1M7J


Conserved Protein Domain Family
D-aminoacylase

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cd01297: D-aminoacylase 
Click on image for an interactive view with Cn3D
D-aminoacylases (N-acyl-D-Amino acid amidohydrolases) catalyze the hydrolysis of N-acyl-D-amino acids to produce the corresponding D-amino acids, which are used as intermediates in the synthesis of pesticides, bioactive peptides, and antibiotics.
Statistics
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PSSM-Id: 238622
Aligned: 22 rows
Threshold Bit Score: 339.273
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active siteputative
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1M7J; Alcaligenes faecalis D-aminoacylase with bound metal ions
  • Comment:The enzyme binds two zinc ions with widely different affinities, although only the tightly bound zinc ion is required for activity.
  • Comment:some proteins in this group lack the conserved histidines which bind a second zinc ion

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                   # #                 
1M7J_A       10 DYILSGGTVIDGtNAPGRLADVGVRGDRIAAVGDlsa--ssaRRRIDVAGKVVSPGFIDSHTHDDnyllkhRDMTPKISQ 87  Alcaligenes faec...
NP_937226    37 DTVILNGRVIDPeTKFDAIANIGIKDNQIVTITTdai---sgDKTIDATGKVVAPGFIDVHAHGQn----iGDYRMQAMQ 109 Vibrio vulnificu...
NP_762644    62 DIVIENGRVVDPeTGLDAIRNVGIKGKRIAAISTntl---kgAKVINAEGLVVAPGFVDLHAHGQq----lPAARAQAFD 134 Vibrio vulnificu...
ZP_00108140   3 DLLIQNGLIFDGlGSLPVRGDIGIQNGRIVTLAPslt--tpaREVVDASGLWVTPGFIDIHTHYDleleiaPGLSESVRH 80  Nostoc punctiforme
ZP_00095882  10 DILIRNGTVVDGtGAPAFKADVRVRDGVIAEVGEnls--pngERVFDASGCHVTPGFIESHTHYDgtmwwqPDLDPLPGY 87  Novosphingobium ...
Q10830       17 DVIIRDGLWFDGtGNAPLTRTLGIRDGVVATVAAgaldetgcPEVVDAAGKWVVPGFIDVHTHYDaevlldPGLRESVRH 96  Mycobacterium bovis
ZP_00095062   5 DLIIRNGTICDGtGAPRFTGDVAVKNGLIVAVGAvrg---daAEEVDATGRIVAPGWVDVHTHYDgqatwdPEMAPSSWH 81  Novosphingobium ...
ZP_00095867  15 DLLIRDGTVIDGsGAAPFTGDVAIKDDRIVHVGPafr--gsaARTIDAQGLIVTPGFVDIHTHYDgqaawsDTLSPSSSH 92  Novosphingobium ...
ZP_00094524   5 DIVLREGTVVDGtGADPFVADVAISGGRIAAIGPnls---rgTTEINASGQIVTPGFVDLHTHYDgqvtwdNRLAPSSYH 81  Novosphingobium ...
ZP_00028063  10 DLVIRGGSVIDGlGGPAIETDIGIIGGRIAEIGSist---egREEIDARGQIVTPGFVDIHTHYDgqamweSRFLPSSWH 86  Burkholderia fun...
Feature 1                #                                                                      
1M7J_A       88 GVTTVVTGNCGISLAPlahanppapldl-------------ldeggsfrfarfSDYLEALRAApp--aVNAACMVGHSTL 152 Alcaligenes faec...
NP_937226   110 GVTTMLELESGVLPISswydeqgkkg-----------------lplnygaaagWTYARIATFSgt--qPEATADYFQKAQ 170 Vibrio vulnificu...
NP_762644   135 GVTTQLEMESGLLPISkfyddtakeg-----------------rplnygasvaWTYARVMAKEptmpaADGTIVWFQKAF 197 Vibrio vulnificu...
ZP_00108140  81 GVTSVVIGNCSLSVAIaepqiladifqrvetls---hrliakwlqksvswqtpAEYLQHLQQLpl--gPNVAPMFGHSAL 155 Nostoc punctiforme
ZP_00095882  88 GATTMILGNCGFSPAPlhkympaqremigifsffedipegpfmqnlpwdwnkwSEYRASVERNvk-vpLNYAAYVGHIAI 166 Novosphingobium ...
