1JSW,1FUO,1J3U,1YFE,1FUQ,1YFM,1VDK


Conserved Protein Domain Family
Aspartase_like

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cd01596: Aspartase_like 
Click on image for an interactive view with Cn3D
aspartase (L-aspartate ammonia-lyase) and fumarase class II enzymes
This group contains aspartase (L-aspartate ammonia-lyase), fumarase class II enzymes, and related proteins. It is a member of the Lyase class I family. Members of this family for the most part catalyze similar beta-elimination reactions in which a C-N or C-O bond is cleaved with the release of fumarate as one of the products. These proteins are active as tetramers. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits. Aspartase catalyzes the reversible deamination of aspartic acid. Fumarase catalyzes the reversible hydration/dehydration of fumarate to L-malate during the Krebs cycle.
Statistics
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PSSM-Id: 176468
View PSSM: cd01596
Aligned: 112 rows
Threshold Bit Score: 616.358
Threshold Setting Gi: 78358591
Created: 15-Jul-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitestetramer
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active sites [active site]
Evidence:
  • Comment:The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits.
  • Structure:1JSW_A, Escherichia coli, L-aspartate ammonia lyase homotetramer, active sites
    View structure with Cn3D
  • Citation:PMID 8263924
  • Structure:1FUO_A: Escherichia coli, fumarase C bound to citrate and a water molecule, contacts at 3.5 A
    View structure with Cn3D
  • Citation:PMID 8909293

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1JSW_A        6 RIEEDLLGTREVPADAYYGVHTLRAIENFYIsnnkisdipeFVRGMVMVKKAAAMANKELQTIpksVANAIIAACDEVLn 85  Escherichia coli
ZP_00121889   9 RLEHDCIGQMEVPANVYWGIHTQRAIGNFPVsgitdsqhpeLIRAYATVKRACAIANEELGLIdpaKAEAIRAACLEIEa 88  Bifidobacterium ...
AAS96243     10 RIEHDLLGDCAVPRTALHGAHTQRALSNVALsgrp--lhpeIIRAFGAVKRACARTNMNLGHLsreVAEAIIMACDELEd 87  Desulfovibrio vu...
YP_357044     5 RTEKDLLGQLEVPEQALYGIHTVRALENFPLtgrk--vsraLVHAFGAVKLACTRTNRELGCWpepKAQALEDACTEMMe 82  Pelobacter carbi...
NP_228254     2 RKERDYLGKLEIEGEVYYGIHTKRALMNFPStgek--ldetFIWAYFMVKKACALLNTELGYLderTGNAIVKACDEWEd 79  Thermotoga marit...
YP_390040     2 RTEHDALGSRELPADALYGIHTLRAAENFPVspyr--lappLIRALAQVKLACARTNARLGYLdnrLAEAIETACLEIIg 79  Desulfovibrio de...
NP_393738     2 RTERDVIGEVQLPDNVFYGINTARARENFRItglt--adsdHIVSIAQVKKAAALANADGGALdkeKADYISKACDEIIg 79  Thermoplasma aci...
ZP_00766645  18 RIERDSLGEMQVPANALYGAQTQRAVLNFPVsgmr--pypaFVWAQTMIKRAAAEVNRDLGLLdpqIANAIIQAAEEVLa 95  Chloroflexus aur...
AAT43012      2 RTEHDVIGSVELDDSVYYGINTYRAMNNFKIsgik--edddHIKAIAMVKKAAAYGNHRAGKLndeLYSAIDQACDLVIs 79  Picrophilus torr...
ZP_00400978  16 RIERDTMGEMTVPATALYGAQTARAVENFPVsglr--ahpaFVDATVRIKLAAARTNAGLGLLprrKARAIEQAAREVLa 93  Anaeromyxobacter...
Feature 1                      ####                                       ###                   
1JSW_A       86 ngkcmdQFPVDVYQGGAGTSVNMNTNEVLANIGLELmghqk-geyqyLNPNDHVNKCQSTNDAYPTGFRIAVYSSLikLV 164 Escherichia coli
ZP_00121889  89 gk-ladQFPVDVMQGGAGTSSNMNMNEVIANRALEIagrqr-gdytyIHPNDDVNKSQSTNDTYPAACKLALIDAIgpLA 166 Bifidobacterium ...
AAS96243     88 gg-lgeHIVVDALQGGAGTSANMNVNEVLANRAEEMlggtr-gqyqlVDPLAHVNLHQSTNDVFPTAVRIAALRLLarLE 165 Desulfovibrio vu...
YP_357044    83 gr-lddHIVVDALQGGAGTSTNMNVNEVLANRALQLldkpl-geygtVNPLEDVNRHQSTNDTFPTALKVAAIQQLrlLE 160 Pelobacter carbi...
NP_228254    80 l---kkHVVVDPLSGGAGTSINMNVNEVIANRATEIlggkk--geylVDPIDHVNLHQSTNDTFPTSAKIATIVKLrrLI 154 Thermotoga marit...
