Conserved Protein Domain Family
PurB

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cd01598: PurB 
PurB_like adenylosuccinases (adenylosuccinate lyase, ASL)
This subgroup contains EcASL, the product of the purB gene in Escherichia coli, and related proteins. It is a member of the Lyase class I family of the Lyase_I superfamily. Members of the Lyase class I family function as homotetramers to catalyze similar beta-elimination reactions in which a Calpha-N or Calpha-O bond is cleaved with the subsequent release of fumarate as one of the products. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits. ASL catalyzes two non-sequential steps in the de novo purine biosynthesis pathway: the conversion of 5-aminoimidazole-(N-succinylocarboxamide) ribotide (SAICAR) into 5-aminoimidazole-4-carboxamide ribotide (AICAR) and; the conversion of adenylosuccinate (SAMP) into adenosine monophosphate (AMP).
Statistics
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PSSM-Id: 176470
View PSSM: cd01598
Aligned: 22 rows
Threshold Bit Score: 666.245
Threshold Setting Gi: 15789662
Created: 15-Jul-2004
Updated: 3-Jul-2019
Structure
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Aligned Rows:
 
active sitetetramer
Feature 1:active site [active site]
Evidence:
  • Comment:By similarity to ASL
  • Comment:The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
NP_287345    23 LRGIFSEYGLLKFRVQVEVRWLQKLAAHAaikev-------pafaADAIGYLDAIvanfseedaaRIKTIE-------RT 88  Escherichia coli...
NP_787919    23 LRGFLSESALIRERIRVEIGWVFFLWRKAvfgl--------aeptKEDERFLSSIvhdah-knipRVKKIE-------ER 86  Tropheryma whipp...
AAL60072     21 LSAFFSEHALIKHRIIVEVRWLLFLNEEElffe---------kvtDHSVEVLNQIatnitdsdiaRVKAIE-------EE 84  malaria parasite...
ZP_00308064  30 LAPYLSEFGLIHYRVKIEIEYFIALCEKPvpql--------kdfdKSIYPALRAIylefsledaqRIKDIE-------KV 94  Cytophaga hutchi...
NP_696947    29 LVEYMSEPALNRERMRVEVEWMILLANGFegngnqpivdgvkpftAEEQAFLRAIpedfgaegikQHAAHE-------AI 101 Bifidobacterium ...
NP_279486    61 LAPYVSEAALMRARVEVEAEYVLALADLDavpl---------elvGDERTALRDAyktfdaadadLVKRIEtegalghAA 131 Halobacterium sp...
XP_636767    29 LSEYFSEYALIKYRVQVEIEYFIELSKLSelkpl------navnkEDDHKKLRDIylqfkesdaqKIKQIE-------KT 95  Dictyostelium di...
AAC71848     25 VSEYFSEYALIKYRIIVEIKWLLFLNDKEyffp---------kvsEKSLSNITSImelindndilRVKKIE-------EE 88  Plasmodium falci...
ZP_00766150  26 LASFFSEAALFRYRVRVEVEYLIFLSRARgihfv-------ppltPPQQAALRALyrqfgdddalAIAAWD-------RR 91  Chloroflexus aur...
YP_063223    23 LGDHLSEAGLNRARVQVEVEWLLYLTNHRmfgs--------splsAEQAGQLRALadgfgqaeidRLAELE-------AT 87  Leifsonia xyli s...
Feature 1         #                               #                                             
NP_287345    89 TNHDVKAVEYFLKEKvaeipe-----lhavseFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYr- 162 Escherichia coli...
NP_787919    87 IQHDVKAVEIFLRERftqqgl------gniveLLHFACTSEDINNLAYALNMKAALSEVILPSIDELIGHLEKMSQKYa- 159 Tropheryma whipp...
AAL60072     85 TNHDVKAVEYFVKEKlknskre---dllkikeYVHYLCTSEDINNVAYATCLKACLNDVVIPCLEKIMLKLKDLAVEYs- 160 malaria parasite...
ZP_00308064  95 TNHDVKAVEYFIKEKlkkydl------gnyleFIHFGLTSQDINNTSIPLLLKDVLNNCIYPELSASIKQIEALAKSWe- 167 Cytophaga hutchi...
NP_696947   102 THHDVKAVEYYIDDQidkapaeltnindelktLVHFACTSEDINNLSIARCVKNAMENVWTPEFKRIIDHLAGKAEEFk- 180 Bifidobacterium ...
NP_279486   132 TNHDVKAVEYFLREHapar----------ahsWIHFGLTSEDVNNLAYRLLVDGAITAVLTPELRGLRDDLVSFAHDYa- 200 Halobacterium sp...
XP_636767    96 TNHDIKAVEYFIKEKmhtelqy----sevvteFIHFGLTSQDINNTAIPLSIVESVEKVLIPQLKQSILEPLRQFAQQwk 171 Dictyostelium di...
