1K7W,1I0A,1XWO,1HY0,1TJ7,1AOS


Conserved Protein Domain Family
Argininosuccinate_lyase

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cd01359: Argininosuccinate_lyase 
Click on image for an interactive view with Cn3D
Argininosuccinate lyase (argininosuccinase, ASAL).
This group contains ASAL and related proteins. It is a member of the Lyase class I family. Members of this family for the most part catalyze similar beta-elimination reactions in which a C-N or C-O bond is cleaved with the release of fumarate as one of the products. These proteins are active as tetramers. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits. ASAL is a cytosolic enzyme which catalyzes the reversible breakdown of argininosuccinate to arginine and fumarate during arginine biosynthesis. In ureotelic species ASAL also catalyzes a reaction involved in the production of urea. Included in this group are the major soluble avian eye lens proteins from duck, delta 1 and delta 2 crystallin. Of these two isoforms only delta 2 has retained ASAL activity. These crystallins may have evolved by, gene recruitment of ASAL followed by gene duplication. In humans, mutations in ASAL result in the autosomal recessive disorder argininosuccinic aciduria.
Statistics
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PSSM-Id: 176463
View PSSM: cd01359
Aligned: 99 rows
Threshold Bit Score: 344.145
Threshold Setting Gi: 67641634
Created: 14-Jul-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitestetramer
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:active sites [active site]
Evidence:
  • Comment:The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits.
  • Structure:1K7W_A; duck delta 2 crystallin mutant tetramer with bound argininosuccinate, contacts at 4 A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1       #   #                                                           # #             
1K7W_A       29 SIAYDQRL----SEVDIQGSMAYAKALEKAGILTKTELEKILSGLEkiseewskgvfVVKQSDEDIHTANERRLKEli-g 103 Anas platyrhynchos
Q8U705       25 RPLFDGAKnh-hVDGFRRIDRAHLVMLRETGILDAETAAKIAGALEdidrtiepselVYTGEVEDFFFLIEKELKArigv 103 Agrobacterium tu...
ZP_00556714  11 APLFEGVKih-fADHMDRINRAHLVMLVETGIVLREDGEAIAEALAdietnvdrqalDYTGEHEDWFFLVEAELRArl-g 88  Jannaschia sp. CCS1
EAN07049     24 LDRYDSRTvetkLEAVHAFDKAHLVMLVEQGLVGRQDGALMLSALRhmek---egavATRMRVGGALHSGEYYLTErlgp 100 Mesorhizobium sp...
YP_076579    59 YDSAKHEL----LEPMMAIHRAHLVMLVEQGLVDAGTARKILAGLAsidlg-evaasRYDGRYEDLFFYVEDLTLKaa-g 132 Symbiobacterium ...
NP_833283    30 VFDFQRNYl---LKDMFQVHKAHITMLTEENLMKKEEAKVILHALKkvegi-peeqlLYTEQQEDLFFLVEHLISQeakc 105 Bacillus cereus ...
EAN06647     26 LIAQDIEM----FDAYLAVDLAHVIMLADRGIIGPDPARALVGVLSklrsd-gpasiKIDASFGGFLLQVERHIASvsgp 100 Mesorhizobium sp...
EAN06646    205 VLKRELDM----FDAYVRVDLAHITMLAEQGIIPIKVAKALLGALQdlrra-gpqglDVKGELGSILLQVEHYLAErvga 279 Mesorhizobium sp...
NP_885580    29 AAAGHEDL----FHAMSRVNLAHAVMLRDRNLIPPEAARALVALMAelfer-gpssvALEAGAGDLYPQIEAHAAAllga 103 Bordetella parap...
AAV52072     32 EIADAPLL----HEGLGLADLAHVLALTEDGIMPEVASCALTAELLeilat-padefPYDVVYGDAYNSRERELERrl-g 105 Nocardioides sp....
Feature 1                 ###  #                                         ##                     
1K7W_A      104 DIAGKLHTGRSRNDQVVTDLKLFMKNSLSIISTHLLQLIKTLVERAaiEIDVILPGYTHLQKAQPIRWSQFLLSHAVALT 183 Anas platyrhynchos
Q8U705      104 DVAGRLHTARSRNDIDHTLFKIGLKDKIDTLTAKARVLLKALIDAAerNQSTLIVAYTHGQPAQPTTFGHYLSAAIEVLI 183 Agrobacterium tu...
ZP_00556714  89 DLGGALHTARSRNDMDHTMFKMALRDRAENALARMLTLAQALLDKAraERDTLIVAYTHGQPAQPATFGHYLSAVIEVLV 168 Jannaschia sp. CCS1
EAN07049    101 KLGGTIHLGRSSADLAGVSRRIVLRTAVLSMARQLIDVQLTILNTApdHLDCIIPAYTHGQPAQPTTYAHWLMMWANVFH 180 Mesorhizobium sp...
YP_076579   133 EEAGNLHIARSRNDMGVTMYRMVLRKHLLDAIEAAQLLHRVLLRFAaeEADTVMLAHTHTQQAQPTTLGHWLTAAADVLE 212 Symbiobacterium ...
