1MTY,1MHZ,2INN,2INC,3DHG


Conserved Protein Domain Family
AAMH_B

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cd01058: AAMH_B 
Click on image for an interactive view with Cn3D
Aromatic and Alkene Monooxygenase Hydroxylase, subunit B, ferritin-like diiron-binding domain
Aromatic and Alkene Monooxygenase Hydroxylases, subunit B (AAMH_B). Subunit B (beta) of the soluble hydroxylase of multicomponent, aromatic and alkene monooxygenases are members of a superfamily of ferritin-like iron-storage proteins. AAMH exists as a hexamer (an alpha2-beta2-gamma2 homodimer) with each alpha-subunit housing one nonheme diiron center embedded in a four-helix bundle. The N-terminal domain of the alpha- and noncatalytic beta-subunits possess nearly identical folds; the beta-subunit lacks the C-terminal domain found in the alpha-subunit. Methane monooxygenase is a multicomponent enzyme found in methanotrophic bacteria that catalyzes the hydroxylation of methane and higher alkenes (as large as octane). Phenol monooxygenase, found in a diverse group of bacteria, catalyses the hydroxylation of phenol, chloro- and methyl-phenol and naphthol. Both enzyme systems consist of three components: the hydroxylase, a coupling protein and a reductase. In the MMO hydroxylase, dioxygen and substrate interact with the diiron center in a hydrophobic cavity at the active site. The reductase component and protein coupling factor provide electrons from NADH for reducing the oxidized binuclear iron-oxo cluster to its reduced form. Reaction with dioxygen produces a peroxy-bridged complex and dehydration leads to the formation of complex Q, which is thought to be the oxygenating species that carries out the insertion of an oxygen atom into a C-H bond of the substrate. The toluene monooxygenase systems, toluene 2-, 3-, and 4-monooxygenase, are similar to MMO but with an additional component, a Rieske-type ferredoxin. The alkene monooxygenase from Xanthobacter strain Py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol. Alkane omega-hydroxylase (AlkB) and xylene monooxygenase are members of a distinct class of integral membrane diiron proteins and are not included in this CD.
Statistics
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PSSM-Id: 153116
View PSSM: cd01058
Aligned: 41 rows
Threshold Bit Score: 289.165
Threshold Setting Gi: 1066147
Created: 24-Sep-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
dimerization
Conserved site includes 29 residues -Click on image for an interactive view with Cn3D
Feature 1:dimerization interface [polypeptide binding site]
Evidence:
  • Structure:1MTY; Methylococcus capsulatus Methane Monooxygenase Hydroxylase (MMOH) alpha, beta, gamma subunits dimer interface.
    View structure with Cn3D
  • Comment:MMOH is composed of two copies each of three subunits; the active site of each alpha subunit contains one diiron center.
  • Comment:Putative reductase binding site: large groove at dimer interface.
  • Citation:PMID 9329079

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       #
1MTY_B        37 RWKRLTEYEaLTVYAQPNadwiaggldwgdwt----------qkfhggrpswgnetTELRTVDWFKHRDPLRRWHAPYVK 106 Methylococcus c...
3DHG_B        18 MRKKPSEYDiVSRKLHYStnnpdspwelspd------------spmnlwykqyrnaSPLKHDNWDAFTDPDQLVYRTYNL 85  Pseudomonas men...
YP_002524205  16 ERRMPSEYElVTHKLNYHfrrqpapfemspd------------wpiqqwylqyregSEFNVDDWERYRDPYAFTYRRYVE 83  Thermomicrobium...
NP_342688     53 GRKKPSEYQvVTYALSYWadprsptyiydkgpfelgtehitqkwykhfrdnspfikPLFERGEWHDYEDPYKLTYWTYNS 132 Sulfolobus solf...
YP_001237714  22 ERRLPSEYEaVTYKFHSHfrrapapfelsen------------wsinqfylrhregSKLMLDDWEGYRDPLTYTYRRYIG 89  Bradyrhizobium ...
CAA09915      22 LGRKPTDYEvVTHNMNHTmrgtplelsptv--------------hanvwlkknrdeIALKVDSWDLFRDPDRTTYDTYVK 87  Xanthobacter sp...
CAB55829      24 LGRRPTEYEvLTHNMNHTtgtvplemgpdv--------------hgnvwlrehrdsMKLTVPDWDAFRDPDTVTYGTYVA 89  Rhodococcus sp....
