1NPD,1NPY,1NVT,1NYT,1P74,1WXD,2D5C,2EGG,2HK9


Conserved Protein Domain Family
NAD_bind_Shikimate_DH

?
cd01065: NAD_bind_Shikimate_DH 
Click on image for an interactive view with Cn3D
NAD(P) binding domain of Shikimate dehydrogenase
Shikimate dehydrogenase (DH) is an amino acid DH family member. Shikimate pathway links metabolism of carbohydrates to de novo biosynthesis of aromatic amino acids, quinones and folate. It is essential in plants, bacteria, and fungi but absent in mammals, thus making enzymes involved in this pathway ideal targets for broad spectrum antibiotics and herbicides. Shikimate DH catalyzes the reduction of 3-hydroshikimate to shikimate using the cofactor NADH. Amino acid DH-like NAD(P)-binding domains are members of the Rossmann fold superfamily and include glutamate, leucine, and phenylalanine DHs, methylene tetrahydrofolate DH, methylene-tetrahydromethanopterin DH, methylene-tetrahydropholate DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DHs, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains. These domains have an alpha-beta-alpha configuration. NAD binding involves numerous hydrogen and van der Waals contacts.
Statistics
?
PSSM-Id: 133443
View PSSM: cd01065
Aligned: 72 rows
Threshold Bit Score: 97.3419
Threshold Setting Gi: 21219999
Created: 13-Aug-2003
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
NAD(P) bindingshikimate
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                               #  #                    ##                            
1NPD_A    106 TDGTGHIRAIKESgfdikGKTXVLLGAGGASTAIGAQGAieg-lkEIKLFNRRdeffDKALAFAQRVnent---dcvvTV 181 Escherichia coli
AAC01719  108 FDGTGFVAGLVEAghdpkARRVSLVGAGGAGSAIAAAVLaag-adRLALCDPDt---AKLAALKGRLdvhwpgrtsvsAE 183 Amycolatopsis medi...
NP_248935 110 TDCLGFAEGFRRGladapRQRVVQMGAGGAGAAVAHALLaeg-veRLVLFDVDp---ARAQALADNLnqhfg-apralAG 184 Pseudomonas aerugi...
NP_522957 112 TDWWGFAESFKRGlpgapMGQVVQLGAGGAGAAVAHAMLtlg-aqSLTLFDVEp---ERARQLAASLcgrfg-pgraqAG 186 Ralstonia solanace...
Q9X5C9    109 TDVSGFGRGMEEGlpnakLDSVVQVGAGGVGNAVAYALVthg-vqKLQVADLDt---SRAQALADVInnavg--reavVG 182 Corynebacterium gl...
AAN33246  108 LDGVGFVRGLANAghdvkGRSVYLAGAGGAASAIAFALAeag-vvRLTVVNRSs---EKAGQLVARLkehf----pagMF 179 Brucella suis 1330
Q9HS68     99 TDVAGVKRAFAHHdvslsGAQAVVVGAGGAGRAAAFALAda--gaTVRIANRTr---AAADELAADVggt------avGL 167 Halobacterium sali...
NP_770183 108 LDARGFVASLAQHqiavrGRKVLQVGAGGAGRATAFGLAeag-arELTIMNRDg---AKAHALAEAVsrr-----ypeTK 178 Bradyrhizobium jap...
NP_770300 111 VDGLGFVGAVRANgydptGKCALLVGAGGAGSAIAMALVeag-vrSLAIHDNEa---ARRDQLIAKLatlgk-agigpGS 185 Bradyrhizobium jap...
Q8KBH8    107 TDIDGFAAPLLPMaesirNRPVCIFGNGGAALAAVEAFRlrfnpsSVLLVVRDt---QKAEDMLEEYayrdlvtihagRE 183 Chlorobaculum tepidum
Feature 1                          ###                                  #                     
1NPD_A    182 TDladqqafaealasaDILTNGTKVgxkp---lenESLVndisl-----lhpglLVTECVYNPHxtkLLQQAQQAGcKTI 253 Escherichia coli
AAC01719  184 PDta----------gaDLVVNATPLglr----pgdPLPFrpdrl------apgrVVADIIMKPRetrLLREAAALG-HDV 242 Amycolatopsis medi...
NP_248935 185 SDlaga------lgdaQGLVNTTPVgma----klpGMPVpaell------radlWVAEVIYFPLeteLLRQARALGcRTL 248 Pseudomonas aerugi...
NP_522957 187 TDlaaa------maaaDGLVHATPTgma----ghpGLPLppdll------qsrhWVAEIVYFPLeteLLRRARALGcRTL 250 Ralstonia solanace...
Q9X5C9    183 VDargied---viaaaDGVVNATPM----------GMPAhpgtafdvscltkdhWVGDVVYMPIeteLLKAARALGcETL 249 Corynebacterium gl...
AAN33246  180 VSqgtp-------aghDIIVNGTSLglk----egdALPVdpqyl------rpemLVAEVIMSPEvtpLLQIAKDSGcSIH 242 Brucella suis 1330
Q9HS68    168 GDlprs------ladaTVLVHATTVgmd----dpdTSPVsadal------hddlAVLDAVYSPVetrLLRDAAAAGaTTI 231 Halobacterium sali...
NP_770183 179 VNagrae-----arafDLIVNTTSLgmk----tddPLPLdiegv------gpatAVSDIIVTPEvtrLLEQAAAQGaRTI 243 Bradyrhizobium jap...
NP_770300 186 ADps----------gfELVANATPLgmk----agdPLPLdaskl------kastFVGCVITSPAvspLVEAARRLGcKTS 245 Bradyrhizobium jap...
Q8KBH8    184 IDqpacsk---lirdcRVLVNATPVgtagrndhihSILPtghgl-----lhdgqIVYDMVYNPPetpLLAEARAAGaTVI 255 Chlorobaculum tepidum
Feature 1      #  ##             
1NPD_A    254 DGYGXLLWQGAEQFTLWTG 272 Escherichia coli
AAC01719  243 HYGSHMLAGQLDSYRDFFG 261 Amycolatopsis mediterranei
NP_248935 249 DGGTMAVFQAVKAFELFSG 267 Pseudomonas aeruginosa PAO1
NP_522957 251 DGGGMAVFQAVGAFELFTG 269 Ralstonia solanacearum GMI1000
Q9X5C9    250 DGTRMAIHQAVDAFRLFTG 268 Corynebacterium glutamicum
AAN33246  243 PGKAMLDGQLAEMFDFFTR 261 Brucella suis 1330
Q9HS68    232 DGAWMLLYQGAEAFERWTG 250 Halobacterium salinarum
NP_770183 244 GGKPMLDAQMALVARFIAR 262 Bradyrhizobium japonicum USDA 110
NP_770300 246 TGTEMYNALQSSMVDFLLA 264 Bradyrhizobium japonicum USDA 110
Q8KBH8    256 AGIEMLIAQAARAFSIWTG 274 Chlorobaculum tepidum

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap