1T3T


Conserved Protein Domain Family
GATase1_FGAR_AT

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cd01740: GATase1_FGAR_AT 
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Type 1 glutamine amidotransferase (GATase1)-like domain found in Formylglycinamide ribonucleotide amidotransferase
Type 1 glutamine amidotransferase (GATase1)-like domain found in Formylglycinamide ribonucleotide amidotransferase (FGAR-AT). FGAR-AT catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to formylglycinamidine ribonucleotide (FGAM), ADP, Pi, and glutamate in the fourth step of the purine biosynthetic pathway. FGAR-AT is a glutamine amidotransferase. Glutamine amidotransferase activity catalyses the transfer of ammonia from the amide side chain of glutamine to an acceptor substrate. FGAR-AT belongs to the triad family of amidotransferases having a conserved Cys-His-Glu catalytic triad in the glutaminase active site
Statistics
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PSSM-Id: 153211
View PSSM: cd01740
Aligned: 69 rows
Threshold Bit Score: 256.003
Threshold Setting Gi: 56417041
Created: 21-Sep-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
catalytic triadputative active
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:
  • Citation:PMID 8208731
  • Comment:Conserved trio of residues critical for the catalytic hydrolysis of glutamine and the production of ammonia.
  • Comment:The opening of the active site of the glutaminase domain is directed towards the opening of the FGAM synthetase active site. Ammonia passes from the glutaminase domain to the FGAM synthetase domain.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
1T3T_A      1050 VAVLREQGVNShveMAAAFHRaGFDAIDVHmsdllgg------riglgnFHALVACGGFSYGDVLGAGEGWAksilfn-- 1121 Salmonella typ...
YP_010841      6 TLVITGYGTNCeveSAHAARMaGADRVDIAhfsdlva-----grvtladYNFLVFPGGFLDGDDLGSALAAAqrwrhahd 80   Desulfovibrio ...
NP_868461     22 VLVLRAPGTNCdveTAHAFELaGAVAERVHvgrlmen------aalasrYQILCLPGGFSYGDDIAAGRILAtrmrr--- 92   Pirellula sp. 1
NP_987298      8 VLVMSGYGINCeteTAHAFQKaGAETDIVHindliag------kkkmadYEIIMFPGGFSYGDDTGSGNAFAnkikn--- 78   Methanococcus ...
YP_181124      6 TLILRTPGTNCdieTSYAFELaGSETEMVHinellak------psrlaeFQIMAFPGGFGYGDDLGAGRVLAnevrl--- 76   Dehalococcoide...
YP_154115      3 LVVLSGYGLNCeeeTLFAFMEaGRFLNASVsgrvmhinelllnpkclreFNTMVVPGGFSYGDDTGAGNAFAlrmlh--- 79   Anaplasma marg...
ZP_00533163  342 AAIIREKGVNGdreMAYAMYLaGFDVKDVHmtdliag------redlsdVNFIVFVGGFSNSDVLGSAKGWAgaflyn-- 413  Chlorobium pha...
ZP_00667398 1031 ALVLTGFGLNCdleTAYALTIvGADVERVHlnslaqr------ekrladYRIFVVGGGFSWGDDHGAGVIMGmrlkh--- 1101 Syntrophobacte...
CAG35433    1007 IAILRDEGSNSdreMTAAFYSaGFEAWDVCmndllag------svdladFRGLAAVGGFSYADVPESAKGWAatiqfn-- 1078 Desulfotalea p...
NP_904615    984 AAIIRDKGTNGereMAYALYLaGFDVKDVHltdltsg------retledIRMIVFCGGFSNSDVLGSAKGWAggilfn-- 1055 Porphyromonas ...
Feature 1                                #                                                       
1T3T_A      1122 --hrvrdeFETFFHRP-QTLALGVXNGCQMMSNLRELIPGSELWPRFVRNhsdrfe----------------arfslvEV 1182 Salmonella typ...
YP_010841     81 aqgnrlleALRDFVTR-GGLVLGICNGFQLLVKLGLLPALDGQRFDRQVSlshndsar-------------yedrwvhLR 146  Desulfovibrio ...
NP_868461     93 ----hlgdLMHHFVDSgDNLVLGICNGMQVLMRLGVLTSGVGQLQTAGGPaesaddsvppatltwnnhgrfedrwvnlAV 168  Pirellula sp. 1
