2ALZ,1ZET


Conserved Protein Domain Family
PolY_Pol_iota

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cd01703: PolY_Pol_iota 
Click on image for an interactive view with Cn3D
DNA Polymerase iota
Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Statistics
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PSSM-Id: 176457
View PSSM: cd01703
Aligned: 8 rows
Threshold Bit Score: 494.296
Threshold Setting Gi: 15291835
Created: 6-Mar-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active siteDNA binding
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           ###  ##                        ##  #  #     #                                
2ALZ_A         7 VHVDLDc-FYAQVEMISNPELKDKPLGVQQKylVVTCNYEARKLGvKKLMNVRDAKEKCPQlvlvnGEDLTRYREMSYKV 85  human
1ZET_A         5 VHVDLDc-FYAQVEMISNPELKDKPLGVQQKylVVTCNYEARKLGvKKLMNVRDAKEKCPQlvlvnGEDLTRYREMSYKV 83  human
NP_001017834  40 LHFDLDc-FYAQVEMIRNPALRTKPLGIQQKyiIVTSNYVARELGvNKLMSVTDAVEKCPQlvlvkGEDLTHYREMSYKV 118 zebrafish
XP_368155     49 TNGDDEriILHFVVENKEPSLKSLPVGIKQKsiLATCNYVARRRGvKKLMRISEARDICPDlvlvdGEDLTPFRDTSKIL 128 Magnaporthe gri...
CAD70389      46 CLSDLDc-FYAQVYENKTPSLKSLPLGIRQKslLATCNYPARRLGvKKLMSIASALAICPDlvivdGEDLTPFRDVSKRL 124 Neurospora crassa
AAK93186      18 IHLDMDy-FYAQVEEIRDPTLRSKALGIQQKniVVTCNYVARAKGvTKLMLIAEAQRICPDlvlvnGEDLAPYRQMSQRI 96  fruit fly
XP_757440     62 IALDLDa-FYVSACRKRDPSLVGIPIGIQQKalVATISYEARAAGvGKLDSIKDALKKCPDmilvnGEDLTYFRQVSSQV 140 Ustilago maydis...
EAA62713      14 LHFDYDc-FYASVFEAEQPILKTLPLAVQQKqiVVTCNYEARRRGlRKLQLIKEAKQICPDvviilGEDLTKFRDASKDL 92  Aspergillus nid...
Feature 1                          ##                                                            
2ALZ_A        86 TELLEEFsp--vVERLGFDENFVDLTEMvekrlqqlqsdelsavtv------------------------------sghv 133 human
1ZET_A        84 TELLEEFsp--vVERLGFDENFVDLTEMvekrlqqlqsdelsavtv------------------------------sghv 131 human
NP_001017834 119 TELLMSYcp--lVERLGFDENFMDVTEMvekriketrisdlsfn----------------------------------gh 162 zebrafish
XP_368155    129 YRVLRSFswndrAERLGLDEVFLDVSDMvsynldllnphglansffclsstdpelgfafda-tgfagcvvapswssnssr 207 Magnaporthe gri...
CAD70389     125 YALLRSYswndrVERLGLDEVWMDVSDIieynvellnrhdlehswfcldnkdpekgfafdargfagqvygrekvglegpg 204 Neurospora crassa
AAK93186      97 FDLLLNYtp--lVEKLGFDENFMDVTALvelrqahvaeallrppvgh-----------------------------typa 145 fruit fly
XP_757440    141 WRLVRSIvwekrVEKLGMDELFCDVTEMvdhhlstldkvkeatrwfdlnapkhsqrmddygfeyqawpadppghlhpsda 220 Ustilago maydis...
EAA62713      93 YLFFRRSiwgerVERLGFDEIFMDVTDMitynagllnpndlthsffhldsrdptvgfa-------fdatavygrtypndr 165 Aspergillus nid...
Feature 1                                                                #                       
2ALZ_A       134 ynnqsinlldvlhiRLLVGSQIAAEMREAMYNQlGLTGCAGVAsNKLLAKLVSGVFKPNQQTVLLPEScqhli------- 206 human
1ZET_A       132 ynnqsinlldvlhiRLLVGSQIAAEMREAMYNQlGLTGCAGVAsNKLLAKLVSGVFKPNQQTVLLPEScqhli------- 204 human
NP_001017834 163 vynhesvvvdeeniRLAVGSVIAAEMRQAIFSTlGLTGCAGVAsNKLLAKLVSGSFKPNQQTTLLPHSnaelm------- 235 zebrafish
XP_368155    208 gvvesdeasgllrkRLLLASHLAYRIRLRIEQE-GYTSACGISvNKLLAKMVGSRNKPRNQTTLLALSkadtls------ 280 Magnaporthe gri...
CAD70389     205 tggyeggqagllrtKLLLASHLALHLRLKIEEE-GYTTACGIStNKLLAKLVGNVNKPRNQTTLLSLGpddgeetv---- 279 Neurospora crassa
AAK93186     146 dgtplsncdcgcaqRLAIGTRIAQEIREELKLRlGITCCAGIAyNKLLAKLVGSSHEPNQQTVLVSTYaeqfm------- 218 fruit fly
XP_757440    221 aeylkqnepdwwqqRMLVAARLAYYMRQRISDEvGLTCSAGIApSKSLAKLVGALNKPNQQTAFCPPVvglvsdrckran 300 Ustilago maydis...
EAA62713     166 sgkplseadtqlhlRLLLASHFAGYLRSQLENQkGYTATAGIStSKLLAKVVGSAHKPNNQTTLLPPYsstgdcesn--- 242 Aspergillus nid...
Feature 1                                                                                        
2ALZ_A       207 -------------hslnHIKEIPGIGYKTAKCLEALGInsvrdlqtfs-------------------------------- 241 human
