1C3U,1DOF


Conserved Protein Domain Family
Adenylsuccinate_lyase_1

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cd01360: Adenylsuccinate_lyase_1 
Click on image for an interactive view with Cn3D
Adenylsuccinate lyase (ASL)_subgroup 1
This subgroup contains bacterial and archeal proteins similar to ASL, a member of the Lyase class I family. Members of this family for the most part catalyze similar beta-elimination reactions in which a C-N or C-O bond is cleaved with the release of fumarate as one of the products. These proteins are active as tetramers. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits. ASL catalyzes two steps in the de novo purine biosynthesis: the conversion of 5-aminoimidazole-(N-succinylocarboxamide) ribotide (SAICAR) into 5-aminoimidazole-4-carboxamide ribotide (AICAR) and, the conversion of adenylsuccinate (SAMP) into adenosine monophosphate (AMP).
Statistics
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PSSM-Id: 176464
View PSSM: cd01360
Aligned: 42 rows
Threshold Bit Score: 440.065
Threshold Setting Gi: 13096685
Created: 14-Jul-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetetramer
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Each enzyme has four active sites per homotetramer. Each active site is formed by three subunits
  • Structure:1DOF_A; Pyrobaculum aerophilum ASL homotetramer, active sites
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                #    
1C3U_A      4 RYSLSPMKDLWTEEAKYRRWLEVELAVTRAYEELGmi-pKGVTERIRNNaki----------dveLFKKIEEKTNHDVVA 72  Thermotoga maritima
1DOF_A      9 RYGSEEIRRLFTNEAIINAYLEVERALVCALEELGva-eRGCCEKVNKAsvs-----------adEVYRLERETGHDILS 76  Pyrobaculum aeroph...
NP_276650   9 RYGTPEMRVIWEAENKLQKMLDVEAALAQAEGELGii-pAEAASEIVRKastef-------vtleRVNEIERDTKHDIAS 80  Methanothermobacte...
NP_142783   9 RYGSEEMRKIWDEKNKLQKLLDVEAALARAHAKVGni-pEESAKVISERantkw-------vkleRVKEIEAEIHHDIMA 80  Pyrococcus horikos...
NP_247924  21 RYGTPEMRKVWEEENKLEKMLKVEAALAKAQAELGli-pKEAAEEINKKastky-------vkleRVKEIEKQTKHDVVA 92  Methanocaldococcus...
NP_614368   9 RYGSDELRRVFSEENKVAKMLEVEAALVRALSEVFdfvpEEAADEVERVveeitgdeeelrrfveRVKEIEAEIKHDVMA 88  Methanopyrus kandl...
NP_618841  12 RYGTAEMKYVWSQENRLAKILQTEAALALAEADMGli-pAEAAEIISECvpsv---------kaeRVDEIEAEIHHDMMA 81  Methanosarcina ace...
NP_071067   9 RYGTPEMKRIWSEDSKIKRMVRVEMALLRALAKKGyl-sEEEAKEAKKKaysv---------tpeRVKEIEAEIKHDIMA 78  Archaeoglobus fulg...
NP_341790  28 RYGSNEMRKFFSREEILKRRIQVEIALLKALTKYGlv-kEEDVQKVEKAslti---------rpeEVDELESKIGHDVMA 97  Sulfolobus solfata...
AAT43401    9 RYGRDNVKYIFSEENRMRLMLRVEAALAQAEYEYNii-sRDAFLDIKNAvdsns-------vrieRVHEIESRIHHDTMA 80  Picrophilus torrid...
Feature 1                      #                                                   #          
1C3U_A     73 FVEGIGSMige-dsrFFHYGLTSSDVLDTANSLALVEAGKILLESLKEFCDVLWEVANRYKHTPTIGRTHGVHAEPTSFG 151 Thermotoga maritima
1DOF_A     77 LVLLLEQKsg---crYVHYGATSNDIIDTAWALLIRRALAAVKEKARAVGDQLASMARKYKTLEMVGRTHGQWAEPITLG 153 Pyrobaculum aeroph...
NP_276650  81 LVKALAEQcegdageYVHFGATSNDIVDTSNSLLLRDSISVLRDKLTRVLEVLLALADENRDRVCIGRTHGQHALPTTYG 160 Methanothermobacte...
NP_142783  81 VVKALSEVcge-hgkYVHLGATSNDIIDTANALLIKESLEIVERDLKELRSILKQLAEKHIGTVCIGRTHGQHAVPTTYG 159 Pyrococcus horikos...
NP_247924  93 MIRALAEVcegnageYIHFGATSNDIVDTANSLLIKESIEIIEDKLKQLRDILLDKAEEHKYTVCVGRTHGQHAIPTTYG 172 Methanocaldococcus...
