1JSW,1K7W,1C3U,1I0A,1XWO,1HY0,1TJ7,1AOS,1DOF,1Q5N,1YIS,1RE5,1FUO,1J3U,1YFE,1FUQ,1YFM,1VDK


Conserved Protein Domain Family
Lyase_I
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cd01334: Lyase_I 
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Lyase class I family; a group of proteins which catalyze similar beta-elimination reactions.
The Lyase class I family contains class II fumarase, aspartase, adenylosuccinate lyase (ASL), argininosuccinate lyase (ASAL), prokaryotic-type 3-carboxy-cis,cis-muconate cycloisomerase (pCMLE), and related proteins. It belongs to the Lyase_I superfamily. Proteins of this family for the most part catalyze similar beta-elimination reactions in which a C-N or C-O bond is cleaved with the release of fumarate as one of the products. These proteins are active as tetramers. The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits.
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Statistics
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PSSM-Id: 176461
Aligned: 325 rows
Threshold Bit Score: 146.108
Created: 14-Jul-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitestetramer
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active sites [active site]
Evidence:
  • Comment:The four active sites of the homotetrameric enzyme are each formed by residues from three different subunits.
  • Structure:1K7W_A; duck delta 2 crystallin mutant tetramer with bound argininosuccinate, contacts at 4 A
  • Structure:1FUO_A: Escherichia coli, fumarase C bound to citrate and a water molecule, contacts at 3.5 A
  • Citation:PMID 8909293
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                       
1JSW_A       48 VRGMVMVKKAAAMANKELQtipksVANAIIAacdevlnngkcmdqfpvdvyqggagtsvnmnTNEVLANiglelmghqkg 127 Escherichia coli
1K7W_A       37 SEVDIQGSMAYAKALEKAGiltktELEKILSglekiseewskgv-----fvvkqsdedihtaNERRLKEligdi------ 105 Anas platyrhynchos
NP_283639    32 AEWDIQGSLAHAQMLKETGvldegDLADIRRgmaeileeirsgk-----iewssdledvhmnIERRLTDkigda------ 100 Neisseria mening...
ZP_00667242  32 YRYDIEGSIAHCRMLAQCKiishdDASLIVQglgeirrelergk-----lhlgssnedihmaIEQELMRkigev------ 100 Syntrophobacter ...
Q7TUI1       31 ILEDIECSIAHAKMLSKTKvlstdESLKIIEgletikekfiegk-----fcpgapsedihycIEEKLINliget------ 99  Prochlorococcus ...
NP_487927    33 IEYDLTGSQAHAQMLAHTGiispaEGEQLVAgleqireeyrqgk-----fqpgidaedvhfaVERRLTEivgdv------ 101 Nostoc sp. PCC 7120
O67383       31 AFEDIEQDIAHVKTLQKAGilteeEARELIQellkikeeikegk-----fqwkkeledvhmnIEAELINrlgdv------ 99  Aquifex aeolicus
YP_361068    30 YEEDIRGSIAHVTMLARQGiitneEKEKIIGalteileeikagk-----vdfspeaedihlnIETLLIKkigdv------ 98  Carboxydothermus...
AAN87483     32 YKQDIRGSIAHARMLGRQGiiaasEAEQIIEglekimadieagr-----vefdvaaedihmnIEKILTErigdv------ 100 Heliobacillus mo...
NP_951218    33 YHQDIRGSIAHARMLGKQGilpmaEVEKIVAglqevlarieagk-----fdfsvaledihmnIEARLTEkigea------ 101 Geobacter sulfur...

