1Q5N,1RE5


Conserved Protein Domain Family
pCLME

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cd01597: pCLME 
Click on image for an interactive view with Cn3D
prokaryotic 3-carboxy-cis,cis-muconate cycloisomerase (CMLE)_like
This subgroup contains pCLME and related proteins, and belongs to the Lyase class I family. Members of this family for the most part catalyze similar beta-elimination reactions in which a C-N or C-O bond is cleaved with the release of fumarate as one of the products. These proteins are active as tetramers. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits. CMLE catalyzes the cyclization of 3-carboxy-cis,cis-muconate (3CM) to 4-carboxy-muconolactone in the beta-ketoadipate pathway. This pathway is responsible for the catabolism of a variety of aromatic compounds into intermediates of the citric cycle in prokaryotic and eukaryotic micro-organisms.
Statistics
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PSSM-Id: 176469
View PSSM: cd01597
Aligned: 30 rows
Threshold Bit Score: 388.14
Threshold Setting Gi: 69933166
Created: 30-Jun-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetetramer
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:By similarity to fumarase C
  • Comment:The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits.
  • Structure:1RE5_A, Pseudomonas putida 3-carboxy-cis,cis-muconate lactonizing enzyme homotetramer, active sites.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                ##     
1Q5N_A        7 LYASLFYQRdVTEIFSDRALVSYMVEAEVALAQAQAQVGVIPQSAATVIQRAAktaidkiDFDALATATGLagNIAIPFV 86  Acinetobacter ca...
1RE5_A        5 LFDAYFTAPaMREIFSDRGRLQGMLDFEAALARAEASAGLVPHSAVAAIEAACqae--ryDTGALANAIATagNSAIPLV 82  Pseudomonas puti...
AAV62885      9 LLQNNFSTPaIRSIWSDDNKISKQLAVEAALAKIEGDLGIIPKSAADKIVEKAkvd--nfDFLSLQKESAEkrHSLIALI 86  Streptococcus th...
ZP_00415501  10 LFRDQFGTEaMRRIFSDETTVQRWLDVEAALAKVQGELGIIPRAAADEIRRKArve--liDLGALKAEMDRtaHPIVPLL 87  Azotobacter vine...
NP_252207    10 IHGGAYSSPaFAAIFSDTNQVRRWLDVERALAATQAEMGIIPHEAAREIDRAAqve--rfDLTQLGRESLEtgHLLVPTI 87  Pseudomonas aeru...
ZP_00655580  10 LYGHLWGTDeLRQLFNDEGRIQSWLEILAALAEAQAEVGLVPHEAARSIRAHAdvk--rlDLTRVAELTRStgHSTLGLI 87  Nocardioides sp....
NP_282537    11 LLADSWSTQeMRAIFCEQNRIQKWLDVEAALAKAQAKLKIIPKKAADEIAKKAhyk--fmDMDFIFAEFKKtkHPLVPTV 88  Campylobacter je...
NP_252206    42 THGGGYAGPvSRRIFCSHCRLQRWLDVEAALALAQADVGVIPPEAALEIAKAArls--avDLQQIADGVAStgHSLMPLL 119 Pseudomonas aeru...
CAG77094     10 LIGNNFGTPeMRGVWSEHNRLTKQVEVEVALALAEGELGVIPLDAAKTIAENAdas--alNVEEIAKDAARmkHSLMPTI 87  Erwinia carotovo...
ZP_00739408  13 IFKDQFGTKqMRTNFSDENLIQLWLNSEVALAKAEAKLGIIPQNAATNIANAAdqn--klDFNKIREGMKKtgHPFVAFL 90  Bacillus thuring...
Feature 1                             ###                                            ##         
1Q5N_A       87 KQLTAIVKDadedaARYVHWGATSQDILDTACILQCRDALAIVQNQVQQCYETALSQAQTYrHQVMMGRTWLQQALPITL 166 Acinetobacter ca...
1RE5_A       83 KALGKVIATgvpeaERYVHLGATSQDAMDTGLVLQLRDALDLIEADLGKLADTLSQQALKHaDTPLVGRTWLQHATPVTL 162 Pseudomonas puti...
AAV62885     87 HALQEEVGGk---eGEFVHYGVTTQDIVDTGIMLQTKEAYNIILDHTKDLISTLAKLTKEHrSTLMIGRTHGIHAIPITF 163 Streptococcus th...
ZP_00415501  88 RAIKRVCDGd---aGEYVHWGATTQDIVDSGTVLQLRDGLEEIERDLRGLLDNLLKLAATHrDRVMVGRSHGQQALPITF 164 Azotobacter vine...
NP_252207    88 RALARSCEGs---wGEYVHYGVTTQDILDTGLMLQVKEAWGHALGLLHSIRGHLLALALRHqHTPMVARTHGQQALPTTF 164 Pseudomonas aeru...
ZP_00655580  88 RCLQEILPPs---aQEWVYYGATVQDVSDTWTSLMLKQVAIVLDRDLARVEQATRRLAETHaGTVMCGRTHGQAGLPVTF 164 Nocardioides sp....
NP_282537    89 RGLEKACDNn---lGEYVHFRVTTQDIIDTGLVLQFKEAMTLVKSELKAIAKALAKLAKTHkNTAMMGRTLALQALPITF 165 Campylobacter je...
NP_252206   120 GALQRNCSPs---aREYVHFGATTQDIQDTAQSLEMRDVLDETERALDILLDRLSVLASGTrNALMVARTHSIPALPTTF 196 Pseudomonas aeru...
CAG77094     88 AAIQRQCGAa----GEFIHYGVTTQDIVDTATVLQLKQSFDIVVRDTQLLAVELKRLAKKHqHTLMAGRTHGMQALPTTF 163 Erwinia carotovo...
ZP_00739408  91 HEFESLCDEe---sRKYIHWGATTQDIIDTGLTLQIKGAHELIYNQTQEILKICLRLSQQYkSTVMPGRTLGQHALPITF 167 Bacillus thuring...
Feature 1                                        #                                              
1Q5N_A      167 GHKLARWASAFKRDLDRINAIKar-vlVAQLGGAVGSLASLqdqGSIVVEAYAKQLKLGQTACTWHGERDRIVEIASVLG 245 Acinetobacter ca...
1RE5_A      163 GMKLAGVLGALTRHRQRLQELRpr-llVLQFGGASGSLAALgskAMPVAEALAEQLKLTLPEQPWHTQRDRLVEFASVLG 241 Pseudomonas puti...
AAV62885    164 GFKLSIWLDELLRSQRRLKQLLendvfVGSISGAVGSYASFfgrGREVEKAVLKELGLAVPNISWQPSRDRFSEFASVLG 243 Streptococcus th...
ZP_00415501 165 GFKVAVWAEEIRRDLQRLDQMRer-vlVGQFAGAVGTLSALgehGIAVQEGLFRELGLGCPSIAWHVSRDSLAELASVLA 243 Azotobacter vine...
NP_252207   165 GYKVAVWVDEIDRHLARFDEARer-vlVGNLTGAVGTLASFgaqGFELQRRTLARLGLGAPSTSWHSARDRVLEVAGLLV 243 Pseudomonas aeru...
ZP_00655580 165 GFKAAVWASELARHRERLAEGTar-wsVVQLGGALGTMEFWgeqALPLLDAFAARLGLQAPDIAWLTARDRIAEFVGLLG 243 Nocardioides sp....
NP_282537   166 GHKVAIWLSELDRHFERILELEkr-lyVGSIVGAVGTKASLsdeCNEVEKLTLENLGLEVPNISWQSTRDRFIELGFVLG 244 Campylobacter je...
NP_252206   197 GLKVAGWIDELLRHRERLGEARkr-ilVVQLFGGVGTMAAFgdeAMLLLESFARRLSLDVPLAGWHVSRDRVTEFVSTLA 275 Pseudomonas aeru...
CAG77094    164 GFKLAVWLDEFIRHLERLGEIKer-vlVGNINGAICTYASFgekGPEIERLTLDKLGLNTPNIGWQSARDRFSEYASVTV 242 Erwinia carotovo...
ZP_00739408 168 GYKISIWSDELGRHLERLQQGAdr-ylVGQLGGAVGTLASLgaqGLAVRKEYVKNLNLIEPLVSWHTSRDRFAEFSMILN 246 Bacillus thuring...
Feature 1                                              ## #                              #      
1Q5N_A      246 IITGNVGKMARDWSLMMQtEIAEVFEPtakGRGGSSTMPHKRNPVAAASVLAAANRVPALMSSIYQSMvQEHERSLgAWH 325 Acinetobacter ca...
1RE5_A      242 LVAGSLGKFGRDISLLMQtEAGEVFEPsapGKGGSSTMPHKRNPVGAAVLIGAATRVPGLLSTLFAAMpQEHERSLgLWH 321 Pseudomonas puti...
AAV62885    244 IFSGTLGKIGRELFTLMKtEIDEVHEPfkkGEVGSSTMPQKRNPALIEGLASLTQPVFKDVDLMFQSLlIDGERDAiHWR 323 Streptococcus th...
ZP_00415501 244 IATGSVGKIAHEIYALQKtELGELEEPfspGKVGSSTMPHKRNPAACETVVALARVVRSIVPLALEGVlAEHERDKiVLQ 323 Azotobacter vine...
NP_252207   244 QVSVTLGRVANEIYHLQRsEIDEVREGsrpGQVGSSTMPHKRNPSSVDLVSALSRLVRAQMVALTDAAfQLHERDGtAWR 323 Pseudomonas aeru...
ZP_00655580 244 MLTSTLGKIGQEIYQLQRpELGEVREGahqGVVGSITMPHKVNPEISEHLVTLARVVRSHVGLALEAMlHEHERDGrAWK 323 Nocardioides sp....
NP_282537   245 NINATFNKIAHQLLILSHnEIDEVAEPfgkGQVGSSTMPHKRNPAVSENAVTVSNAFKANLAILSDIErHEHERDGqVWK 324 Campylobacter je...
NP_252206   276 MVTASLARIADEIRTLNRwEIGELEVGwtqQQIGSSTMPHKRNPEGCEQVVVLARLAKAQVLLALDAMiLEHERDYrGTR 355 Pseudomonas aeru...
CAG77094    243 LISGTLGKIGNELYNLMRtEINEIEEPfseGKIGSTTMPHKRNPAALEGLASLTAPLFKSAALIHESMkVEHERDAmSWR 322 Erwinia carotovo...
ZP_00739408 247 MIGATTAKVAEEIISLQGtEIREVEEGyhsGKVGSSTMPHKRNPMTCSYIVGLTKIVNRKSTLAIDSMiQKHERDVtFWQ 326 Bacillus thuring...
Feature 1                                                                                       
1Q5N_A      326 AEWLSLPEIFQLTAGALERTLDVLK-GMEVNAENMHQNIecthGLIMAEAVMMALAPHMGRLNAHHVVEAACKTAvaeqk 404 Acinetobacter ca...
1RE5_A      322 AEWETLPDICCLVSGALRQAQVIAE-GMEVDAARMRRNLdltqGLVLAEAVSIVLAQRLGRDRAHHLLEQCCQRAvaeqr 400 Pseudomonas puti...
AAV62885    324 NEWVALPEITSYLDAQIVNATYILS-GLKVNQDKMLDNLnkqgALPYSERIMFEVGLKLGKQTAHEIVYQAAMTAleenk 402 Streptococcus th...
ZP_00415501 324 TEREYLARLFGLSHAAIAKTRAIAA-GLTVRGAHMEENLyrqrGLLLSEAVMMRLSDALGRQEAHEIVYRICMEVfereg 402 Azotobacter vine...
NP_252207   324 IEWAALPELFVYAGALLTRMEAVLAeGLEVREERMRANLdllgGLILSERVMLALAGQFGKQTAHELLHEITQASqrqgv 403 Pseudomonas aeru...
ZP_00655580 324 TEWIFLPEACILTGTAVAMSCQLLE-ELEVDEPRMARNLadrgGYVMSEPVMRELSPQLGKHAAHELVYQTAMRGreegl 402 Nocardioides sp....
NP_282537   325 MEWKLLPEMFLMLSVVLANMKFALS-DLEVKKDKMLKNLdtlkGFVLAERVMFALSDHYGKQHAHEIVYENAMLGieqqk 403 Campylobacter je...
NP_252206   356 LEWCAVADVSHYALMALSLLTDTID-GLTVHDETMARTAnaysDAICTEAFVFEMAKQLGKSSAYEIIFEITQACqrkri 434 Pseudomonas aeru...
CAG77094    323 AEWIALPEINIYLSAQLQNALGILR-GMSVNEKQMLANLdlqnGLLLSEKVMFEIGKRLGKQTAHHLVYECSMQAfeqnq 401 Erwinia carotovo...
ZP_00739408 327 SEWSYISEICITLSGALEQLKNVLT-NLIVNEEQMKKNLfltkGLIVSENVMLELGKIIGRTTAHDLLYELTMRAfeedk 405 Bacillus thuring...
Feature 1                                                   
1Q5N_A      405 hLKDIISQVdevkqyfnpSQLDEIFKPESYLGNIQDQIDAVLQE 448 Acinetobacter calcoaceticus
1RE5_A      401 hLRAVLGDEpqvsaelsgEELDRLLDPAHYLGQARVWVARAVSE 444 Pseudomonas putida KT2440
AAV62885    403 dFIDLVYSDervrlnftkEDILSWVDPKNNIGECLEKVDEVLSQ 446 Streptococcus thermophilus CNRZ1066
ZP_00415501 403 tLKEALLADplvgdrlspEEIDALLDPKAYTGLAGAFVDRVTAR 446 Azotobacter vinelandii AvOP
NP_252207   404 pFREALLGQprlrgcfsaEALEELLDPVGYVGLAAEMVDLVADK 447 Pseudomonas aeruginosa PAO1
ZP_00655580 403 cFEEVLLVEpairsvlseEAIRAALDPAGAIGAAPEFVRRVLEA 446 Nocardioides sp. JS614
NP_282537   404 tFKEVLLADtrvsqvlkeKDIDALLDATSYVGYAPKLVDEFLAK 447 Campylobacter jejuni subsp. jejuni NCTC 11168
NP_252206   435 pMRQALESDprvgavmpkETLARLFEPRTHLGMAGTIVDKVLDK 478 Pseudomonas aeruginosa PAO1
CAG77094    402 pFKSVLLAHpvlseqvtaADLDEWLNPANYVGSAPQKVDEVIRY 445 Erwinia carotovora subsp. atroseptica SCRI1043
ZP_00739408 406 dLKDILLEDneikkyfsdEEINSLTNPESYTGLSEKFVDDVYNK 449 Bacillus thuringiensis serovar israelensis ATCC 35646

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