1ZET_A       205 -------------hslnHIKEIPGIGYKTAKCLEALGInsvrdlqtfs-------------------------------- 239 human
NP_001017834 236 -------------ssltGLIKVPGIGYKTREKLKALGLvnvrdlqlyp-------------------------------- 270 zebrafish
XP_368155    281 ------------fvdqhSLRSLPGIGGSMAAVMEQHTRstapdkplkagqvrlqh-----------------------dv 325 Magnaporthe gri...
CAD70389     280 ----------qrfmgshQLRKIPGIGGKTAAALSDYFIsqskpgsssppnpkeitvddlls-----------cpglspsk 338 Neurospora crassa
AAK93186     219 -------------relgDLKRVTGIGQKTQCLLLEAGMssveqlqqcd-------------------------------- 253 fruit fly
XP_757440    301 sieltlerhvtafmdplDLRKLPGFGRVVVDKIRDHLNhdsnasaeqsnrnrqtvsslvepiglsddeeekvkvkvfvga 380 Ustilago maydis...
EAA62713     243 ---------vlrfldsrEIRAIPGIGSKLSRKLISHITasdqsavenkvtirdvraf--------------------pgm 293 Aspergillus nid...
Feature 1                                                                                        
2ALZ_A       242 ------pkilekelgISVAQRIQKLSFGednspv-ilsgpPQSFSEEDSFKKCssev----------------eakNKIE 298 human
1ZET_A       240 ------pkilekelgISVAQRIQKLSFGednspv-ilsgpPQSFSEEDSFKKCssev----------------eakNKIE 296 human
NP_001017834 271 ------lsdlvkefgEMNAKRVQNLACGiddspv-tpagpPQSLSDEDSFKKMstle----------------evlKKIE 327 zebrafish
XP_368155    326 sphllekllqplgleKGIGQKTYRLLHGidtaevkfacdfPIQIGIEDTYANTglsni--------------aqitQQLI 391 Magnaporthe gri...
CAD70389     339 ldsllsslssssstgTGTGKHILSLLQGhdpspvkparsiPTQISIEDTYFSCplvti--------------eqirRELV 404 Neurospora crassa
AAK93186     254 ------mdvmrkkfgFETATRLRDLAFGrdtslv-rpsgkPKTIGMEDACKPIsv--------------------rTDVE 306 fruit fly
XP_757440    381 arkmlsleamvelfgEVIGPRLWALVNGrdaepvipapdfPLQLSIEDTYPYPslrgptihaeigrlslsllrrleLELT 460 Ustilago maydis...
EAA62713     294 gpsllerilggpgapRDIGVKIWGLLHGvdgtqvgeardtPTQISIENSYGHLdtfeavrk--------emlslaaSLIR 365 Aspergillus nid...
Feature 1                                                                                        
2ALZ_A       299 ELLASLLNRvcq------------------------------dgrKPHTVRLIIRRysse-------------khygRES 335 human
1ZET_A       297 ELLASLLNRvcq------------------------------dgrKPHTVRLIIRRysse-------------khygRES 333 human
NP_001017834 328 ELLINLTERmhk------------------------------dgrQPHTLRLTIRKytvtn------------rwfnRES 365 zebrafish
XP_368155    392 KLSASLIRRirvdltepasn--------------idgpeavvwvaRPRTLRLTTRQrhpttgp-------rppynsnRTS 450 Magnaporthe gri...
CAD70389     405 KITGSLLKRmktdlmdd---------------------dkkrwlaRPKTLRLTTRPrtdpkeg--------rgygygRVS 455 Neurospora crassa
AAK93186     307 ERFRMLLKRlveqv--------------------------aedgrVPIAIKVVLRKfdsqk------------ksshRET 348 fruit fly
XP_757440    461 TRDSTLQEQpkplsqettiknevvtirdyqeqppaaaavkagwlrYPLKVRLSVRQgw-----------------nnRVS 523 Ustilago maydis...
EAA62713     366 RMRMDLTERysdaegs----------------------tslgprlREETKARWLARprtfrlstiprstqgsdhtysRRS 423 Aspergillus nid...
Feature 1                                                                               
2ALZ_A       336 RQCPIpshviqklg----------tgnydvmtpMVDILMKLFRNMvnvk--------mpfhLTLLSVCFCN 388 human
1ZET_A       334 RQCPIpshviqklg----------tgnydvmtpMVDILMKLFRNMvnvk--------mpfhLTLLSVCFCN 386 human
NP_001017834 366 RQCPIpnhtglkit----------ggsseavpqLLSLSMKLFHKMvdph--------qpfhLTLLNVCFSN 418 zebrafish
XP_368155    451 RSQPLpsfvhssptq---------paedlaarlVEETLLPMFHKLnppptgt---gpagynIGLINICVTG 509 Magnaporthe grisea 70-15
CAD70389     456 KSQGLpgyvfnngna--------hgddeivqrlVAETLLPMFRELnplnprk---ggkeysIGLLNVCVTN 515 Neurospora crassa
AAK93186     349 KQANIlpslfktsmcpgetgvskvqladgaqdkLLKIVMRLFERIvdms--------kpfnITLLGLAFSK 411 fruit fly
XP_757440    524 KQTKMpveifeldq-----------pteiraaaIEKACRGLLRALiggdn------vigegMNLINIAALE 577 Ustilago maydis 521
EAA62713     424 HSASLpqyvf------------------nldepIDALAERVVRELatpmfrklhrertgwnLRVLNLAVTN 476 Aspergillus nidulans FGS...

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