1JDB,1KEE


Conserved Protein Domain Family
GATase1_CPSase

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cd01744: GATase1_CPSase 
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Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II
This group of sequences represents the small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II. CPSase II catalyzes the production of carbomyl phosphate (CP) from bicarbonate, glutamine and two molecules of MgATP. The reaction is believed to proceed by a series of four biochemical reactions involving a minimum of three discrete highly reactive intermediates. The synthesis of CP is critical for the initiation of two separate biosynthetic pathways. In one CP is coupled to aspartate, its carbon and nitrogen nuclei ultimately incorporated into the aromatic moieties of pyrimidine nucleotides. In the second pathway CP is condensed with ornithine at the start of the urea cycle and is utilized for the detoxification of ammonia and biosynthesis of arginine. CPSases may be encoded by one or by several genes, depending on the species. The E.coli enzyme is a heterodimer consisting of two polypeptide chains referred to as the small and large subunit. Ammonia an intermediate during the biosynthesis of carbomyl phosphate produced by the hydrolysis of glutamine in the small subunit of the enzyme is delivered via a molecular tunnel between the remotely located carboxyphosphate active site in the large subunit. CPSase IIs belong to the triad family of amidotransferases having a conserved Cys-His-Glu catalytic triad in the glutaminase active site. This group also contains the sequence from the mammalian urea cycle form which has lost the active site Cys, resulting in an ammonia-dependent form, CPSase I.
Statistics
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PSSM-Id: 153215
View PSSM: cd01744
Aligned: 78 rows
Threshold Bit Score: 204.652
Threshold Setting Gi: 42519110
Created: 27-Aug-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
catalytic sitesubunit
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:Conserved trio of residues critical for the catalytic hydrolysis of glutamine and the production of ammonia.
  • Citation:PMID 9174345

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     # 
1JDB_C      194 VVAYDFGAKRNILRMLVDRGCRLTIVPaqtsaedvlkmnPDGIFLSNGPGDPapcdyAITAIQKFLETd---IPVFGICL 270 Escherichia coli
ZP_00387417 170 IAIIDLGLKHSMLRELSLRQVNANVLPynatvrdiktlrPDGIIISNGPSQAk---eVLPYLRQILDEylgkLPILGVGL 246 Lactobacillus de...
ZP_00062784 170 IVVIDFGLKHGILRMLGDYDANVSVLPyttsvdevltldPDGIILSTGPGDPrslpeSVLTLIRVLQTk---APLLGIGL 246 Leuconostoc mese...
NP_785349   171 VVVIDFGLKHGILRELSKRACNVTVLPytatteeilnldPDGVILSTGPGKPqdlpvSVTEMIKNIQNr---VPLFAIGL 247 Lactobacillus pl...
O08317      167 VAILNFGEKAAITAELQARGADVVVLPptaslkavaayhPDGILLSNGPGDPtdyhtYLATIRQLAQR----YPLAGICL 242 Lactobacillus pl...
NP_965040   170 VAVIDLGLKHSLLRSLSLREINSVVLPynasiydiinlrADAIVISNGPSGVeevapFLKPVFDKFYGk---LPILGIGL 246 Lactobacillus jo...
YP_075032   172 VVAVDFGAKENILRELTARGCDVTVVPatasaeevlslrPEGVVLTNGPGAPtdvpeAVEMVRGLLSQg--dLPIFGICL 249 Symbiobacterium ...
ZP_00663008 174 IVLLDLGTKKGVIDSLVRRDCEVIAVPantgareilrlhPDGILVSDGPGDPgaigyAIDTCRELLGK----VALLGVGL 249 Syntrophomonas w...
YP_360332   175 VVLVDYGAKNSILQSLLKRGAEVFVVPplmpaeqilalaPQGVVLSNGPGDPadlqeSIFIIRQLIGK----LPILGICL 250 Carboxydothermus...
ZP_00783445 173 IVVLDFGLKHSILRELSKRQCDVTVIPyntslegiknlyPEGIILSNGPGNPeklqeILNTIKELQKS----VPMLGIGL 248 Streptococcus ag...
Feature 1                                                                                       
1JDB_C      271 GHQLLALASGAKTVKMKFGHHGGNHPVkdvekNVVMITAQNHGFAVdeatlpa-nlrvthkSLFDGTLQGIHRTDKPAFS 349 Escherichia coli
ZP_00387417 247 GFLAISAYLDLSLVDLPQYQNGTNFPVirqssGQIWQTAMNIGQLVlpddtav-tfneeyfDLHNELLAGFSNDKLQLLA 325 Lactobacillus de...
ZP_00062784 247 GHELFALANDATLVALPSEHHGMNHPIqeiisRQIFYAMQGQGYAVdeelldhkklfvtyrDLVDGAVQGLRHRDYPAFS 326 Leuconostoc mese...
NP_785349   248 GHELFAMANGAQIMKLSPEHHGANHPIrevitNQIIYAAQGQGFAVdadtvdrnklittfvDLIDGTIQGLRLRDFPAFS 327 Lactobacillus pl...
O08317      243 GHQLIALAYGAQTYQLSFGHHGLNHPVqacadGRIIMTSQNHDYAVdpasikgtplivthtELNDGSIEGLRLPHQAVMS 322 Lactobacillus pl...
NP_965040   247 GFLAISNYLDLELLDLIPAYNGSNFPVieqtnNRIWQTAMNIDQLVvpdslsl-nlsrkyfDLRSELLAGYSIKKDKVLA 325 Lactobacillus jo...
YP_075032   250 GHQIAALALGATTYKLPYGHRGANHPVkelatGRIHITSQNHGYAVaaeslpp-evevthvSLHDGTVEGLAHRRLPLFT 328 Symbiobacterium ...
ZP_00663008 250 GHQILALAMGASILRLPYGHRGSNHPVknlenNRVYITTQNHGFSLeesslsgtgievtmrSLNDGSIEGIRHREMPVFS 329 Syntrophomonas w...
YP_360332   251 GHQLLALAHGAQTYKLKFGHRGSNHPVietesKRVFITSHNHGFAVreesligtgleiwfrNLNDNTIEGLRSRYSKILS 330 Carboxydothermus...
ZP_00783445 249 GHQLIAMANGAEIMRLPVAKKGTNYPMrdiatGRLETVSQFNHFTVnrlnlph-dllvtheGLNDQEIVALRHRSFPVMS 327 Streptococcus ag...
Feature 1          # #                
1JDB_C      350 FQGHPEAsPGPHDAAPLFDHFI 371 Escherichia coli
ZP_00387417 326 VAFNPEGaPGTEDAQVIFDDFL 347 Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365
ZP_00062784 327 VQFFPDAaPGPYEATAVFADFF 348 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
NP_785349   328 VQFFPDGaPGPTETRDIFDEFV 349 Lactobacillus plantarum WCFS1
O08317      323 VQFHPEAhPGPQEAGQFFDDFL 344 Lactobacillus plantarum
NP_965040   326 TAFNPEGsPGNFDAASIYDQFL 347 Lactobacillus johnsonii NCC 533
YP_075032   329 VQYHPEAcPGPRENRYLFDRFL 350 Symbiobacterium thermophilum IAM 14863
ZP_00663008 330 LQFDPEGyPGYSETGFFYDDFI 351 Syntrophomonas wolfei str. Goettingen
YP_360332   331 VQFHPEAaPGPNDADFIFDLFL 352 Carboxydothermus hydrogenoformans Z-2901
ZP_00783445 328 VQFYPEAaPGPHDVTYFFDEFL 349 Streptococcus agalactiae H36B

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