2AQ4,3GQC


Conserved Protein Domain Family
PolY_Rev1

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cd01701: PolY_Rev1 
Click on image for an interactive view with Cn3D
DNA polymerase Rev1
Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Statistics
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PSSM-Id: 176455
View PSSM: cd01701
Aligned: 16 rows
Threshold Bit Score: 472.569
Threshold Setting Gi: 2660674
Created: 6-Mar-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active siteDNA binding
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               #                                                                      
2AQ4_A      11 FLTSYFAHSRLHHLSAWKANLKDKFLNenihkyt------------------------------------kitdkdtYII 54   baker's yeast
NP_199288  326 FVENYFKNSRLHFIGTWRNRYRKRFHGssnglkwads------------------------------gqntaemakkSTI 375  thale cress
EAL23513   375 FIDGYYQNSRLHHLSTWKAELKVLVRDaqkrseealakaavkelaeggeehgqavhslaksvlpsiplpavpsdsanRVI 454  Cryptococcus neo...
EAK83342   477 FLAGYFAKSRLHHLSTWKSSLQDMVSSalreagrplg-------------------------------ssdlpkgvqRVI 525  Ustilago maydis 521
CAG78000   475 FISHFYEKSRLHHLSTWKARLQAKFELssknkqvp-----------------------------------ppisggdRII 519  Yarrowia lipolyt...
XP_654241  134 FLDKYYLSSRLHHISLWKTIFSDFLSEkvegi-----------------------------------------ksnySKI 172  Entamoeba histol...
XP_456121  173 FINAFFKSSRLHHLSNWKFQLRDNFVKefilsyn------------------------------------dtipdqkYVY 216  Kluyveromyces la...
NP_984465  155 FIEDFFAKSRLHHLSAWKAALRERFLDrhlrmnega---------------------------------lndlrappAHV 201  Eremothecium gos...
CAJ08961    14 SLQHFQQHSRLYFIGQWRTKMEDVFREwfvahpew----------------------------------nhgtleqqRTF 59   Leishmania major
EAN77867    17 SLKHFQQHSRLHFIGQWKTKAHDLFKEwfaenpaw----------------------------------nhgvldrqAIF 62   Trypanosoma brucei
Feature 1         ######                                                      ##  #  #     #   
2AQ4_A      55 FHIDFDCFFATVAYLCrsssfsacdFKRDPIVVCHgtk---------------------nsdIASCNYVARSYGIKNGMW 113  baker's yeast
NP_199288  376 IHIDLDCFFVSVVIKNrl------eLHDKPVAVCHsdnpk------------------gtaeISSANYPARAYGVKAGMF 431  thale cress
EAL23513   455 FHVDFDAFFVSCGLATrp------hLKGKPTVVCHssgrga----------------gstseIASCSYEAREKGVRNGMS 512  Cryptococcus neo...
EAK83342   526 MHIDFDSFFVAVGLKKrp------dLKDKPVVVCHgtgfglpaakagdenrpadnqssstseIASCSYKAREFGIRNGMS 599  Ustilago maydis 521
CAG78000   520 MHLDFDSFFVAVSTLDrp------dLTRKPVAVGSggsr--------------------nadIASCNYEARKYGVKNGMW 573  Yarrowia lipolyt...
XP_654241  173 MHVDIDSFFASVTISKht------eLQGKPVVVSHggn---------------------nadVSSCNYEARKYGIKNGMW 225  Entamoeba histol...
XP_456121  217 FHIDFDCFFAQVSAKIdgk----ydIHATPIAISHgin---------------------nsdISSCNYVARSFGVYNGMW 271  Kluyveromyces la...
NP_984465  202 LHVDFDCFFATISGIKagy----psKAEVPLAVCHggs---------------------ssdVASCNYAARECGIQNGMW 256  Eremothecium gos...
CAJ08961    60 VHIDMDAFFCSVQLAKpey----ahLKTKPVGIAAgky---------------------nsdISSCNYVARSYGIHAGMY 114  Leishmania major
EAN77867    63 AHLDMDAFFCSVVLAReen----ahLRDKPVCVAAgrg---------------------nsdISSCNYVARSFGVRAGMY 117  Trypanosoma brucei
Feature 1                                                          ##                          
2AQ4_A     114 VSQAEKMLPngiklislpytFEQFQLKSEAFYSTLkrln---ifnlILPISIDEAVCVRiipdni--------------- 175  baker's yeast
NP_199288  432 VRHAKDLCPqlvi---vpynFEAYEEVADQFYDILhrh-----crkVQALSCDEAFLDVsdls----------------- 486  thale cress
EAL23513   513 LGRARELVGp----------DLKTMPFSLAFYTVLmay-----adeLQAVSVDEALIDVtshvaaraalpqe-------- 569  Cryptococcus neo...
EAK83342   600 LGQAKRLCShvet---ipydFEAYNSVSLTFYTFLleh-----sdaLQAVSVDEALVDVslllqsmrdgdtndgslyqry 671  Ustilago maydis 521
CAG78000   574 MSRALKLCPdlqt---vdynFPKYEEVSEAFYTILresq----ldyIRPVSVDEALVDAttlieana------------- 633  Yarrowia lipolyt...
XP_654241  226 LKQAYNLCPqliv---vgydFDEYEKVALKMYEILtky-----fhnIEVLSCDECIIDIssv------------------ 279  Entamoeba histol...
XP_456121  272 VSSALKLCPdlki---ldyeFDEYEAISNLFYSALkdig---ifkmVLPISIDEAIGCMlitpd---------------- 329  Kluyveromyces la...
NP_984465  257 VSKAKNLCPqlvc---lpytYDEYETISNAFYEVLeglk---afdaVYPISVDEAVCVIdmpl----------------- 313  Eremothecium gos...
CAJ08961   115 VNSAKERCPelvt---lgydLEACERVAKTLYQIIfdlfptgvkmsMEVYSIDEVMLAAdttd----------------- 174  Leishmania major
EAN77867   118 VNAARVLCPdlrv---lsydLERSGEVAMCLYRMIfelcpahltmsVEVYSVDEVMIAFdtds----------------- 177  Trypanosoma brucei
Feature 1                                                          #                           
2AQ4_A     176 -------hntntlNARLCEEIRQEIFQGtngcTVSIGCSdSLVLARLALKMAKPNGYNITFKSNlseef---------ws 239  baker's yeast
NP_199288  487 ----------dveTEVLASTIRNEILETtg-cSASAGIGgTMLMARLATRVAKPAGQLYISAEKveef----------ld 545  thale cress
EAL23513   570 -tavgkkgeerdpAVELAEKIRDDVREVtdgcEVSIGIAhNILLAKLATRHAKPAGVYHLISEAipsf----------vh 638  Cryptococcus neo...
EAK83342   672 rdhlgsqgqvwtaEKQLAEAFRDEIRDRce-cEASIGIGsNILLARLATRKAKPGGSFHLTDDAkqlf----------ln 740  Ustilago maydis 521
CAG78000   634 -----tecslseaAETVAKAIATKVRATtg-cNISVGCSrNILLAKLALREAKPNGVHVMFDQKaisql---------ld 698  Yarrowia lipolyt...
XP_654241  280 ----------pepIDIFLQQVRIEIKETih-cGISFGIGiNPLTARLAMKKAKPDGIYYLLSNIeefi-----------s 337  Entamoeba histol...
XP_456121  330 --------itselLTQKCMQVRNAVYNVtqgySVSVGCGpSVLLARLALRKGKPNGFHISWFDTvtecha-------fid 394  Kluyveromyces la...
NP_984465  314 ---------dtlsCQNLCIRIRSEVAERtggcSVSVGCApSLVLARLALKRAKPDGYLVCTKESikggdtqvvspahlfe 384  Eremothecium gos...
CAJ08961   175 ----------yalLQPFCETVRRELFSAtg-cTASCGIGpNIMLARVATDRGKPNGICVVHAKDvaqt----------mt 233  Leishmania major
EAN77867   178 ----------yeaVEDYCNEVRRELEAAtk-cTVSCGIGpNVMLARIATQSAKPDGTFIVRPEDvtai----------vs 236  Trypanosoma brucei
Feature 1                                                                                      
2AQ4_A     240 sfKLDDLPGVGHSTLSRLESTFDsphslndlrkr----------ytldalkasvgSKLGMKIHLALQGQDdeeslkily- 308  baker's yeast
NP_199288  546 qlPVGTLPGVGSVLKEKLVKQNIqtcgqlrlis-------------kdslqkdfgVKTGEMLWSYSRGLDlrsvt----- 607  thale cress
EAL23513   639 rlSVEDFPSVGYSTKSKVEAAFKttkagelldi------------skgrwksvlgEKTGEMLWGYLRGIDsrkle----- 701  Cryptococcus neo...
EAK83342   741 alDVDDLHGIGWSLRDRIRELFQtinigeilskt-----------nkrrmmaefgPKKGKMIWDRMHGIDadrle----- 804  Ustilago maydis 521
CAG78000   699 ktKISEIPGIGRSIAGQIREKLHvstagklarlk----------dvipnmsallgPKRGPQIVDFCLGKDdsqia----- 763  Yarrowia lipolyt...
XP_654241  338 tiKIAELPGFGYSLSEQCKIIGVytcgdislis-------------kevlekqigEKNTLKLIEMSKGIDntqfe----- 399  Entamoeba histol...
XP_456121  395 hfKLKDLPGIGYSTDLKIKEQFGnvqnisdlrdlle------rpsnvsklikvigSKNTKKLQIYLDGKDddesismvk- 467  Kluyveromyces la...
NP_984465  385 tcALRDLPGVGRAIAEKLLLGSTghehtiaqvsql---------fsrseliarlgSITGAKLADLFQGLDdvessrmlc- 454  Eremothecium gos...