Feature 1                       ##                                            ##                
1JSW_A      128 eyqylnpndhvNKCQSTNDAYPTGFRIAVYSSLikLVDAINQLREGFERKAvefqDILKMGRTQLQDAVPMTLGQEFRAF 207 Escherichia coli
1K7W_A      106 -------agklHTGRSRNDQVVTDLKLFMKNSLsiISTHLLQLIKTLVERAaieiDVILPGYTHLQKAQPIRWSQFLLSH 178 Anas platyrhynchos
NP_283639   101 -------gkrlHTGRSRNDQVATDIRLWLRDQItvIQSLIQSLQTALLDLAeqnaETVMPGFTHLQVAQPVSFGHHMLAY 173 Neisseria mening...
ZP_00667242 101 -------ggklHTARSRNDQVALDVRLYMRDTLlqCRGLILQTQKVLVSCAeenlGVVMPGFTHLQHAQPILLSHHLMAY 173 Syntrophobacter ...
Q7TUI1      100 -------gkklHTGRSRNDQVGTDIRLWLRKKIdnIDILLYELQNSLFSIAesniYTLIPGYTHMQRAQPLSLAHHLLAY 172 Prochlorococcus ...
NP_487927   102 -------gkklHTARSRNDQVGTDTRLYLRDQIlqIKTQLREFQRVLLDIAeknvETLIPGYTHLQRAQPVSLAHHLLAY 174 Nostoc sp. PCC 7120
O67383      100 -------grklHTARSRNDQVATDEKLYLKKEIkeVLQLLKELRKTLVELAettvDFVMPSYTHLQRAQPIRVAHYFLAY 172 Aquifex aeolicus
YP_361068    99 -------gkkvHTGRSRNDQVALDTRLYVKKEGtaIIALIKELQETLINLAeghlNTIMPGYTHLQRAQPVTLAHHLLAY 171 Carboxydothermus...
AAN87483    101 -------gkklHTGRSRNDQVALDVRLYLRDEIteTGRLLRELITVLLERAeehvDTIMPGYTHLQKAQPITLAHHLLAY 173 Heliobacillus mo...
NP_951218   102 -------gkrlHTGRSRNDQVALDIRLYLRDEIveISAYLDMLVDSLIYQAeanlGVIMPGYTHLQTAQPILFSHHMMAY 174 Geobacter sulfur...

Feature 1                                                                                       
1JSW_A      208 SILLKEEVKNIQRTAellleVNLgatAIGTglntpkeySPLAVKKLAevtgfpcVPAEdlieatsdCGAYVMVHGALKRL 287 Escherichia coli
1K7W_A      179 AVALTRDSERLGEVKkrinvLPLgsgALAGnpl--didREMLRSELEf-----aSISLnsmdaiseRDFVVEFLSFATLL 251 Anas platyrhynchos
NP_283639   174 VEMLGRDNERMADCRrrvnrMPLgaaALAGtty--pirREITAELLGf-----eQICQnsldavsdRDFAVEFTAAASLI 246 Neisseria mening...
ZP_00667242 174 YEMLKRDDERFEQCFhrtnvLPLgsaALAGttf--pidMEWTAKYLNf-----pRVTSnsidavsdRDYLIEFGAASAML 246 Syntrophobacter ...
Q7TUI1      173 LEMFQRDRERLKEVRarvniSPLgaaALAGtki--kidRYFTAEELGf-----gNIYKnsidavsdRDFCIEFASSSALI 245 Prochlorococcus ...
NP_487927   175 FQMAQRDWERLGDVYrrvniSPLgcgALAGttf--pidRHYTAELLQf-----eRIYEnsldgvsdRDFAIEFLCAASLI 247 Nostoc sp. PCC 7120
O67383      173 REILLKDSERLMDTYrrvdeLPLgsgAVAGvdf--pldRFYTAELLGf-----nRVTRnsmyataeRDFIIEFLSNCALI 245 Aquifex aeolicus
YP_361068   172 FWMFDRDRSRFYDCLkradrSPLgagALAGttl--pldREFVSELLGf-----nGVCEnsldavsdRDYILEFLFAAATT 244 Carboxydothermus...
AAN87483    174 VQMFGRDCSRLVDCAkrfniSPLgsgALAGttf--pldRMAVAQELGf-----dGITEnsldgvsdRDFAIEFCGAASLI 246 Heliobacillus mo...
NP_951218   175 VEMFTRDKGRMEDCLrrmnvLPLgagALAGttf--pidREHVAELLDf-----pGVTRnsldsvsdRDFALEFMGASSIL 247 Geobacter sulfur...

