5MDH


Conserved Protein Domain Family
MDH_cytoplasmic_cytosolic

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cd01336: MDH_cytoplasmic_cytosolic 
Click on image for an interactive view with Cn3D
Cytoplasmic and cytosolic Malate dehydrogenases
MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. Members of this subfamily are eukaryotic MDHs localized to the cytoplasm and cytosol. MDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133421
View PSSM: cd01336
Aligned: 28 rows
Threshold Bit Score: 524.112
Threshold Setting Gi: 78192396
Created: 30-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                # ####                         #                                        
5MDH_A         2 EPIRVLVTGAAGQIAYSLLYSIGNGSVFgKDQPIILVLLDITPmMGVLDGVLMELQDCALPLLKDVIATDKEEIAFKDLD 81  pig
XP_001778242   4 DPVRVLVTGAAGQIGYALVPMIARGIMLgPDQPVILHMLDIPPaTESLNGVKMELTDAAFPLLKGVVATTDVAEACKDVN 83  Physcomitrella ...
ABB30235       2 SAVKVAVTGAAGQIGYALVPLIARGILLgPTTHVELRLLDIEPaLKALAGVEAELDDCAFPLLDKVVITADPRVAFDGVS 81  Leishmania mexi...
P93819         4 EPVRVLVTGAAGQIGYALVPMIARGIMLgADQPVILHMLDIPPaAEALNGVKMELIDAAFPLLKGVVATTDAVEGCTGVN 83  thale cress
AAK83037       4 TCKRVAVTGAAGQIGYSLLPLIAAGRMLgFDQRVQLQLLDISPaLKALEGIRAELMDCSFPLLDGVVITDEPKVAFDKAD 83  Trypanosoma brucei
ABK24750      52 EPARVLVTGAAGQIGYALVPMIARGIMLgPDQPVILHMLDIPPaAEALNGVKMELIDAAFPLLKGIVATTDPVEACSGVN 131 Sitka spruce
XP_001014922  44 DEINVCVTGAAGQIGYAFLPLLLTGQCF-GDKKINLRLLDVPQaESILQGVELELQDGAYPLLKSIKTGSNESILFQDVD 122 Tetrahymena the...
CAC80842      10 RPINVCVTGAAGQIAYSLLPLIAGGKVFgPQQQVSLRLLEIEAaLPSLQGVVMELEDCAFPLLHSVFTTSDAREAFRSCD 89  Galdieria sulph...
XP_001434590  25 PPVRVTVTGAAGNIGYALVHMIGQGRLLgPNQQIILTLLELPMaKDQLEGTMMELRDCAFPILKEIRGTTQYDQGFMGCE 104 Paramecium tetr...
XP_001015159   5 AVINVCITGAAGNIAYALYSILCTGQIFgSNQKMNLKLLDIESqAKGLQGVALELEDCAYALVNSIEYGFDPEVLFKDMD 84  Tetrahymena the...
Feature 1            ####                 #                    # #                        #      
5MDH_A        82 VAILVGSMPRRDGMERKDLLKANVKIFKCQGAALDKYAKkSVKVIVVGNPANTNCLTASKSAPs-iPKENFSCLTRLDHN 160 pig
XP_001778242  84 IAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAkDVKVLVVANPANTNALILKEFAPs-iPEKNITCLTRLDHN 162 Physcomitrella ...
ABB30235      82 ISIMCGAFPRRAGMERKDLLEMNARIFKEQGEAIAAVAApDCRVLVVGNPANTNALILLKSAQgklNPRHVTAMTRLDHN 161 Leishmania mexi...
P93819        84 VAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAApNCKVLVVANPANTNALILKEFAPs-iPEKNISCLTRLDHN 162 thale cress
AAK83037      84 IAILCGAFPRKPGMERRDLLQTNAKIFSEQGRVLGEVASpNCRVCVVGNPANTNALILLRESKgklNPRFVTALTRLDHN 163 Trypanosoma brucei
ABK24750     132 IAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEQHAApNCKVLVVANPANTNALILKEFAPs-iPEKNITCLTRLDHN 210 Sitka spruce
XP_001014922 123 VAVFIGGFPRKPGMERKDLLTINGNIFKKQGQALDTVAKkTCKSLVVANPANTNCLILAETAKs-iPKQNFSALTRLDHN 201 Tetrahymena the...
CAC80842      90 VAVLLGAFPRKQGMERKDLLEKNAGIFKEQGEALNTEAGaNVRVIVVGNPANTNAYILKHFAPn-iPDKNITALTRLDQN 168 Galdieria sulph...
XP_001434590 105 IAILVGAKPRGPGMERKDLLAANARIFKEQGEALEKYASkNVKVLVVGNPANTNALITAQFAPs-iPKSNFTALTRLDQN 183 Paramecium tetr...
XP_001015159  85 VGIFLGGQSRRPGMERVDLLQVNNRIFKIQGKALNKVAKpSTKILVIANPVNTNCLTLIQNCPn-iPKENFTCLTRLDHN 163 Tetrahymena the...