Q10830       97 GVTTVLLGNCSLSTVYansedaadlfsrveavp---refvlgalrdnqtwstpAEYIEAIDALpl--gPNVSSLLGHSDL 171 Mycobacterium bovis
ZP_00095062  82 GVTTVIMGNCGVGFAPaapdrhdwliglmegved--ipgtalaegmawdwesfPEYLDSLEKMpr--aVDVGTHVPHGAV 157 Novosphingobium ...
ZP_00095867  93 GVTTAVLGNCGVGFAPckpedrealirlmegved--ipgvvmaeglpwdwdsfPSYLDALAARrr--dIDVACLLPHSPL 168 Novosphingobium ...
ZP_00094524  82 GVTTAVIGNCGVGFAPcrrdaasrdamvrlmegvediphpvlteglpwtwetfPDYLDFLASRty--dIDVAAYVPHAPL 159 Novosphingobium ...
ZP_00028063  87 GVTTVVMGNCGVGFAPvrvqdrdrlielmegved--ipgialhegldwqwegfASFMAALERRph--dIDFCAHVPHAAL 162 Burkholderia fun...
Feature 1                                                                                       
1M7J_A      153 RAAVMpdlr-----reatADEIQAMQALADDALASGAIGISTGAfyppa-------------ahasTEEIIEVCRPLITh 214 Alcaligenes faec...
NP_937226   171 SRNDWkmd-------iatPEQQEKILKLIEQGLDEGGLGIGINAgyap---------------ghgQKEYYALAELAAQr 228 Vibrio vulnificu...
NP_762644   198 SYNNWqnt-------latPDELKQILNDIEQGLKEGGLGIGINAgyap---------------gygHKEYYELAKLAKRy 255 Vibrio vulnificu...
ZP_00108140 156 RAHVMglersl--tvqptKFELNLMQRIARDAIDAGFIGISIDMfpwhrmsgewq-gytipsqhakFKEYAMLADLCRRc 232 Nostoc punctiforme
ZP_00095882 167 RLAAMgveaw---ereatAEEIAKMAELLDDALAAGALGMSDNMhdhdgqdr------pvptlkanDAEFEALFDVMERy 237 Novosphingobium ...
Q10830      172 RTAVLgldratddtvrptEAELAKMAKLLDEALEAGMLGMSGMDaaidkldgdrfrsralpstfatWRERRKLISVLRHr 251 Mycobacterium bovis
ZP_00095062 158 RAYVLgerekp--gavptAEDIRKMSEIVEEGVRAGALGFSTSRtvihksvdg----evvpgttatAEELIEIGRAMGRv 231 Novosphingobium ...
ZP_00095867 169 RVWVMgeraia--reeatEADLAEMRRLAREALDAGAVGFATSRlnihrtksg----dliptfgadTRELVAIAGALADa 242 Novosphingobium ...
ZP_00094524 160 RVYVMgqrgad--repatQTDIAEMCRLLEQALDRGALGIATSRslfhrssdg----taiptyqaaQAELMAFAEVLRAk 233 Novosphingobium ...
ZP_00028063 163 RVYVMgaraar--lematPEDVAEMRRLTAEAVRAGAIGVSTSRsinhksvkg----dptpslraaENELLGLALGVKDa 236 Burkholderia fun...
Feature 1              #                                   #                                    
1M7J_A      215 -GGVYATHMRdeg----ehiVQALEETFRIGREL--DVPVVISHHKVMgkln-----fgrSKETLALIEAAmas-qdVSL 281 Alcaligenes faec...
NP_937226   229 -DVATYTHVRyasnaepqssFEAVKELIGNAAIT--GAHMHLCHINSTsl--------kdIDTILPMVEKAkeqginVTV 297 Vibrio vulnificu...
NP_762644   256 -NMPTYTHVRylnsaepkssFEAYQELISLSATT--GAHMHICHLNSTsv--------qdIDDCADLVQSAidrglnVTA 324 Vibrio vulnificu...
ZP_00108140 233 -DRVFQVTPNlq-------rLTSFVDILRMGSGIg-QKPLRLTVLSALda---------vHDRNLWRIFSPll---fIWN 291 Nostoc punctiforme
ZP_00095882 238 pGCCYQVIVDtf------mrMTGPANLERLSKLLa-GRKIKVQIAGAIptlefqkgilpaMQESVRKMREAgvdvwpGYA 310 Novosphingobium ...
Q10830      252 -GRILQSAPDvd------npVSALLFFLASSRIFnrRKGVRMSMLVSAdaksmp-lavhvFGLGTRVLNKLlg--sqVRF 321 Mycobacterium bovis
ZP_00095062 232 gHGVFEMASDmk------reWDEFGWMGALSRET--GLPVTYAALQSIake-------mpLEEQIAEMRHQnaqganIVA 296 Novosphingobium ...