NP_987298     79 ----nlfdDLTEFINS-GKLILGICNGFQVMTNLGLFALPSTDYGERISAlesntnn--------------ryecrwvHI 139  Methanococcus ...
YP_181124     77 ----klgeKINRFHES-GGLIIGICNGFQALVKTGILPGPMKDGQKITLTennsgrf---------------ecrwtyLA 136  Dehalococcoide...
YP_154115     80 ----glgeEIQAFVEQ-DKLLLGICNGCQILLRVFFPDIALVRNDVGRYQcrw-----------------------vkVK 131  Anaplasma marg...
ZP_00533163  414 --ekaalaLANFYARK-DTLSLGVCNGCQLMTELNLIYPKHPESVKMLHNssgkfe----------------sgfvnlNI 474  Chlorobium pha...
ZP_00667398 1102 ----rlgdDLQAFVAS-GGIVIGICNGFQVLVNLGLLPGFEPGVLKREVAlinndcgn-------------frdqwvnLI 1163 Syntrophobacte...
CAG35433    1079 --prlkemFDTFYNRP-DTFTFGICNGCQLFSLLGWVPQLGTSAEDQPRFagnlsgrf-------------esrwttvKV 1142 Desulfotalea p...
NP_904615   1056 --eqakatLDRFFARP-DTLSLGICNGCQLMAELELLYPEHELKHKLVHNesqkfe----------------stfvtlEI 1116 Porphyromonas ...
Feature 1                                                                                        
1T3T_A      1183 TQSPSLLLqgmvGSQMPIAVSHGEGRVEVrddahlaaleskglva---lryvdnfgkvtetypaNPNGSPNGITAVTTEN 1259 Salmonella typ...
YP_010841    147 VNAASPCVftkgIEHLYVPVRHGEGKLVArdeevmnrlvsgnlval--qyadpetmeptmeypwNPNGSPLGIAGLTDPT 224  Desulfovibrio ...
NP_868461    169 DKTPCVFLr--dIEQMYLPMAHAEGKFIArdaevleelrsagrlalrycepsgeiqsetlpfpiNPNGGDANVAGVCDAS 246  Pirellula sp. 1
NP_987298    140 KENDSVCVftkgINVTHVPIAHGEGRFYCdektyhelkenkqivf---sycdsegnpangeyplNPNGAYQDIAGICDKT 216  Methanococcus ...
YP_181124    137 VNPFSTCVftqgIDRLYLPVAHGEGKLLGspevinklnsvvyy--------tdkdgkrnspypaNPAGTLMDIAGIADES 208  Dehalococcoide...
YP_154115    132 SCCSSIWLk--gIDEMYIPVAHGEGRFYApdnallglaergsvam---kytrpdgeladgqfphNPNGSAYDIAALSGNN 206  Anaplasma marg...
ZP_00533163  475 PENDSVMLhslaGTRLGAWVAHGEGKFSFpyfsdkyqlv---------------mkysydlypgNPNGSDWSTAAICSND 539  Chlorobium pha...
ZP_00667398 1164 GNAASPCVltegIEAIELPVRHGEGKFFAetdvlerlerrqqvil---ryagadgkvaggafpaNPNGSVADIAGICDPT 1240 Syntrophobacte...
CAG35433    1143 QASTSIMLqgmeDLVFGIHVDHGEGRLLFpnadimehvkehgmtp---mvyvddegeateqyplNPNGSAEGLAGLCSAD 1219 Desulfotalea p...
NP_904615   1117 PHNDSIMLgnlaGSKIGVWCAHGEGRFSLpyeekayhvv---------------akyeydgypaNPNGSPHRIAGLCSAD 1181 Porphyromonas ...
Feature 1                # #                                        
1T3T_A      1260 GRVTIMMPHPERVFrtvansw-----------hpenwgEDSPWMRIFRNAR 1299 Salmonella typhimurium
YP_010841    225 GHILGLMPHPEAFNhptnhp------------grgrdeTAPLGTVIFDNAV 263  Desulfovibrio vulgaris subsp. vulgaris str....
NP_868461    247 GRVFGLMPHPERHIdatqhpfw---------trrkeqpEHGEGLQMFRNAV 288  Pirellula sp. 1
NP_987298    217 GRIFGLMPHPERSLysisepeyqlkkeiakrngdiipeFIENNLQIFKNAV 267  Methanococcus maripaludis S2
YP_181124    209 GRIFALMPHPERFVrgsqhpr----------wpaeglkEEGDGLKIFTNAV 249  Dehalococcoides ethenogenes 195
YP_154115    207 GRALLMMPHPERAVfftqrydwtdik-danvragkpvpTYADGFEVFCNAV 256  Anaplasma marginale str. St. Maries
ZP_00533163  540 GRHLAMMPHLERAFmpwqwpi----------ypegrqdEVSPWMVAFVNAR 580  Chlorobium phaeobacteroides BS1
ZP_00667398 1241 GRVAGLMPHPEAFNdwtnhpewtrkr-elarrsgtvapREGEGLKLFRNAV 1290 Syntrophobacter fumaroxidans MPOB
CAG35433    1220 GRHLALMPHPERCFlpwqmhyl---------peemkelEVSPWFQMFKNAY 1261 Desulfotalea psychrophila LSv54
NP_904615   1182 GRHLTMMPHPERSIfpwqcgyy--------pesqrhehQVTPWMQAFRNAY 1224 Porphyromonas gingivalis W83

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