1AMI,1L5J


Conserved Protein Domain Family
Aconitase

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cd01351: Aconitase 
Click on image for an interactive view with Cn3D
Aconitase catalytic domain; Aconitase catalyzes the reversible isomerization of citrate and isocitrate as part of the TCA cycle
Aconitase catalytic domain. Aconitase (aconitate hydratase) catalyzes the reversible isomerization of citrate and isocitrate as part of the TCA cycle. Cis-aconitate is formed as an intermediate product during the course of the reaction. In eukaryotes two isozymes of aconitase are known to exist: one found in the mitochondrial matrix and the other found in the cytoplasm. Aconitase, in its active form, contains a 4Fe-4S iron-sulfur cluster; three cysteine residues have been shown to be ligands of the 4Fe-4S cluster. This is the Aconitase core domain, including structural domains 1, 2 and 3, which binds the Fe-S cluster. The aconitase family also contains the following proteins: - Iron-responsive element binding protein (IRE-BP), a cytosolic protein that binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5'UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'UTR of transferrin receptor mRNA. IRE-BP also express aconitase activity. - 3-isopropylmalate dehydratase (isopropylmalate isomerase), the enzyme that catalyzes the second step in the biosynthesis of leucine. - Homoaconitase (homoaconitate hydratase), an enzyme that participates in the alpha-aminoadipate pathway of lysine biosynthesis and that converts cis-homoaconitate into homoisocitric acid.
Statistics
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PSSM-Id: 153129
View PSSM: cd01351
Aligned: 7 rows
Threshold Bit Score: 225.839
Threshold Setting Gi: 14521812
Created: 12-Dec-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
ligand bindingsubstrate
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1AMI binding Fe-S cluster; defined by contacts at 3.5A
    View structure with Cn3D
  • Structure:1L5J_A binding Fe-S cluster; defined by contacts at 3.5A
    View structure with Cn3D
  • Citation:PMID 3972791

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1AMI         68 RVAMQDATAQMAMLQFISsglp---------kvavPSTIHCDHlieaqlggek--------------------------- 111  cow
1L5J_A      410 SVGSQDTTGPMTRDELKDlaclg--------fsadLVMQSFCHtaayp-------------------------------- 449  Escherichia coli
P48200       84 RVLLQDFTGIPAMVDFAAmreavktlggdpekvhpACPTDLTVdhslqidfskcaiqnapnpgggdlqkagklsplkvqp 163  human
NP_593437    93 HCMSHDNSWPVALKFMGIgakk----------vfdNRQIVCTLdhdvqn------------------------------- 131  fission yeast
O28316       30 LAMIHDITAPLAIKAFREilgsd-------akvwdKSKVIMAFdhqvpa------------------------------- 71   Archaeoglobus f...
ZP_00188258  31 QTLLQDATGTMACLQFERmgvd---------rvrvPFAVQYVDhniiql------------------------------- 70   Rubrobacter xyl...
NP_127288    10 ELILSGEYGPALQKAMEIlvalgdlygadklipikSAQIAGVSyk----------------------------------- 54   Pyrococcus abys...
Feature 1                                                                                       
1AMI        112 -----------------------------------------------------dlrrakdinqEVYNFLATAGAKyg-vG 137  cow
1L5J_A      450 ----------------------------------------------------------kpvdvNTHHTLPDFIMNrg-gV 470  Escherichia coli
P48200      164 kklpcrgqttcrgscdsgelgrnsgtfssqientpilcpfhlqpvpepetvlknqevefgrnrERLQFFKWSSRVlknvA 243  human
NP_593437   132 ---------------------------------------------------------kseanlRKYKNIESFAKGqg-iD 153  fission yeast
O28316       72 ---------------------------------------------------------dsvhaaENHKMLRKFAEEqg-iL 93   Archaeoglobus f...
ZP_00188258  71 ----------------------------------------------------------dhknpDDHRFLQAFAARyg-iH 91   Rubrobacter xyl...
NP_127288    55 --------------------------------------------------------------nLGEAGLSFIRDLaemgA 72   Pyrococcus abys...
