1NGV


Conserved Protein Domain Family
E1-1_like
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cd01485: E1-1_like 
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Ubiquitin activating enzyme (E1), repeat 1-like. E1, a highly conserved small protein present universally in eukaryotic cells, is part of cascade to attach ubiquitin (Ub) covalently to substrate proteins. This cascade consists of activating (E1), conjugating (E2), and/or ligating (E3) enzymes and then targets them for degradation by the 26S proteasome. E1 activates ubiquitin by C-terminal adenylation, and subsequently forms a highly reactive thioester bond between its catalytic cysteine and ubiquitin's C-terminus. The E1 also associates with E2 and promotes ubiquitin transfer to the E2's catalytic cysteine. A set of novel molecules with a structural similarity to Ub, called Ub-like proteins (Ubls), have similar conjugation cascades. In contrast to ubiquitin-E1, which is a single-chain protein with a weakly conserved two-fold repeat, many of the Ubls-E1are a heterodimer where each subunit corresponds to one half of a single-chain E1. This CD represents the family homologous to the first repeat of Ub-E1.
Links
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Statistics
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PSSM-Id: 238762
Aligned: 5 rows
Threshold Bit Score: 209.97
Created: 10-Jan-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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dimer
Conserved site includes 28 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interaction [polypeptide binding site]
Evidence:
  • Structure:1NGV; human UBA3-APPBP1 complex
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1      # ####### #                     #  ##  #               ## ### #                 
1NGV_A      12 KYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLqr------------- 78   human
AAS38878    33 LYSRQRYVLGDFAMSKLSKGDVFLSGLGGVGVEIAKNIILAGIKSITLHDTKEASIYDLSSQFYInpe------------ 100  Dictyostelium di...
EAK96752    12 LYDRQIRLWGTSTQLKLRSTKILVINLGAIGSEIVKNLVLGGINTIEILDNSTIQPQDFAAQFFLpnndakvnengdggs 91   Candida albicans...
NP_505604   10 IYDRQIRLWGMEAQNKIRNSKVLIIGGKQLGAEVAKTLSLAGVDEMHLVDHRLVDTEEIGMNFLYdas------------ 77   nematode
EAA38434     4 DYSRTEYVFGSDALHRMQQAEFLIIGADGLAQEIAKNLALTGVSAIRIFDPTSTSYRDLSSSPFLrle------------ 71   Giardia lamblia ...

Feature 1            #          #  ##                                                          
1NGV_A      79 ----ssIGKNRAEAAMEFLQELNsdVSGSFVeespen--lldndPSFFCRftVVVATqlp-esTSLRLADVLwnsqiPLL 151  human
AAS38878   101 ---hvdQKLNRAIISQSHLQELNpyVKVNTItnlslsdlilnnsNSLLQFk-CIILTesn-lnDQIKINEFCkendiKFI 175  Dictyostelium di...
EAK96752    92 gdessyIGQLKLPLVIEKIRELNnrVNLSINtdmti----dqlnGDYLKKfdLIIATeinnkqEIFQLNKLTrdlniPMY 167  Candida albicans...
NP_505604   78 ---vdnSKMTKWAASYNFLYNLNrnVKLFIVeedvls-kndseiEEYLTKftLVVVLdes-yeRTAKVNNICrkhhiRFI 152  nematode
EAA38434    72 ---daeSGAPRDKTIASHIQQLNplCTVEVVdnps-----fssvKELIQPskVVIQTcclpnlPLGHVGDVChaegiPYI 143  Giardia lamblia ...

Feature 1            #                                                                         
1NGV_A     152 ICRTYGLVGYMRIIIkehpvieshpdnaledlrldkpfpelrehfqsydldhmekkdhshtpwiviiakylaqwysetng 231  human
AAS38878   176 VPDCYGLGGWVFNDFgdefkvydkngeklkevfisnisistqnpnqliincmenhihgfeendyiqfkeiigmdqlndqs 255  Dictyostelium di...
EAK96752   168 LTGMHGLFGYIITDLiehesivtkpkgnvsrqsgvqlspnktiinvtthsssssttttttttttndnegsssgkneliti 247  Candida albicans...
NP_505604  153 SGAIYGWIGYAFFDFdghaylvkakspdclneeesetgktstvvtvdeefvletfsypsfvetlnsdftakkivrkckri 232  nematode
EAA38434   144 MCTTNGLSGRLFCDFlarhtildidgerceditlssciieatsshvcnngmrdwrfffstskihdlsdgytvlirdlkfv 223  Giardia lamblia ...

Feature 1                                                                                      
1NGV_A     232 ripktykekedfrdlirqgilknengapedeenfeeaiknvntalnttqipssiedifnddrcinitkqtpsfwilaral 311  human
AAS38878   256 kkfkiniinsncfsinlndnsnnnnsnsnidikslppyqrgg-------------------------------------- 297  Dictyostelium di...
EAK96752   248 kdqfvpiesifvskklp--------------------------------------------------------------- 264  Candida albicans...
NP_505604      --------------------------------------------------------------------------------      nematode
EAA38434   224 avdgsgaqqptvsmerleklvnqqiwtisitghsrfeavpcnssigaaicsflselkqkgfeal---------------- 287  Giardia lamblia ...

Feature 1                                                                                      
1NGV_A     312 kefvakegqgnlpvrgtipdmiadsgkyiklqnvyrekakkdaaavgnhvakllqsigqapesisekelkllcsnsaflr 391  human
AAS38878   298 -------------------------------------------------------------------------iaiqtks 304  Dictyostelium di...
EAK96752       --------------------------------------------------------------------------------      Candida albicans...
NP_505604      --------------------------------------------------------------------------------      nematode
EAA38434   288 ----------------------------------------------------hlrggyvkrvkepldmpflpyseaartp 315  Giardia lamblia ...

Feature 1                                                                                      
1NGV_A     392 vvrcrslaeeygldtinkdeiissmdnpdneivlylmlravdrfhkqqgrypgvsnyqveedigklkscltgflqeygls 471  human
AAS38878   305 iellkfkslnksmiepeiidfdfmkdsrynhlirhsieifkernenqlprefnkddsmefvkivnefnmndyfkfdhlnd 384  Dictyostelium di...
EAK96752   265 -------------------tqltkkqltkklspvlpliftlfeierptidkntepdlpsidiellkqksleichkfeipq 325  Candida albicans...
NP_505604  233 -----------------------------------vptsyflvksmlrassenkltgvtendieklipiwneevaagnht 277  nematode
EAA38434   316 qysdlmvdfsklgrsdvlhsiysviteaasvghtsasallapetvwnselartnldgliafcieykgsrsllemclphlt 395  Giardia lamblia ...

Feature 1                             #   #   #                     #       
1NGV_A     472 vmvkddyvhefcrygaaephTIAAFLGGAAAQEVIKIitkqfvIFNNTYIYSGMSQTSATF 532  human
AAS38878   385 dqikedqlirisnslrgkicPLTSVIGGFVAQEALKSltgkftPLKQWLYIDCFELLEQQQ 445  Dictyostelium discoideum
EAK96752   326 eiiteqyyemfsrcaftefaPISAILGGTVAQDVIQYlsgkesPINNVLILDSITSEMPIY 386  Candida albicans SC5314
NP_505604  278 idmqpvqpdrfdhlfgpnfgPTAACVGGVIGQEAIKSisegknPLRNLFIYTGFESTGFMC 338  nematode
EAA38434   396 nndnrslletflmtyngqisPLVSFMGGWGAQEALKCvsgkytPIHQFYYYECFEALPPKD 456  Giardia lamblia ATCC 50803

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