YP_479333     19 ARRKPNEYEtVTGHFHYHfgrqpapfdldpn------------spinrwylryregSPLRGVDWEEFRDPAQLNYRRYIA 86  Frankia sp. CcI3
AAB58744      19 RRRKPSEYEiVSTNLHFTdrdpnapyelapd------------mwmnrwykqntlnSPLKHDDWNAFRDPDQVIYRTYNL 86  Burkholderia ce...
AAG40798      20 RRRKPSEYEiVSTNLHYTtdnpdapfeldpn------------femaqwfkrnrnaSPLTHPDWNAFRDPDELVYRTYNM 87  Burkholderia ce...
Feature 1        #  ## ##  ##      ## #                                                          
1MTY_B       107 DKAEEWRYTDRFLQGy-SADGQIr-aMNPTWRdEFINRYWGAFLFNEYGLFNAHSQGAREAL--SDVTRVSLAFWGFDKI 182 Methylococcus c...
3DHG_B        86 MQDGQESYVQSLFDQf-NEREHDq-mVREGWE-HTMARCYSPLRYLFHCLQMSSAYVQQMAP--ASTISNCCILQTADSL 160 Pseudomonas men...
YP_002524205  84 ARHERELYLDSLIDDf-EAHDHYr-hLDSTWL-DFLRDYYLPSRFAGHCLQMTGMYVAQMSP--SAYVANPFYFQAADEM 158 Thermomicrobium...
NP_342688    133 MADDNETFLDKIYEEivNTKYDWn--LNEEVL-ELYKNVYDPLRYVFHIMQMESMYLATMAP--TSSIANVFIFMGMDHL 207 Sulfolobus solf...
YP_001237714  90 DQKDREVYCDNLIDEf-ERQDSYr-kLPAAWL-DFLGQTYLPVRFPGHAMQMSAAYVAQMAP--SAFVTNTFYFQMGNEM 164 Bradyrhizobium ...
CAA09915      88 MQDDQETYVDNLLLSy-TGEGRYdeeLSSRSL-DLLSAGLTPTRYLGHGLQMLAAYIQQLAP--SAYVGNCAVFQTSDAL 163 Xanthobacter sp...
CAB55829      90 EQDDQETYVEGLIAQf-DSEKSDe-aLSDEAL-TLLAGALAPTRYVVHGQQMLSAYIQQLAM--SSYVGNCAAFQTADQL 164 Rhodococcus sp....
YP_479333     87 LQHDREIYAEGVVDHf-EALDADa-gLDPVWV-GVLARLYLPARYLFHVLQITSLYVGQLGP--SAYVSNAAFFQAADEL 161 Frankia sp. CcI3
AAB58744      87 MQDGQEAYVYGLFDQfnAREHDKv--LDPRWA-GKLARLYTPARYLFHTLQMASAYVGTDGSlqATITNCQLLSDGLNPF 163 Burkholderia ce...
AAG40798      88 LQDGQETYVFGLLDQfsERGHDAm--LERTWA-GTLARLYTPVRYLFHTLQMGSAYLTQLAP--ASTISNCAAYQTADSL 162 Burkholderia ce...
Feature 1                                                                               #   #   #
1MTY_B       183 DIAQMIQLERGFLAKIVpgfde-stavPKAEWTNGEVYKSARLAVEGLWQevFDWNESAFSVHAVYDALFGQFVRRefFQ 261 Methylococcus c...
3DHG_B       161 RWLTHTAYRTHELSLTYpdag--lgehERELWEKEPGWQGLRELMEKQLTa-FDWGEAFVSLNLVVKPMIVESIFKp-LQ 236 Pseudomonas men...
YP_002524205 159 RRIQRTAYLAKAIAVDTgrddladsqlARARWEDNPLWQPLRELMERHLVv-YDWGAAFAVRNLVVKPLYDALFNDq-LS 236 Thermomicrobium...
NP_342688    208 RRVQRISQRVKMLDIVYpslg--fgkeTRKVFEESPIFQPTREVLEKMLVt-YDVGEALVAFNLAVKFVLDELILQh-LT 283 Sulfolobus solf...
YP_001237714 165 RRVQRQAYLAKALALDTgrpeladsgiARTIWTQGPEWQGLRELIEKQLIt-YDWGEAFVSRNLVLRPIFDHIFNRe-IA 242 Bradyrhizobium ...
CAA09915     164 RRVQRVAYRTRQLADAHparg--fgsgDRAVWEKSPDWQPIRKAIEELLVt-FEWDKALAGTNFVVKPILDELFLNh-LA 239 Xanthobacter sp...