NP_614368  89 LVKALSERcev-ggdYVHLGATSNDVIDTAHALVLREALSIIYRRLHRLAEVLAEKAEEYADLPMVGRTHGQHAVPTTLG 167 Methanopyrus kandl...
NP_618841  82 VVVAISEKcredagkWVHFGATSNDILDTATALQIKDAIDLLEDKLKILLRVLVNQAEAHKNTVCCGRTHGQIGVPTTYG 161 Methanosarcina ace...
NP_071067  79 LVKAITEAtg---crWVHFGATSNDIIDTATALQLRDSLKILEVKIKRLAKVLADKALEYKDVVCLGRTHGQAALPTTYG 155 Archaeoglobus fulg...
NP_341790  98 MVVLMAERsgd-sgrYVHFGATSYDIVDTAYALVFRDAIRLVKTKVVEILEMMVSLAIKYKDLAMVGRTHGQHALPITLG 176 Sulfolobus solfata...
AAT43401   81 LVEALTEQcsa-gknFVHFGVTSNDIIDTATAMQIKSAVKIIKDDIKNLMKTLIKLIDENKDSVMVGRTHGQHASPITFG 159 Picrophilus torrid...
Feature 1                                                                    #                
1C3U_A    152 LKVLGWYSEMKRNVQRLERAIEEVsYGKISGAVGNYANVP---PEVEEKALSYLGLKPEPVSTQVVPRDRHAFYLSTLAI 228 Thermotoga maritima
1DOF_A    154 FKFANYYYELYIACRQLALAEEFI-RAKIGGAVGTMASWGelgLEVRRRVAERLGLPHHVITTQVAPRESFAVLASALAL 232 Pyrobaculum aeroph...
NP_276650 161 MKFAIWADEIHRQLERLDACSERLcVGMMTGAVGTTAALGeegLEVHERVSEILGLRPVLISNQVVQRDNHAEFIMVLAN 240 Methanothermobacte...
NP_142783 160 MKFALWLDEIQRHIERLYQLKERVlVGKMRGAVGTAASFGekaFEIERLVMEDLGLKPARITNQIVQRDVYAELLFFLAL 239 Pyrococcus horikos...
NP_247924 173 MRFALWAAEIDRHLERLKEAKKRIcVSMITGAVGTMAAMGekgLEVHKRVAEILGLEPVLISNQVIQRDRHAEFVFLLAL 252 Methanocaldococcus...
NP_614368 168 MKFAIWAREVVRHLKRLRECANRVlVGQLSGAVGTMAALGekgPEVQRRVMELLNLRPVTVSNQVIQRDRYAELIALLAL 247 Methanopyrus kandl...
NP_618841 162 LRFAIWASEISRHLDRLYELTPRAtVGQMTGAVGTQAAFGksgILIQKLTMQHLGIGAVDVSNQIIQRDRHAEFVMWMAN 241 Methanosarcina ace...
NP_071067 156 FRFALWAAEVARHYIRLQQMKDRLlVGQMSGAVGTQAAFGkdgFEIEEEVMRLLNLKPALISSQIIPRDSYCEYLEFLAN 235 Archaeoglobus fulg...
NP_341790 177 FKIANYIYEFSRSLERLNEVEKRIvKGKMAGAVGTMAAWGnigLEIEENVMKFLGLRPHEISTQVAPRDAFAELTSDLAI 256 Sulfolobus solfata...
AAT43401  160 LKLSVYLDEMSRHLKRLTEAGDRIvAGKISGPVGTGASLGkdsIEIQNRVCEILGIKSEIASSQIVCRDRYIEYLSIINN 239 Picrophilus torrid...
Feature 1                                             # #    #                                
1C3U_A    229 VAAGIERIAVEIRHLQRteVLEVEEPfrk-gqRGSSAMPHKKNPITCERLTGLSRMMRAYVDPSLENIaLWHERDISHSS 307 Thermotoga maritima
1DOF_A    233 MAAVFERLAVEIRELSRpeIGEVVEGg-----GGSSAMPHKANPTASERIVSLARYVRALTHVAFENVaLWHERDLTNSA 307 Pyrobaculum aeroph...
NP_276650 241 IATTLDKIALEIRNLQRteIMEVGEKfdpekqVGSSTMPHKMNPITAERICGIARVIRSYVVAALENNpLWHERDLTNSS 320 Methanothermobacte...
NP_142783 240 VASTLDKMGLEIRNLQRteILEVSEPfge-kqVGSSTMPHKRNPIRTEKVCGLARVLYSNVIPALLNNpLWHERDLTNSS 318 Pyrococcus horikos...
NP_247924 253 IAQTLNKIGVTVRSMQRteIGELEEEfdptkqTGSSTMPHKRNPITFEQICGLSRVIKSLCIAEMDNIpLWEERDLTNSS 332 Methanocaldococcus...
NP_614368 248 IGSTLDKIGREIRNLQRteIREVEEPfdpekqVGSSTMPHKRNPIRSERVCSLARVLRSNVQIALENVpLEHERDLTNSA 327 Methanopyrus kandl...
NP_618841 242 TVTTLDKIGIEIRNLQRseIAEIEESfgk-kqVGSSTMPHKRNPIKSEQICGLARIVRAMVEPELLNNtLWDERDLTNSS 320 Methanosarcina ace...
NP_071067 236 LAATLEKIALNFRLLQRaeVGELMEKfea-kqVGSSTMPHKRNPIDCENVCGLARVVRGFVEPQHQSAiLWEERDLTNSS 314 Archaeoglobus fulg...
NP_341790 257 LASQCDRFALEVRELMRpeINEISEGve--grVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLENIpLWHERDLTNSS 334 Sulfolobus solfata...
AAT43401  240 IATSLEKFATEIRNLQRpeINEVSEYfdersqVGSSSMPSKRNPIVSENISSLCRFIRSLIIPEYEAAvIWHERDLANSA 319 Picrophilus torrid...
Feature 1                                                                                     
1C3U_A    308 VERYVFPDATQTLYYMIVTATNVVRNMKVNEeRMKKNIDLtkGLVFSQRVLLKLIEKGlTRKEAYDIVQRnalktwnsek 387 Thermotoga maritima
1DOF_A    308 NERVWIPEALLALDEILTSALRVLKNVYIDEeRITENLQKalPYILTEFHMNRMIKEGaSRAEAYKKAKEvka------- 380 Pyrobaculum aeroph...
NP_276650 321 SERIILPEACILTDYILKLTLDVLCNLVFYPeNIKRNLEFtgGLIMAERLMAELTRRGmGRQTAYAAVRQcaieasrtgr 400 Methanothermobacte...
NP_142783 319 VERVILPESFVLLDEMLKTMKKVLTGLEFFPeNIRRNLYLtkNLIMAEPLMLKLAEKGmGRQEAHEVVRQlamkafrenr 398 Pyrococcus horikos...
NP_247924 333 AERCIFAEVCVLTDHILTLAIKGVKKLKVNKeNVERNLELtkGLIMAERIMIELAKRGmGRQTAHEIVRQcamkayeekr 412 Methanocaldococcus...
NP_614368 328 SERVILPEQFLLLDEMLRLTIHNLEGLRVYEeNIRENLRLtkGLNMAEALMVELVKRGiGRQEAHELVRRlamraweegr 407 Methanopyrus kandl...
NP_618841 321 CERVVFPEACVLTDHILKLGIRVLENLRFYPeNIRRNLDLlrGLNMGEAVMIELAKRGvGRQEAHELVRTsameahetgq 400 Methanosarcina ace...
NP_071067 315 AERITLVESTVLADHILTKMIKVVSSVSLNLeNIRRNLEMqrGLNLSEAVMIEMTKRGvGRQEAHEILRQaamrayenns 394 Archaeoglobus fulg...
NP_341790 335 SERIIISHEFLVIDEILDSMISLLRNLRVNFeNIEKNLNLtkGQIMAESLMINLTLKGmKRHEAHQYVNKltskvrqtgk 414 Sulfolobus solfata...
AAT43401  320 LERFTIPYASVLIDYVLYNMNDVFSHLYINKeEMLRKARSd-EFIMSESIVRALTLSGmPRQEAHEFVRKasmeayknnk 398 Picrophilus torrid...
Feature 1                                             
1C3U_A    388 hfleylledeevkklvtkeeleelfdiSYYLKHVDHIFER 427 Thermotoga maritima
1DOF_A    381 ------------------------ltfEYQKWPVERLIED 396 Pyrobaculum aerophilum
NP_276650 401 slrdvvlerseimdyltvedleeimnpETYIGSARRMVER 440 Methanothermobacter thermautotrophicus str. Delta H
NP_142783 399 dllevake-sreitkylterdleelkpENYIGKAREIVKK 437 Pyrococcus horikoshii OT3
NP_247924 413 hlkdilleneevmkyitkeeleelmnpKTYIGLAPQIVDE 452 Methanocaldococcus jannaschii DSM 2661
NP_614368 408 dfaevvkeeervrelfgeeeldevldpEKYLGVAPDLARE 447 Methanopyrus kandleri AV19
NP_618841 401 hfkqvlldtpsvsrylsvedieklvnpDKYIGTAVEQVEA 440 Methanosarcina acetivorans C2A
NP_071067 395 slldellkdervmkyfkedelreilkpENYLGTARERVER 434 Archaeoglobus fulgidus DSM 4304
NP_341790 415 tllevcledslilsyfskqelenvlnpKNYLGSYDKLIER 454 Sulfolobus solfataricus P2
AAT43401  399 slksslis-agvlkyidektleaamdpVKFTGQAVSICNN 437 Picrophilus torridus DSM 9790

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