1B26,1BVU,1L1F,1V9L


Conserved Protein Domain Family
NAD_bind_1_Glu_DH

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cd01076: NAD_bind_1_Glu_DH 
Click on image for an interactive view with Cn3D
NAD(P) binding domain of glutamate dehydrogenase, subgroup 1
Amino acid dehydrogenase (DH) is a widely distributed family of enzymes that catalyzes the oxidative deamination of an amino acid to its keto acid and ammonia with concomitant reduction of NADP+. Glutamate DH is a multidomain enzyme that catalyzes the reaction from glutamate to 2-oxyoglutarate and ammonia in the presence of NAD or NADP. It is present in all organisms. Enzymes involved in ammonia assimilation are typically NADP+-dependent, while those involved in glutamate catabolism are generally NAD+-dependent. Amino acid DH-like NAD(P)-binding domains are members of the Rossmann fold superfamily and include glutamate, leucine, and phenylalanine DHs, methylene tetrahydrofolate DH, methylene-tetrahydromethanopterin DH, methylene-tetrahydropholate DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains. These domains have an alpha -beta-alpha configuration. NAD binding involves numerous hydrogen and van der Waals contacts.
Statistics
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PSSM-Id: 133445
View PSSM: cd01076
Aligned: 50 rows
Threshold Bit Score: 230.501
Threshold Setting Gi: 25028133
Created: 30-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) binding
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                    ###                    ##        
1B26_F    180 GGSKGREEATGRGVKVCAGLAMDVlg--------idPKKATVAVQGFGNVGQFAALLISQelGSKVVAVSDSrGGIYNPE 251 Thermotoga maritima
1V9L_F    180 WGNPVREYATGFGVAVATREMAKKlw--------ggIEGKTVAIQGMGNVGRWTAYWLEKm-GAKVIAVSDInGVAYRKE 250 Pyrobaculum island...
Q53199    165 GGIAGRAESTGRGVQFAIQSSLRDtrtpgl-dgrrnLKGASTVIQGFGNVGYHAARFLSEedDARVTVLAERdGYVANPE 243 Rhizobium sp. NGR234
XP_637204 217 GGIRGRTEATGLGVFYGIREFLSYeevlkktgltpgIKGKSIVIQGFGNVGYFAAKFFEQa-GAKVIAVAEHnGAVYNAD 295 Dictyostelium disc...
P00368    209 GGIHGRISATGRGLFGHIENFIENasymsilgmtpgFGDKTFAVQGFGNVGLHSMRYLHRf-GAKCVAVGEFdGSIWNPD 287 chicken
AAG15493  174 GGISGRTESTGLGVFYGCREILEDyefctqagipagLRGKNIIIQGYGAVGYYAAKYMCAy-GAKLVGVAEWdGSIYEEN 252 Paramecium tetraur...
NP_502267 241 GGIHGRVSATGRGVWKGLEVFTNDadymkmvgldtgLAGKTAIIQGFGNVGLHTHRYLHRa-GSKVIGIQEYdCAVYNPD 319 nematode
AAH63940  247 GGIHGRISATGRGVFHGIENFINEasymsklgltpgFADKTFIIQGFGNVGLHSMRYLHRy-GAKCVGIAEIdGSIWNPN 325 zebrafish
P54385    254 GGIHGRVSATGRGVFHGLENFINEanymsqigttpgWGGKTFIVQGFGNVGLHTTRYLTRa-GATCIGVIEHdGTLYNPE 332 fruit fly
XP_789257 249 GGIHGRISATGRGVYHGVENFVNEasymsavsltpgLGDKSFIVQGFGNVGLHSTRYLHRy-GAKCVGVMEIdGSIYNPA 327 purple urchin
Feature 1                                                      ##                   ###       
1B26_F    252 GFDvEELIRYKKEHGtvvtypkg-------eritneeLLELDVDILVPAALEgAIHAGNAERIKAKAVVEGANGPTTPEA 324 Thermotoga maritima
1V9L_F    251 GLN-VELIQKNKGLTgpalvelfttkdnaefvknpdaIFKLDVDIFVPAAIEnVIRGDNAGLVKARLVVEGANGPTTPEA 329 Pyrobaculum island...
Q53199    244 GLSiEALKQHQIRTGsilgfdga------ksiagdmcGVEQPCDVLIPAAMEnAIHAENAERMKAHLVVEAANGPVTFEA 317 Rhizobium sp. NGR234