Feature 1                                                                           #           
1AMI        138 FWRP------------------------------GSGIIHQIILEny---------ayPGVLLIGTDSHTPnggglGGIC 178  cow
1L5J_A      471 SLRP------------------------------GDGVIHSWLNRml----------lPDTVGTGGDSHTRf---pIGIS 507  Escherichia coli
P48200      244 VIPP------------------------------GTGMAHQINLEylsrvvfeekdllFPDSVVGTDSHITmvnglGILG 293  human
NP_593437   154 FYPA------------------------------GRGIGHQIMVEqgy--------amPGSMAVASDSHSNtyggvGCLG 195  fission yeast
O28316       94 NYDV------------------------------KGGIAHQIMVEnh---------vePGMLIVGADSHTCmygalGAFA 134  Archaeoglobus f...
ZP_00188258  92 YSRP------------------------------GNGICHYLHVErf---------arPGATLIGADSHTTssgalGSIA 132  Rubrobacter xyl...
NP_127288    73 KVSVyttlnppgigdeefmekqrevieiyrkmgvDVTSTCTPYYGanl--------pkFGDHLAWSESSAVs----FANS 140  Pyrococcus abys...
Feature 1                                                                                       
1AMI        179 IGVG----GADAVDVmagipWELKCPKVIGVKLTGSlsg------------wtSPKDVILKVAGiltvk----------- 231  cow
1L5J_A      508 FPAG----SGLVAFAaatgvMPLDMPESVLVRFKGKmqp------------giTLRDLVHAIPLyaikqglltvekkgkk 571  Escherichia coli
P48200      294 WGVG----GIETEAVmlglpVSLTLPEVVGCELTGSsnp------------fvTSIDVVLGITKhlrqv----------- 346  human
NP_593437   196 TPIV----RTDAAAIwatgqTWWQIPPIARVNLVGQlpk------------glSGKDIIVSLCGafnhd----------- 248  fission yeast
O28316      135 TGIG----STDMGFVlamgkLWFKVPESIRFNVHGKlek------------hvYGKDIVLKLIGmvgad----------- 187  Archaeoglobus f...
ZP_00188258 133 IGAG----GLDVALVmagqpFQTPAPKVVGVELTGElsd------------wvTPKDVILELLRrrgvr----------- 185  Rubrobacter xyl...
NP_127288   141 VLGArtnrEGGPSSLa---aAIVGKTPNYGLHLEENrkathivtvkaelkdetDYSLLGYKVGEiv-------------- 203  Pyrococcus abys...
Feature 1                                                                                       
1AMI        232 -ggtgAIVEYHGpgvdsISCTGMATICNMG-----AEIGATTSVFPYNhrmkkylsktgradian--------------- 290  cow
1L5J_A      572 nifsgRILEIEGlp--dLKVEQAFELTDAS-----AERSAAGCTIKLNkepiieylnsnivllkwmiaegygdrrtlerr 644  Escherichia coli
P48200      347 -gvagKFVEFFGsgvsqLSIVDRTTIANMC-----PEYGAILSFFPVDnvtlkhlehtgfskaklesme----------- 409  human
NP_593437   249 -evlnHAIEFYGeglnsLSIESRLTIANMT-----TEWGALSGLFPTDekllawyedrlkflgpnhprv----------- 311  fission yeast
O28316      188 -ganyKACIYSGevvekLGMSDRLTMCNMA-----IEMGGKAGIVEPDkttleylkamgrp------------------- 242  Archaeoglobus f...
ZP_00188258 186 -gglnRIFEFYGegvatIDVTGRTTICNMI-----AELGATTGIFPSDertrewleaqqrpd------------------ 241  Rubrobacter xyl...
NP_127288   204 ---enGIPYFKNl---sPSTDELKALGASMaasggVALYHVEEHTPEWrts----------------------------- 248  Pyrococcus abys...