ZP_00095867 243 eTGVFQAVLDapf----tawDEEMGRLLAVAEAA--GRPATFTLGVANsga-------anWKPATDLVDAGrargleIWP 309 Novosphingobium ...
ZP_00094524 234 gKGVFQIVEDih------vpGASLDNMRELARTS--GRPLTFSIGTGNtgp-------ygYPRLLDELAAAnaeglvMKG 298 Novosphingobium ...
ZP_00028063 237 gRGVLQMIMDfddp---teiHSDFAMMRRLVEQS--GRPLSFSLMQKHgnt-------dgWRKLLALTEEAnrdglqMRA 304 Burkholderia fun...
Feature 1                                                                                       
1M7J_A      282 DAYPYVAGStmlkqdrvllagrtlitwckpypelsgrdl----------------------------------------- 320 Alcaligenes faec...
NP_937226   298 GAYPYGAAStvvgaamfqgegwrermgstaenfqlgkermsekqlad--------------------------------- 344 Vibrio vulnificu...
NP_762644   325 EAYPYGAGSsligaevfrgddwlarwgvpsasymeangkpltqekite-------------------------------- 372 Vibrio vulnificu...
ZP_00108140 292 RLLNGNVRFqtltepftiysdgsvtplfeefstgaqlngcqsreerqqlwaseifrrqfrqewlskrrk---sfhrqldl 368 Nostoc punctiforme
ZP_00095882 311 HVSPTSTLSlvkslifaqsndyvwhevvleddhakkaalladpewrarareswdtqawdhsplkn-----------pqel 379 Novosphingobium ...
Q10830      322 QHLPVPFELysdgidlpvfeefgagtaalhlrdqlqrnelladrsyrrsfrrefdriklgpslwhrdf------hdaviv 395 Mycobacterium bovis
ZP_00095062 297 QIALRGNGVimawrgtihpfrfrpsyaaiaelpweeqlvqlkdpafrakvlaeanvypdsdivnlfrmiaegwhnqfimd 376 Novosphingobium ...
ZP_00095867 310 QVLPRPIGMisgwalsthpfclcpsyqaiaglpldeqlptlrdptfraklisevpqpghplamltriw-----dwmfpfn 384 Novosphingobium ...
ZP_00094524 299 QLMPRGIGMilgfeltlnpfyttstfarlaplplaerleqlrrpeiraailsepmdpdpalvlgravrd---fdhmfllg 375 Novosphingobium ...
ZP_00028063 305 QVAPRGIGVllglqtsrnifsecptyrkfaneplasriaqmrkpeirealiaelvatetglgkrlsef-----dnifafg 379 Burkholderia fun...
Feature 1                                                                                #      
1M7J_A      321 -----------------------eeiaaergkskydvvpelqpagaiyfmmdEPDVQRILaf----gpTMIGSDGLphd- 372 Alcaligenes faec...
NP_937226   345 ---------------yqannpgtfivwhfldennpedlakldasilspdvliESDEMFWMhmdeeghvDNYAGDEWplpk 409 Vibrio vulnificu...
NP_762644   373 --------------lqandpgkvvvmhflkpddnpsdrqkmdrsvlfpgaaiASDAMPWMds----kgQVIEGDIWplps 434 Vibrio vulnificu...
ZP_00108140 369 mevihcpeaswqglsfaeiarqnqqepvdlfihalqkydtdlrwvatgandrLKPRLAMMqhp---hiLPGFTDAGa--- 442 Nostoc punctiforme
ZP_00095882 380 flldsengagplnitlkeyadslglhrsdamadwilkngtrstvhmapfpkdEALTLELMkdp---ktVGNISDAGa--- 453 Novosphingobium ...
Q10830      396 ecpdksligksfgaiaderglhpldafldvlvdngernvrwttivanhrpnqLNKLAAEPs------vHMGFSDAGa--- 466 Mycobacterium bovis
ZP_00095062 377 edfdyepqpeasillraktagvspdafaydklmedegngfiylpilnyadgnLDFLEALQaad---dtVNSLSDGGa--- 450 Novosphingobium ...
ZP_00095867 385 dppqyeparetsiaaqaraqgrsceevaydllmeregngmilntlgnflegkLDALLELMrre---dtVIGLGDGGa--- 458 Novosphingobium ...