AAC71848     89 TNHDVKAVEYFIREKleslkne---eitkvipYVHYLCTSEDINNIAYGLCLYNCIHNIIIPNIQNIIDKLKEFSFNYs- 164 Plasmodium falci...
ZP_00766150  92 VNHDVKAVEYWLRERlttlgl------tghleAVHFALTSEDVNNLAYALMVKEAREMVLLPALESLLERIRQLADEEa- 164 Chloroflexus aur...
YP_063223    88 TKHDVKAVEYLVRERlhtlgl------dhiaeLTHFAATSEDINNLSYALTVSAAVNEVWLPNYRAVIAELRAQAAQHr- 160 Leifsonia xyli s...
Feature 1               #                                                                       
NP_287345   163 DIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGk--INGAVGNYNAHIAAYPEVDW-HQFSEEFVTSLG- 238 Escherichia coli...
NP_787919   160 GDAMLARTHGQPATPTTLGKELLVFVYRLKRQRKNLEDVQFLGk--FSGATGTFSAHCVAAPNYPW-QEWAKEFVNSFG- 235 Tropheryma whipp...
AAL60072    161 HVPLLSRTHGQPASSTTFGKEMANFYARIHHHVGVIRRVKVCAk--FNGAVGNFNAHKVASKDTDWvNTIGLFLKKHFN- 237 malaria parasite...
ZP_00308064 168 TIPMLAKTHGQPASPTRLGKEFMVFVERLINQINLLHQIPYSAk--FGGATGNFNAHNVAYPEINWiEFGNDFVNTTLG- 244 Cytophaga hutchi...
NP_696947   181 DMPLLSLTHGQPATPTTLGKELAVYVYRLNRQLKHIENQEYLGk--INGATGTFGAHLAACPDVDWiAVSREFVTNRMG- 257 Bifidobacterium ...
NP_279486   201 DLPMLARTHGQPATPTTFGKEMAVYAARLGRALARITDAVDGLtgkLAGASGTWAAHTAAFPDVDW-RSFAARFVDSLG- 278 Halobacterium sp...
XP_636767   172 SIPMLARTHGQPATPTTVGKELMVFIERLENQINHLEQSVPHTc-kFGGATGNLNAHKVSYPAIDW-VVFSEKFVKVLHp 249 Dictyostelium di...
AAC71848    165 DVSLLSKTHGQPASPTTFGKEMSNYYYRLYKHINKLKNIEIYVk--FNGAVGNFNAHKVCDPNIDWiDNIKYFIETYFN- 241 Plasmodium falci...
ZP_00766150 165 ATPMLARTHGQPATPTTFGKEMNVFFMRLRRAINDMMAIKITGk--LNGASGVFAAHHVALPQIDW-LKFSRAFVRSLD- 240 Chloroflexus aur...
YP_063223   161 DAAMLARTHGQPATPTTMGKELAVFAHRLERIRKQVEQTEYLGk--FSGATGTFAAHLIADPSADW-LAISEEFVRSLG- 236 Leifsonia xyli s...
Feature 1                #                                                     # #    #         
NP_287345   239 -IQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKtiageIGSSTMPHKVNPIDFENSEGNLGLS 317 Escherichia coli...
NP_787919   236 -LIYNPITTQIEPHDWQCELLERVGHICRILHNLATDMWSYISFGYFQQGfisesVGSSTMPHKVNPIRFENAEANLEMA 314 Tropheryma whipp...
AAL60072    238 -LTYSIYCTQIQDHDYICELCDGLARANGTLIDLCVDIWLYISNNLLKLKvkekeVGSSTMPHKVNPIDFENAEGNLHIA 316 malaria parasite...
ZP_00308064 245 -LSRNQYTTQIEHYDNLAALFDTLKRISTILIDFDRDVWQYVSMNYFKQKvkkdeVGSSAMPHKVNPIDFENSEGNLGVA 323 Cytophaga hutchi...
NP_696947   258 -LTWNPLTTQIESHDWQAELYSTVSHANRIMHNLCVDVWMYISRGVFAQVpvkgaTGSSTMPHKVNPIRFENAEANFEIS 336 Bifidobacterium ...
NP_279486   279 -LAYAEPTTQVNPCDDLTAVFDAVAGANGVLEDLAVDAWLYVSQRYLGQDaadgeTGSSTMPHKVNPIDFENAEGNLAKA 357 Halobacterium sp...
XP_636767   250 sLKRMRFTTQIEHYDNVASLLDAFKRINTILIDLCRDIWTYISMEYFNQKlvkgeVGSSTMPHKVNPIDFENAEGNMGVA 329 Dictyostelium di...
AAC71848    242 -LHFSLYCTQIQDHDYICEISDTLARLNYTLIDLSVDMWLYISSNVLKLKviqkeIGSSTMPHKVNPIDFENAEGNLHLA 320 Plasmodium falci...
