1I7Q,1QDL,1I1Q


Conserved Protein Domain Family
GATase1_Anthranilate_Synthase

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cd01743: GATase1_Anthranilate_Synthase 
Click on image for an interactive view with Cn3D
Type 1 glutamine amidotransferase (GATase1) domain found in Anthranilate synthase
Type 1 glutamine amidotransferase (GATase1) domain found in Anthranilate synthase (ASase). This group contains proteins similar to para-aminobenzoate (PABA) synthase and ASase. These enzymes catalyze similar reactions and produce similar products, PABA and ortho-aminobenzoate (anthranilate). Each enzyme is composed of non-identical subunits: a glutamine amidotransferase subunit (component II) and a subunit that produces an aminobenzoate products (component I). ASase catalyses the synthesis of anthranilate from chorismate and glutamine and is a tetrameric protein comprising two copies each of components I and II. Component II of ASase belongs to the family of triad GTases which hydrolyze glutamine and transfer nascent ammonia between the active sites. In some bacteria, such as Escherichia coli, component II can be much larger than in other organisms, due to the presence of phosphoribosyl-anthranilate transferase (PRTase) activity. PRTase catalyses the second step in tryptophan biosynthesis and results in the addition of 5-phosphoribosyl-1-pyrophosphate to anthranilate to create N-5'-phosphoribosyl-anthranilate. In E.coli, the first step in the conversion of chorismate to PABA involves two proteins: PabA and PabB which co-operate to transfer the amide nitrogen of glutamine to chorismate forming 4-amino-4 deoxychorismate (ADC). PabA acts as a glutamine amidotransferase, supplying an amino group to PabB, which carries out the amination reaction. A third protein PabC then mediates elimination of pyruvate and aromatization to give PABA. Several organisms have bipartite proteins containing fused domains homologous to PabA and PabB commonly called PABA synthases. These hybrid PABA synthases may produce ADC and not PABA.
Statistics
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PSSM-Id: 153214
View PSSM: cd01743
Aligned: 130 rows
Threshold Bit Score: 108.391
Threshold Setting Gi: 68230483
Created: 27-Aug-2004
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
catalytic triadglutamine
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:Conserved trio of residues critical for the catalytic hydrolysis of glutamine and the production of ammonia.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1I7Q_D        4 ILLLDNVDSFTYNLvdQLRAS-------------------GHQVVIYRNQIgaeviie--rlqhmeQPVLMLSPGPGTps 62   Serratia marces...
EAL23210      9 TLVLDYYDSYTNNLvtLLTRTyad-------------advLEKLVVVKADEytwdefq--rlvlpnIDCVILSPGPGRpd 73   Cryptococcus ne...
AAN40892    440 ILILDGNDAFTAMFktLFSSL-------------------GALAHVEKVCSgid---------lqgWDLVVAGPGPGNpl 491  Pantoea agglome...
YP_050792   439 ALLVDNEDAFTSMMeyQLRAM-------------------GLSVRLVHYSSsfr---------iepNELLIIGPGPGDpr 490  Erwinia carotov...
Q51519      438 VLIVDAEDTFTSMIakQLRAL-------------------GLVVTVCSFSDeys---------fegYDLVIMGPGPGNps 489  Pseudomonas chl...
EAK84907      8 ILIVDHFDSYTLNLlsLLHPNytlssqlpssqvneltrriSNRVVVLPHTHpllastafhelvrphIDAIILSPGPGSpd 87   Ustilago maydis...
ZP_00568221 496 LLIVDAEDTFTAMGaaQLGAL-------------------GFDVTVRRFDEpyq---------iegVDLVVLGPGPGDpl 547  Frankia sp. EAN...
ZP_00659393 471 VVILDGEDDFVNMLrhVLGVL-------------------GMTSEVVRHADyapg-------cldgYDLVIVGPGPGDpr 524  Nocardioides sp...
ZP_00569666 453 ALVVDGEDTFTGMLahQLRAL-------------------GLTVTVRPWHSagd---------tarFDLVVPGPGPGDpt 504  Frankia sp. EAN...
