNILGMSDAFSEEFQPSGKNLVVrfisd-------------- 424 Encephalitozoon cu...
O51522    356 FGGKGYEGKIMAIKYARENNIPFLGICLGLQLAVIEFARNVCGILDADTEENLARDKPLKSpvihllp------------ 423 Lyme disease spiro...
CAI74140  369 FGTRGFMEKKMAVRYCRLNKVPFLGICLALQLTVVDVAEEFEPKACHGEDSKTEEKYHVVTlmpef-------------- 434 Theileria annulata
AAG05656   70 SPYRDADAVLRLIAHARGRRRPFLGTCAGFQHTILEFARNALGWQAATHGEEHPHSDQAVIaalpcall----------- 138 Pseudomonas aerugi...
YP_016105 361 FGIRGWHGKIVASSWLKKVNIPTFGICLGFQAMVIAQAREKGIKDADSAEFSKKGTMVLNTiqest-------------- 426 Mycoplasma mobile ...
Feature 1                                                                                     
1S1M_B    425 ----------------------nvevrseksdLGGTMRLGAQqcqlvddslv------------------rqlynaptiV 464 Escherichia coli
CAB71268  132 -------------------------gekapltVMENSRLAGIygt-------------------------------tevE 155 Streptomyces coeli...
1VCO_A    432 ---------------------------levegLGGTMRLGDWpmrikpgtll------------------hrlygkeevL 466 Thermus thermophilus
EAA38943  771 ------------------lhkqqnaanpgttaKYSTMRLGDLlvsvksntml-----------------secynkypqvK 815 Giardia lamblia AT...
EAK88086  505 vgeeilspntdielssrsvhvvvtmseftssdKGGTMRLGGWntmihdknslayriynsckfqsfttlctetgeeiytiR 584 Cryptosporidium pa...
Q8SQI7    425 ----------------------------engvVDKRLRVGGYgvelrdglvk------------------klyggvetvR 458 Encephalitozoon cu...
O51522    424 -------------------------eqkgikdKGATMRLGGYpvilkkntia------------------fklygqdriI 460 Lyme disease spiro...
CAI74140  435 ---------------------------sgkdkKGGTMRLGALetklvkgtli-----------------yslydkkdtiV 470 Theileria annulata
AAG05656  139 ------------------------earedvrlLRGSRLALAYaad--------------------------------wiE 162 Pseudomonas aerugi...
YP_016105 427 ----------------------------kgssLGGTLRLGEYdvnvekntla------------------fdiykstkiS 460 Mycoplasma mobile ...
Feature 1                                                            # #                
1S1M_B    465 ERHRHRYEVNNMLLKQIEDAGLRVAGrsg-----ddqLVEIIEVPNHPWFVACQFHPEFTSTPrdGHPLFAGFV 533 Escherichia coli
CAB71268  156 EIFHCDYGLTDTFMNAANQGSLRLSAwds------dgAPRALEITGHPFFLGSLFQPELSSAPgsEHKILKAFL 223 Streptomyces coelicolor ...
1VCO_A    467 ERHRHRYEVNPLYVDGLERAGLVVSAttpgmrgrgagLVEAIELKDHPFFLGLQSHPEFKSRPmrPSPPFVGFV 540 Thermus thermophilus
EAA38943  816 ERHWHSFAVNTQYIDTLTEHGLIISGmdft---nqlpMAVELPTEAHPFFMATQYHPEFTSKHrlPHTLFVAFV 886 Giardia lamblia ATCC 50803
EAK88086  585 ERHRHRYEINPEFVPLMEKNGMKFVGkdv-----dvsKNEILELPSHPFFIAAQYHPEFTSRPmsPNPLFYGLI 653 Cryptosporidium parvum
Q8SQI7    459 ERHRHRFEVAQEKVRGLLQHGVRFVGfss-----ggkKINVFEVESHPFFVGVQFHPEFNARPdrPHPLITGLV 527 Encephalitozoon cuniculi
O51522    461 ERFRHRYEVNNDYIDLFAKNGLIVSGfss-----dfkMAKLIEIPENKFFVACQFHPELITRIenPAKLFLGLI 529 Lyme disease spirochete
CAI74140  471 ERHRHRYQVNPAYEERLTNHGVVFSGrdp-----tqgRVSVVELKNHPFFLCTQFHPEYKSTPitPSPPYLGFI 539 Theileria annulata
AAG05656  163 ADYHCRYAIAPRFAAELTGGALRASAwsa------dgAIRAVELEQHPFFVATLFQPERAALAgvLPPLPKAFV 230 Pseudomonas aeruginosa PAO1
YP_016105 461 ERHRHRYEVQPEFQKILSDDEFIFSGfnk-----klnTPEICEVKNLDFYLGTQYHPEFNSTPlePQKLFSAFI 529 Mycoplasma mobile 163K

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