3JVV,2EWV,2GSZ,2EWW,2EYU,5FL3


Conserved Protein Domain Family
PilT
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cd01131: PilT 
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Pilus retraction ATPase PilT
Pilus retraction ATPase PilT is a nucleotide-binding protein responsible for the retraction of type IV pili, likely by pili disassembly. This retraction provides the force required for travel of bacteria in low water environments by a mechanism known as twitching motility.
Links
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Statistics
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PSSM-Id: 410875
Aligned: 41 rows
Threshold Bit Score: 284.045
Created: 17-Aug-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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ATP bindinghexamer
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:3JVV; Pseudomonas aeruginosa PilT with bound ATP, contacts at 4A
    - View structure with Cn3D
  • Comment:contains Walker A and Walker B motifs
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1           #                      ########                        #                  
3JVV_A    103 VLTMEELGMGEVFKRVSDvp-rGLVLVTGPTGSGKSTTLAAMLDYLNNTkyHHILTIEDPIEFVHeskKCLVNQREVHRD 181 Pseudomonas aerugi...
NP_635985 105 IPSIEELNLPQVLKDVIMtp-rGLVLVVGSTGSGKSTSLASMIDHRNSTttGHILTIEDPIEYLHkhkMSIVNQREVGLD 183 Xanthomonas campes...
NP_230117 101 IPTFEELKLPEVLQNLAIak-rGLVLVVGATGSGKSTTMAAMTGYRNQHrtGHILTVEDPIEFVHehkRCIVTQREVGLD 179 Vibrio cholerae O1...
P52052    100 CPQLEQLGAPTVLPELLKse-nGLILVTGATGSGKSTTLAAMVGYLNQHadAHILTLEDPVEYLYasqRCLIQQREIGLH 178 Escherichia coli K-12
NP_273116 139 IPQMQELGLPEKLKDLAVap-rGLLIIVGPTGSGKSTTMATMLEHRNKTlpSHIVTIEDPIEFIYkprRCIFTQREIGVD 217 Neisseria meningit...
NP_869319 142 IPDFRGLFLPDSIENLCHye-qGMVLLAGVTGSGKSTTIGSMLNYINSIyrKHILTLEDPIEFIFtedKSLINQREIGQD 220 Rhodopirellula bal...
ACO70878  110 MRNFDDLNLPPQIADLASkq-rGIVIIGGATGSGKSTTLAAMVEHVNTSvrKHIVTIEDPIEFLFtdkESIIEQREIGID 188 uncultured Verruco...
ABM28812  104 IPSLAHLNLHPSLEEFTRlr-sGLILVCGATGSGKTTTMAAMLDEINRNrpAHIITLEDPIEYRFaskKSFVEQRELGRH 182 Desulfovibrio vulg...
EIJ67115   93 FLPEFPRELYTVLEDLYLsn-sGLVLVTGSTGSGKSTTLRFFLEEYNRKhsKKIICLEDPIEFYYqeqKSLFFQREIGRD 171 Fusobacterium necr...
NP_602429  92 PIKLKGKFINKLIDEKILflkdGLVLVTGITGSGKSTTLANIIEKFNKNknLKILTIEDPIEYIFenkKSLIIQREIGED 171 Fusobacterium nucl...

Feature 1                                                    #                                
3JVV_A    182 tlgFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTsAAKTIDRVVDVFPaeeKAMVRSMLSESLQ 261 Pseudomonas aerugi...
NP_635985 184 thaFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNnADQTIERILNFFPesaHKNVLMNLALNLR 263 Xanthomonas campes...
NP_230117 180 tesYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANnANQALERILHLVPkeqREQFLLDLSLNLK 259 Vibrio cholerae O1...
P52052    179 cmtFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRgAAQAVERLVDSFPaqeKDPVRNQLAGSLR 258 Escherichia coli K-12
NP_273116 218 tinWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANtAPQSLERILNFYPkeqHNQILIDIALNLT 297 Neisseria meningit...
NP_869319 221 vvdFSIGMKHAVREDPDIILVGELRDEETFMTAIHAAETGHLVFGTIHAAsASTCIGRILDLFPeemHNAIRSAIAFNMK 300 Rhodopirellula bal...
ACO70878  189 tatYSSGLRHVLRQDPDVIVIGEMRDAESAHAAMSAANIGRLVFTTLHTAdTIQSVQRVLEFFPsseRDFARRLFAETLT 268 uncultured Verruco...
ABM28812  183 fpsFERGLLDVLREDPDVVLVGELREAETIRLALSAAESGHLIIASLHSSsPEEALYRLCNAFPleaQDLVRHQLSSSLN 262 Desulfovibrio vulg...
EIJ67115  172 sesFQTAMKSALRQDPDILLIGEIRDLESLQSALYFAESGHLVFSTLHTGnCVESIHKILSLSPkekQEEVRQRLLSSLR 251 Fusobacterium necr...
NP_602429 172 vesFEKALKSSLRQDPDVIILGEIRDEESLYSALKLAETGHLVFSTLHTMnTVESVNRLISMVRsekKDFIREQLASVLR 251 Fusobacterium nucl...

Feature 1           #         # #                                              
3JVV_A    262 SVISQTLIKKIg--gGRVAAHEIMIGTPaIRNLIREdkvAQMYSAIqtggslGMQTLDMCLKGLV 324 Pseudomonas aeruginosa
NP_635985 264 AVVSQRLVKGLd--gRRRPATEVLLNTPmIRDLLRRgqvHEVKQAMeesleeGMQTFDQCLFRMV 326 Xanthomonas campestris pv. campes...
NP_230117 260 GVIAQQLLRDKng-kGRHGVFEVLLNSPrIADLIRRgelHELKATMarsqevGMQTFDQALYQLV 323 Vibrio cholerae O1 biovar El Tor ...
P52052    259 AVLSQKLEVDKq--eGRVALFELLINTPaVGNLIREgktHQLPHVIqtgqqvGMITFQQSYQHRV 321 Escherichia coli K-12
NP_273116 298 GIICQRLALKQdk-tGRTAVVDLLINTPaIQDFILKgdlMNISKIMetaktdGMQTMDQNLFELY 361 Neisseria meningitidis MC58
NP_869319 301 GIVAQKLLKSIkpgvSRVPTCEVMTFSPmIRKLVLEghdNKLPDAIrigaedGMQDFTMSLKQLI 365 Rhodopirellula baltica SH 1
ACO70878  269 GVVCQRLVRSEq--sGTIPAAEVLLNSPsIARMIAEehlDKIRGALelggpdGMQTFDQHLNELV 331 uncultured Verrucomicrobia bacterium
ABM28812  263 AIVVQQLQMHPta-rFRVPVLSILVGNNpVRMLVREgkfAQLTSLMemgrgdGMFTADAYRTEFL 326 Desulfovibrio vulgaris subsp. vul...
EIJ67115  252 WMIAQELVRGKe--kGRIALFELLKNTKaVANMIYSgreLQLPSILessfsqGMCTKEKSREYWV 314 Fusobacterium necrophorum subsp. ...
NP_602429 252 FIFSQELHRGK----KTVPIFEILNNTKaVANLILNnklNQIPTLIesgienFMITKEKYLKNLE 312 Fusobacterium nucleatum subsp. nu...

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