CAJ08961   234 slPLKYIHGAGASTMEKICRALReqgildertdaggvtcgsvqklkrealqrilgKRQGETFYHLVRGEDgrvvtrtgdp 313  Leishmania major
EAN77867   237 qlPFSEIHGAGSSTIAKLVPLLGdylgdmegvgeenilcqhvqkltkqqlqrvlgQKTGENFYNLCRGNDvrlvtrtgde 316  Trypanosoma brucei
Feature 1                                                                                      
2AQ4_A     309 ---dpkevlqrKSLSIDINWGIRFknitQVDLFIERGCQYLLEKLNEinkttSQITLKLMRRCkdapieppkymGMGRCD 385  baker's yeast
NP_199288  608 ------avqesKSIGAEVNWGVRFrdqqDVQHFLQCLCKEVSLRLQGcemigRTFTLKIKKRKkdaee-ptkymGCGDCD 680  thale cress
EAL23513   702 ------gdkvrKSVSAEMNYGIRFqtqvQAEQYVHDLAAEVSKRMKNvgvkgRQITLKLLKRHpdapveppkflGHGWCE 775  Cryptococcus neo...
EAK83342   805 ------gnklrQSVGSHVNYGIRFlteqEAENFVTGMCQEVSQRAKAvklrgRQVSVQVMVRAktapveapkflGHGVCD 878  Ustilago maydis 521
CAG78000   764 ------vqderKSISVEISYGVRFenkkQFDGFVFNMSAELASRMEEisvegSQLTCKLYVRKnehet--pkymGHGQCD 835  Yarrowia lipolyt...
XP_654241  400 ------nkkekKSVGISINWGIRFenddDVKQFIFRMCCEVEKRLHKsnvmgTLMTMQIMTRKegsge-aikykGHGIVN 472  Entamoeba histol...
XP_456121  468 ---dphtyfqkKSLSVEINYAIRFktidDVDIFLERLALHLWEKLRDsnlmtRQITLKLMKRAqgapvdppkfmGMGKCD 544  Kluyveromyces la...
NP_984465  455 ---nprayfarKSLSVEINWGIRFdavpEVDAFLDRLVQYLVGKLNElrmttSHIVLKIARRSrdapieppkylGMGDCD 531  Eremothecium gos...
CAJ08961   314 edqrhlgkripNAVGCSMNYAVRPegvdDVWKIVRQLLEDVCTKLRRtdavaQGLRVTLLERHplhpketqkymGRGKCV 393  Leishmania major
EAN77867   317 enqrlmgkrtrASIGCSMNYAVRPktveDVWSIARQLLDVVCAKMGRgpyccSGLRVTILERHplhpketlkymGRGKCL 396  Trypanosoma brucei
Feature 1                                                                                      
2AQ4_A     386 SFSRSSRLgiptn----------------------------------------efgiIATEMKSLYRTLgcppmeLRGLA 425  baker's yeast
NP_199288  681 NLSRSITVpaatd----------------------------------------dievLQRISKKLFGSFcldvkeVRGVG 720  thale cress
EAL23513   776 TYNRSSTLsapgggp------------------------------------tddpkvLGTEGVKLLRSLsidpveLRGVG 819  Cryptococcus neo...
EAK83342   879 THHRSAPVsgpggva------------------------------------iddearIRAAAWPLIRDLkadpkeLRGIA 922  Ustilago maydis 521
CAG78000   836 IYNKSVSLgcsvs----------------------------------------stseINLAARGQFAAMgydpvlVRGVG 875  Yarrowia lipolyt...
XP_654241  473 MQSKSITIcetq-----------------------------------------nsaiLNKSAIILWNQFqipikdLRGIS 511  Entamoeba histol...
XP_456121  545 ALSKVSNLgtntq----------------------------------------kfgtIASECKTLFRILsvppeeVRGIG 584  Kluyveromyces la...
NP_984465  532 SYSKSCRLglatn----------------------------------------ipgvISAEIKAAFRMLccpakeLRGIA 571  Eremothecium gos...
CAJ08961   394 EVHLPIKLphamaadeveameleirkavapllvasraisdaeraaqlglspnemdlvIWTVAIASLRDIvv--edLRGMT 471  Leishmania major
EAN77867   397 EFHIPVNFssvlkscdmeimlacveralgpllvlgrtmrdeerveelglqestksgiIWTVGVDTITDIvi--edIRGMS 474  Trypanosoma brucei
Feature 1            
2AQ4_A     426 LQFNKL 431  baker's yeast
NP_199288  721 LQVSKL 726  thale cress
EAL23513   820 IQVTKL 825  Cryptococcus neoformans var. neoformans B-3501A
EAK83342   923 ISLNKL 928  Ustilago maydis 521
CAG78000   876 IQITKL 881  Yarrowia lipolytica CLIB99
XP_654241  512 IQISKV 517  Entamoeba histolytica HM-1:IMSS
XP_456121  585 IQFHRL 590  Kluyveromyces lactis NRRL Y-1140
NP_984465  572 VQFLKL 577  Eremothecium gossypii
CAJ08961   472 VQATGL 477  Leishmania major
EAN77867   475 IQATAV 480  Trypanosoma brucei

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