Feature 1                                                                                       
1AMI        291 -----ladefkdhlvpdsgchyDQLIEINLse-lkPHINGPFTP-DLAHPVaevgsvaek-------------------- 343  cow
1L5J_A      645 iqgmekwlanpelleadadaeyAAVIDIDLadikePILCAPNDP-DDARPLsavqg------------------------ 699  Escherichia coli
P48200      410 -tylkavklfrndqnssgepeySQVIQINLns-ivPSVSGPKRP-QDRVAVtdmksdfqaclnekvgfkgfqiaaekqkd 486  human
NP_593437   312 -nretldaikaspiladegafyAKHLILDLst-lsPAVSGPNSV-KVYNSAatlekk----------------------- 365  fission yeast
O28316      243 ---------yegellksdedaeFQEVELDVtg-mePQVAAPHRV-DNVVGIseveg------------------------ 287  Archaeoglobus f...
ZP_00188258 242 ---------dfvelaadegasyDEHEHIALde-lePLIAKPHSP-GNVVKVeevag------------------------ 286  Rubrobacter xyl...
NP_127288   249 --------------------ivDKVEKVEIe---dKDIKEVQENfNAEWSEvd--------------------------- 278  Pyrococcus abys...
Feature 1                                #                                                      
1AMI        344 -----------egwpldirvGLIGsCTNss-yeDMGRSAAVAkqalah--glkckSQFTITPGSe---QIRATIEr---- 402  cow
1L5J_A      700 ---------------ekideVFIGsCMTni--gHFRAAGKLLdahk-----gqlpTRLWVAPPTr---MDAAQLTe---- 750  Escherichia coli
P48200      487 ivsihyegseyklshgsvviAAVIsCTNncnpsVMLAAGLLAkkaveaglrvkpyIRTSLSPGSg---MVTHYLSs---- 559  human
NP_593437   366 --------------dilikkAYLVsCTNgr-lsDIHDAAETVkgkk-----vadgVEFYVGAASs---EVEAAAQk---- 418  fission yeast
O28316      288 ---------------trvdqVFIGsCTNgr-yeDLKIAAEILkgek-----vasnVRLIVIPASh---REYRRALk---- 339  Archaeoglobus f...
ZP_00188258 287 ---------------tkaaqVCIGsSVNsg-fdDLAVAAAVLrdqi-----vypgLVMTVTPGSr---QILDSIAr---- 338  Rubrobacter xyl...
NP_127288   279 -------------------fILLG-CPH-----ASLKEVKKI-------------ADVIRMRETplkvPLYVTVSrgvka 320  Pyrococcus abys...
Feature 1                             #  ##                           #                         
1AMI        403 ----DGYAQVLRDVGGIVLaNACGPCIGqwdrkd-------ikkgekNTIVTSYNRNFTGRNDanpethafVTSPEIVTA 471  cow
1L5J_A      751 ----EGYYSVFGKSGARIEiPGCSLCMGnqar-----------vadgATVVSTSTRNFPNRLGtga--nvfLASAELAAV 813  Escherichia coli
P48200      560 ----SGVLPYLSKLGFEIVgYGCSTCVGntaplsdavlnavkqgdlvTCGILSGNKNFEGRLCdcvr-anyLASPPLVVA 634  human
NP_593437   419 ----NGDWQTLIDSGARTLpAGCGPCIGlgtgl----------lkdgEVGISATNRNFKGRMGsrea-layLASPAVVAA 483  fission yeast
O28316      340 ----EGLIEIFVDAGALVEaPCCGPCMGgsfgl----------iasgEVSVSTSNRNFIGRQGspeg-kiyLVNPAVAAA 404  Archaeoglobus f...
ZP_00188258 339 ----TGVYSDLVLAGARMLePVCGPCVGmgqa-----------ppsdSVSVRTFNRNFPGRSGtmnd-hvyLTTPTVAAA 402  Rubrobacter xyl...
NP_127288   321 lsdfLGYTEIIERYNGRLIpDACLVVSP-------------------IKKWYSGVATNSGKAAfyf--rsfGLKVKLDKL 379  Pyrococcus abys...
Feature 1              
1AMI        472 LAIAGTL 478  cow
1L5J_A      814 AALIGKL 820  Escherichia coli
P48200      635 YAIAGTV 641  human
NP_593437   484 SAIAGKI 490  fission yeast
O28316      405 TAIYGEI 411  Archaeoglobus fulgidus
ZP_00188258 403 TALKGVI 409  Rubrobacter xylanophilus DSM 9941
NP_127288   380 ENLILGA 386  Pyrococcus abyssi GE5

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