Feature 1                                 #                                                     #
5MDH_A       161 RAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVklqakEVGVYEAVKdDSWLKGEFITTVQQRGAAVIKARKLS 240 pig
XP_001778242 163 RALGQVAERLNVPVSTVKNVIIWGNHSSSQYPDVNHAVIdspdgEKAVRDAVSdDAWLDGEFIKVVQQRGAAIIKARKLS 242 Physcomitrella ...
ABB30235     162 RALSLLARKAGVPVSQVRNVIIWGNHSSTQVPDIDNAVIg----TTPAREAIK-DGALNDDFVQLVRERGAEIIQLRGLS 236 Leishmania mexi...
P93819       163 RALGQISERLSVPVSDVKNVIIWGNHSSSQYPDVNHAKVqtssgEKPVRELVKdDAWLDGEFISTVQQRGAAIIKARKLS 242 thale cress
AAK83037     164 RATAQVAERARARVEEVKNCIIWGNHSGTQVPDVNSATVg----GKPARAAVDnDAFFDNEFITIVQERGAEIMKLRGLS 239 Trypanosoma brucei
ABK24750     211 RALGQISERLQVPVSDVKNVIIWGNHSSSQYPDVYHASVvtgagEKAVRQLVAdDAWLDGEFITTVQQRGAAIIKARKLS 290 Sitka spruce
XP_001014922 202 RAISQIALKAGCSITDVKNVIIWGNHSTTQYPDVNHGTVl----GKRIRQFINdEAYLNNAFIERVQKRGGEVLAARKNS 277 Tetrahymena the...
CAC80842     169 RAVSLVARKLGVPLSKVSDVYVWGNHSSTQYPDVLHGSVe----GKGKLSYLLdKSYLEETFIPTIQKRGAAVIAARKMS 244 Galdieria sulph...
XP_001434590 184 RAQSIIAQRVSANVEDVRNIIIWGNHSTTQFADVSQATVqqngiSQTVRGLVAdDAWLQKAFVEQVAKRGGAIIEKRKAS 263 Paramecium tetr...
XP_001015159 164 RAIAQLAIKAKVPVTEVKNVILWGNHSLTMYPDHNHAHIe----GQPAVDIYE-EEWIKNTFIPKVQKRGGEVLNLRQNS 238 Tetrahymena the...
Feature 1                                                                                        
5MDH_A       241 SAMSAAKAICDHVRDIWFGTpeGEFVSMGIISDGNsYGVPDDLLYSFPVTIKDKT-WKIVEGLPINdFSREKMDLTAKEL 319 pig
XP_001778242 243 SALSAASSACDHIRDWVLGTpkGSWVSMGVYSDGS-YNVPPGIIYSYPVTCANGD-WTIVQGLSIDeKSRAKLDATADEL 320 Physcomitrella ...
ABB30235     237 SAMSAAKAAVDHVHDWIHGTpeGVYVSMGVYSDGNpYGVPSDLIFSFPCTCHAGE-WTVVSDKLNGdLGKQRLASTIAEL 315 Leishmania mexi...
P93819       243 SALSAASSACDHIRDWVLGTpeGTFVSMGVYSDGS-YSVPSGLIYSFPVTCRNGD-WSIVQGLPIDeVSRKKMDLTAEEL 320 thale cress
AAK83037     240 SALSAAKAIVDHVHDWMLGTpsGTHVSMAVYSDGNpYGVPGGLIFSFPVTCSGGE-WQIVSGLNVTpAISERIKATTTEL 318 Trypanosoma brucei
ABK24750     291 SALSAASSACDHIRDWVLGTpkGTWVSMGVYSDGS-YDVPPGIIYSYPVTCENGS-WSIVQGLPINeFSRKKLDATANEL 368 Sitka spruce
XP_001014922 278 SVMSAANAVKDHLHDWYFGTksGEFVSMAVYSDGS-YGVPKGLIFSYPVTCSNFN-YKIVQGLNIDeFSKEKIRITTEEL 355 Tetrahymena the...
CAC80842     245 SAMSAANAVGDHLHDWLLGS--DRIVSMSVASDGS-YGIEKGTIFSFPLRCSRGGtYEIVKGLQLDdFSQRYIKITADEL 321 Galdieria sulph...
XP_001434590 264 SAASAASAVCDHIHDWLIGTdnGTFVSMGVVTDGKlYGIKEQVCFSFPCICKDGN-VKVVEGLKWDqFQQSMIDKTLKEL 342 Paramecium tetr...
XP_001015159 239 SVMSAAIAIRDHLVNWIYGTpeGQFVSMGVYTDGSfYDIPADFVFSVPVKCKDFK-YEVVKDLTISqFSRQKINISLREL 317 Tetrahymena the...
Feature 1                
5MDH_A       320 AEEKETAF 327 pig
XP_001778242 321 VEEKALAY 328 Physcomitrella patens subsp. patens
ABB30235     316 QEERAQAG 323 Leishmania mexicana
P93819       321 KEEKDLAY 328 thale cress
AAK83037     319 EEERREVS 326 Trypanosoma brucei
ABK24750     369 VEEKALAY 376 Sitka spruce
XP_001014922 356 LSEKADAF 363 Tetrahymena thermophila SB210
CAC80842     322 YAEKAEAL 329 Galdieria sulphuraria
XP_001434590 343 LEEKEMAF 350 Paramecium tetraurelia strain d4-2
XP_001015159 318 QDEKEEAN 325 Tetrahymena thermophila SB210

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