1FUO,1YFE,1FUQ,1YFM,1VDK


Conserved Protein Domain Family
Fumarase_classII

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cd01362: Fumarase_classII 
Click on image for an interactive view with Cn3D
Class II fumarases
This subgroup contains Escherichia coli fumarase C, human mitochondrial fumarase, and related proteins. It is a member of the Lyase class I family. Members of this family for the most part catalyze similar beta-elimination reactions in which a C-N or C-O bond is cleaved with the release of fumarate as one of the products. These proteins are active as tetramers. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits. Fumarase catalyzes the reversible hydration/dehydration of fumarate to L-malate during the Krebs cycle.
Statistics
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PSSM-Id: 176465
View PSSM: cd01362
Aligned: 67 rows
Threshold Bit Score: 688.853
Threshold Setting Gi: 68192807
Created: 14-Jul-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetetramer
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits.
  • Comment:active site consists of a citrate binding site (site A) and a malate binding site (site B)
  • Structure:1FUO_A: Escherichia coli, fumarase C bound to citrate and a water molecule, contacts at 3.5 A
    View structure with Cn3D
  • Structure:1FUO_A, Escherichia coli fumarase C, bound with malate at site B, contacts at 3.5A
    View structure with Cn3D
  • Citation:PMID 8909293

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1FUO_A        5 RSEKDSMGAIDVPADKLWGAQTQRSLEHFRIStekMPTSLIHALALTKRAAAKVNEDLGLLseeKASAIRQAADEVLAGq 84  Escherichia coli
ZP_00317009  51 RTEKDSLGEVQVPEYALYGAQTQRALNNFKISglvFPRSFIKALGLIKASCAQANKKLGSLepaMANAICDAAIDVANGt 130 Microbulbifer de...
EAN68743      7 RVESDSMGEVSVPKKALYGAQTQRALDNFQFSglvMPKAFIESLALIKQTAAETNAQLGLLdsqRCEAIVAASQAIIDGk 86  Shewanella denit...
NP_636866     6 RIEHDSMGELQVPADALWGAQTQRAVQNFPISgqpMPRGFIRALGLIKAAAAGVNADLGLLsksVAKVVQEAALQVAQGa 85  Xanthomonas camp...
CAG22275     53 RIETDSMGDVHVPENALYQAQTQRAVNNFPISgiqMPHEFIKALAYIKQAAARSNLELKLLddkVAHAIIDSCQEIINGe 132 Photobacterium p...
ZP_00773465  20 SSSSETISGDSQAPEMLYKEQTSLALDNFKISgtpMPSEFIHALALLKASAAYANAEVAGLdseIAIDIQRAAMEVAQGd 99  Pseudoalteromona...
ZP_00586269   4 RIETDSMGEVLVPAEALYGAQTQRALNNFAIGgqpMPRAFIETLLLIKAAAARANSQLGVLasdCGQAITEAALSLRQDk 83  Shewanella amazo...
YP_391653     6 RIEKDSMGSLQVPESALYGAQTQRAIDNFPIShtpMPAIFIKAMAYVKFACSNSNLELGLIseeKANAIQAAAKELTQGq 85  Thiomicrospira c...
Q51404        5 RIERDSMGELAVPATALYGAQTQRAVNNFPVSgqrMPQAFVRALLLAKAAAARANVSLQQLdapMGEAIADTCLQLLQEd 84  Pseudomonas aeru...
YP_155326     6 RTEKDSMGEVKVLSKALYAAQTQRAVDNFSISgltLPTQLIQAIAMVKASAAGANAKIGQLekeQAGAIIKAAKEIINGq 85  Idiomarina loihi...
Feature 1                     # #                         #  ####      ###                      
1FUO_A       85 h-dDEFPLAIWQTGSGTQSNMNMNEVLANRASELLggvrgmeRKVHPNDDVNKSQSSNDVFPTAMHVAALLALRkqLIPQ 163 Escherichia coli
ZP_00317009 131 f-dDQFPLDIFQTGSGTSTNMNANEVIAKLASDKLg------NSVHPNDHVNMSQSSNDVIPSCIHLSAALQIEttLLPA 203 Microbulbifer de...
EAN68743     87 h-lTEFPVDVFQTGSGTSTNMNANEVIARLAAQLSg------LTISANDHVNMSQSSNDVIPTCIHLSAALAISkqLLPA 159 Shewanella denit...
NP_636866    86 h-dAHFPIDVYQTGSGTSSNMNANEVIATLATRAGk------DAVHPNDHVNLGQSSNDVVPTAIRVSALLAVQeqLQPA 158 Xanthomonas camp...
CAG22275    133 h-fEQFPIDVFQTGSGTSSNMNANEVIATLASQKAe------QNISPNDHVNMGQSSNDVIPTAIQVSAAITCHfqLFPA 205 Photobacterium p...
ZP_00773465 100 h-dNAFMVDVYQTGSGTSTNMNANEVIATLAERKLg------RHVHPNDHVNMGQSSNDVIPSAIHVSATLALSqhLIPG 172 Pseudoalteromona...