XP_637204 296 GLNiDALNKYKLQHGtfidfpga------tnivdsvkALEIPCDILIPAALEkQIHIGNVADIQAKLIGEAANGPMTPRA 369 Dictyostelium disc...
P00368    288 GIDpKELEDYKLQHGtimgfpk--------aqklegsILETDCDILIPAASEkQLTKANAHKVKAKIIAEGANGPTTPQA 359 chicken
AAG15493  253 GIDpDELQAFKEQRKgvkgfpka------seyhedesVIYKECDIFIPAAFEqTVNRANAPKFNCKVIAEAANGPTTLAA 326 Paramecium tetraur...
NP_502267 320 GIHpKELEDWKDANGtiknfpga------knfdpfteLMYEKCDIFVPAACEkSIHKENASRIQAKIIAEAANGPTTPAA 393 nematode
AAH63940  326 GMDpKELEDYKLQHGtivgfpn--------sqpyegnILEAQCDILIPAAGEkQLTRKNAHNIKAKIIAEGANGPTTPDA 397 zebrafish
P54385    333 GIDpKLLEDYKNEHGtivgyqna-------kpyegenLMFEKCDIFIPAAVEkVITSENANRIQAKIIAEAANGPTTPAA 405 fruit fly
XP_789257 328 GIHpKELEDYKTEHGtivgfpg--------aepyegnLLTAQCDILVPCAGEkQITADNAHDIKAKIIAEGANGPTTPAA 399 purple urchin
Feature 1                                                                                     
1B26_F    325 DEILSRRG-ILVVPDILANAGGVTVSYFEWvqdLQSFFWdld-------------------------------------- 365 Thermotoga maritima
1V9L_F    330 ERILYERG-VVVVPDILANAGGVIMSYLEWvenLQWYIWdee-------------------------------------- 370 Pyrobaculum island...
Q53199    318 DEILRSRG-VTILPDLYVNAGGVVVSYFERvknLTHIPFglmerrrrergnhtiatalermt------------------ 378 Rhizobium sp. NGR234
XP_637204 370 DQILLNRG-HVIIPDLLLNAGGVTVSYFEWlknLSHVRFgrlnkkweesskklllefvestv------------------ 430 Dictyostelium disc...
P00368    360 DKIFLERN-IMVIPDLYLNAGGVTVSAFZZk-nLNHVSYgrltfkyerdsnyhllmsvqeslerkfg------------- 424 chicken
AAG15493  327 EEILIKKG-VKFLPDILLNAGGVTVSYLQW---LQNLDHirpgrmtrrweetskyklleaiqist--------------- 387 Paramecium tetraur...
NP_502267 394 DRILLARGdCLIIPDMYVNSGGVTVSYFEWlknLNHVSYgrltfkydeeankmllasvqeslskav-------------- 459 nematode
AAH63940  398 DKIFIERN-VMVIPDMYLNAGGVTVSYFEWlknLNHVSYgrltfkyerdsnyhllmsvqeslerkfg------------- 463 zebrafish
P54385    406 DKILIDRN-ILVIPDLYINAGGVTVSFFEWlknLNHVSYgrltfkyeresnyhllasvqqsieriindesvqes-lerrf 483 fruit fly
XP_789257 400 DAILLSKN-VLVIPDMFINAGGVTVSYFEWlknLNHVSYgrltfkyerdsnthllqsvqeslerkfgrhesvqeslerkf 478 purple urchin
Feature 1                                                                           
1B26_F    366 --------------------------qvrNALEKMMKGAFNDVMKVkeky--nvdMRTAAYILAIDRVAY 407 Thermotoga maritima
1V9L_F    371 --------------------------etrKRLENIMVNNVERVYKRwqre-kgwtMRDAAIVTALERIYN 413 Pyrobaculum islandicum
Q53199    379 ------gkespadmrdefleggaeidlvrSGLEDVMRSTWTRIADLmeqqpelgdYRTAAYVASIRQVAD 442 Rhizobium sp. NGR234
XP_637204 431 ------nkklseaersliihgadeidivrSGLEDTMQNACAETRKTanek--ntdYRSAALYNAIMKIKA 492 Dictyostelium discoideum AX4
P00368    425 -khggtipvvptaefqdrisgasekdivhSGLAYTMERSARQIMRTamkynlgldLRTAAYVNAIEKVFK 493 chicken
AAG15493  388 ---glrvdvtknqqaakllegpsakdlvfTGLEESMAVAVQNTKETaskl--nisLRMAGYYNALMTIHQ 452 Paramecium tetraurelia
NP_502267 460 ---gkdcpvepnaafaakiagasekdivhSGLEYTMQRSGEAIIRTahkynlgldIRTAAYANSIEKVYN 526 nematode
AAH63940  464 -kqggpipivptadfqarvagasekdivhSGLAYTMERSARQIMRTankynlgldLRTAAYVNAIEKVFK 532 zebrafish
P54385    484 grvggripvtpsesfqkrisgasekdivhSGLDYTMERSARAIMKTamkynlgldLRTAAYVNSIEKIFT 553 fruit fly
XP_789257 479 grhgsgipitpsdqfkdriqgasekdivhSGLSYTMERSARQIMKTamkynlgldIRTAAYVNSIEKIFL 548 purple urchin

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