ZP_00094524 376 ddpdyeqppersiagrareagitpeelaydvmtegesggllylamanyadgsLDAVGDILshp---dvVLGLGDGGa--- 449 Novosphingobium ...
ZP_00028063 380 dladydpgedasitaisrqqnrspeqvaydltianegrgilyspfanyafrnLDACAEMMesp---ytVLGLGDGGa--- 453 Burkholderia fun...
Feature 1                                                                                       
1M7J_A      373 -erpHPRLWGT---FPRVLGHYSRDlgl-----fpLETAVWKMTGLTAAKFGLa-----eRGQVQPGYYADLVVFDPATV 438 Alcaligenes faec...
NP_937226   410 dlfsHPRSNGT---FAKVLRVYVREral-----lsMSEAIRKMTLMPAQLLEDfvpqmknKGRIQPNMDADIVVFDPETI 481 Vibrio vulnificu...
NP_762644   435 daiaHPRSLAC---FTRFISKYVNEyka-----vtLIEAMERCSLNACRIIENsvpqmknKGRVQVGKDADLVIFKLEDI 506 Vibrio vulnificu...
ZP_00108140 443 ----HVRNLGYy-dGALSLLKQAVAtkf-----lsPEAAICRVTGEPAGWFCLd------TGVVKVGAKADIVLLDPNAL 506 Nostoc punctiforme
ZP_00095882 454 ----HLQMLCGggeNALLLTQYVREekk-----lsLEQAIHVMTGKLAGHFNLn-----dRGVIAVGKRADIAVFNMDEI 519 Novosphingobium ...
Q10830      467 ----HLRNMAFy-nFGLRLLKRARDadragqpflsIERAVYRLTGELAEWFGIg------AGTLRQGDRADFAVIDPTHL 535 Mycobacterium bovis
ZP_00095062 451 ----HCGTICDaasPTFMLQHWVRDrrrg--srisLEHAIKRQCHDTARLYGLd-----dRGVIAPGYLADINVIDFDRV 519 Novosphingobium ...
ZP_00095867 459 ----HYAAICDasyPTFMLTYWVRDrage---rltLPEAVERLAARPARVMGLe-----dRGLLKPGYKADLNVIDLDRL 526 Novosphingobium ...
ZP_00094524 450 ----HVGTICDasySTFALCHWARDrqrg---rktVADMVHRMTQATARVIGLe-----dRGELAVGKRADINVIDLAQL 517 Novosphingobium ...
ZP_00028063 454 ----HVAMISDssfQTFVLTHYGRGgl------fdLPHLVKRQTSDTARMIGLl-----dRGHLAPGMKADLNVIDMNAL 518 Burkholderia fun...
Feature 1                                                                     
1M7J_A      439 ADSATfe--------------hPTERAAGIHSVYVNGAAVWedq---------sftGQHAGR 477 Alcaligenes faecalis
NP_937226   482 SDVGTyq--------------dPNHPAVGVHWLLVNGEIVVnnse--------ldtTVKAGK 521 Vibrio vulnificus YJ016
NP_762644   507 KVLATfe--------------aPNALSEGMHHVIVNGTPIIange--------lqlGVNPGE 546 Vibrio vulnificus CMCP6
ZP_00108140 507 NQPISpqvqisdpildgeprmvKRGSDEIVQAVYINGVRIVcgg----------evSDRLGR 558 Nostoc punctiforme
ZP_00095882 520 QRREMekafdvpd-grggttwrFTRQAMPTRLTLVNGVPTFeng---------aftGAMPGK 571 Novosphingobium aromaticivorans
Q10830      536 DESVDgyheeavp-yygglrrmVNRNDATVVATGVGGTVVFrggqfggqfrdgygqNVKSGR 596 Mycobacterium bovis
ZP_00095062 520 RLLKPwlafdl-----paggkrLLQKAEGYDYTIKGGKVTFrng---------ewtGATPGN 567 Novosphingobium aromaticivorans
ZP_00095867 527 TLHAPvvkhdl-----pgggrrLDQTATGYVATVVNGRVIRehd---------qptDERPGR 574 Novosphingobium aromaticivorans
ZP_00094524 518 ALRPPqvchdl-----paggrrLIQRATGYSLTMLAGEIVLrdd---------eptGLLPGR 565 Novosphingobium aromaticivorans
ZP_00028063 519 EIQPPhmfhdl-----pagggrLLQNANGYVATIVSGVPVYrhg---------katGALPGK 566 Burkholderia fungorum

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