2Z9H,2HD3,2RCF,2QW7


Conserved Protein Domain Family
EutN_CcmL

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cd01614: EutN_CcmL 
Click on image for an interactive view with Cn3D
Ethanolamine utilisation protein and carboxysome structural protein domain family.
Beside the Escherichia coli ethanolamine utilization protein EutN and the Synechocystis sp. carboxysome (beta-type) structural protein CcmL, this family also includes alpha-type carboxysome structural proteins CsoS4A and CsoS4B (previously known as OrfA and OrfB), propanediol utilizationprotein PduN, and some hypothetical homologous of various bacterial microcompartments. The carboxysome, a polyhedral organelle, participates in carbon fixation by sequestering enzymes. It is the prototypical bacterial microcompartment. Its enzymatic components, ribulose bisphosphate carboxylase/oxygenase(RuBisCO) and carbonic anhydrase (CA), are surrounded by a polyhedral protein shell. Similarly, the ethanolamine utilization (eut) microcompartment, and the 1,2-propanediol utilization (pdu) microcompartment encapsulate the enzymes necessary for the process of cobalamin-dependent ethanolamine degradation, and coenzyme B12-dependent degradation of 1,2-propanediol, respectively, within its polyhedral protein shells. It is interesting that both carboxysome structural proteins CcmL and CsoS4A assemble as pentamers in the crystal structures, which might constitute the twelve pentameric vertices of a regular icosahedral carboxysome. However, the reported EutN structure is hexameric rather than pentameric. The absence of pentamers in Eut microcompartments might lead to less-regular icosahedral shell shapes. Due to the lack of structure evidence, the functional roles of the CsoS4A adjacent paralog, CsoS4B, and propanediol utilization protein PduN are not yet clear.
Statistics
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PSSM-Id: 133473
View PSSM: cd01614
Aligned: 69 rows
Threshold Bit Score: 82.9566
Threshold Setting Gi: 46359636
Created: 12-Dec-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
central poreHexamer/Penta..
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:central pore
Evidence:
  • Structure:2Z9H; Several acidic residues from each subunit of Escherichia coli Ethanolamine Utilization Protein EutN contribute a negative charged hexagonal pore.
    View structure with Cn3D
  • Structure:2RCF; Several residues from each subunit of Halothiobacillus neapolitanus Carboxysome Shell Protein Orfa contribute a positive charged pentagonal pore.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         #                         #  #                  #                         #    
2Z9H_A         1 MKLAVVTGQIVCTVRhhgLAHDKLLMVEMIDpqgn----------pdgqCAVAIDNIGAGtGEWVLLVSG--SSARQAHK 68  Escherichia coli
YP_821621      1 MLIARVVGELVATQKhpsHEGRKLLLVQPLNldgs----------drgdAVVALDAVDAGvGDKVLLATE--GFSAMTSV 68  Solibacter usit...
ZP_02441796    1 MRVAQVVGNIVSTVKdpsHAGQKLMIVDFLDgagr----------rtgeNAVVFDAAQAGvGDVVLVSTD--GGASAIFL 68  Anaerotruncus c...
ZP_01855335    1 MLTGQVIGRATATAKhpsLAGWRLLLVQTLDikgn----------adgfPELVIDRLGCGkGDIVLLTSDg-AAVRDMVG 69  Planctomyces ma...
YP_995464      1 MIRARVNGKLWSTRHldsLPVGALLEVETESg----------------aKLIAFDPLGCAeGESVLVTQG--SVAAAYFP 62  Verminephrobact...
YP_001818181   1 MNYARIDGTIVASAChrsMGGTRTVICQPIDaegr----------degaPILAVDPLGAGlHEVVILSTDg-SATREFVR 69  Opitutus terrae...
YP_708764      1 MIKATVLGPVWATKRldgFPSGALLEVQRDEta---------------eRYVALDHLGSGpGDHVLVALG--SAVAHHLP 63  Rhodococcus sp....
YP_884688      1 MLRATVTGNVWSTRRiegIPAGAFLEVEVEGtg---------------sRMIAFDVLGSGvGEHVLIAQG--SVASSWFT 63  Mycobacterium s...
NP_865034      2 MKIARVIGTVTLSRMhpsMKGYKLRCVEVVEsadqiqvnqddakpiggdTIVAWDLVGSRnGDWVALAEGpeSAAPFRPE 81  Rhodopirellula ...
NP_865036      1 MQPARVIGHTRATVKhesLQGRRLVLVQPTGvgek----------pdgaPLLVLDELGCRvGDRVMLTSEt-GPIREMTG 69  Rhodopirellula ...
Feature 1         #     #        
2Z9H_A        69 SEt-sPVDLCVIGIVD 83  Escherichia coli
YP_821621     69 GRpnsPIDMAVIGFID 84  Solibacter usitatus Ellin6076
ZP_02441796   69 GKh-vIADMTICGILD 83  Anaerotruncus colihominis DSM 17241
ZP_01855335   70 TNn-tPIRWATMGVID 84  Planctomyces maris DSM 8797
YP_995464     63 NRp-vPIDALIIGSID 77  Verminephrobacter eiseniae EF01-2
YP_001818181  70 DPk-sPLRNLIIGIVD 84  Opitutus terrae PB90-1
YP_708764     64 GSt--PADALIIGVID 77  Rhodococcus sp. RHA1
YP_884688     64 GTp-pPIDALIIGSID 78  Mycobacterium smegmatis str. MC2 155
NP_865034     82 VK---PTDASIVAILD 94  Rhodopirellula baltica SH 1
NP_865036     70 FEt-cPARWSVQGLID 84  Rhodopirellula baltica SH 1

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