YP_390040    80 gs-hqdSFITDPFQGGAGTSANMNMNEVVANRACQLlggvp-gqhslVSPLNHVNMHQSTNDVFPTALRVAMLHELtaLE 157 Desulfovibrio de...
NP_393738    80 gk-yhdQFVIDVYQAGAGTSYNMNANEVIANVALTLmgknk-geysvIHPNDHVNMSQSTNDVYPTMMRLTVSKKIrkLK 157 Thermoplasma aci...
ZP_00766645  96 gq-hadQFVVDPFQAGAGTSHNMNVNEVLANRANQIlgyslddpkkpVSPNDHVNMAQSTNDTIPTAIRLGCLWRLpeLI 174 Chloroflexus aur...
AAT43012     80 gk-yndQFVIDVYQAGAGTSFNMNANEVIANIALEKlghkk-gdykiIHPNDTVNMSQSTNDIYPTMMRVAIMIKApdLL 157 Picrophilus torr...
ZP_00400978  94 gr-hrdQFVVDVYQAGAGTSHNMNVNEVLANRACELlggrr-gdralVDPNDDVNMAQSTNDVVPTAIRLAALELApeVI 171 Anaeromyxobacter...
Feature 1                                ##                                                     
1JSW_A      165 DAINQLREGFERKAVEFqDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNIQRTAellleVNLGATAIGTGLNTPKE 244 Escherichia coli
ZP_00121889 167 ESTKKLAKAFHDLADKHiNDVTIGRTQLQDAVPMTYGQEFHAFATLLKSDLAAFDRVVpllaqLNLGATAIGTGICADLR 246 Bifidobacterium ...
AAS96243    166 PAIAALQSAFQTHEASFaQVVRLGRTQLQDAVPMTFGAACGAWAEALSRDRWRVFKCSerlrvVNLGGTAIGTGIAAPRS 245 Desulfovibrio vu...
YP_357044   161 REVVALLEAFQAQEKALaHVVKIGRTQLQDAALTTLGREMSAYADAFARDRWRIYKCEerlrvVNLGGTAIGTGLAAPRQ 240 Pelobacter carbi...
NP_228254   155 DEVINLQEKIQEKEKEFySIRKPGRTQLMDGPPIMLGQEFGAFADALARDRWRLYKVEerirsVNIGGTAIGTGVGAPKD 234 Thermotoga marit...
YP_390040   158 KETAALQAQLQQLEAAHqDTLRLGRTQLQDAVPMTFGMTFGAWAEAVARDRWRVFKSRerirqVSLGGTAIGTGLGAPRD 237 Desulfovibrio de...
NP_393738   158 DALNELIESLEAKSREFrGIAKPGRTHLQDAAPVTLGLEFGAYAYAMKKDRDEIIDASdyimeLNIGGTAVGTGINTAKN 237 Thermoplasma aci...
ZP_00766645 175 AAIDDLADALEQKAIEFdDVVKSGRTHLQDAVPVRLGQEFGAYAKAVRNDMERIVTAAnrlrrLGIGGTATGTGLNAHPE 254 Chloroflexus aur...
AAT43012    158 ESMNQLIMSLNRKGEEFsKIYKTGRTHLQDAVRVTLGDEFRAWAYALAKDMKEIKNALdyileLNIGGTAVGNGANTPDG 237 Picrophilus torr...
ZP_00400978 172 AALSALAGTFDAKARAWdGVVKSGRTHLMDATPIRLGQEVSGWAAALGAAAGRVRDALpelsvLGLGGTAVGTGLNADAR 251 Anaeromyxobacter...
Feature 1                                                                                       
1JSW_A      245 YSPLAVKKLAevtGFPCVPAEDLIEATSDCGAYVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPelQAGSSIM 324 Escherichia coli
ZP_00121889 247 FRKSAIKHLAqitGLPVTAAPDPVAAMTDMGAYVATSAAIKSLAVHLKKAADDLRLLNSGPRCGFNDLNVParQAGSSIM 326 Bifidobacterium ...
AAS96243    246 YILSVVDRLRedtGLPLARAENLMDATQNVDAFVEVSGILKAHASNLLKLSSDLRLLASGPGGGIGEVVLPpvQAGSSIM 325 Desulfovibrio vu...
YP_357044   241 YIFKVVEKLRsitGLGLARAENLIDATQNVDAFVEVSGILKALATNLLKISGDLRFLSSGPDAGIGELSLPhrQAGSSIM 320 Pelobacter carbi...
NP_228254   235 YILKIVEKVRevtKVKIAKAENLIDATQNWDVFAEVHGLLKSLAVNLYKISNDIRLLGSGPNTVIGELILPavQAGSSIM 314 Thermotoga marit...
YP_390040   238 YILKAADTLRsvtGLKLSRAENLVDATQNTDQLVEVSAILKALAANLLKISTDLRLLGSGPCAGLGELKLPamQAGSTIM 317 Desulfovibrio de...
NP_393738   238 YQENVVKHINeitKENFRKSTNLLGIMQFMTDFSRVMNAVTNLALDVEKIANDIRLLYSGPGAGIHEIIIPavQQGSSIM 317 Thermoplasma aci...
