1GUZ,1GUY,1GV0,1HYE,1LDG,1OJU,1PZH,2D4A,2FM3,2HJR,1SOV,1OC4,2A92,1HLP


Conserved Protein Domain Family
LDH-like_MDH

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cd01339: LDH-like_MDH 
Click on image for an interactive view with Cn3D
L-lactate dehydrogenase-like malate dehydrogenase proteins
Members of this subfamily have an LDH-like structure and an MDH enzymatic activity. Some members, like MJ0490 from Methanococcus jannaschii, exhibit both MDH and LDH activities. Tetrameric MDHs, including those from phototrophic bacteria, are more similar to LDHs than to other MDHs. LDH catalyzes the last step of glycolysis in which pyruvate is converted to L-lactate. MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. The LDH-like MDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133424
View PSSM: cd01339
Aligned: 70 rows
Threshold Bit Score: 328.664
Threshold Setting Gi: 999809
Created: 30-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:1GUZ_A: Prosthecochloris vibrioformis malate dehydrogenase, contacts to NAD at 3.5A
    View structure with Cn3D
  • Structure:1LDG_A: Plasmodium Falciparum L-Lactate Dehydrogenase contacts to NAD, determined at 3.5A
    View structure with Cn3D
  • Citation:PMID 8901865
  • Structure:1HYE_A; Methanocaldococcus jannaschii Mj0490 contacts to NADP at 3.5A
    View structure with Cn3D
  • Comment:The Methanocaldococcus jannaschii Mj0490 prefers NADP(H) as a cofactor, unlike related LDH-like MDH family members which prefer NAD(H).

