1C3U,1Q5N,1YIS,1DOF,1RE5


Conserved Protein Domain Family
Adenylsuccinate_lyase_like

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cd01595: Adenylsuccinate_lyase_like 
Click on image for an interactive view with Cn3D
Adenylsuccinate lyase (ASL)_like
This group contains ASL, prokaryotic-type 3-carboxy-cis,cis-muconate cycloisomerase (pCMLE), and related proteins. These proteins are members of the Lyase class I family. Members of this family for the most part catalyze similar beta-elimination reactions in which a C-N or C-O bond is cleaved with the release of fumarate as one of the products. These proteins are active as tetramers. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits. ASL catalyzes two steps in the de novo purine biosynthesis: the conversion of 5-aminoimidazole-(N-succinylocarboxamide) ribotide (SAICAR) into 5-aminoimidazole-4-carboxamide ribotide (AICAR) and; the conversion of adenylsuccinate (SAMP) into adenosine monophosphate (AMP). pCMLE catalyzes the cyclization of 3-carboxy-cis,cis-muconate (3CM) to 4-carboxy-muconolactone, in the beta-ketoadipate pathway. ASL deficiency has been linked to several pathologies including psychomotor retardation with autistic features, epilepsy and muscle wasting.
Statistics
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PSSM-Id: 176467
View PSSM: cd01595
Aligned: 114 rows
Threshold Bit Score: 252.425
Threshold Setting Gi: 66963980
Created: 15-Jul-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetetramer
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Each enzyme has four active sites per homotetramer. Each active site is formed by three subunits
  • Structure:1DOF_A; Pyrobaculum aerophilum ASL homotetramer, active sites
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1C3U_A       10 MKDLWTEEAKYRRWLEVELAVTRAYEELGmip-------------kgVTERIRNnaki-----dveLFKKIEek------ 65  Thermotoga maritima
1DOF_A       15 IRRLFTNEAIINAYLEVERALVCALEELGvae-------------rgCCEKVNKasv------sadEVYRLEre------ 69  Pyrobaculum aero...
NP_787919    23 LRGFLSESALIRERIRVEIGWVFFLWRKAvfgla--------eptkeDERFLSSivhda--hknipRVKKIEer------ 86  Tropheryma whipp...
ZP_00308064  30 LAPYLSEFGLIHYRVKIEIEYFIALCEKPvpqlk--------dfdksIYPALRAiylefs-ledaqRIKDIEkv------ 94  Cytophaga hutchi...
NP_696947    29 LVEYMSEPALNRERMRVEVEWMILLANGFegngnqpivdgvkpftaeEQAFLRAipedfg-aegikQHAAHEai------ 101 Bifidobacterium ...
NP_279486    61 LAPYVSEAALMRARVEVEAEYVLALADLDavple---------lvgdERTALRDayktfd-aadadLVKRIEtegalgha 130 Halobacterium sp...
EAN76778     37 LRAFFSEYGFFRYRVLVEVEYFTALCKDVptivpl------rsvtdeQLQKLRKitldcfsvssaeEIKRLErv------ 104 Trypanosoma brucei
XP_636767    29 LSEYFSEYALIKYRVQVEIEYFIELSKLSelkpln------avnkedDHKKLRDiylqfk-esdaqKIKQIEkt------ 95  Dictyostelium di...
ZP_00766150  26 LASFFSEAALFRYRVRVEVEYLIFLSRARgihfvp-------pltppQQAALRAlyrqfg-dddalAIAAWDrr------ 91  Chloroflexus aur...
YP_063223    23 LGDHLSEAGLNRARVQVEVEWLLYLTNHRmfgss--------plsaeQAGQLRAladgfg-qaeidRLAELEat------ 87  Leifsonia xyli s...
Feature 1          #                               #                                            
1C3U_A       66 -tnHDVVAFVEGIGSMige-----------dsrFFHYGLTSSDVLDTANSLALVEAGK-ILLESLKEFCDVLWEVANRYk 132 Thermotoga maritima
1DOF_A       70 -tgHDILSLVLLLEQKsg-------------crYVHYGATSNDIIDTAWALLIRRALA-AVKEKARAVGDQLASMARKYk 134 Pyrobaculum aero...
NP_787919    87 -iqHDVKAVEIFLRERftqqgl------gniveLLHFACTSEDINNLAYALNMKAALSeVILPSIDELIGHLEKMSQKYa 159 Tropheryma whipp...
ZP_00308064  95 -tnHDVKAVEYFIKEKlkkydl------gnyleFIHFGLTSQDINNTSIPLLLKDVLNnCIYPELSASIKQIEALAKSWe 167 Cytophaga hutchi...
NP_696947   102 -thHDVKAVEYYIDDQidkapaeltnindelktLVHFACTSEDINNLSIARCVKNAMEnVWTPEFKRIIDHLAGKAEEFk 180 Bifidobacterium ...
NP_279486   131 atnHDVKAVEYFLREHapar----------ahsWIHFGLTSEDVNNLAYRLLVDGAITaVLTPELRGLRDDLVSFAHDYa 200 Halobacterium sp...