ZP_00379381 427 CLIVTAEDDFTHMLavLLRSM-------------------GFYVDEQPALDvdel--------padRDLILFGPGPGNpe 479  Brevibacterium ...
Feature 1                                       #                                               
1I7Q_D       63 eag------cmpeLLQRLRGq-----LPIIGICLGHQAIVEAYGGQVGQAGEILHGKASAIahdgegmf----------- 120  Serratia marces...
EAL23210     74 npa------digfALQLLRLh----pLPILGVCLGHQAIGVAFGGKIINTPKITHGHVIPVapvqppiglfas----plw 139  Cryptococcus ne...
AAN40892    492 dvndfrvnamreaIIKMRATg-----QPFFAVCLSHQLLCLQLGLPVARLSPPNQGVQKEIlldr--------------- 551  Pantoea agglome...
YP_050792   491 dlsdvrvirsrklMLNAMRQc-----HPFIAVCFGHQILCASLGYPIAALDYPNQGIQKEIsffg--------------- 550  Erwinia carotov...
Q51519      490 evqqpkinhlhvaIRSLLSQq-----RPFLAVCLSHQVLSLCLGLELQRKAIPNQGVQKQIdlfg--------------- 549  Pseudomonas chl...
EAK84907     88 ka-------adfgAAETLLKdkl-lnLPVLGVCLGHQGLAVAFGGQVTRAASVRHGLQSKLqisassetaaieshtyrsi 159  Ustilago maydis...
ZP_00568221 548 dga-----dpkiaSLRAVTRsllnldVPFLSVCLSHQVLCDLLGLPVIRMAVPNQGVRRTVdlfg--------------- 607  Frankia sp. EAN...
ZP_00659393 525 dgadpkmaslraaVAELMTAg-----RPFLAVCLGHQALCHHLGLPLTYKDIVFQGTQSPVpirg--------------- 584  Nocardioides sp...
ZP_00569666 505 vradprmaamrgvVADLLARr-----RPMLAICLGHQLLAGLLGLRLRRREATYQGLAREIdlfg--------------- 564  Frankia sp. EAN...
ZP_00379381 480 nktdqriaslhrlIRSALLQr-----RPFLAECLSHQILALELGLPIARLAQPHQGEQIELplwn--------------- 539  Brevibacterium ...
Feature 1                                                                                       
1I7Q_D      121 -agmanplPVARYHSLVGsnipad-----------------------------ltvnaRFGEMVMAVRDDrr-------- 162  Serratia marces...
EAL23210    140 kaggetqfDVVVYNSLTVdpmtlpqdle---------------------vtawsipspDRPASIQGLRHRly-------- 190  Cryptococcus ne...
AAN40892    552 -----nleTAGFYNTFCAsinavthsqm--------------------rqrgievysdPDNGNVHALRAHs--------- 597  Pantoea agglome...
YP_050792   551 -----rveKVGFYNTFTAiddhnvppgf---------------------qgeirvsrdEITGHIFGLRGPr--------- 595  Erwinia carotov...
Q51519      550 -----nveRVGFYNTFAAqsssdrldid--------------------gigtveisrdSETGEVHALRGPs--------- 595  Pseudomonas chl...
EAK84907    160 lhgvpqdaLVVRYNSLVVdpdtlpdclqvtawsydqissapaprpgttrkrstishlqTPSEHVAPLRHRd--------- 230  Ustilago maydis...
ZP_00568221 608 -----qlePVYFYNTFVAihpsdlleggp-------------------gvgpvevardLTTGEVHALRGAg--------- 654  Frankia sp. EAN...
ZP_00659393 585 -----rteRVGFYNTFVGrldpgsgap-----------------------egveveadVQTGDIHLLRGPh--------- 627  Nocardioides sp...
ZP_00569666 565 -----drrRVGFYSTFTAefdqdeiltphgvvql--------ardpagvrdpagnsddRRDEAVHALRGPt--------- 622  Frankia sp. EAN...
ZP_00379381 540 -----rqaTLGFYNTYTArsptp-------------------------------fihsNPAGHVRAILHPdsedvavlsg 583  Brevibacterium ...
Feature 1               # #                   
1I7Q_D      163 -rVCGFQFHPESILTTh----GARLLEQTL 187  Serratia marcescens
EAL23210    191 -pIWGVQYHPESISSTc----GSSLLVSFL 215  Cryptococcus neoformans var. neoformans B-3501A
AAN40892    598 --FSSVQFHLESVLTLn----GQALLERFV 621  Pantoea agglomerans
YP_050792   596 --FSSMQFHPESVLTLd----GPRILYESI 619  Erwinia carotovora subsp. atroseptica SCRI1043
Q51519      596 --FASMQFHAESLLTQe----GPRIIADLL 619  Pseudomonas chlororaphis
EAK84907    231 --LTSLSRSPSTIPFSsr--hGSERLSEVG 256  Ustilago maydis 521
ZP_00568221 655 --FASMQFHAASVMTRg----GLTLMDTLV 678  Frankia sp. EAN1pec
ZP_00659393 628 --YRGIQFHAESILTEh----GYDLLHEIV 651  Nocardioides sp. JS614
ZP_00569666 623 --FAGLQFHPESVLSLd----GHEVLRDLL 646  Frankia sp. EAN1pec
ZP_00379381 584 taFASTQFHIESVLTHnndwlLAELIEGVL 613  Brevibacterium linens BL2

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