ZP_00586269  84 almQHFPVDVFQTGSGTSSNMNANEVLARLTSESLg------RAVSANDEVNLGQSSNDVIPSCIQIASAGVLTktLLPA 157 Shewanella amazo...
YP_391653    86 h-aEHFPIDVFQTGSGTSTNMNINEVISNLAKQLTg------TEVHPNDDVNMSQSSNDVVPTAIHVAAAIALEehLLPS 158 Thiomicrospira c...
Q51404       85 f-mQHFPVDVFQTGSGTSSNMNANEVVATLASRRLg------GKVNPNDHVNCGQSSNDIIPSTIHISAALEISerLLPA 157 Pseudomonas aeru...
YP_155326    86 h-lEHFPIDVFQTGSGTSSNMNVNEVIANLAKQNG-------VEIHPNDHVNQSQSSNDVIPTAIQVASVLAVEkqLEPA 157 Idiomarina loihi...
Feature 1                               #                                                       
1FUO_A      164 LKTLTQTLNEKSRAFaDIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYSLPhVAELALGGTAVGTGLNTHPEYA 243 Escherichia coli
ZP_00317009 204 LNNLVDVISIKTQALsSVVKTGRTHLMDAMPLTFGQELSGWAHQIRNNVQRLESALPrLKMLCLGGTAVGTGVNAAPLFG 283 Microbulbifer de...
EAN68743    160 LEHLTSLLEQKAVTLqGLVKTGRTHLMDAMPVSMSQVILGWAWQVKQSQQRLTHGLQqLLSLAQGGTAVGTGVNAHEDFS 239 Shewanella denit...
NP_636866   159 LKHLRKTIDKRAKGLdKIVKTGRTHLMDAMPLTFGQEFGAWSAQLSSAQERIDDSLKrLRRLPLGGTAIGTGINADPRFG 238 Xanthomonas camp...
CAG22275    206 LEYLTQALDKKTAEIgHIVKTGRTHLMDAMPVTLGQELQGWKIQLERAQLGIQHALDnASALAQGGTAVGTGINAEPEFA 285 Photobacterium p...
ZP_00773465 173 LQSLQSAIDAKSILYrDTVKTGRTHLMDAMPITLGQELSGWSKQIQQSISRLIEVLPrISALALGGTAVGTGINTHRDFA 252 Pseudoalteromona...
ZP_00586269 158 LTHLKSCIRTKAASVkGVVKTGRTHLMDAMPVRLSQSLETWASQLDARELQLQSQLIsLWQLPQGGTAVGTGVNAAPGFP 237 Shewanella amazo...
YP_391653   159 LEKLTQTLKNKENELnGIVKNGRTHLMDATPIRLDQEIGAWRHLILDNIERLKDTQKrLHRLPQGGTAVGTGINADPEFS 238 Thiomicrospira c...
Q51404      158 LRHLEQTIQSKAGEVhAYVKTGRTHLMDAMPVRMSQVLGGWAQQVRQAGVHIESVLPaLQQLAQGGTAVGTGINAHPRFA 237 Pseudomonas aeru...
YP_155326   158 LKGLIDTIRKKAIELrNVVKTGRTHLMDAMPMTMAQELEAWAGQLDITAEQLPQLLQrSRVLPQGGTAIGTGINAPKEFA 237 Idiomarina loihi...
Feature 1                                                                                       
1FUO_A      244 RRVADELAviTCAPFVTAPNKFEALATCDALVQAHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPG 323 Escherichia coli
ZP_00317009 284 QSCVTALSelSGMELTITPNKFEGISSQDTALEISSHLKTLAASLMKISNDLRWMNSGPLAGLAEIHLPQLQPGSSIMPG 363 Microbulbifer de...
EAN68743    240 ARFCTVLSakTGVKFAQSQNLFCAIGSQDIAVSVSGELKGCAVSLTKIANDLRWMNSGPLAGLGEIALPELQPGSSIMPG 319 Shewanella denit...
NP_636866   239 GKVAKALStlSSVKFESAENKFEGLAAQDDAVELSGQLNALAVALIKIANDLRWMNAGPLAGLGEIELPALQPGSSIMPG 318 Xanthomonas camp...
CAG22275    286 AIFANNISisTEIPFTSSDNFFYNLSSQDAIVALSGQLKTLAVAILKISNDLRWMNSGPLAGLGEIELEALQPGSSIMPG 365 Photobacterium p...
ZP_00773465 253 ATACRHLSqlTGLRFMPVDNYFSAISSQDTAVEVSGHLNTLAVSLMKICNDLRWMNSGPLTGLGEIALDKLQAGSSIMPG 332 Pseudoalteromona...
ZP_00586269 238 QAFCHELNllTGLAFQPAPHLFTGIASQDTAVALSGELKALAVTLMKIANDLRWMNSGPLAGLGEIRLPALQPGSSIMPG 317 Shewanella amazo...
YP_391653   239 RLVCANLStiTAIDFDPMDNLFVGLSSQDTSVELSGQLNVLASSLMKIANDLRWMNSGPLAGLAEIQLPALQPGSSIMPG 318 Thiomicrospira c...
Q51404      238 ERFSQELNdlTGLAFRPGDDFFALIGSQDTAVAASGQLKTLAVTLMKLANDLRWMNSGPLAGLGEIELEALQPGSSIMPG 317 Pseudomonas aeru...
YP_155326   238 AEFAEQLTqlSGSDFKHSANPFTGIAGQEINLQLSAQLNTLATTLLKVSNDLRWMNSGPLAGLGEIQLTALQPGSSIMPG 317 Idiomarina loihi...
Feature 1       # #    #                                                                        
1FUO_A      324 KVNPTQCEALTMLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNkHCAVGIEPNRERINQLLN 403 Escherichia coli
ZP_00317009 364 KVNPVIPEAVCMAAAQVIGNDATITVAAQSGNFQLNTMLPVIAFNLLQSIQLLANSCNALAtKAIAGLEVNMDNINQVLA 443 Microbulbifer de...
EAN68743    320 KVNPVIPEAVAMIAAQVIGNDTCITIAGQSSNFELNVMLPVIAYNLLQSIELLSNASVALAdKAILGFTVNEHRLKQALS 399 Shewanella denit...
NP_636866   319 KVNPVIPEATVMVCAQVIGHHAAITVAGQTGNFQLNVALPLIAANLLDSINLLSNVSRLLAdTAIAGLKVRQERVREALD 398 Xanthomonas camp...
CAG22275    366 KVNPVIPEAAAMVAAQVIGNDATITVGGQSGNFQLNVMLPVIAHNLIQSIELMANASTLLAdKAISTFVVRKENLKVALA 445 Photobacterium p...
ZP_00773465 333 KVNPVIPEALAMVCAQVMGYHQSITIAGQSGNFQLNTMLPLIGYNLLTSISILSNGINALThKAIVTMRANSDRMAESIS 412 Pseudoalteromona...
ZP_00586269 318 KVNPVIPEAVAMACAQVIGNDACITVAGQSGNFELNVMLPVIADNLLSSLRLLTNAMMALAdKAIDGFEVCEGRMAKELS 397 Shewanella amazo...
YP_391653   319 KVNPVIPEAVCMVAAQIMGNHTAITIGGQAGNFQLNVMLPMIANNLLQSLEIAANAADVLAdKAIAGFTVNEQKIEETLE 398 Thiomicrospira c...
Q51404      318 KVNPVIPEATAMVAAQVIGNDAAIAVAGQSGNFELNVMLPLVADNLLHSIQLLANVSRLLAdKAIASFKVNQGKLSEALA 397 Pseudomonas aeru...
YP_155326   318 KVNPVIPEAVAMVAAQVNGNHTTITTAAQQGNFQLNVMLPVIGYNLLQSINILSNSCEALAsKAIADFEVNEERLQQALA 397 Idiomarina loihi...
Feature 1                                                               
1FUO_A      404 ESLMLVTALNTHIGYDKAAEIAKKAHKEgLTLKAAALALGYLSEAEFDSWVRPEQM 459 Escherichia coli
ZP_00317009 444 KNPILATSLNNEIGYEKSAEIAKQAYSQkRPILDVALEMSGVDKKTLRRLLDPKNL 499 Microbulbifer degradans 2-40
EAN68743    400 LNPILVTALNPIIGYENAAKIAKKAYEQhRPIIDVAEEETNLDRASLEAILDPYKL 455 Shewanella denitrificans OS217
NP_636866   399 RNPILVTALNPIIGYEKAAAIAKRAYKEqRPVLDVAKEDSGLSEAELRRLLDPAAL 454 Xanthomonas campestris pv. campestris st...
CAG22275    446 KNPILVTALNPVIGYLKAAEIAKKAYKEqRAIIEVAEEETNISREELEKLLNPEKL 501 Photobacterium profundum
ZP_00773465 413 MNPILVTALNNRVGYEKGALIAKAAYEEkRPVIDVAAQMTDIPREELEKLLDPKSM 468 Pseudoalteromonas atlantica T6c
ZP_00586269 398 RNPILVTALNPIVGYAKAADIAKRAYAEgRPVLEVALEMTDIDAEELARLLDPVRL 453 Shewanella amazonensis SB2B
YP_391653   399 TNPILVTSLNRLVGYETGAAIAKKAYETgQPVFDVAKAMTDLDEETLKKYLNPALL 454 Thiomicrospira crunogena XCL-2
Q51404      398 RNPILVTALNPIIGYQKAAEIAKQAYREgRPIIDVALENTDLDRARLEVLLDPEKL 453 Pseudomonas aeruginosa
YP_155326   398 KNPILVTALNPVIGYNKAAEIAKVAYKDgRAIIDVADEMTDLGRDRLEHLLDPKKL 453 Idiomarina loihiensis L2TR

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