1NGV,1R4M


Conserved Protein Domain Family
E1-2_like
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cd01484: E1-2_like 
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Ubiquitin activating enzyme (E1), repeat 2-like. E1, a highly conserved small protein present universally in eukaryotic cells, is part of cascade to attach ubiquitin (Ub) covalently to substrate proteins. This cascade consists of activating (E1), conjugating (E2), and/or ligating (E3) enzymes and then targets them for degradation by the 26S proteasome. E1 activates ubiquitin by C-terminal adenylation, and subsequently forms a highly reactive thioester bond between its catalytic cysteine and ubiquitin's C-terminus. E1 also associates with E2 and promotes ubiquitin transfer to the E2's catalytic cysteine. A set of novel molecules with a structural similarity to Ub, called Ub-like proteins (Ubls), have similar conjugation cascades. In contrast to ubiquitin-E1, which is a single-chain protein with a weakly conserved two-fold repeat, many of the Ubls-E1are a heterodimer where each subunit corresponds to one half of a single-chain E1. This CD represents the family homologous to the second repeat of Ub-E1.
Links
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Statistics
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PSSM-Id: 238761
Aligned: 4 rows
Threshold Bit Score: 330.31
Created: 10-Jan-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:forms thioester bond with ubiquitin (-like) molecule
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                      
1NGV_B      50 KVLVIGAGGLGCELLKNLALSgf-----rQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRvp--nCNVVP 122  human
1R4M_B      39 KVLVIGAGGLGCELLKNLALSgf-----rQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRvp--nCNVVP 111  Homo sapiens
P52488      23 RCLLVGAGGIGSELLKDIILMef-----gEIHIVDLDTIDLSNLNRQFLFRQKDIKQPKSTTAVKAVQHFn---nSKLVP 94   baker's yeast
O94609     429 STFLVGAGAIGCEMLKNWAMMgvatgesgHISVTDMDSIEKSNLNRQFLFRPRDVGKLKSECASTAVSIMnpsltGKITS 508  fission yeast

Feature 1                                                                                      
1NGV_B     123 Hfnki-----qdfnDTFYRQfHIIVCGLDSIIARRWINGMLIsllnyedgvldpssiVPLIDGGTEGFKGNARVILPgMT 197  human
1R4M_B     112 Hfnki-----qdfnDTFYRQfHIIVCGLDSIIARRWINGMLIsllnyedgvldpssiVPLIDGGTEGFKGNARVILPgMT 186  Homo sapiens
P52488      95 Yqgnvmd--istfpLHWFEQfDIIFNALDNLAARRYVNKISQfl------------sLPLIESGTAGFDGYMQPIIPgKT 160  baker's yeast
O94609     509 YqervgpesegifgDEFFEKlSLVTNALDNVEARMYVDRRCVff------------eKPLLESGTLGTKGNTQVVVPhLT 576  fission yeast

Feature 1                        #                                                             
1NGV_B     198 ACIECtlelyPPQVNFPMCTIASMPRLPEHCIEYVRMLQWpkeqpfg--------------------------------- 244  human
1R4M_B     187 ACIECtlelyPPQVNFPMATIASMPRLPEHCIEYVRMLQWpkeqpfg--------------------------------- 233  Homo sapiens
P52488     161 ECFECtk--kETPKTFPVCTIRSTPSQPIHCIVWAKNFLFnqlfasetsgneddnnqdwgtddaeeikrikqetnelyel 238  baker's yeast
O94609     577 ESYGSsq--dPPEKSFPICTLKNFPNRIEHTIAWARDLFEglfkqpidnvnmylsspnfletslktssnprevlenirdy 654  fission yeast

Feature 1                                                                                      
1NGV_B         --------------------------------------------------------------------------------      human
1R4M_B         --------------------------------------------------------------------------------      Homo sapiens
P52488     239 qkiiisrdasripeilnklfiqdinkl----------------------------------------------------- 265  baker's yeast
O94609     655 lvtekplsfeecimwarlqfdkffnnniqqllfnfpkdsvtstgqpfwsgpkraptplsfdihnrehfdfivaaaslyaf 734  fission yeast

Feature 1                                                                                      
1NGV_B     245 -------------------------------------------------------------------------egvpldg 251  human
1R4M_B     234 -------------------------------------------------------------------------egvpldg 240  Homo sapiens
P52488     266 -------------laienlwktrtkpvplsdsqintptktaqsasnsvgtiqeqisnfinitqklmdrypkeqnhiefdk 332  baker's yeast
O94609     735 nyglksetdpaiyervlagynpppfapksgikiqvneneeapetaankdkqelksiadslpppsslvgfrltpaefekdd 814  fission yeast

Feature 1                                                               
1NGV_B     252 DDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVF 308  human
1R4M_B     241 DDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVF 297  Homo sapiens
P52488     333 DDADTLEFVATAANIRSHIFNIPMKSVFDIKQIAGNIIPAIATTNAIVAGASSLISL 389  baker's yeast
O94609     815 DSNHHIDFITAASNLRAMNYDITPADRFKTKFVAGKIVPAMCTSTAVVSGLVCLELV 871  fission yeast

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