NP_833283   106 DFVSNMHIGRSRNDMGVTMYRMSLRRYVLRLIEQHLLLQESVLQLAddHKETIMPAYTHTQPAQPTTFGHYTLAIYDTMQ 185 Bacillus cereus ...
EAN06647    101 DAAGMLQLGRSRIDQNAAVARVYARNSLISVMNQLVDLQGVVLDRAreWKAVIMPGYTHLQHAQPWVLGHYLLGQHDVFQ 180 Mesorhizobium sp...
EAN06646    280 ESAGYLQLARSRIDQNATISRVHARDELLHVAERIVDFQKVILQRAeeWADVLMPGYTHLQHAQPWILGHYMIGQHDVFA 359 Mesorhizobium sp...
NP_885580   104 DVAGYLQLGRSRGDVIPSAMRLKLRIKLMRLLASVLALREAILDRAveRADTIMPAYTHWQQSQIMTVGHFLGRFSAWLE 183 Bordetella parap...
AAV52072    106 DTAGWLHAGRTRREAGRIAFRIALRRRLLDLHDGVGDLALAFATAArlHADTLWADTTYLQPAQPSTFGHYLGAFAEETT 185 Nocardioides sp....
Feature 1                                                              #                        
1K7W_A      184 RDSERLGEVKkRINVLPLGSGALAGNPLDIDREMLRSELEFaSISLNSMDAISE-RDFVVEFLSFATLLMIHLSKMAEDL 262 Anas platyrhynchos
Q8U705      184 RDIERFTEARhIVDLSPMGAAAITTSGFPIDRARVAELLGFaAPLRNSYSCIAA-VDYTTATYGAIELMFLHLGRLIQDF 262 Agrobacterium tu...
ZP_00556714 169 SDARRLETAItHVDHCPMGAAAITTSGFPIDRERMAELLGFaGVRVNAYGCIAG-VDYVTGLYSALKLPMIHLGRVVQDM 247 Jannaschia sp. CCS1
EAN07049    181 RDTQRLLGGLdRLNLSPAGAAVATGTDFAIDRSRVATLLGFdGILVNTMDATLS-NDVFAEYVSIFSLLCANMGRLAEDL 259 Mesorhizobium sp...
YP_076579   213 RDIARMQAAYeRANRSPMGAAALSTSGFPISRERVAELLGFdGLVENSYDAIAG-ADYVSEAAAAVQLTALHTGRMVQDL 291 Symbiobacterium ...
NP_833283   186 RDLERMKKTYqLLNQSPMGAAALSTTSFPIKRERVAHLLGFtTVIENSYDAVAG-ADYLLEVSSLLMVMMTNTSRWIHDF 264 Bacillus cereus ...
EAN06647    181 RDFQRLVGVYgRTNLGSLGGVALSGTSWPIDRRYTAELLGHaGIVRNSKDAGNFvMDHQPELAAMLSILMSALGRLASEL 260 Mesorhizobium sp...
EAN06646    360 RDLQRVIQTYdRVNLSSLGGLALVGTSWPIDRERTAFLLGHsAPIRHSKDCAGFaMDYLAEVGSSLSILMSGLGRLASEW 439 Mesorhizobium sp...
NP_885580   184 RDTQRLFECVsRHNLSVLGSANGVGTSVRVDRAMTAAWLGHdEPTLSTGDGTNA-WDYMIEPVSVAALLCSHLARLTGNF 262 Bordetella parap...
AAV52072    186 RHLSRIQSAYdLANRSPTGSGGVGGSPLLVNRVRDAVALGFdEPGWHTRDVMWS-VDGLVDAVFCSAQAVTTVDRLAEDL 264 Nocardioides sp....
Feature 1                         # #       # #  #                           # #  # ##          
1K7W_A      263 IIYSTseFGFLTLSDAFSTGASLMPQKKNPDSLELIRSKAGRVFGRLASILMVlkgl-pstYNKDLQEDKEAVFDVVDTL 341 Anas platyrhynchos
Q8U705      263 QFWTSfeVGQIYVPNSLVQISSIMPQKRNPVPIEHLRHLASQTFGRARTMLDVmhntpftdMNDSEGETQSMGYEAFASA 342 Agrobacterium tu...
ZP_00556714 248 AFWSAfeVGQLYVPRALVQISSIMPQKRNPVPIEHLRHLASVAAGRCDAMINTmhntpftdMNDSEGEVQQAGYGAFDHA 327 Jannaschia sp. CCS1
EAN07049    260 LFWSTqeAGIAVIPDRFCDTSSIMSQKRNPSFLHELRAMGAEAASTLLLVSGTefgt-tgqSVHARKTAENAVWALVGKI 338 Mesorhizobium sp...
YP_076579   292 LLWCTqeYGALRVADPYVQTSSIMPQKRNPVSLEHARTLLSYTAGRAQTVLQMlhntpfgdIVDTEDEMQPNLWAALSTL 371 Symbiobacterium ...