ZP_00766645 255 YHSRMVAKLSeltGHELVSSGNLFESMQSMADPADFSASLRTLCITLGRIANDFRLLSSGPATGLDEIRLPavQPGSSIM 334 Chloroflexus aur...
AAT43012    238 YRDYAVEALSnmlKVNFRTSGNMMGVMQFMTDFSRFMNSLANTAIDLGKISDDLRLLYSGPGAGIREILLPavQQGSSIM 317 Picrophilus torr...
ZP_00400978 252 YRPRVVQELErltGVPLTPAPNPFYAMQSLAPFVALSGALRTAALELLRIGGDVRLLGSGPNTGLGELRLPpvQPGSSIM 331 Anaeromyxobacter...
Feature 1         # #    #                                                                      
1JSW_A      325 PAKVNPVVPEVVNQVCFKVIGNDTTVTMAAEAGQLQLNVMePVIGQAMFESVHILTNACYNLLeKCINGITANkeVCEGY 404 Escherichia coli
ZP_00121889 327 PAKVNPVIPECVNQCCFTIFGMDVTVNWAVAEGQLQLNAFdPVMVHELLTGMALLTHAMETFRvNCVEGIEINadLGRSY 406 Bifidobacterium ...
AAS96243    326 PGKVNPVVCETVAQAAMQATANDLAITLSAQHGQLELNAFmPLIADALLHGIELLTNACDLFRtRCVEGLHPDteTCARH 405 Desulfovibrio vu...
YP_357044   321 PGKVNPVIPEAVSQAAMVVMSNDQTIAQACAMGHLELNPFlPLVADALLGSIDLLNHACSILRrFCVEQLEANeaRCRQH 400 Pelobacter carbi...
NP_228254   315 PGKINPVVSEYVMQICHMVFGHDMILNHACALGNLELNQFaPLIVHLTLKSLTLLTNACRSLA-SYISKIKANneRCEEH 393 Thermotoga marit...
YP_390040   318 PGKVNPVIAEAVSQAALRVLANDGLAAHASAMGTLELNQFmPLLAHTLLESLHLMRSSVTLLNnRCMQGLEPQaeQAQSH 397 Desulfovibrio de...
NP_393738   318 PGKINPSIAEAMNMICHSVIGAQQAVNMSVQAGQLELNVMmPNIDYELTRSIDIMTNGLKMFKqKLIDGIKANvpVCREH 397 Thermoplasma aci...
ZP_00766645 335 PGKVNPVLAEMLNMACYHVQGCDLTVALAAQAGQLELNVMmPIIAHNLFEMMHVLIGAINAFTtKCVVGITANreKAAGW 414 Chloroflexus aur...
AAT43012    318 PGKINPSIPEAMNMICNSVLGAQQAVNMSAKAGQLELNVFmPNIDYEITRSITILKNGMDMLReRAIDGIKPDlkHCRMH 397 Picrophilus torr...
ZP_00400978 332 PGKVNPSMAEMLAMVSYQVVALDGAVAWAASGGQLELNVMmPLVAFDLCHALDILARAVRAFDaRCARGLEADreRARHY 411 Anaeromyxobacter...
Feature 1                                                             
1JSW_A      405 VYNSIGIVTYLNPfIGHHNGDIVGKIcaetgKSVREVVLERglltEAELDDIFS 458 Escherichia coli
ZP_00121889 407 AQSSPSISAALNHyIGYEHAADIAAEavhtgRTVREVAGERtdlpAEQLDEILD 460 Bifidobacterium longum DJO10A
AAS96243    406 VSTSTATLTALVPeIGYAAASEMARHmrehgTDVFGAAKATglidEDRLARLLS 459 Desulfovibrio vulgaris subsp. vulgaris str...
YP_357044   401 VINSTASVTALVSlIGYQKATELAQQvattgRGLRELAIEGglvtAETFDELIS 454 Pelobacter carbinolicus DSM 2380
NP_228254   394 LRRSVSNLTPLIKlFGYEAVSEAIKKanwdiKKAVEILSQEknipVEEILKVLN 447 Thermotoga maritima MSB8
YP_390040   398 VGRSAALAAVLVPlAGHTAVEQMLHTarttgTPFARVAAQAlcvpEDNIRSLLA 451 Desulfovibrio desulfuricans G20
NP_393738   398 LANSFGSAALLNPyLGYDNVAKIVREavetgKSIKSLVLATgkltEEQYMKIMD 451 Thermoplasma acidophilum DSM 1728
ZP_00766645 415 LAKNAILVTALNPvIGYLNGAAVAKEamatgKTIKEVVVEKglltAEQVDELLD 468 Chloroflexus aurantiacus J-10-fl
AAT43012    398 LQNSFGSAALLNPyLGYDTVAAIVERaletgENIRDLVLQTgkitEEQYDKIFS 451 Picrophilus torridus DSM 9790
ZP_00400978 412 AERTVSLATALAPrLGYAAAAEIVKAsvatgRSIVDLAVERggltPAQARRALD 465 Anaeromyxobacter dehalogenans 2CP-C

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