1JBG


Conserved Protein Domain Family
HTH_TipAL-Mta

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cd01106: HTH_TipAL-Mta 
Click on image for an interactive view with Cn3D
Helix-Turn-Helix DNA binding domain of the transcription regulators TipAL, Mta, and SkgA
Helix-turn-helix (HTH) TipAL, Mta, and SkgA transcription regulators, and related proteins, N-terminal domain. TipAL regulates resistance to and activation by numerous cyclic thiopeptide antibiotics, such as thiostrepton. Mta is a global transcriptional regulator; the N-terminal DNA-binding domain of Mta interacts directly with the promoters of mta, bmr, blt, and ydfK, and induces transcription of these multidrug-efflux transport genes. SkgA has been shown to control stationary-phase expression of catalase-peroxidase in Caulobacter crescentus. These proteins are comprised of distinct domains that harbor an N-terminal active (DNA-binding) site and a regulatory (effector-binding) site. The conserved N-terminal domain of these transcription regulators contains winged HTH motifs that mediate DNA binding. These proteins share the N-terminal DNA binding domain with other transcription regulators of the MerR superfamily that promote transcription by reconfiguring the spacer between the -35 and -10 promoter elements. Unique to this family, is a TipAL-like, lineage specific Bacilli subgroup, which has five conserved cysteines in the C-terminus of the protein.
Statistics
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PSSM-Id: 133381
View PSSM: cd01106
Aligned: 79 rows
Threshold Bit Score: 85.2304
Threshold Setting Gi: 21205598
Created: 30-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
DNA bindingdimer interface
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:DNA binding residues [nucleic acid binding site]
Evidence:
  • Comment:Based on sequence similarity to BmrR and the structure of Bacillus subtilis BmrR bound to DNA (1EXI).

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1        ###             #                ###                                           
1JBG_A        3 YQVKQVAEISGVSIRTLHHYDNIELLnPSALTDaGYRLYSdADLERLQQILFFKEIGFRLDEIKEMLDHpn------fDR 76  Bacillus subtilis
YP_094652     4 YKIKEISKLTSLSIRTLQYYDEINLLkPSKRSQsDYRLYSsDDVLRLQQITTLRYLGCSLASIKEIIQHpd------fNL 77  Legionella pneum...
YP_126760     4 WFVKDLSQLTGVSVQTLHHYDRIGLLkPSLRLAnGYRVYSeKDLLKLQQIIALKFFGFELSQIKTLLAEe-------gNA 76  Legionella pneum...
YP_140018     8 FSTGELAKEAEVSIRTVQYYDQRGILtPSKVTEgGRRIYHeSDLERLKVICFLRDLDFSINQIKNLLQEenr----eqVL 83  Streptococcus th...
NP_269856     5 YSTGELANLAGVSIRTVQYYDQRGILiPTALTAgGRRLYTdSDLEQLRMICFLRDLGFSIEQIRKVLAEenaaqvlelLL 84  Streptococcus py...
NP_736227     5 YSTGDLAKEAGVTVRTVQYYDKRGILsPSELSEgGRRVYSiADLEKLRQIIYLRDLDFSIDNIKNLFTEdnasqilelFL 84  Streptococcus ag...
NP_965807     5 YTIGEAAKKLQVSTRTLRFYEEKDLVrPAYTEEnGYRFYEkDQIRQLELILFLKELGFSLKQIKMLIQDehg----nqSV 80  Lactobacillus jo...
YP_194780     5 YTIGELAKKLNITTRTIRYYDQKGLVrPNSIGEnGYRFYTvEQVKQLKLVIFLKELGFSLKDIQKILEDeng----dkLI 80  Lactobacillus ac...
NP_694240     4 YSTGEVAKMLGVSVRSLRYYDQIGLIsPNKKDEyGKRFYSdDDIVTLKKITILKMSNLSLDNIKKILSEi--------TI 75  Oceanobacillus i...
ZP_01169488   4 WTTGEAAKQRNVSVRTLRYYDQIGLLaPSFKDEnGKRHYSeEDLFTLEKILILKSLSLPLEEIREVLKQl--------SY 75  Bacillus sp. NRR...
Feature 1                                    
1JBG_A       77 KAALQSQKEILMKKKQRMDEMIQTIDRTL 105 Bacillus subtilis
YP_094652    78 KTSLAVQAKALEKKAEQIHEASSLLNYIA 106 Legionella pneumophila subsp. pneumophila str. Philadelphia 1
YP_126760    77 LNHFNSQARVLEQKASTLLEGAKTLRRII 105 Legionella pneumophila str. Lens
YP_140018    84 ELLLTDQIESLEKSSKEIEVKLKRARHLQ 112 Streptococcus thermophilus LMG 18311
NP_269856    85 VDHIATAKEDLAAKEQQVDIAVKILDRLR 113 Streptococcus pyogenes M1 GAS
NP_736227    85 QVQIRELRLAIDSKKDKLDKAVNLLKTVE 113 Streptococcus agalactiae NEM316
NP_965807    81 ELLLKEQYQENQRKIEEISKKQAQIKHLQ 109 Lactobacillus johnsonii NCC 533
YP_194780    81 ELLLKDQLKRNKEETKKLEKQNKKIIQLQ 109 Lactobacillus acidophilus NCFM
NP_694240    76 EQLLYVHRDYLQQKIEELHASVHHTTALL 104 Oceanobacillus iheyensis HTE831
ZP_01169488  76 KQILVSHYNHLQEQLSELQTSISNTASLI 104 Bacillus sp. NRRL B-14911

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