ZP_00766150 241 -LEPILLTTQIEPHDTLAALCDALKRIGAILTDLSQDCWRYISDGYLVQAarageVGSSTMPHKVNPIDFENAEGNLALA 319 Chloroflexus aur...
YP_063223   237 -LDWNPLTTQIESHDWQAELYGRISHANRVLHNLCTDIWTYISLGYFRQTpepgaTGSSTMPHKVNPIHFENAEANLELS 315 Leifsonia xyli s...
Feature 1                                                                                       
NP_287345   318 NAVLQHLASKLpVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYg 397 Escherichia coli...
NP_787919   315 CGIISCIVASLsTSRLQRDLSDSSVQRNIGVALGHSLVAIDNLKRGLAGIRLNKNRLTEDLNNNWEVIAEAVQTTIRAEi 394 Tropheryma whipp...
AAL60072    317 NAFFKLFSSKLpTSRLQRDLSDSTVLRNIGSSLAYCLIAYKSVLKGLNIIDIDRRNLEEELNQNWSTLAEPIQIVMKRHn 396 malaria parasite...
ZP_00308064 324 IALFEHFSTKLpISRLQRDLTDSTVLRNIGVPLGHFLIALKSLQKGLSKLELNVDAINADLEDNWAVIAEAIQTILRREn 403 Cytophaga hutchi...
NP_696947   337 CSLLDTLSATLvESRWQRDLTDSTTQRNIGSALGYSLLALTNLMGGLESIHPNTHVIERELDENWEVLGEPIQTAMRACe 416 Bifidobacterium ...
NP_279486   358 NSDLGFLGDSLtTSRLQRDLSDSTVKRNIGAAFAHCLLAYRKLGDGLDAVTPNEAVLREDLRANPEVIGEAVQTILRREg 437 Halobacterium sp...
XP_636767   330 NALYEHLSAKLpISRLQRDLTDSTVLRSIGVPFSHSILSFKSIQRGLSKLVLNEANIAKDLDANWAVVSEAIQTILRREg 409 Dictyostelium di...
AAC71848    321 NSLFKLFSSKLpISRLQRDLSDSTVLRNLGSSFAYSLISYKSLLRGLNKIDVDQNVMNEQLNQNWCTLAEPIQIIMKKYn 400 Plasmodium falci...
ZP_00766150 320 GALLELFSRKLpVSRLQRDLSDSTVLRNLGLAFGYCLLAYQRLLRGLQKVAVDRARLRRDLDAHPEVLAEAIQTILRREg 399 Chloroflexus aur...
YP_063223   316 SAILDSLAANLvTSRLQRDLTDSSAQRNIGVGIGHSVLALDNIQRGLGQISLDADALDADLDTNWEVLAEAIQTVIRADv 395 Leifsonia xyli s...
Feature 1                                                                  
NP_287345   398 ------IEKPYEKLKELTRGkrvdaeg---mkqfidgLALPEEEKARLKAMTPANYIGR 447 Escherichia coli O157:H7 EDL933
NP_787919   395 lacksnEANPYERLKDFTRNkkitkht---ldefitnLDLSEHAKKRLLELTPSKYTGL 450 Tropheryma whipplei str. Twist
AAL60072    397 ------YVDAYEELKQFTRGkvidqkim-qefiktkcAFLPQDVVDQLLELTPATYTGY 448 malaria parasite P. vivax
ZP_00308064 404 ------YPNPYEALKDLTRNggkitkdt--lqgfingLTVSEEIKVELRKLSPHNYTGI 454 Cytophaga hutchinsonii
NP_696947   417 lkglpgMDKPYEKVKGLMRGrtinkeq---veqfidqQSFDPETAARLKALTPATYTGV 472 Bifidobacterium longum NCC2705
NP_279486   438 ------HDDAYERVKALTRGervsldd---fkelfasLDIDADTRDELLALTPERYVGV 487 Halobacterium sp. NRC-1
XP_636767   410 ------FPKPYEALKELTRVsgskkitesdiqtfidsLSIPDDIKSELKLITPFNYIGI 462 Dictyostelium discoideum AX4
AAC71848    401 ------IADSYEQLKNFTRGksidkqcm-yqfiqqncSHLPKNAIDELMNLTPHNYLGY 452 Plasmodium falciparum 3D7
ZP_00766150 400 ------FAEPYELLKSFSRGraltaed---iarfidsLLVSDAVRAELQALAPAAYIGL 449 Chloroflexus aurantiacus J-10-fl
YP_063223   396 tagrstIEDPYALLKSLTRGkrvdhaa---ltefvtgLDIGPAAKNRLLELTPAGYIGL 451 Leifsonia xyli subsp. xyli str. CTCB07

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