CAB55829     165 RRVQLTAYRTTQLQLTYperg--fgtaEKEIWTEHPDWQPIRAAFEYALVe-FDWDRAFVATNLVTKAIADQLFLVq-LV 240 Rhodococcus sp....
YP_479333    162 RALQWIAYRAKALSLVHgadla-dsrvTRRFWEDDPAWQPAREALERLLVa-YDWAEAFTALNLVVKPALDTLLKTa-LA 238 Frankia sp. CcI3
AAB58744     164 RWWSHTAYRTKELSLAFddks--fgtdERQYWETDPAWQGFRELMEKVLVt-WDWAEAFVAFSLVAIPAVEEAVLRk-LG 239 Burkholderia ce...
AAG40798     163 RWLTHTAYRTKELSQTFsdfg--fgtdERRYWEQDPAWQGWRKLVEHALVa-WDWAECFVALSLVVRPAVEEAVLRs-LG 238 Burkholderia ce...
Feature 1           #     #   #  ## #  ##  #                                                     
1MTY_B       262 RLAPRf-GDNLTPFFINQAQTYFQIAKQGVQDLYynCLGDdpefSDYNRTVMRNWTGKWLEPTIAAL-RDFMGLFaklpa 339 Methylococcus c...
3DHG_B       237 QQAWEn-NDTLLPLLIDSQLKDAERHSRWSKALVk-HALE----NPDNHAVIEGWIEKWRPLADRAA-EAYLSMLss--- 306 Pseudomonas men...
YP_002524205 237 NLARAadDELLALIHDDFQLYDAPYARENTIALVa-YVREr---RPELVDWLRQQVTPWLDLAHAAA-QAIGQAFs---- 307 Thermomicrobium...
NP_342688    284 QPFSKl-GDEMIKHIHLSFYNDTLRHRHQAQELFk-YAFSk---EPSLKDVIKPWVKDWQEMGFKAT-EGFRDVLkg--- 354 Sulfolobus solf...
YP_001237714 243 DLARAn-DDDLLALLHDDFRNYDEAYAVETTKALvrYATGk---MPAHAVLLQEWVAKWIPLGERAA-RSLSEALs---- 313 Bradyrhizobium ...
CAA09915     240 RLLHVe-GDELDSLVLRNLHGDAQRHARWTAALGr-FAVEq---NVNNRTVLRDAIAGWHETGEAVLaAGAGMLAs---- 310 Xanthobacter sp...
CAB55829     241 HQANEl-GARLDSLILENLWRDSQRSQRWSVALVk-FLTAa---DPGNLEVLQGHLNDWSERGDKMIdAGARIIA----- 310 Rhodococcus sp....
YP_479333    239 DLADAn-GDGLTAQLLRESGVDGARSRAWSAALAr-HAIAg---RPDNAAVLAAWEENWRPRAEKAV-AGLAALFdn--- 309 Frankia sp. CcI3
AAB58744     240 ESGQHn-GDTLVGLLNDAQLVDAARHRRWAATLVk-MAVEk--aGQRRTLIKKMGSSKWEPLADSAI-TAYCTALs---- 310 Burkholderia ce...
AAG40798     239 EAARHn-GDTLLGLLTDAQLADAQRHRRWAGALVr-MALE----QPGNREVITGWLAKWEPLADEAI-VAYCSALp---- 307 Burkholderia ce...
Feature 1                     #   ## ###  
1MTY_B       340 gttdkeeitaSLYRVVDDWIEDYAS 364 Methylococcus capsulatus
3DHG_B       307 ---------dILPAQYLERSTSLRA 322 Pseudomonas mendocina
YP_002524205 308 ----------TTGLVSPIDVADYAQ 322 Thermomicrobium roseum DSM 5159
NP_342688    355 ---------eYDNAIRQIRKAHSEY 370 Sulfolobus solfataricus
YP_001237714 314 ----------AAPGADSARTILDRT 328 Bradyrhizobium sp. BTAi1
CAA09915     311 ----------RAPSADAAKIADEVR 325 Xanthobacter sp. Py2
CAB55829     311 ----------SPNGRSAADIADAVR 325 Rhodococcus sp. AD45
YP_479333    310 ---------aPNPASPAEVATTVAA 325 Frankia sp. CcI3
AAB58744     311 ----------DVPDASASARLATRE 325 Burkholderia cepacia
AAG40798     308 ----------EAPAAQARATAAVRE 322 Burkholderia cepacia

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