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               ###                    ##                                                
1GUZ_A         3 ITVIGA-GNVGATTAFRLAEKQLArELVLLDVV-------EGIPQGKALDMYESGpVGLFDTKVTGSNd------YADTA 68  Prosthecochlori...
1OJU_A         3 LGFVGA-GRVGSTSAFTCLLNLDVdEIALVDIA-------EDLAVGEAMDLAHAAaGIDKYPKIVGGAd------YSLLK 68  Archaeoglobus f...
2D4A_A         2 ITILGA-GKVGMATAVMLMMRGYD-DLLLIART-------PGKPQGEALDLAHAAaELGVDIRISGSNs------YEDMR 66  Aeropyrum pernix
1HLP_A         3 VSVVGAaGTVGAAAGYNIALRDIAdEVVFVDIPd-----kEDDTVGQAADTNHGI-AYDSNTRVRQGG-------YEDTA 69  Haloarcula mari...
O67655         7 VAVIGA-GNVGEHVASLILLKNLA-NVKMFDLPrkteekvFEPVKGKALDMKQMLaAMDIDARVEGYTvtpegegYEPLE 84  Aquifex aeolicus
Q7VFV4         5 IAIIGGsGNVGSHIAFLGAMRHIAkEILLFSND-------IPRCKGVGLDISQAAaIFDIPILIKGCNs------YEEIA 71  Helicobacter he...
Q8ZVB2         2 ITIIGS-GRVGTAAAVIMGLMKLDnKILLIDIV-------KGLPQGEALDMNHMSsILGLDVEYVGSNe------YKDIE 67  Pyrobaculum aer...
A8MAC1         2 ITIVGS-GRVGATTAAFLMFYELDnEVTLIDVI-------KGLPQGEALDLNHAAaILGKSVRYKGSNd------YKDME 67  Caldivirga maqu...
YP_876499      2 ITIIGA-GKVGGDAAMFCALRRLDsEILLLDIV-------EGLPQGEAMDINHMLaEQGIDTEVRGSNd------YSDME 67  Cenarchaeum sym...
YP_001930815   9 VSIVGA-GNVGEHVANLIAIKELA-NIKLFDLPkktedktFEVVKGKALDIKQMAaALGVDVDIKGYNvtpdgqgYEPMK 86  Sulfurihydrogen...
Feature 1               ####                 #                   # #                      #   #  
1GUZ_A        69 NSDIVIITAGLPRKPGMTREDLLMKNAGIVKEVTDNIMKHskNPIIIVVSNPLDIMTHVAWVRSGLPKERVIGMAGVLDA 148 Prosthecochlori...
1OJU_A        69 GSEIIVVTAGLARKPGMTRLDLAHKNAGIIKDIAKKIVENapESKILVVTNPMDVMTYIMWKESGKPRNEVFGMGNQLDS 148 Archaeoglobus f...
2D4A_A        67 GSDIVLVTAGIGRKPGMTREQLLEANANTMADLAEKIKAYakDAIVVITTNPVDAMTYVMYKKTGFPRERVIGFSGILDS 146 Aeropyrum pernix
1HLP_A        70 GSDVVVITAGIPRQPGQTRIDLAGDNAPIMEDIQSSLDEHndDYISLTTSNPVDLLNRHLYEAGDRSREQVIGFGGRLDS 149 Haloarcula mari...
O67655        85 GSDIVVITAGFPRRPGMSREDLLEANIRIISVIADRIKRYapDAIVIVVTNPVDVMTYVAYKLLNFPKNRVMGMAGVLDS 164 Aquifex aeolicus
Q7VFV4        72 ESEVVIITAGFPRTPNMTRNDLLLKNASIIQEISSNVARIapQSLLIVVSNPLDAMCLVAKQWSKFEKERVIGMAGILDS 151 Helicobacter he...
Q8ZVB2        68 GSDLIIVTAGLPRKPGMTREQLLEANAKIVAEIGREIKKYapDSIVILTTNPLDAMTYVMWKATGFPRERVIGFSGVLDA 147 Pyrobaculum aer...
A8MAC1        68 GSDIVIVTAGLARKPGMTREELAGKNAEIISSIADQIKKYapNSIVIITTNPLDAMVYVLYKRLGFPRNRVIGFSGVLDS 147 Caldivirga maqu...
YP_876499     68 GSDIVVVVAGAGRKPGMTRMDLLKINAGIVKGVVEKVKEHakDSMIIPVTNPLDPITYIAYKTSGFEKNRVFGMGGMLDL 147 Cenarchaeum sym...
YP_001930815  87 DSDIVVITAGFPRRPGMSRDDLLTANVNIIRTVAERIALYapNAVVIVVSNPVDVLTYTTLKITGFNKHRVLGMAGVLDT 166 Sulfurihydrogen...
Feature 1                                 #                                                      
1GUZ_A       149 ARFRSFIAMELGvsmqDINACVLGGHGDAMVPVVKYTTVAGIPIsdllpaetIDKLVERTRNGGAEIVEHLkqGSAFYAP 228 Prosthecochlori...
1OJU_A       149 QRLKERLYNAGAr--nIRRAWIIGEHGDSMFVAKSLADFDGEVD--------WEAVENDVRFVAAEVIKRK--GATIFGP 216 Archaeoglobus f...
2D4A_A       147 ARMAYYISQKLGvsfkSVNAIVLGMHGQKMFPVPRLSSVGGVPLehlmskeeIEEVVSETVNAGAKITELRg-YSSNYGP 225 Aeropyrum pernix
1HLP_A       150 ARFRYVLSEEFDapvqNVEGTILGEHGDAQVPVFSKVRVDGTDPefs--gdeKEQLLGDLQESAMDVIERK--GATEWGP 225 Haloarcula mari...
O67655       165 ARFKTFISEELMvspkDIHAYVIGGHGDEMVPLISISNVGGIPLkdllpkekLEKIIERTRFGGGEIVNLMg-TSAYYAP 243 Aquifex aeolicus
Q7VFV4       152 ARLTYESKVMLGdfnkHIQSYVIGSHSDDMLPLLRHCLCEGKVFtdiftpkmQEELIKETKGGGAKIVNYYqkGSAYFAP 231 Helicobacter he...
Q8ZVB2       148 GRLAFYAAKKLGispaSILPIVLGQHGESMFPVPSKSYVHGVPLskllteeqLKEVVEETVKAGARITELRg-FSSNWGP 226 Pyrobaculum aer...
A8MAC1       148 NRMAYYASQIIGiapeSIIPVVLGQHGENMYPVPEASFVYGKPLtefltqeqYNDIVKKTIQAGADITNLRg-FSSNWGP 226 Caldivirga maqu...
YP_876499    148 SRFRQFIHEATGysrdSIRALVMGEHGENMLPLPRFSTVSGIPLlslisaekAAELVRDTKKVAAKVIELK--GATVHAP 225 Cenarchaeum sym...
YP_001930815 167 ARFKTFISQELKvsvsNINCFVLGSHGDDMVPLISVSNVGGIPLkklfteekLKELIDRTKYGGGEIVNLMg-TSAYHAP 245 Sulfurihydrogen...
Feature 1                                                                                       
1GUZ_A       229 ASSVVEMVESIVLdRKRVLPCAVGLEGQY----GIDKTFVGVPVKLGRNGVEqIYEINLDQADLDLLQKSAKIVDENCK 303 Prosthecochloris...
1OJU_A       217 AVAIYRMVKAVVEdTGEIIPTSMILQGEY----GIENVAVGVPAKLGKNGAE-VADIKLSDEEIEKLRNSAKILRERLE 290 Archaeoglobus fu...
2D4A_A       226 AAGLVLTVEAIKRdSKRIYPYSLYLQGEY----GYNDIVAEVPAVIGKSGIErIIELPLTEDEKRKFDEAVQAVKKLVE 300 Aeropyrum pernix
1HLP_A       226 ARGVAHMVEAILHdTGEVLPASVKLEGEF----GHEDTAFGVPVRLGSNGVEeIVEWDLDDYEQDLMADAAEKLSDQYD 300 Haloarcula maris...
O67655       244 AAAIVDMIEALVQnSKRILPCSVYLDGEAgeyyGVQGFCVGVPVKLGSNGVEeIIKVPMIEEEREMWRRSVESVKKTVE 322 Aquifex aeolicus
Q7VFV4       232 ASGVIKILEAISTlNEEILVCSVFTEGEY----GIKDIYLGLPIKLGKKGVEhIVELPLNQQEQEMLNISTQGIKEQVE 306 Helicobacter hep...
Q8ZVB2       227 GAGLAIMAEAVKRdAKRALIASVVLQGEY----GVRDVPVEVPIILGRSGVVkVLEVELTEEERQKFMQSVEAVKKLVA 301 Pyrobaculum aero...
A8MAC1       227 AAGLALMVDSIKKnRRRVFEASVYLDGEY----GVKDVFAEVPVVLGKNGVEkIIELNLTPEQRQKFMQSIEAVKKNLT 301 Caldivirga maqui...
YP_876499    226 GNSISAMVESIVRdKKQVIPVSAYLEGEY----GHSGVTIGVPAVIGRKGVEkIVELDLDGAEKEAFDKGVASVRDAVS 300 Cenarchaeum symb...
YP_001930815 246 GASVVEMVEAILKdKKEIMPCSIYLEGEDarfyEAYDICIGLPVKVGAHGWEeVLKVELSQEEKAMWESSVKSVKSGIE 324 Sulfurihydrogeni...

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