Feature 1                                                 #                                     
1JSW_A      288 AVKMSKICNDLRLLSSgpragLNEINLPel-qaGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTMAaeagqlqlnvmep 366 Escherichia coli
1K7W_A      252 MIHLSKMAEDLIIYSTs---eFGFLTLSdafstGASLMPQKKNPDSLELIRSKAGRVFGRLASILMVlkglpstynkdlq 328 Anas platyrhynchos
NP_283639   247 MVHLSRLSEELILWMSp---rFGFIDIAdrfctGSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLmksqplaynkdnq 323 Neisseria mening...
ZP_00667242 247 MMHVSRLAEELILWSSt---eFDFIEISdafctGSSIMPQKKNPDVPELMRGKTGRVYGNLMALLTLtkalplaynrdlq 323 Syntrophobacter ...
Q7TUI1      246 MSHLSRISEEIILWVTd---eFSFAKLTdkcatGSSLMPQKKNPDVPELIRGKTGRVYGHLQSLLTMikgvplsynkdfq 322 Prochlorococcus ...
NP_487927   248 MVHLSRLSEEIILWASe---eFRFVILKdscatGSSIMPQKKNPDVPELVRGKTGRVFGHLQAMLVImkglplaynkdlq 324 Nostoc sp. PCC 7120
O67383      246 AQHLSRLAEDLIIWNTe---eFNFVELPdklctGSSIMPQKKNPDVLELIRGKTGRIYGNLIALLTTmkalpmaynrdmq 322 Aquifex aeolicus
YP_361068   245 MMHLSRFSEEIVLWNSk---eFSFVEIDdryatGSSMMPQKKNPDAAELIRGKTGRVYGNLMAVLTMmkglplaynkdmq 321 Carboxydothermus...
AAN87483    247 MMHLSRFCEEIILWSSq---eFSFIELDdaystGSSMMPQKKNPDVAELIRGKTGRVYGDLMALLTVmkslplaynkdmq 323 Heliobacillus mo...
NP_951218   248 MMHLSRFSEELILWSTs---eFKFVELTdsfctGSSIMPQKKNPDVPELVRGKTGRVYGNLMALLTVmkalplaynkdmq 324 Geobacter sulfur...

Feature 1                                      
1JSW_A      367 VIGqAMFESVHILTNACYNLLekcingITAN 397 Escherichia coli
1K7W_A      329 EDKeAVFDVVDTLTAVLQVATgvi-stLQIS 358 Anas platyrhynchos
NP_283639   324 EDKePLFDTADTLIDTLRIYAdmm-rgVTVK 353 Neisseria meningitidis Z2491
ZP_00667242 324 EDKePVFDTADTIISTLRLLSrli-peIRFH 353 Syntrophobacter fumaroxidans MPOB
Q7TUI1      323 EDKePIFDTVDTISSCLKAMTillnegIEFN 353 Prochlorococcus marinus subsp. pastoris str. CCMP1986
NP_487927   325 EDKeGLFDSVNTVKACLEAMTillqegLEFR 355 Nostoc sp. PCC 7120
O67383      323 EDKePLFDTLKNLKNMIKGMTlvl-sdLRVK 352 Aquifex aeolicus
YP_361068   322 EDKePLFDTVDTLKGSLRVFTgml-ktIKFN 351 Carboxydothermus hydrogenoformans Z-2901
AAN87483    324 EDKeNLFDAVDTVKGCLAVFTpmi-rtMRVR 353 Heliobacillus mobilis
NP_951218   325 EDKePLFDTIDTVKGSLKIFAdmv-reMRIN 354 Geobacter sulfurreducens PCA

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