EAN76778    105 -tnHDIKAVEYFIKERmdtcgl------shvteFVHFGLTSQDINNTAIPMMIRDAIVtLYLPALDGIIGSLTSKLVDW- 176 Trypanosoma brucei
XP_636767    96 -tnHDIKAVEYFIKEKmhtelqy----sevvteFIHFGLTSQDINNTAIPLSIVESVEkVLIPQLKQSILEPLRQFAQQw 170 Dictyostelium di...
ZP_00766150  92 -vnHDVKAVEYWLRERlttlgl------tghleAVHFALTSEDVNNLAYALMVKEAREmVLLPALESLLERIRQLADEEa 164 Chloroflexus aur...
YP_063223    88 -tkHDVKAVEYLVRERlhtlgl------dhiaeLTHFAATSEDINNLSYALTVSAAVNeVWLPNYRAVIAELRAQAAQHr 160 Leifsonia xyli s...
Feature 1                #                                                                      
1C3U_A      133 -HTPTIGRTHGVHAEPTSFGLKVLGWYSEMKRNVQRLERAIeev--syGKISGAVGNYANv------pPEVEEKALSYLG 203 Thermotoga maritima
1DOF_A      135 -TLEMVGRTHGQWAEPITLGFKFANYYYELYIACRQLALAEef---irAKIGGAVGTMASwge---lgLEVRRRVAERLG 207 Pyrobaculum aero...
NP_787919   160 -GDAMLARTHGQPATPTTLGKELLVFVYRLKRQRKNLEDVQflg--kfSGATGTFSAHCVaapn-ypwQEWAKEFVNSFG 235 Tropheryma whipp...
ZP_00308064 168 -TIPMLAKTHGQPASPTRLGKEFMVFVERLINQINLLHQIPysa--kfGGATGNFNAHNVaypeinwiEFGNDFVNTTLG 244 Cytophaga hutchi...
NP_696947   181 -DMPLLSLTHGQPATPTTLGKELAVYVYRLNRQLKHIENQEylg--kiNGATGTFGAHLAacpdvdwiAVSREFVTNRMG 257 Bifidobacterium ...
NP_279486   201 -DLPMLARTHGQPATPTTFGKEMAVYAARLGRALARITDAVdgltgklAGASGTWAAHTAafpd-vdwRSFAARFVDSLG 278 Halobacterium sp...
EAN76778    177 -DVPMLARTHGQPASPTNLAKEFVVWIERLREQRRQLCEVPttg--kfGGATGNFNAHLVaypsvnwrAFADMFLAKYLG 253 Trypanosoma brucei
XP_636767   171 kSIPMLARTHGQPATPTTVGKELMVFIERLENQINHLEQSVpht-ckfGGATGNLNAHKVsypa-idwVVFSEKFVKVLH 248 Dictyostelium di...
ZP_00766150 165 -ATPMLARTHGQPATPTTFGKEMNVFFMRLRRAINDMMAIKitg--klNGASGVFAAHHValpq-idwLKFSRAFVRSLD 240 Chloroflexus aur...
YP_063223   161 -DAAMLARTHGQPATPTTMGKELAVFAHRLERIRKQVEQTEylg--kfSGATGTFAAHLIadps-adwLAISEEFVRSLG 236 Leifsonia xyli s...
Feature 1                 #                                                       # #    #      
1C3U_A      204 l--KPEPVSTQvvpRDRHAFYLSTLAIVAAGIERIAVEIRHLQRteVLEVEEPfrkgqrGSSAMPHKKNPITCERLTGLS 281 Thermotoga maritima
1DOF_A      208 l--PHHVITTQvapRESFAVLASALALMAAVFERLAVEIRELSRpeIGEVVEGg----gGSSAMPHKANPTASERIVSLA 281 Pyrobaculum aero...
NP_787919   236 l--IYNPITTQiepHDWQCELLERVGHICRILHNLATDMWSYIS--FGYFQQGfisesvGSSTMPHKVNPIRFENAEANL 311 Tropheryma whipp...
ZP_00308064 245 l--SRNQYTTQiehYDNLAALFDTLKRISTILIDFDRDVWQYVS--MNYFKQKvkkdevGSSAMPHKVNPIDFENSEGNL 320 Cytophaga hutchi...
NP_696947   258 l--TWNPLTTQiesHDWQAELYSTVSHANRIMHNLCVDVWMYIS--RGVFAQVpvkgatGSSTMPHKVNPIRFENAEANF 333 Bifidobacterium ...
NP_279486   279 l--AYAEPTTQvnpCDDLTAVFDAVAGANGVLEDLAVDAWLYVS--QRYLGQDaadgetGSSTMPHKVNPIDFENAEGNL 354 Halobacterium sp...