NP_833283   265 LLLATkeYDGITVAKPYVQISSIMPQKRNPVSIEHARAITSSALGEAFTVFQMihntpfgdIVDTEDDLQPYLYKGIEKA 344 Bacillus cereus ...
EAN06647    261 YIWSSweFGLVELDESLCGTSSIMPQKKNPYALERIRALAGEAIGWLPGQLGLfkt---ptTSDCDRSFSSGITPCFKAT 337 Mesorhizobium sp...
EAN06646    440 YIWSTweFNLIELDEGLCGTSSIMPQKKNPYVLERVRALAGESIGWTAAQLGMlrt---ptTVDADRFFSTGNVEYFRVA 516 Mesorhizobium sp...
NP_885580   263 ILWHTqeFGMVRLSDAFCTASTYLPHKRNPEPLETVKVFAEQVQGALATVYLMarn--edwPHSLINHGFDAINGALTLS 340 Bordetella parap...
AAV52072    265 EIFASpaFGYVRLDASLCRASVLLPQKRNPYALAVIRSGANVLVGRAAGMLATqrt--psaRTDNWLHAYGEVAGSVDLA 342 Nocardioides sp....
Feature 1                                                                                       
1K7W_A      342 TAVLQVATGVISTLQISKENMekal-tpeMLATDLALYLVR-KGVPFRQAHTASGKAVHLAetkgitinklsledlk--- 416 Anas platyrhynchos
Q8U705      343 GRVLDLLASLVGQISIDPERVdqnirrscITITELADSLVRiEDLSFRQAHEIAATVAKSVvalkgdlpndgyqpflgaf 422 Agrobacterium tu...
ZP_00556714 328 TRVFDLLTAFLPACSINADRVaqttdaacITITELADTLVRdEGLSFRLAHDIASQTARAViadarplgggyaafsqafe 407 Jannaschia sp. CCS1
EAN07049    339 IKRLPQMADLIASTEIDPQRAmrltenswSQATDVASALVRhLGLNWREAHHVVSILVRLAterrlppdqvdlallrtal 418 Mesorhizobium sp...
YP_076579   372 RSVFRLLANVVGTAEVNRDLLlerarasfANVTELADLLVReAGLPFRTAHGVVAALVKAAeargirdvrdvdpgmveea 451 Symbiobacterium ...
NP_833283   345 IRVFCMMNAVILTMKVEEETLksrsykhaITITDFADVLTKnYEIPFRHAHHAASAIANMSleqkkelhelhfkdvnmyl 424 Bacillus cereus ...
EAN06647    338 RWGLLLMSEALRTMRIDENRMreragafwSTASNLADLIVReRGLDFRRAHHVVGALVKTAvadglrpsdvgaaevdaaa 417 Mesorhizobium sp...
EAN06646    517 NWALDLMQDCAATVRINRERMrelaganwNTASNLADAIVReCGLDFRSAHQIVGALVKASiesgthsrdvglaevnaaa 596 Mesorhizobium sp...
NP_885580   341 GDAADLTGAIMRDISFDPARMrelldlsyATASEVANTLVAaGGLPFREAHEIVGTAVRACmadgraaltparvrdaarg 420 Bordetella parap...
AAV52072    343 SRLVRLGTAVVSSLEVDRDALtreaaahfTGAADLAESIMVnLGLDYRSAYRVVGHAVGVAidagrtallasdvdlaak- 421 Nocardioides sp....
Feature 1                                                          
1K7W_A      417 ------sispqfssdvsqvfnFVNSVEQYtALGGTakSSVTTQIEQLRELM 461 Anas platyrhynchos
Q8U705      423 s-gltgretgideekfrqivsPEHFVAVRsRFGGPapEPMREAFAAYRGKL 472 Agrobacterium tumefaciens str. C58
ZP_00556714 408 --aatgkppklnaaefaeavdARTFVSRRdRTGGPapAALDAAFEIYSSDI 456 Jannaschia sp. CCS1
EAN07049    419 a-eaqiaaedldgfdiadavdVRAFVERRtNEGSPapSAVTSSAACLLGIT 468 Mesorhizobium sp. BNC1
YP_076579   452 aqkvlgrplgvpaeklrlaldPVHFVAVRtVRGGVapAEVRRMLAEREARA 502 Symbiobacterium thermophilum IAM 14863
NP_833283   425 ---qehfkinvlekewkeiisPEAFIQKRnVYGGPskKEMKRMIKNRKESF 472 Bacillus cereus ATCC 14579
EAN06647    418 k-avlsrsldftdeqistalnSAEFVATRvSEGSIgpQEIEKQLNLAMEDH 467 Mesorhizobium sp. BNC1
EAN06646    597 r-atigreismtdqqihdsldPEQFVATRvSQGSLskAELQKLFDMARTDI 646 Mesorhizobium sp. BNC1
NP_885580   421 ---mgidlppiddaqlaacidPGNFVQAIhSSGGTapAEVRRVLAVWRDAL 468 Bordetella parapertussis 12822
AAV52072    422 ---ellgealsprpdvesitdPGRIVATRdVAGGSaaGRVREHALRVEGLA 469 Nocardioides sp. JS614

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