EAN76778    254 l--KRQQATTQienYDHLAALCDACARLHVILIDMCRDVWQYIS--MGFFKQKvkegevGSSTMPHKVNPIDFENAEGNL 329 Trypanosoma brucei
XP_636767   249 pslKRMRFTTQiehYDNVASLLDAFKRINTILIDLCRDIWTYIS--MEYFNQKlvkgevGSSTMPHKVNPIDFENAEGNM 326 Dictyostelium di...
ZP_00766150 241 l--EPILLTTQiepHDTLAALCDALKRIGAILTDLSQDCWRYIS--DGYLVQAaragevGSSTMPHKVNPIDFENAEGNL 316 Chloroflexus aur...
YP_063223   237 l--DWNPLTTQiesHDWQAELYGRISHANRVLHNLCTDIWTYIS--LGYFRQTpepgatGSSTMPHKVNPIHFENAEANL 312 Leifsonia xyli s...
Feature 1                                                                                       
1C3U_A      282 RMMRAYVDPSLENi-aLWHERDIsHSSVERyVFPDATQTLYYMIVTATNVVRnMKVNEeRMKKNIdltkglvfSQRVLLK 360 Thermotoga maritima
1DOF_A      282 RYVRALTHVAFENv-aLWHERDLtNSANERvWIPEALLALDEILTSALRVLKnVYIDEeRITENLqkalpyilTEFHMNR 360 Pyrobaculum aero...
NP_787919   312 EMACGIISCIVASlstSRLQRDLsDSSVQRnIGVALGHSLVAIDNLKRGLAG-IRLNKnRLTEDLnnnw-eviAEAVQTT 389 Tropheryma whipp...
ZP_00308064 321 GVAIALFEHFSTKlpiSRLQRDLtDSTVLRnIGVPLGHFLIALKSLQKGLSK-LELNVdAINADLednw-aviAEAIQTI 398 Cytophaga hutchi...
NP_696947   334 EISCSLLDTLSATlveSRWQRDLtDSTTQRnIGSALGYSLLALTNLMGGLES-IHPNThVIERELdenw-evlGEPIQTA 411 Bifidobacterium ...
NP_279486   355 AKANSDLGFLGDSlttSRLQRDLsDSTVKRnIGAAFAHCLLAYRKLGDGLDA-VTPNEaVLREDLranp-eviGEAVQTI 432 Halobacterium sp...
EAN76778    330 ALSNALLNFFASKlpiSRLQRDLtDSTVLRnLGVPIGHACVAFASISQGLEK-LMISReTISRELssnw-avvAEGIQTV 407 Trypanosoma brucei
XP_636767   327 GVANALYEHLSAKlpiSRLQRDLtDSTVLRsIGVPFSHSILSFKSIQRGLSK-LVLNEaNIAKDLdanw-avvSEAIQTI 404 Dictyostelium di...
ZP_00766150 317 ALAGALLELFSRKlpvSRLQRDLsDSTVLRnLGLAFGYCLLAYQRLLRGLQK-VAVDRaRLRRDLdahp-evlAEAIQTI 394 Chloroflexus aur...
YP_063223   313 ELSSAILDSLAANlvtSRLQRDLtDSSAQRnIGVGIGHSVLALDNIQRGLGQ-ISLDAdALDADLdtnw-evlAEAIQTV 390 Leifsonia xyli s...
Feature 1                                                                       
1C3U_A      361 LIekg----ltRKEAYDIVQRnalktwnsekhfleylledeevkklvtkeeleelfdiSYYLKH 420 Thermotoga maritima
1DOF_A      361 MIkeg----asRAEAYKKAKEvka-------------------------------ltfEYQKWP 389 Pyrobaculum aerophilum
NP_787919   390 IRaeilacksnEANPYERLKDftrnkkitkhtldefit---nldlsehakkrlleltpSKYTGL 450 Tropheryma whipplei str. Twist
ZP_00308064 399 LRre------nYPNPYEALKDltrnggkitkdtlqgfin--gltvseeikvelrklspHNYTGI 454 Cytophaga hutchinsonii
NP_696947   412 MRacelkglpgMDKPYEKVKGlmrgrtinkeqveqfid---qqsfdpetaarlkaltpATYTGV 472 Bifidobacterium longum NCC2705
NP_279486   433 LRre------gHDDAYERVKAltrgervslddfkelfa---sldidadtrdellaltpERYVGV 487 Halobacterium sp. NRC-1
EAN76778    408 LRre------cYPKPYETLKKltqgntdvteeqvrnfin-gltdisddvraellaitpFTYVGY 464 Trypanosoma brucei
XP_636767   405 LRre------gFPKPYEALKEltrvsgskkitesdiqtfidslsipddikselklitpFNYIGI 462 Dictyostelium discoideum AX4
ZP_00766150 395 LRre------gFAEPYELLKSfsrgraltaediarfid---sllvsdavraelqalapAAYIGL 449 Chloroflexus aurantiacus J-10-fl
YP_063223   391 IRadvtagrstIEDPYALLKSltrgkrvdhaaltefvt---gldigpaaknrlleltpAGYIGL 451 Leifsonia xyli subsp. xyli str. ...

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